{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:40:24Z","timestamp":1760971224727,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The unique inborn hypertriglyceridemia seen in FLS (fatty liver Shionogi) mice was relieved by the administration of purified apolipoprotein (apo) C\u2010II. Lipoprotein lipase (LPL) and its cofactor, apoC\u2010II, play a pivotal role in VLDL metabolism. Therefore, we investigated the genetic background involved in this hypertriglyceridemia. Plasma levels of TG and total cholesterol as well as LPL activity were measured in male FLS mice and C57\/BL6J mice. Agarose gel electrophoresis and fast protein liquid chromatography were used to analyze the lipoprotein profile. A cross experiment was done to determine the genetic background of hypertriglyceridemia observed in FLS mice. cDNA sequences of apoC\u2010II and apoC\u2010III of FLS mice were determined. Pre\u03b1\u2010lipoprotein was the predominant lipoprotein class in FLS mouse plasma. LPL activity remained in the range observed in C57\/BL6J mice, and purified apoC\u2010II transiently relieved FLS mice from hypertriglyceridemia. Pre\u03b1\u2010lipoproteinemia was inherited in an autosomal recessive manner. ApoC\u2010III appeared to be a causal factor for this unique hypertriglyceridemia. Microsatellite analysis, however, revealed that the responsible chromosome was not 7; rather, apoC\u2010III mapped onto chromosome 9. Therefore, we suggest apoC\u2010III as a candidate causative factor for the hypertriglyceridemia observed in FLS mice because an excessive amount of apoC\u2010III attenuates LPL activity <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1007\/s11745-003-1116-7","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T10:55:57Z","timestamp":1165402557000},"page":"687-692","source":"Crossref","is-referenced-by-count":3,"title":["A novel type hypertriglyceridemia observed in FLS mice"],"prefix":"10.1002","volume":"38","author":[{"given":"Masaya","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiji","family":"Saibara","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Nemoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoaki","family":"Akisawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Toda","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Tumor Radiology Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Wakatsuki","sequence":"additional","affiliation":[{"name":"Department of Aging and Reproductive Medicine Kochi Medical School Nankoku 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuichiro","family":"Inagaki","sequence":"additional","affiliation":[{"name":"Center for Experimental Animals Development, Aburahi Laboratories Shionogi &amp; Company, Ltd. 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