{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:04:20Z","timestamp":1791187460328,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,1,15]],"date-time":"2011-01-15T00:00:00Z","timestamp":1295049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201008\u2010JCJC\u20100025\u201001"],"award-info":[{"award-number":["ANR\u201008\u2010JCJC\u20100025\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As lipid deposition tissue in fish, the white adipose tissue (WAT) has important functions related to reproduction and the challenges of long\u2010term fasting. In the study reported here, we infused fish fed a high\u2010carbohydrate diet with two doses of insulin for 5 days in order to explore the effects of this hormone on lipogenesis and beta\u2010oxidation\u2010related enzymes. We demonstrated the presence of some of the main lipogenic enzymes at molecular, protein and activity levels (ATP\u2010citrate lyase and fatty acid synthase). However, while ATP\u2010citrate lyase was unexpectedly down\u2010regulated, fatty acid synthase was up\u2010regulated (at protein and activity levels) in an insulin dose\u2010dependent manner. The main enzymes acting as NADPH donors for lipogenesis were also characterized at biochemical and molecular levels, although there was no evidence of their regulation by insulin. On the other hand, lipid oxidation potential was found in this tissue through the measurement of gene expression of enzymes involved in \u03b2\u2010oxidation, highlighting two carnitine palmitoyltransferase isoforms, both down\u2010regulated by insulin infusion. We found that insulin acts as an important regulator of trout WAT lipid metabolism, inducing the final stage of lipogenesis at molecular, protein and enzyme activity levels and suppressing \u03b2\u2010oxidation at least at a molecular level. These results suggest that WAT in fish may have a role that is important not only as a lipid deposition tissue but also as a lipogenic organ (with possible involvement in glucose homeostasis) that could also be able to utilize the lipids stored as a local energy source.<\/jats:p>","DOI":"10.1007\/s11745-010-3521-1","type":"journal-article","created":{"date-parts":[[2011,1,14]],"date-time":"2011-01-14T08:22:36Z","timestamp":1294993356000},"page":"189-199","source":"Crossref","is-referenced-by-count":55,"title":["Insulin Stimulates Lipogenesis and Attenuates Beta\u2010Oxidation in White Adipose Tissue of Fed Rainbow Trout"],"prefix":"10.1002","volume":"46","author":[{"given":"S.","family":"Polakof","sequence":"first","affiliation":[{"name":"INRA, UMR1067 Nutrition Aquaculture et G\u00e9nomique, P\u00f4le d'hydrobiologie, CD918 Saint\u2010P\u00e9e\u2010sur\u2010Nivelle 64310 France"},{"name":"Laboratorio de Fisiolox\u00eda Animal, Departamento de Biolox\u00eda Funcional e Ciencias da 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