{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:40:19Z","timestamp":1761007219639,"version":"build-2065373602"},"reference-count":60,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1999,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study further elucidates the involvement of the \u03b1\u2010subunit of the GTP\u2010binding protein G<jats:sub>i2<\/jats:sub> in the differentiation of murine 313\u2010L1 cells. Control and vector\u2010transfected cells attained a fully differentiated adipocyte phenotype showing ample lipid droplets. Cells expressing wild type (WT)\u2010G<jats:sub>i2<\/jats:sub>\u03b1 or the constitutively active R179E\u2010G<jats:sub>i2<\/jats:sub>\u03b1, however, became enlarged, less confluent, and produced large amounts of lipids. Differentiation consistently increased the triglyceride (TAG) content in control cells. In both WT\u2010G<jats:sub>i2<\/jats:sub>\u03b1 and R179E\u2010G<jats:sub>i2<\/jats:sub>\u03b1 clones, a marked increase in TAG could be detected even prior to insulin\/dexamethasone\/isobutyl methylxanthine exposure. The activity of palmitoyl\u2010CoA synthetase (PCS) and glycerophosphate acyltransferase (GPAT) also increased upon differentiation. WT\u2010G<jats:sub>i2<\/jats:sub>\u03b1 and R179E\u2010G<jats:sub>i2<\/jats:sub>\u03b1 overexpression also enhanced PCS and GPAT activities even before differentiation medium was added. The total amount of phospholipids (PL) generally increased upon differentiation; however, pre\u2010 and postdifferentiation values were insignificantly different in cells expressing WT\u2010G<jats:sub>i2<\/jats:sub>\u03b1 and R179E\u2010G<jats:sub>i2<\/jats:sub>\u03b1. Differentiation altered the PL profile with a relative shift from phosphatidylcholine and phosphatidylethanolamine to phosphatidylinositol (PI) in differentiated cells. Finally, differentiation yielded a general increase in the activity of basal PI\u2010phospholipase\u2010C activity. Again, cells expressing WT\u2010G<jats:sub>i2<\/jats:sub>\u03b1 and R179E\u2010G<jats:sub>i2<\/jats:sub>\u03b1 demonstrated elevated enzyme activity and enhanced second messenger accumulation subsequent to differentiation. In summary, cells with the R179E\u2010mutants of G<jats:sub>i2<\/jats:sub>\u03b1 exhibited stimulated lipid turnover and accumulation in both undifferentiated and differentiated cells.<\/jats:p>","DOI":"10.1007\/s11745-999-0373-9","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T14:01:24Z","timestamp":1163426484000},"page":"355-362","source":"Crossref","is-referenced-by-count":2,"title":["The inhibitory guanine nucleotide\u2010binding protein G<sub>i2<\/sub>\u03b1 induces and potentiates adipocyte differentiation"],"prefix":"10.1002","volume":"34","author":[{"given":"Kjerstin E.","family":"H\u00f8vik","sequence":"first","affiliation":[{"name":"Institute of Medical Biochemistry University of Oslo P.O. Box 1112, Blindern Oslo 0317 Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Medical Biochemistry University of Oslo P.O. Box 1112, Blindern Oslo 0317 Norway"},{"name":"Department of Biochemistry Xinjiang Medical College Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan O.","family":"Gordeladze","sequence":"additional","affiliation":[{"name":"Institute of Medical Biochemistry University of Oslo P.O. 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