{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:49:37Z","timestamp":1790394577127,"version":"4.1.0"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"5-6","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2011,11]]},"DOI":"10.1007\/s12010-011-9348-2","type":"journal-article","created":{"date-parts":[[2011,8,31]],"date-time":"2011-08-31T10:47:32Z","timestamp":1314787652000},"page":"1308-1321","source":"Crossref","is-referenced-by-count":26,"title":["New Urea Biosensor Based on Urease Enzyme Obtained from Helycobacter pylori"],"prefix":"10.1007","volume":"165","author":[{"given":"Bahar","family":"Dindar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emine","family":"Karaku\u015f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih","family":"Abas\u0131yan\u0131k","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,9,1]]},"reference":[{"issue":"16","key":"9348_CR1","doi-asserted-by":"crossref","first-page":"9464","DOI":"10.1016\/S0021-9258(19)38872-6","volume":"265","author":"BE Dunn","year":"1990","unstructured":"Dunn, B. E., Campbell, G. P., Perez-Perez, G. I., & Blaser, M. J. (1990). Purification and characterization of urease from Helicobacter pylori. The Journal of Biological Chemistry, 265(16), 9464\u20139469.","journal-title":"The Journal of Biological Chemistry"},{"issue":"8","key":"9348_CR2","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1128\/JB.181.8.2477-2484.1999","volume":"181","author":"DJ McGee","year":"1999","unstructured":"McGee, D. J., May, C. A., Garner, R. M., Himpsl, J. M., & Mobley, H. L. T. (1999). Isolation of Helicobacter pylori genes that modulate urease activity. Journal of Bacteriology, 181(8), 2477\u20132484.","journal-title":"Journal of Bacteriology"},{"key":"9348_CR3","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1081\/BIO-200066632","volume":"33","author":"E Karakus","year":"2005","unstructured":"Karakus, E., Pekyard\u0131mci, S., & K\u0131l\u0131c, E. (2005). Urea biosensor based on PVC membrane containing palmitic acid. Artificial Cells Blood Substitutes and Biotechnology, 33, 329.","journal-title":"Artificial Cells Blood Substitutes and Biotechnology"},{"key":"9348_CR4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0956-5663(98)00103-1","volume":"14","author":"C Eggenstein","year":"1999","unstructured":"Eggenstein, C., Borchardt, M., Diekmann, C., Grundig, B., Dumschat, C., Cammann, K., et al. (1999). A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer. Biosensors and Bioelectronics, 14, 33.","journal-title":"Biosensors and Bioelectronics"},{"key":"9348_CR5","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0003-2670(95)00046-3","volume":"307","author":"D Liu","year":"1995","unstructured":"Liu, D., Ge, K., Cheng, K., Nie, L., & Yao, S. (1995). Clinical analysis of urea in human blood by coupling a surface acoustic wave sensor with urease extracted from pumpkin seeds. Analytical Chimica Acta, 307, 61.","journal-title":"Analytical Chimica Acta"},{"key":"9348_CR6","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/0039-9140(91)80243-S","volume":"38","author":"X Xie","year":"1991","unstructured":"Xie, X., Suleiman, A. A., & Guilbault, G. G. (1991). Determination of urea in serum by a fiber-optic fluorescence biosensor. Talanta, 38, 1197.","journal-title":"Talanta"},{"key":"9348_CR7","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/0003-2670(93)85022-C","volume":"281","author":"SB Adeloju","year":"1993","unstructured":"Adeloju, S. B., Shaw, S. J., & Wallace, G. G. (1993). Polypyrrole-based potentiometric biosensor for urea part 1. Incorporation of urease. Analytical Chimica Acta, 281, 611.","journal-title":"Analytical Chimica Acta"},{"key":"9348_CR8","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0003-2670(93)80602-H","volume":"274","author":"D Liu","year":"1993","unstructured":"Liu, D., Meyerhoff, M. E., Goldberg, H. D., & Brown, R. B. (1993). Potentiometric ion and bioselective electrodes based on asymmetric polyurethane membranes. Analytical Chimica Acta, 274, 37.","journal-title":"Analytical Chimica