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The aim of this study was to evaluate dried blood long chain polyunsaturated fatty acids (LC\u2010PUFA) stability during storage at \u221228 \u00b0C. We collected 12 blood cord samples that were analyzed immediately after blood drawing, with and without drying the blood on filter paper. Dried samples were then analyzed 7 days and 1, 3, and 6 months after collection. Butylated hydroxytoluene was added to all samples. Fatty acid composition and <jats:sup>13<\/jats:sup>C enrichment were measured by gas chromatography and by gas chromatography\u2010isotope ratio mass spectrometry, respectively. The fatty acid composition, expressed in mol%, of the major LC\u2010PUFA at day 7 was not statistically different from time 0, however lower values were found by the first month of storage. The <jats:sup>13<\/jats:sup>C enrichment of 20:4n\u20106 and 22:6n\u20103 did not differ during the whole study period. LC\u2010PUFA analysis from dried umbilical cord blood in neonates should be performed within a week, major losses of LC\u2010PUFA occur afterwards. However, fatty acids obtained from dried blood maintain their <jats:sup>13<\/jats:sup>C enrichment value for up to 6 months and thus these samples are suitable for natural abundance isotopic studies.<\/jats:p>","DOI":"10.1007\/s11745-015-4111-z","type":"journal-article","created":{"date-parts":[[2016,1,9]],"date-time":"2016-01-09T00:14:16Z","timestamp":1452298456000},"page":"193-198","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Short\u2010Term Stability of Whole Blood Polyunsaturated Fatty Acid Content on Filter Paper During Storage at \u221228 \u00b0C"],"prefix":"10.1002","volume":"51","author":[{"given":"Daniele","family":"Pupillo","sequence":"first","affiliation":[{"name":"Division of Neonatology Polytechnic University of Marche and Salesi's Children Hospital Via Corridoni 11 Ancona 62123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Simonato","sequence":"additional","affiliation":[{"name":"Pediatric Research Institute \u201cCitt\u00e0 della Speranza\u201d Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola E.","family":"Cogo","sequence":"additional","affiliation":[{"name":"Division of Pediatrics Department of Clinical and Experimental Medical Sciences, University of Udine Udine 33100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Lapillonne","sequence":"additional","affiliation":[{"name":"Department of Neonatology, APHP Necker Hospital Paris Descartes University Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virgilio P.","family":"Carnielli","sequence":"additional","affiliation":[{"name":"Division of Neonatology Polytechnic University of Marche and Salesi's Children Hospital Via Corridoni 11 Ancona 62123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02562220"},{"issue":"1","key":"e_1_2_7_3_1","first-page":"S51","article-title":"Fats for brains","volume":"46","author":"Koletzko B","year":"1992","journal-title":"Eur J Clin Nutr"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.4.855"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/86.5.1323"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200012000-00022"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3016.2010.01105.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2010.03.002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2008.945"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-999-0371-y"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1999.tb02453.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.8.2319"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2003.12.016"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2014.09.009"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-007-3140-7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511001978"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2010.10.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2007.05.003"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-014-3918-3"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0009922807311730"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-002-0927-x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-7037(57)90024-8"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-8-35"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-014-0628-z"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2009.05.018"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-013-3827-x"},{"key":"e_1_2_7_27_1","unstructured":"Centre for Disease Control and Prevention(2009)Filter paper comparison study.http:\/\/www.cdc.gov\/labstandards\/pdf\/nsqap\/nsqap_FilterPaperStudy51809.pdf. 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