{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:20:53Z","timestamp":1784949653583,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diarrhea caused by enterotoxigenic <jats:italic>Escherichia coli<\/jats:italic> (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in particular, several gangliosides. Because milk also contains gangliosides, these have been suggested to serve as ligands for bacterial fimbriae and thus prevent the bacterial attachment to mucosa. The most relevant ETEC strains in calves, including those with K99 and F41 fimbriae, were assayed to determine whether they are able to bind gangliosides isolated from several stages of bovine lactation. Both GM3 and GD3, the main gangliosides of milk, were recognized by ETEC strains, although the different fimbriae showed diverse levels of affinity. Unexpectedly, the adhesion to colostral gangliosides was considerably weaker than that to gangliosides from the other stages of lactation. Because the carbohydrate moiety did not change and because differences in the percentages of unsaturated FA and sphingosine between colostrum and other stages were observed, we conclude that the differences in adhesion could be due to a different composition of the ganglioside caramide.<\/jats:p>","DOI":"10.1007\/s11745-003-1124-7","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T10:55:57Z","timestamp":1165402557000},"page":"761-768","source":"Crossref","is-referenced-by-count":25,"title":["Enterotoxigenic <i>Escherichia coli<\/i> strains bind bovine milk gangliosides in a ceramide\u2010dependent process"],"prefix":"10.1002","volume":"38","author":[{"given":"Mar\u00eda\u2010Jes\u00fas","family":"Mart\u00edn","sequence":"first","affiliation":[{"name":"Departmento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Biolog\u00eda Edificio Departmental, lab. 103 Universidad de Salamanca Campus Unamuno Salamanca 37007 (Spain)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Mart\u00edn\u2010Sosa","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Biolog\u00eda Edificio Departmental, lab. 103 Universidad de Salamanca Campus Unamuno Salamanca 37007 (Spain)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefa M.","family":"Alonso","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica, Biolog\u00eda Molecular y Fisiolog\u00eda Universidad de Valladolid, Escuela Universitaria de Ingenier\u00edas Agrarias Soria 42003 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Hueso","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Biolog\u00eda Edificio Departmental, lab. 103 Universidad de Salamanca Campus Unamuno Salamanca 37007 (Spain)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.377"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01288-8"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-440X(95)80054-9"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(98)80105-1"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.58.070189.001521"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.27.1.216-221.1980"},{"key":"e_1_2_2_8_1","first-page":"57","article-title":"Enterotoxins, Colonization Factors and Serotypes of Enterotox\u2010igenic Escherichia coli from Humans and Animals","volume":"7","author":"Blanco J.","year":"1991","journal-title":"Microbiol. Semin."},{"key":"e_1_2_2_9_1","first-page":"259","article-title":"Enterotoxigenic Escherichia coli (ETEC) in Farm Animals","volume":"30","author":"Nagy B.","year":"1999","journal-title":"Vet. Res."},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.10.4518-4522.1993"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-7-1591"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80693-D"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000711450000235X"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/127.5.980S"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.15.2.676-678.1977"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39314-7"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm3.1992.373.1.283"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1971.tb11988.x"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)38019-X"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199501000-00015"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0720-x"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(97)00394-8"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198605000-00008"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01048444"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a124562"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01049678"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.55.5.1216-1223.1987"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.1.332-342.1996"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/8.4.297"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.9.3467-3474.1996"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a124071"},{"key":"e_1_2_2_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)78102-2"},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1071\/AR9930495"},{"key":"e_1_2_2_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1963.tb08933.x"},{"key":"e_1_2_2_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41583-4"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s11745-003-1124-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:15:46Z","timestamp":1760969746000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/s11745-003-1124-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1007\/s11745-003-1124-7"],"URL":"https:\/\/doi.org\/10.1007\/s11745-003-1124-7","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"value":"0024-4201","type":"print"},{"value":"1558-9307","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}