Acta"},{"key":"9348_CR9","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1016\/0039-9140(94)00197-9","volume":"41","author":"K Chen","year":"1994","unstructured":"Chen, K., Liu, D., Nie, L., & Yao, S. (1994). Determination of urea in urine using a conductivity cell with surface acoustic wave resonator-based measurement circuit. Talanta, 41, 2195.","journal-title":"Talanta"},{"key":"9348_CR10","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1021\/ac950655w","volume":"68","author":"H Sangodkar","year":"1996","unstructured":"Sangodkar, H., Sukeerthi, S., Srinivasa, R. S., Lal, R., & Contractor, A. Q. A. (1996). Biosensor array based on polyaniline. Analytical Chemistry, 68, 779.","journal-title":"Analytical Chemistry"},{"key":"9348_CR11","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1021\/ac50029a030","volume":"50","author":"RE Adams","year":"1978","unstructured":"Adams, R. E., & Carr, P. W. (1978). Coulometric flow analyzer for use with immobilized enzyme reactors. Analytical Chemistry, 50, 944.","journal-title":"Analytical Chemistry"},{"key":"9348_CR12","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0003-2670(95)00562-5","volume":"323","author":"SB Adeloju","year":"1996","unstructured":"Adeloju, S. B., Shaw, S. J., & Wallace, G. G. (1996). Polypyrrole-based amperometric flow injection biosensor for urea. Analytica Chimica Acta, 323, 107.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR13","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1021\/ac981367d","volume":"71","author":"WO Ho","year":"1999","unstructured":"Ho, W. O., Krause, S., McNeil, C. J., Pritchard, J. A., Armstrong, R. D., Athey, D., et al. (1999). Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation. Analytical Chemistry, 71, 1940.","journal-title":"Analytical Chemistry"},{"key":"9348_CR14","doi-asserted-by":"crossref","unstructured":"Gupta, B., Singh, S., Mohan, S., & Prakash, R. (2010). Urea Biosensor Based on Conducting Polymer Transducers. Biosensors, Book edited by: Pier Andrea Serra, February 2010, INTECH, Croatia. pp. 302","DOI":"10.5772\/7205"},{"key":"9348_CR15","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/978-3-642-84038-8","volume-title":"Klinishe Chemie und Mikroskopie","author":"W Rick","year":"1990","unstructured":"Rick, W. (1990). Klinishe Chemie und Mikroskopie (pp. 245\u2013247). Berlin: Springer."},{"key":"9348_CR16","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1021\/ac50038a011","volume":"51","author":"J Ruzicka","year":"1979","unstructured":"Ruzicka, J., Hansen, E. H., Ghose, A. K., & Mottola, H. A. (1979). Enzyme determination of urea in serum based on pH measurement with the flow injection method. Analytical Chemistry, 51, 199\u2013203.","journal-title":"Analytical Chemistry"},{"key":"9348_CR17","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0003-2670(01)95405-4","volume":"136","author":"PM Vadgama","year":"1982","unstructured":"Vadgama, P. M., Alberti, K. G. M. M., & Covington, A. K. (1982). Determination of urea in blood plasma by enzyme-pH electrode. Analytica Chimica Acta, 136, 403\u2013406.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0250-6874(85)87001-3","volume":"7","author":"Y Miyahara","year":"1985","unstructured":"Miyahara, Y., Moriizumi, T., & Ichimura, K. (1985). Integrated enzyme FETs for simultaneous determination of urea and glucose. Sensors and Actuators, 7, 1\u201310.","journal-title":"Sensors and Actuators"},{"key":"9348_CR19","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0003-2670(86)80046-0","volume":"185","author":"I Karube","year":"1986","unstructured":"Karube, I., Tamiya, E., & Dicks, J. M. (1986). A microsensor for urea based on an ion-selective field effect transistor. Analytica Chimica Acta, 185, 195\u2013200.