{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:41:31Z","timestamp":1760967691419,"version":"build-2065373602"},"reference-count":12,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[1998,8,1]],"date-time":"1998-08-01T00:00:00Z","timestamp":901929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1998,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heating or freezing leads to loss in infectivity of oocysts of <jats:italic>Cryptosporidium parvum<\/jats:italic> toward neonatal BALB\/c mice and is reflected in the profile of the polar lipid fatty acids. Upon loss of infectivity, the ratio of polar lipid to neutral lipid fatty acid decreased and the relative proportions of 18\u22361n\u20109 also decreased; proportions of 18\u22362n\u20106 and 20\u22365n\u20106 increased, whereas the proportions of 16\u22360 remained constant with freezing. During these investigations, a novel fatty acid, 10\u2010OH 18\u22360, was discovered in the glycolipid fraction. The identification of a fatty acid unique to species of <jats:italic>Cryptosporidium<\/jats:italic> was thought to provide a specific biomarker for this organism. <jats:italic>Cryptosporidium<\/jats:italic> also demonstrated fluctuations in absolute quantities of 10\u2010OH 18\u22360 with events that lead to loss of infectivity. This led to the presumed correlation of this biomarker with infectious <jats:italic>Cryptosporidium<\/jats:italic>. The 10\u2010OH 18\u22360 was putatively localized at the <jats:italic>sn<\/jats:italic>\u20102 position of phosphatidylethanolamine. High\u2010performance liquid chromatography\/electrospray ionization mass spectrometry revealed that the 10\u2010OH 18\u22360 existed principally in the free fatty acid form. Herein, we establish that the free fatty acid 10\u2010OH 18\u22360 was, in actuality, an artifact of the procedures for sample preparation.<\/jats:p>","DOI":"10.1007\/s11745-998-0278-7","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T13:31:33Z","timestamp":1163424693000},"page":"829-833","source":"Crossref","is-referenced-by-count":0,"title":["The signature 10\u2010hydroxy stearic acid thought to correlate with infectivity in oocysts of <i>Cryptosporidium<\/i> species is an artifact"],"prefix":"10.1002","volume":"33","author":[{"given":"Robert S.","family":"Burkhalter","sequence":"first","affiliation":[{"name":"Center for Environmental Biotechnology University of Iennessee 10515 Research Dr., Suite 300 Knoxville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology University of Iennessee 10515 Research Dr., Suite 300 Knoxville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C.","family":"White","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology University of Iennessee 10515 Research Dr., Suite 300 Knoxville Tennessee"},{"name":"Environmental Science Division Oak Ridge National Laboratory Oak Ridge Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"Fayer","sequence":"additional","affiliation":[{"name":"Livestock and Poultry Sciences Institute, Parasite Immunology Laboratory USDA, ARS Beltsville Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew B.","family":"White","sequence":"additional","affiliation":[{"name":"Microbial Insights, Inc. Rockford Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,8]]},"reference":[{"volume-title":"Cryptosporidium and Cryptosporidiosis","year":"1997","author":"Fayer R.","key":"e_1_2_2_2_1"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00119a727"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.62.4.1431-1433.1996"},{"key":"e_1_2_2_5_1","first-page":"53","volume-title":"Proceedings","author":"White D.C.","year":"1997"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-997-0102-4"},{"key":"e_1_2_2_7_1","unstructured":"Burkhalter R.S. Smith C.A. Peacock A. White D.C. Whitaker K.W. andFayer R.(1998).Detection of Single InfectiousCryptosporidiumOocyst by Signature Free Fatty Acid 10\u2010OH 18:0 Abstracts Annual meeting of the American Society for Microbiology Atlanta May 1998."},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00388810"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1985.tb01143.x"},{"volume-title":"Detection of Signature Lipid Biomarkers Specific to Infectious Cryptosporidium","year":"1998","author":"Burkhalter R.S.","key":"e_1_2_2_11_1"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00125a017"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3545646"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s11745-998-0278-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:08:24Z","timestamp":1760965704000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/s11745-998-0278-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,8]]},"references-count":12,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1998,8]]}},"alternative-id":["10.1007\/s11745-998-0278-7"],"URL":"https:\/\/doi.org\/10.1007\/s11745-998-0278-7","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"type":"print","value":"0024-4201"},{"type":"electronic","value":"1558-9307"}],"subject":[],"published":{"date-parts":[[1998,8]]}}}