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Accordingly, techniques to examine in vivo shifts in fatty acid metabolism are of value in both clinical and experimental settings. Partially metabolizable long chain fatty acid (LCFA) tracers have been recently developed and employed for this purpose: [9,10\u2010<jats:sup>3<\/jats:sup>H]\u2010(<jats:italic>R<\/jats:italic>)\u20102\u2010bromopalmitate ([<jats:sup>3<\/jats:sup>H]\u2010BROMO) and [<jats:sup>125<\/jats:sup>I]\u201015\u2010(\u03c1\u2010iodophenyl)\u20103\u2010<jats:italic>R<\/jats:italic>,<jats:italic>S<\/jats:italic>\u2010methylpentadecanoic acid ([<jats:sup>125<\/jats:sup>I]\u2010BMIPP). These analogues are taken up like native fatty acids, but once inside the cell do not directly enter \u03b2\u2010oxidation. Rather, they become trapped in the slower processes of \u03c9 and \u03b1\u2010oxidation. Study aims were to (1) simultaneously assess and compare [<jats:sup>3<\/jats:sup>H]\u2010BROMO and [<jats:sup>125<\/jats:sup>I]\u2010BMIPP and (2) determine if tracer breakdown is affected by elevated metabolic demands. Catheters were implanted in a carotid artery and jugular vein of Sprague\u2013Dawley rats. Following 5 days recovery, fasted animals (5 h) underwent a rest (<jats:italic>n<\/jats:italic> = 8) or exercise (<jats:italic>n<\/jats:italic> = 8) (0.6 mi\/h) protocol. An instantaneous bolus containing both [<jats:sup>3<\/jats:sup>H]\u2010BROMO and [<jats:sup>125<\/jats:sup>I]\u2010BMIPP was administered to determine LCFA uptake. No significant difference between [<jats:sup>125<\/jats:sup>I]\u2010BMIPP and [<jats:sup>3<\/jats:sup>H]\u2010BROMO uptake was found in cardiac or skeletal muscle during rest or exercise. In liver, rates of uptake were more than doubled with [<jats:sup>3<\/jats:sup>H]\u2010BROMO compared to [<jats:sup>125<\/jats:sup>I]\u2010BMIPP. Analysis of tracer conversion by TLC demonstrated no difference at rest. Exercise resulted in greater metabolism and excretion of tracers with \u223c37% and \u223c53% of [<jats:sup>125<\/jats:sup>I]\u2010BMIPP and [<jats:sup>3<\/jats:sup>H]\u2010BROMO present in conversion products at 40 min. In conclusion, [<jats:sup>3<\/jats:sup>H]\u2010BROMO and [<jats:sup>125<\/jats:sup>I]\u2010BMIPP are indistinguishable for the determination of tissue kinetics at rest in skeletal and cardiac muscle. Exercise preferentially exacerbates the breakdown of [<jats:sup>3<\/jats:sup>H]\u2010BROMO, making [<jats:sup>125<\/jats:sup>I]\u2010BMIPP the analogue of choice for prolonged (&gt;30 min) experimental protocols with elevated metabolic demands.<\/jats:p>","DOI":"10.1007\/s11745-008-3183-4","type":"journal-article","created":{"date-parts":[[2008,5,14]],"date-time":"2008-05-14T09:05:02Z","timestamp":1210755902000},"source":"Crossref","is-referenced-by-count":12,"title":["Long Chain Fatty Acid Uptake In Vivo: Comparison of [<sup>125<\/sup>I]\u2010BMIPP and [<sup>3<\/sup>H]\u2010Bromopalmitate"],"prefix":"10.1002","volume":"43","author":[{"given":"Jane","family":"Shearer","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Vanderbilt University Nashville TN USA"},{"name":"Departments of Kinesiology, Biochemistry and Molecular Biology University of Calgary 2500 University Drive NW Calgary AB T2N 1N4 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly R.","family":"Coenen","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Vanderbilt University Nashville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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