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR20","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0022-0728(86)80186-3","volume":"209","author":"H Hamann","year":"1986","unstructured":"Hamann, H., K\u00fchn, M., B\u00f6ttcher, N., & Scheller, F. (1986). Enzyme sensor system for potentiometric urea determination in serum and milk. Journal of Electroanalytical Chemistry, 209, 69\u201379.","journal-title":"Journal of Electroanalytical Chemistry"},{"key":"9348_CR21","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1021\/ja00711a052","volume":"92","author":"GG Guilbault","year":"1970","unstructured":"Guilbault, G. G., & Montalvo, J. G. (1970). An enzyme electrode for the substrate urea. Journal of the American Chemical Society, 92, 2533\u20132538.","journal-title":"Journal of the American Chemical Society"},{"key":"9348_CR22","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0302-4598(90)85008-6","volume":"23","author":"L Campanella","year":"1990","unstructured":"Campanella, L., Mazzei, F., Sammartino, M. P., & Tomassetti, M. (1990). New enzyme sensors for urea and creatinine analysis. Bioelectrochemistry and Bioenergetics, 23, 195\u2013202.","journal-title":"Bioelectrochemistry and Bioenergetics"},{"issue":"11","key":"9348_CR23","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1080\/00032719608000800","volume":"29","author":"I Walcerz","year":"1996","unstructured":"Walcerz, I., Koncki, R., Leszczynska, E., Salamonowicz, B., & Glab, S. (1996). Enzyme biosensors for urea determination based on an ionophore free membrane electrode. Analytical Letters, 29(11), 1939\u20131953.","journal-title":"Analytical Letters"},{"key":"9348_CR24","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0003-2670(96)00266-8","volume":"333","author":"R Koncki","year":"1996","unstructured":"Koncki, R., Walcerz, I., Ruckruh, F., & Glab, S. (1996). Bienzymatic potentiometric electrodes for creatine and L-arginine determination. Analytica Chimica Acta, 333, 215\u2013222.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR25","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0003-2670(96)00031-1","volume":"325","author":"J Schneider","year":"1996","unstructured":"Schneider, J., Gr\u00fcndig, B., Renneberg, R., Cammann, K., Madaras, M. B., Buck, R. P., et al. (1996). Hydrogel matrix for three enzyme entrapment in creatine\/creatinine amperometric biosensing. Analytica Chimica Acta, 325, 161\u2013167.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR26","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.bioelechem.2004.03.004","volume":"64","author":"J Jasnowska","year":"2004","unstructured":"Jasnowska, J., Ligaj, M., Stupnicka, B., & Filipiak, M. (2004). DNA sensor for o-dianisidine. Bioelectrochemistry, 64, 85\u201390.","journal-title":"Bioelectrochemistry"},{"key":"9348_CR27","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0003-2697(87)90671-3","volume":"161","author":"JI Korenbrot","year":"1987","unstructured":"Korenbrot, J. I., Perry, R., & Copenhagen, D. R. (1987). Development and characterization of a polymer gel with an immobilized enzyme to measure l-glutamate. Analytical Biochemistry, 161, 187\u2013199.","journal-title":"Analytical Biochemistry"},{"key":"9348_CR28","unstructured":"Berthelot, MPE (1859). Berthelot\u2019s reaction mechanism. Report Chim. Appl. 2884."},{"key":"9348_CR29","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0039-9140(98)00066-6","volume":"47","author":"JMCS Magalhaes","year":"1998","unstructured":"Magalhaes, J. M. C. S., & Machado, A. A. S. C. (1998). Urea potentiometric biosensor based on urease immobilized on chitosan membreanes. Talanta, 47, 183\u2013191.","journal-title":"Talanta"},{"key":"9348_CR30","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.3390\/s7102251","volume":"7","author":"MS Alqasaimeh","year":"2007","unstructured":"Alqasaimeh, M. S., Heng, L., & Ahmad, M. (2007). A urea biosensor from stacked sol-gel films with \u0131mmobilized Nile Blue chromoionophore and urease enzyme. Sensors, 7, 2251\u20132262.","journal-title":"Sensors"},{"issue":"6","key":"9348_CR31","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.procbio.2006.01.017","volume":"41","author":"E Karaku\u015f","year":"2006","unstructured":"Karaku\u015f, E., Pekyard\u0131mc\u0131, \u015e., & K\u0131l\u0131\u00e7, E. (2006). Potentiometric bienzymatic biosensor based on PVC membrane containing palmitic acid for determination of creatine. Process Biochemistry, 41(6), 1371\u20131377.","journal-title":"Process