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We employed gene microarray analysis to find the network of genes involved in GC\u2010evoked cell death, using three clones derived from the CEM lymphoid leukemia cell line. Clone C1\u201315 was resistant to GC\u2010evoked apoptosis, although not necessarily to GC\u2010induced gene transcription; the other two underwent apoptosis in the presence of GC. Clone C7\u201314 was subcloned from the apoptosis\u2010sensitive parental C7 clone to establish karyotypic uniformity. The second sensitive clone, C1\u20136, was a spontaneous revertant from parental resistant clone C1. A period of \u226524 h in the constant presence of receptor\u2010occupying concentrations of synthetic GC dexamethasone (Dex) was necessary for apoptosis to begin. To identify the steps leading to this dramatic event, we identified the changes in gene expression in the 20\u2010h period preceding the onset of overt apoptosis. Cells in the log phase of growth were treated with 10<jats:sup>\u22126<\/jats:sup> M Dex, and 2\u201320 h later, mRNA was prepared and analyzed using the Affymetrix HG_U95Av2 chip, containing probes for about 12,600 genes. Of these, approximately 6,000 were expressed above background. Comparisons of the basal and expressed genes in the three clones led to several conclusions: The dex\u2010sensitive clones shared the regulation of a limited set of genes. The apoptosis\u2010resistant clone C1\u201315 showed Dex effects on a largely different set of genes. Promoter analysis of the regulated genes suggested that primary gene targets for GC often lack a classic GC response element.<\/jats:p>","DOI":"10.1007\/s11745-004-1302-7","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T11:57:55Z","timestamp":1516622275000},"page":"821-825","source":"Crossref","is-referenced-by-count":14,"title":["Identification of genes leading to glucocorticoid\u2010induced leukemic cell death"],"prefix":"10.1002","volume":"39","author":[{"given":"E. B.","family":"Thompson","sequence":"first","affiliation":[{"name":"Department of Human Biological Chemistry &amp; Genetics University of Texas Medical Branch 301 University Boulevard Galveston TX 77555\u20101068"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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