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However, conservation of gametes is extremely important, particularly in the context of preserving endangered species. As spermatozoa are comprised of different membrane systems, the composition of these membranes might account for difficulties in cryopreservation. Lipids, as the main components, affect the physical properties of biological membranes and play a major role in sperm maturation. Therefore, knowledge of the lipid composition is crucial for any further step toward the preservation of the species. We used MALDI\u2010TOF, ESI\u2010IT, tandem mass spectrometry, and thin layer chromatography to investigate the lipid composition of epididymal spermatozoa of four different kangaroo species. Spectra of these species were very similar with respect to the identified lipid species. Tremendous changes in the lipid composition during the transit of sperm from caput to cauda epididymis could be seen, specifically an increase in poly\u2010unsaturated fatty acids, ether lipids, and plasmalogens, as well as a reduction in mono\u2010 and di\u2010unsaturated fatty acids. Additionally, phosphatidylcholines containing docosatrienoic acid (22:3), a heretofore unknown fatty acid for sperm membranes, showed the highest abundance in kangaroo sperm.<\/jats:p>","DOI":"10.1007\/s11745-017-4283-9","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T04:25:23Z","timestamp":1502425523000},"page":"857-869","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Phospholipid Composition of Kangaroo Spermatozoa Verified by Mass Spectrometric Lipid Analysis"],"prefix":"10.1002","volume":"52","author":[{"given":"Kathrin M.","family":"Engel","sequence":"first","affiliation":[{"name":"Institute of Medical Physics and Biophysics, Faculty of Medicine University of Leipzig H\u00e4rtelstr. 16\/18 Leipzig 04107 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