Biochemistry"},{"issue":"3","key":"9348_CR32","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1080\/00032719408001088","volume":"27","author":"R Koncki","year":"1994","unstructured":"Koncki, R., Kopczewska, E., & Glab, S. (1994). Comparison of pH-membrane enzyme electrode for urea with covalently bound enzyme. Analytical Letters, 27(3), 475\u2013486.","journal-title":"Analytical Letters"},{"key":"9348_CR33","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0731-7085(99)00094-1","volume":"21","author":"R Koncki","year":"1999","unstructured":"Koncki, R., Chudvick, A., & Walcerz, I. (1999). Urea determination using pH-enzyme electrode. Journal of Pharmaceutical and Biomedical Analysis, 21, 51\u201357.","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"issue":"9","key":"9348_CR34","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1080\/00032719208018237","volume":"25","author":"SB Butt","year":"1992","unstructured":"Butt, S. B., & Camman, K. (1992). Enzyme urea biosensor based on a modified potentiometric PVC-nonactin membrane electrode for assay of urea in blood. Analytical Letters, 25(9), 1597\u20131615.","journal-title":"Analytical Letters"},{"key":"9348_CR35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0003-2670(95)00313-O","volume":"315","author":"I Walcerz","year":"1995","unstructured":"Walcerz, I., Koncki, R., Leszczynska, E., & Glab, S. (1995). Enzyme biosensors for urea determination based on an ionophore free pH membrane electrode. Analytica Chimica Acta, 315, 289\u2013296.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0003-2670(01)01441-6","volume":"452","author":"N Tinkilic","year":"2002","unstructured":"Tinkilic, N., Cubuk, O., & Isildak, I. (2002). Glucose and urea biosensor based on all solid-state PVC-NH2 membrane electrodes. Analytica Chimica Acta, 452, 29\u201334.","journal-title":"Analytica Chimica Acta"},{"key":"9348_CR37","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1016\/j.bios.2003.12.035","volume":"19","author":"B Lakard","year":"2004","unstructured":"Lakard, B., Herlem, G., Lakard, S., Antoniou, A., & Fahys, B. (2004). Urea potentiometric biosensor based on modified electrodes with urease immobilized on polyethylenimine films. Biosensors and Bioelectronics, 19, 1641\u20131647.","journal-title":"Biosensors and Bioelectronics"},{"key":"9348_CR38","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.jprot.2007.12.006","volume":"70","author":"SK Jha","year":"2008","unstructured":"Jha, S. K., Topkar, A., & D\u2019Souza, S. F. (2008). Development of potentiometric urea biosensor based on urease immobilized in PVA-PAA composite matrix for estimation of blood urea nitrogen (BUN). Journal of Biochemical and Biophysical Methods, 70, 1145\u20131150.","journal-title":"Journal of Biochemical and Biophysical Methods"},{"key":"9348_CR39","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/S0925-4005(99)00396-2","volume":"65","author":"Y Ru-Qin","year":"2000","unstructured":"Ru-Qin, Y., Zong-Rang, Z., & Guo-Li, S. (2000). Potentiometric sensors: Aspects of the recent development. Sensors and Actuators B, 65, 150\u2013153.","journal-title":"Sensors and Actuators B"},{"issue":"10","key":"9348_CR40","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1351\/pac200072101851","volume":"72","author":"Y Umezawa","year":"2000","unstructured":"Umezawa, Y., B\u00fchlmann, P., Umezawa, K., Tohda, K., & Amemiya, S. (2000). Pure and Applied Chemistry, 72(10), 1851\u20132082.","journal-title":"Pure and Applied Chemistry"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-011-9348-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12010-011-9348-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-011-9348-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T10:49:39Z","timestamp":1638442179000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12010-011-9348-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,1]]},"references-count":40,"journal-issue":{"issue":"5-6","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["9348"],"URL":"https:\/\/doi.org\/10.1007\/s12010-011-9348-2","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,1]]}}}