{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:12:20Z","timestamp":1792660340918,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,26]],"date-time":"2013-01-26T00:00:00Z","timestamp":1359158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have developed a protocol suitable for high\u2010throughput lipidomic analysis of human brain samples. The traditional Folch extraction (using chloroform and glass\u2013glass homogenization) was compared to a high\u2010throughput method combining methyl\u2010<jats:italic>tert<\/jats:italic>\u2010butyl ether (MTBE) extraction with mechanical homogenization utilizing ceramic beads. This high\u2010throughput method significantly reduced sample handling time and increased efficiency compared to glass\u2013glass homogenizing. Furthermore, replacing chloroform with MTBE is safer (less carcinogenic\/toxic), with lipids dissolving in the upper phase, allowing for easier pipetting and the potential for automation (i.e., robotics). Both methods were applied to the analysis of human occipital cortex. Lipid species (including ceramides, sphingomyelins, choline glycerophospholipids, ethanolamine glycerophospholipids and phosphatidylserines) were analyzed via electrospray ionization mass spectrometry and sterol species were analyzed using gas chromatography mass spectrometry. No differences in lipid species composition were evident when the lipid extraction protocols were compared, indicating that MTBE extraction with mechanical bead homogenization provides an improved method for the lipidomic profiling of human brain tissue.<\/jats:p>","DOI":"10.1007\/s11745-013-3760-z","type":"journal-article","created":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T02:24:07Z","timestamp":1359080647000},"page":"307-318","source":"Crossref","is-referenced-by-count":80,"title":["An Improved High\u2010Throughput Lipid Extraction Method for the Analysis of Human Brain Lipids"],"prefix":"10.1002","volume":"48","author":[{"given":"Sarah K.","family":"Abbott","sequence":"first","affiliation":[{"name":"Illawarra Health and Medical Research Institute University of Wollongong Wollongong NSW 2522 Australia"},{"name":"School of Biological Sciences University of Wollongong Wollongong NSW 2522 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Jenner","sequence":"additional","affiliation":[{"name":"Illawarra Health and Medical Research Institute University of Wollongong Wollongong NSW 2522 Australia"},{"name":"School of Biological Sciences University of Wollongong Wollongong NSW 2522 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd W.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Illawarra Health and Medical Research Institute University of Wollongong Wollongong NSW 2522 Australia"},{"name":"School of Health Sciences University of Wollongong Wollongong NSW 2522 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon H. J.","family":"Brown","sequence":"additional","affiliation":[{"name":"Illawarra Health and Medical Research Institute University of Wollongong Wollongong NSW 2522 Australia"},{"name":"School of Health Sciences University of Wollongong Wollongong NSW 2522 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda M.","family":"Halliday","sequence":"additional","affiliation":[{"name":"Neuroscience Research Australia and the University of New South Wales Sydney NSW 2031 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Garner","sequence":"additional","affiliation":[{"name":"Illawarra Health and Medical Research Institute University of Wollongong Wollongong NSW 2522 Australia"},{"name":"School of Biological Sciences University of Wollongong Wollongong NSW 2522 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2011.11.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197402000-00004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017299"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2011.00119"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110566"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2010.06.003"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2011.132"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr800188y"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/475S20a"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385883-2.00008-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2006\/202938"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anchem.111808.073705"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf073471e"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(85)90277-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2007.12.001"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-009-0087-5"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-011-0293-4"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.10.002"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D700041-JLR200"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4273-8"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2012.04.019"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006261"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-011-3531-7"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.2031"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2008.04.002"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-4609"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac060545x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2004.07.009"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2007.09.004"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00931-F"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.00997.x"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s11745-013-3760-z","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:00:36Z","timestamp":1760950836000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/s11745-013-3760-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,26]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1007\/s11745-013-3760-z"],"URL":"https:\/\/doi.org\/10.1007\/s11745-013-3760-z","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"value":"0024-4201","type":"print"},{"value":"1558-9307","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,26]]}}}