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Two effects were here investigated: that of CLA on inflammatory mediator production in human lung cancer cells, and that of reduced mediators on the myogenic differentiation of murine muscle C2C12 cells. The latter cells were grown in medium conditioned by human lung cancer A427 cells, with or without CLA, to mimic only the effect of molecules released from the tumor \u201cin vivo\u201d, excluding the effect of host\u2010produced cachectic factors. The results obtained show that CLA was found to reduce the production of tumor necrosis factor\u2010\u03b1, interleukin (IL)\u20101\u03b2 and prostaglandin E2 (PGE2), but had no effect on IL\u20106 production. The mechanisms underlying the effect of CLA on cytokine or PGE2 release in A427 cells are probably mediated by activation of peroxisome proliferator\u2010activated receptor (PPAR)\u03b1, which increased at 24 h CLA treatment. In turn, the reduced content of inflammatory mediators in medium conditioned by A427 cells, in the presence of CLA, allowed muscle cells to proliferate, again by inducing PPAR. The involvement of PPAR\u03b1 was demonstrated by treatment with the antagonist MK\u2010886. The findings demonstrate the anti\u2010inflammatory and myogenic action of CLA and point to its possible application as a novel dietary supplement and therapeutic agent in inflammatory disease states, such as cachexia.<\/jats:p>","DOI":"10.1007\/s11745-012-3734-6","type":"journal-article","created":{"date-parts":[[2012,11,5]],"date-time":"2012-11-05T21:13:37Z","timestamp":1352150017000},"page":"29-38","source":"Crossref","is-referenced-by-count":15,"title":["CLA Reduces Inflammatory Mediators from A427 Human Lung Cancer Cells and A427 Conditioned Medium Promotes Differentiation of C2C12 Murine Muscle Cells"],"prefix":"10.1002","volume":"48","author":[{"given":"Manuela","family":"Oraldi","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina ed Oncologia Sperimentale Universit\u00e0 di Torino Corso Raffello 30 Turin 10125 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