{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T11:10:04Z","timestamp":1780917004707,"version":"3.54.1"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2014,2,23]],"date-time":"2014-02-23T00:00:00Z","timestamp":1393113600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2014,2,23]],"date-time":"2014-02-23T00:00:00Z","timestamp":1393113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1007\/s12010-014-0771-z","type":"journal-article","created":{"date-parts":[[2014,2,22]],"date-time":"2014-02-22T04:48:56Z","timestamp":1393044536000},"page":"3635-3645","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression of Avian Influenza Virus (H5N1) Hemagglutinin and Matrix Protein 1 in Pichia pastoris and Evaluation of their Immunogenicity in Mice"],"prefix":"10.1007","volume":"172","author":[{"given":"M.","family":"Subathra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Santhakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sureddi","family":"Satyam Naidu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lakshmi Narasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. M. A.","family":"Senthilkumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil K.","family":"Lal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,2,23]]},"reference":[{"key":"771_CR1","doi-asserted-by":"publisher","first-page":"293","DOI":"10.2165\/00129785-200404050-00002","volume":"4","author":"R Lambkin","year":"2004","unstructured":"Lambkin, R., Novelli, P., Oxford, J., & Gelder, C. (2004). Human genetics and responses to influenza vaccination: clinical implication. American Journal of Pharmacogenomics, 4, 293\u2013298.","journal-title":"American Journal of Pharmacogenomics"},{"issue":"6","key":"771_CR2","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1016\/0264-410X(93)90314-N","volume":"11","author":"MP Johnson","year":"1993","unstructured":"Johnson, M. P., Meitin, C. A., Bender, B. S., & Small, P. A., Jr. (1993). Recombinant vaccinia immunization in the presence of passively administered antibody. Vaccine, 11(6), 665\u2013669.","journal-title":"Vaccine"},{"key":"771_CR3","doi-asserted-by":"publisher","first-page":"e1190","DOI":"10.1371\/journal.pone.0001190","volume":"2","author":"AT Heiny","year":"2007","unstructured":"Heiny, A. T., Miotto, O., Srinivasan, K. N., Khan, A. M., Zhang, G. L., Brusic, V., et al. (2007). Evolutionarily conserved protein sequences of influenza A viruses, avian and human, as vaccine targets. PLoS ONE, 2, e1190.","journal-title":"PLoS ONE"},{"key":"771_CR4","doi-asserted-by":"publisher","first-page":"6594","DOI":"10.1016\/j.vaccine.2006.05.051","volume":"24","author":"EGM Berkhoff","year":"2006","unstructured":"Berkhoff, E. G. M., de Wit, E., Geelhoed-Mieras, M. M., Boon, A. C. M., Symons, J., Fouchier, R. A. M., et al. (2006). Fitness costs limit escape from cytotoxic T lymphocytes by influenza A viruses. Vaccine, 24, 6594\u20136596.","journal-title":"Vaccine"},{"key":"771_CR5","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1073\/pnas.0609330104","volume":"104","author":"H-H Bui","year":"2007","unstructured":"Bui, H.-H., Peters, B., Assarsson, E., Mbawuike, I., & Sette, A. (2007). Ab and T cell epitopes of influenza A virus, knowledge and opportunities. Proceedings of the National Academy of Sciences of the United States of America, 104, 246\u2013251.","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"771_CR6","first-page":"3478","volume":"118","author":"LY Lee","year":"2008","unstructured":"Lee, L. Y., Ha, L. A., Simmons, C., de Jong, M. D., Chau, N. V., Schumacher, R., et al. (2008). Memory T cells established by seasonal human influenza A infection cross-react with avian influenza A (H5N1) inhealthy individuals. Journal of Clinical Investigation, 118, 3478\u20133490.","journal-title":"Journal of Clinical Investigation"},{"issue":"13","key":"771_CR7","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1056\/NEJMoa055778","volume":"354","author":"JJ Treanor","year":"2006","unstructured":"Treanor, J. J., Campbell, J. D., Zangwill, K. M., Rowe, T., & Wolff, M. (2006). Safety and immunogenicity of an inactivated subvirion influenza A (H5N1) vaccine. New England Journal of Medicine, 354(13), 1343\u20131351.","journal-title":"New England Journal of Medicine"},{"issue":"11","key":"771_CR8","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1016\/j.molmed.2006.09.003","volume":"12","author":"T Horimoto","year":"2006","unstructured":"Horimoto, T., & Kawaoka, Y. (2006). Strategies