{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:11:38Z","timestamp":1759104698286},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2008,7,25]],"date-time":"2008-07-25T00:00:00Z","timestamp":1216944000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2009,6]]},"DOI":"10.1007\/s12010-008-8309-x","type":"journal-article","created":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T13:11:11Z","timestamp":1216905071000},"page":"554-561","source":"Crossref","is-referenced-by-count":1,"title":["Functional Expression of Bacillus anthracis Protective Antigen in E. coli"],"prefix":"10.1007","volume":"157","author":[{"given":"Fatemeh","family":"Vahedi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojtaba","family":"Sankian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Mahmoudi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,7,25]]},"reference":[{"key":"8309_CR1","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.2105\/AJPH.2006.102749","volume":"97","author":"M. M. Weiss","year":"2007","unstructured":"Weiss, M. M., Weiss, P. D., & Weiss, J. B. (2007). American Journal of Public Health, 97, 1945\u20131951. doi: 10.2105\/AJPH.2006.102749 .","journal-title":"American Journal of Public Health"},{"key":"8309_CR2","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1038\/385833a0","volume":"385","author":"C. Petosa","year":"1997","unstructured":"Petosa, C., et al. (1997). Nature, 385, 833\u2013838. doi: 10.1038\/385833a0 .","journal-title":"Nature"},{"key":"8309_CR3","first-page":"134","volume":"3","author":"F. Vahedi","year":"2005","unstructured":"Vahedi, F., et al. (2005). Iranian Journal of Immunology, 3, 134\u2013140.","journal-title":"Iranian Journal of Immunology"},{"key":"8309_CR4","first-page":"437","volume":"192","author":"F. Brossier","year":"1998","unstructured":"Brossier, F., Guidi-Rontani, C., & Mock, M. (1998). Comptes Rendus des Seances de la Soci\u00e9t\u00e9 de Biologie et de ses Filiales, 192, 437\u2013444.","journal-title":"Comptes Rendus des Seances de la Soci\u00e9t\u00e9 de Biologie et de ses Filiales"},{"key":"8309_CR5","doi-asserted-by":"crossref","first-page":"13803","DOI":"10.1073\/pnas.2436299100","volume":"100","author":"M. Mourez","year":"2003","unstructured":"Mourez, M., et al. (2003). Proceedings of the National Academy of Sciences of the United States of America, 100, 13803\u201313808. doi: 10.1073\/pnas.2436299100 .","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"8309_CR6","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1111\/j.1365-2672.2006.03112.x","volume":"101","author":"L. W. Baillie","year":"2006","unstructured":"Baillie, L. W. (2006). Journal of Applied Microbiology, 101, 594\u2013606. doi: 10.1111\/j.1365-2672.2006.03112.x .","journal-title":"Journal of Applied Microbiology"},{"key":"8309_CR7","doi-asserted-by":"crossref","first-page":"143","DOI":"10.5144\/0256-4947.2005.143","volume":"25","author":"M. Splino","year":"2005","unstructured":"Splino, M., et al. (2005). Annals of Saudi Medicine, 25, 143\u2013149.","journal-title":"Annals of Saudi Medicine"},{"key":"8309_CR8","doi-asserted-by":"crossref","first-page":"3679","DOI":"10.1016\/j.vaccine.2007.01.056","volume":"25","author":"R. Grunow","year":"2007","unstructured":"Grunow, R., et al. (2007). Vaccine, 25, 3679\u20133683. doi: 10.1016\/j.vaccine.2007.01.056 .","journal-title":"Vaccine"},{"key":"8309_CR9","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.femsim.2005.05.008","volume":"45","author":"G. E. Rhie","year":"2005","unstructured":"Rhie, G. E., et al. (2005). FEMS Immunology and Medical Microbiology, 45, 331\u2013339. doi: 10.1016\/j.femsim.2005.05.008 .","journal-title":"FEMS Immunology and Medical Microbiology"},{"key":"8309_CR10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1472-765X.1994.tb00949.x","volume":"19","author":"L. W. Baillie","year":"1994","unstructured":"Baillie, L. W., Johnson, M., & Manchee, R. J. (1994). Letters in Applied Microbiology, 19, 225\u2013227. doi: 10.1111\/j.1472-765X.1994.tb00949.x .","journal-title":"Letters in Applied Microbiology"},{"key":"8309_CR11","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1006\/bbrc.2001.4777","volume":"283","author":"V. Chauhan","year":"2001","unstructured":"Chauhan, V., et al. (2001). Biochemical and Biophysical Research Communications, 283, 308\u2013315. doi: 10.1006\/bbrc.2001.4777 .","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"8309_CR12","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1006\/bbrc.2001.5282","volume":"285","author":"P. Gupta","year":"2001","unstructured":"Gupta, P., Sahai, V., & Bhatnagar, R. (2001). Biochemical and