{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:33:06Z","timestamp":1761708786534,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,9,2]],"date-time":"2012-09-02T00:00:00Z","timestamp":1346544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F31DK084812"],"award-info":[{"award-number":["F31DK084812"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The weight loss supplement conjugated linoleic acid (CLA) consists of an equal mixture of <jats:italic>trans<\/jats:italic>\u201010,<jats:italic>cis<\/jats:italic>\u201012 (10,12) and <jats:italic>cis<\/jats:italic>\u20109,<jats:italic>trans<\/jats:italic>\u201011 (9,11) isomers. However, high levels of mixed CLA isomers, or the 10,12 isomer, causes chronic inflammation, lipodystrophy, or insulin resistance. We previously demonstrated that 10,12 CLA decreases de novo lipid synthesis along with the abundance and activity of stearoyl\u2010CoA desaturase (SCD)\u20101, a \u03b4\u20109 desaturase essential for the synthesis of monounsaturated fatty acids (MUFA). Thus, we hypothesized that the 10,12 CLA\u2010mediated decrease in SCD\u20101, with the subsequent decrease in MUFA, was responsible for the observed effects. To test this hypothesis, 10,12 CLA\u2010treated human adipocytes were supplemented with oleic acid for 12 h to 7 days, and inflammatory gene expression, insulin\u2010stimulated glucose uptake, and lipid content were measured. Oleic acid reduced inflammatory gene expression in a dose\u2010dependent manner, and restored the lipid content of 10,12 CLA\u2010treated adipocytes without improving insulin\u2010stimulated glucose uptake. In contrast, supplementation with stearic acid, a substrate for SCD\u20101, or 9,11 CLA did not prevent inflammatory gene expression by 10,12 CLA. Notably, 10,12 CLA impacted the expression of several G\u2010protein coupled receptors that was attenuated by oleic acid. Collectively, these data show that oleic acid attenuates 10,12 CLA\u2010induced inflammatory gene expression and lipid content, possibly by alleviating cell stress caused by the inhibition of MUFA needed for phospholipid and neutral lipid synthesis.<\/jats:p>","DOI":"10.1007\/s11745-012-3711-0","type":"journal-article","created":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T16:45:37Z","timestamp":1346517937000},"page":"1043-1051","source":"Crossref","is-referenced-by-count":25,"title":["Oleic Acid Attenuates <i>trans<\/i>\u201010,<i>cis<\/i>\u201012 Conjugated Linoleic Acid\u2010Mediated Inflammatory Gene Expression in Human Adipocytes"],"prefix":"10.1002","volume":"47","author":[{"given":"Meaghan","family":"Reardon","sequence":"first","affiliation":[{"name":"Department of Nutrition University of North Carolina at Greensboro PO Box 26170 318 Stone Building Greensboro NC 27402\u20106170 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