{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:45:51Z","timestamp":1761007551900,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The FA composition of <jats:italic>Fasciola hepatica<\/jats:italic> 12 kDA purified native FA\u2010binding protein (<jats:italic>n<\/jats:italic>Fh12), a candidate vaccine against fascioliasis, is described. The FA chain lengths ranged between 12 and 24 carbons. The principal FA were 16\u22360 18\u22361n\u22129, 18\u22360, 20\u22364n\u22126, and 20\u22361n\u22129. The acids 16\u22360, 18\u22361n\u22129, and 18\u22360 comprised over half the FA that were bound to the whole FA\u2010binding protein. Small amounts (1.0\u20132.8%) of <jats:italic>isoanteiso<\/jats:italic> methyl\u2010branched FA also were characterized. Forty\u2010one different FA were identified in extracts of the adult flukes, with the three most abundant FA also being 16\u22360, 18\u22361n\u22129, and 18\u22360. A similar proportion of saturated vs. unsaturated FA was observed between the whole extract from <jats:italic>F. hepatica<\/jats:italic> and the <jats:italic>n<\/jats:italic>Fh12 protein. However, the n\u22123\/n\u22126 ratio of PUFA was significantly different, being 1.2 in the whole extract vs. 9.6 in the <jats:italic>n<\/jats:italic>Fh12 protein complex. The <jats:italic>n<\/jats:italic>Fh12 protein binds more n\u22125, n\u22126, and n\u22127 PUFA and less n\u22123 and n\u22129 PUFA than the whole extract. In addition, cholesterol (56%), sitosterol (36%), and fucosterol (8%) also were bound to the <jats:italic>n<\/jats:italic>Fh12 protein complex.<\/jats:p>","DOI":"10.1007\/s11745-003-1125-6","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T10:55:57Z","timestamp":1165402557000},"source":"Crossref","is-referenced-by-count":2,"title":["Fatty acids bound to <i>Fasciola hepatica<\/i> 12 kDa fatty acid\u2010binding protein, a candidate vaccine, differ from fatty acids in extracts of adult flukes"],"prefix":"10.1002","volume":"38","author":[{"given":"N\u00e9stor M.","family":"Carballeira","sequence":"first","affiliation":[{"name":"Dept. of Chemistry University of Puerto Rico P.O. Box 2334600931\u20103346 San Juan Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidyleen","family":"Cruz","sequence":"additional","affiliation":[{"name":"Dept. of Chemistry University of Puerto Rico P.O. Box 2334600931\u20103346 San Juan Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George V.","family":"Hillyer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, School of Medicine University of Puerto Rico Medical Sciences Campus San Juan Puerto Rico 00936\u20105067"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,7]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1985.34.1127"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1987.37.362"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761995000200021"},{"key":"e_1_2_2_5_1","first-page":"377","volume-title":"Fasciolosis","author":"Spithill T.W.","year":"1999"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00096-9"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2001)087[0426:IOFAMI]2.0.CO;2"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(75)90173-3"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(88)90128-8"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2001)087[1028:IAICOF]2.0.CO;2"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01411414"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(91)90244-C"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)92995-9"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(92)90202-L"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90043-W"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46634-8"},{"key":"e_1_2_2_17_1","first-page":"2568","article-title":"Can We Vaccinate Against Schistosomes?","volume":"25","author":"Hillyer G.V.","year":"1976","journal-title":"Fed. 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