Biophysical Research Communications, 285, 1025\u20131033. doi: 10.1006\/bbrc.2001.5282 .","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"8309_CR13","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.pep.2005.04.009","volume":"45","author":"W. Gwinn","year":"2006","unstructured":"Gwinn, W., et al. (2006). Protein Expression and Purification, 45, 30\u201336. doi: 10.1016\/j.pep.2005.04.009 .","journal-title":"Protein Expression and Purification"},{"key":"8309_CR14","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1016\/j.vaccine.2005.10.018","volume":"24","author":"R. W. Hepler","year":"2006","unstructured":"Hepler, R. W., et al. (2006). Vaccine, 24, 1501\u20131514. doi: 10.1016\/j.vaccine.2005.10.018 .","journal-title":"Vaccine"},{"key":"8309_CR15","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"U. K. Laemmli","year":"1970","unstructured":"Laemmli, U. K. (1970). Nature, 227, 680\u2013685. doi: 10.1038\/227680a0 .","journal-title":"Nature"},{"key":"8309_CR16","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"M. Bradford","year":"1976","unstructured":"Bradford, M. (1976). Analytical Biochemistry, 72, 248\u2013254. doi: 10.1016\/0003-2697(76)90527-3 .","journal-title":"Analytical Biochemistry"},{"key":"8309_CR17","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/jmbi.1996.0399","volume":"260","author":"B. Miroux","year":"1996","unstructured":"Miroux, B., & Walker, J. E. (1996). Journal of Molecular Biology, 260, 289\u2013298. doi: 10.1006\/jmbi.1996.0399 .","journal-title":"Journal of Molecular Biology"},{"key":"8309_CR18","doi-asserted-by":"crossref","first-page":"17186","DOI":"10.1016\/S0021-9258(18)41911-4","volume":"267","author":"J. M. Novak","year":"1992","unstructured":"Novak, J. M., et al. (1992). The Journal of Biological Chemistry, 267, 17186\u201317193.","journal-title":"The Journal of Biological Chemistry"},{"key":"8309_CR19","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1006\/abbi.1995.1002","volume":"316","author":"T. C. Friedman","year":"1995","unstructured":"Friedman, T. C., et al. (1995). Archives of Biochemistry and Biophysics, 316, 5\u201313. doi: 10.1006\/abbi.1995.1002 .","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"8309_CR20","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/0092-8674(83)90402-6","volume":"34","author":"M. H. Vodkin","year":"1983","unstructured":"Vodkin, M. H., & Leppla, S. H. (1983). Cell, 34, 693\u2013697. doi: 10.1016\/0092-8674(83)90402-6 .","journal-title":"Cell"},{"key":"8309_CR21","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1006\/prep.1996.0005","volume":"7","author":"M. Sharma","year":"1996","unstructured":"Sharma, M., et al. (1996). Protein Expression and Purification, 7, 33\u201338. doi: 10.1006\/prep.1996.0005 .","journal-title":"Protein Expression and Purification"},{"key":"8309_CR22","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1006\/bbrc.2001.5337","volume":"286","author":"N. Ahuja","year":"2001","unstructured":"Ahuja, N., Kumar, P., & Bhatnagar, R. (2001). Biochemical and Biophysical Research Communications, 286, 6\u201311. doi: 10.1006\/bbrc.2001.5337 .","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"8309_CR23","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1006\/prep.1999.1066","volume":"16","author":"P. Gupta","year":"1999","unstructured":"Gupta, P., Waheed, S. M., & Bhatnagar, R. (1999). Protein Expression and Purification, 16, 369\u2013376. doi: 10.1006\/prep.1999.1066 .","journal-title":"Protein Expression and Purification"},{"key":"8309_CR24","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1042\/bj2520753","volume":"252","author":"C. P. Quinn","year":"1988","unstructured":"Quinn, C. P., et al. (1988). The Biochemical Journal, 252, 753\u2013758.","journal-title":"The Biochemical Journal"},{"key":"8309_CR25","first-page":"105","volume":"13","author":"P. Hambleton","year":"1990","unstructured":"Hambleton, P., & Turnbull, P. C. (1990). Advances in Biotechnological Processes, 13, 105\u2013122.","journal-title":"Advances in Biotechnological Processes"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-008-8309-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12010-008-8309-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-008-8309-x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T19:54:29Z","timestamp":1631562869000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12010-008-8309-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,25]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["8309"],"URL":"https:\/\/doi.org\/10.1007\/s12010-008-8309-x","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,25]]}}}