for developing vaccines against H5N1 influenza A viruses. Trends in Molecular Medicine, 12(11), 506\u2013514.","journal-title":"Trends in Molecular Medicine"},{"issue":"8","key":"771_CR9","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/S1473-3099(04)01105-3","volume":"4","author":"I Stephenson","year":"2004","unstructured":"Stephenson, I., Nicholson, K. G., Wood, J. M., Zambon, M. C., & Katz, J. M. (2004). Confronting the avian influenza threat: vaccine development for a potential pandemic. Lancet Infectious Diseases, 4(8), 499\u2013509.","journal-title":"Lancet Infectious Diseases"},{"issue":"10","key":"771_CR10","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1038\/nrmicro979","volume":"2","author":"JM Wood","year":"2004","unstructured":"Wood, J. M., & Robertson, J. S. (2004). From lethal virus to life-saving vaccine: developing inactivated vaccines for pandemic influenza. Nature Reviews Microbiology, 2(10), 842\u2013847.","journal-title":"Nature Reviews Microbiology"},{"issue":"9","key":"771_CR11","doi-asserted-by":"publisher","first-page":"3859","DOI":"10.1093\/nar\/15.9.3859","volume":"15","author":"JF Tschopp","year":"1987","unstructured":"Tschopp, J. F., Brust, P. F., Cregg, J. M., Stillman, C. A., & Gingeras, T. R. (1987). Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Research, 15(9), 3859\u20133876.","journal-title":"Nucleic Acids Research"},{"issue":"5","key":"771_CR12","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1038\/nbt0591-455","volume":"9","author":"JJ Clare","year":"1991","unstructured":"Clare, J. J., Rayment, F. B., Ballantine, S. P., Sreekrishna, K., & Romanos, M. A. (1991). High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology (N Y), 9(5), 455\u2013460.","journal-title":"Biotechnology (N Y)"},{"key":"771_CR13","doi-asserted-by":"publisher","first-page":"2284","DOI":"10.1128\/JCM.00921-07","volume":"45","author":"S Louisirirotchanakul","year":"2007","unstructured":"Louisirirotchanakul, S., Lerdsamran, H., Wiriyarat, W., Sangsiriwut, K., Chaichoune, K., Pooruk, P., et al. (2007). Erythrocyte binding preference of avian influenza H5N1 viruses. Journal of Clinical Microbiology, 45, 2284\u20132286.","journal-title":"Journal of Clinical Microbiology"},{"key":"771_CR14","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.vetmic.2010.05.031","volume":"146","author":"W Wiriyarat","year":"2010","unstructured":"Wiriyarat, W., Lerdsamran, H., Pooruk, P., Webster, R. G., Louisirirotchanakul, S., Ratanakorn, P., et al. (2010). Erythrocyte binding preference of 16 subtypes of low pathogenic avian influenza and 2009 pandemic influenza A (H1N1) viruses. Veterinary Microbiology, 146, 346\u2013349.","journal-title":"Veterinary Microbiology"},{"key":"771_CR15","doi-asserted-by":"publisher","first-page":"6234","DOI":"10.1016\/j.vaccine.2009.07.107","volume":"27","author":"Z Biesova","year":"2009","unstructured":"Biesova, Z., Miller, M. A., Schneerson, R., Shiloach, J., Green, K. Y., Robbins, J. B., et al. (2009). Preparation, characterization, and immunogenicity in mice of a recombinant influenza H5 hemagglutinin vaccine against the avian H5N1 A\/Vietnam\/1203\/2004 influenza virus. Vaccine, 27, 6234\u20136238.","journal-title":"Vaccine"},{"issue":"Pt 10","key":"771_CR16","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.1099\/0022-1317-80-10-2559","volume":"80","author":"Z Chen","year":"1999","unstructured":"Chen, Z., Yoshikawa, T., Kadowaki, S., Hagiwara, Y., Matsuo, K., Asanuma, H., et al. (1999). Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein. Journal of General Virology, 80(Pt 10), 2559\u20132564.","journal-title":"Journal of General Virology"},{"issue":"6","key":"771_CR17","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/0264-410X(95)00157-V","volume":"14","author":"T Deroo","year":"1996","unstructured":"Deroo, T., Jou, W. M., & Fiers, W. (1996). Recombinant neuraminidase vaccine protects against lethal influenza. Vaccine, 14(6), 561\u2013569.","journal-title":"Vaccine"},{"issue":"5","key":"771_CR18","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1128\/cdli.3.5.507-510.1996","volume":"3","author":"WA Keitel","year":"1996","unstructured":"Keitel, W. A., Cate, T. R., Atmar, R. L., Turner, C. S., Nino, D., Dukes, C. M., et al. (1996). Increasing doses of purified influenza virus hemagglutinin and subvirion vaccines enhance antibody responses in the elderly. Clinical and Diagnostic Laboratory Immunology, 3(5), 507\u2013510.","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"issue":"9","key":"771_CR19","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/0264-410X(93)90385-B","volume":"11","author":"HL Robinson","year":"1993","unstructured":"Robinson, H. L., Hunt, L. A., & Webster, R. G. (1993). Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA. Vaccine, 11(9), 957\u2013960.","journal-title":"Vaccine"},{"issue":"2","key":"771_CR20","first-page":"107","volume":"52","author":"F Fang","year":"2008","unstructured":"Fang, F., Cai, X. Q., Chang, H. Y., Wang, H. D., Yang, Z. D., & Chen, Z. (2008). Protection abilities of influenza B virus DNA vaccines expressing hemagglutinin, neuraminidase, or both in mice. Acta Virologica, 52(2), 107\u2013112.","journal-title":"Acta Virologica"},{"issue":"27","key":"771_CR21","doi-asserted-by":"publisher","first-page":"3681","DOI":"10.1016\/S0264-410X(01)00078-0","volume":"19","author":"K Okuda","year":"2001","unstructured":"Okuda, K., Ihata, A., Watabe, S., Okada, E., Yamakawa, T., Hamajima, K., et al. (2001). Protective immunity against influenza A virus induced by immunization with DNA plasmid containing influenza M gene. Vaccine, 19(27), 3681\u20133691.","journal-title":"Vaccine"},{"issue":"33","key":"771_CR22","doi-asserted-by":"publisher","first-page":"5481","DOI":"10.1016\/j.vaccine.2011.05.062","volume":"29","author":"KS Park","year":"2011","unstructured":"Park, K. S., Seo, Y. B., Lee, J. Y., Im, S. J., Seo, S. H., Song, M. S., et al. (2011). Complete protection against a H5N2 avian influenza virus by a DNA vaccine expressing a fusion protein of H1N1 HA and M2e. Vaccine, 29(33), 5481\u20135487.","journal-title":"Vaccine"},{"issue":"15\u201316","key":"771_CR23","doi-asserted-by":"publisher","first-page":"2073","DOI":"10.1016\/S0264-410X(98)00413-7","volume":"17","author":"BE Johansson","year":"1999","unstructured":"Johansson, B. E. (1999). Immunization with influenza A virus hemagglutinin and neuraminidase produced in recombinant baculovirus results in a balanced and broadened immune response superior to conventional vaccine. Vaccine, 17(15\u201316), 2073\u20132080.","journal-title":"Vaccine"},{"issue":"25\u201326","key":"771_CR24","doi-asserted-by":"publisher","first-page":"3706","DOI":"10.1016\/S0264-410X(03)00387-6","volume":"21","author":"T Jones","year":"2003","unstructured":"Jones, T., Allard, F., Cyr, S. L., Tran, S. P., Plante, M., Gauthier, J., et al. (2003). A nasal Proteosome influenza vaccine containing baculovirus-derived hemagglutinin induces protective mucosal and systemic immunity. Vaccine, 21(25\u201326), 3706\u20133712.","journal-title":"Vaccine"},{"key":"771_CR25","doi-asserted-by":"publisher","first-page":"2265","DOI":"10.1016\/S0264-410X(98)00494-0","volume":"17","author":"J Crawford","year":"1999","unstructured":"Crawford, J., Wilkinson, B., Vosnesensky, A., Smith, G., Garcia, M., Stone, H., et al. (1999). Baculovirus-derived hemagglutinin vaccines protect against lethal influenza infections by avian H5 and H7 subtypes. Vaccine, 17, 2265\u20132274.","journal-title":"Vaccine"},{"issue":"1","key":"771_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0166-0934(01)00332-9","volume":"98","author":"T Sugiura","year":"2001","unstructured":"Sugiura, T., Sugita, S., Imagawa, H., Kanaya, T., Ishiyama, S., Saeki, N., et al. (2001). Serological diagnosis of equine influenza using the hemagglutinin protein produced in a baculovirus expression system. Journal of Virological Methods, 98(1), 1\u20138.","journal-title":"Journal of Virological Methods"},{"issue":"6","key":"771_CR27","doi-asserted-by":"publisher","first-page":"1595","DOI":"10.1093\/infdis\/171.6.1595","volume":"171","author":"DC Powers","year":"1995","unstructured":"Powers, D. C., Smith, G. E., Anderson, E. L., Kennedy, D. J., Hackett, C. S., Wilkinson, B. E., et al. (1995). Influenza A virus vaccines containing purified recombinant H3 hemagglutinin are well tolerated and induce protective immune responses in healthy adults. Journal of Infectious Diseases, 171(6), 1595\u20131599.","journal-title":"Journal of Infectious Diseases"},{"issue":"13\u201314","key":"771_CR28","doi-asserted-by":"publisher","first-page":"1732","DOI":"10.1016\/S0264-410X(00)00395-9","volume":"19","author":"JJ Treanor","year":"2001","unstructured":"Treanor, J. J., Wilkinson, B. E., Masseoud, F., Hu-Primmer, J., Battaglia, R., O'Brien, D., et al. (2001). Safety and immunogenicity of a recombinant hemagglutinin vaccine for H5 influenza in humans. Vaccine, 19(13\u201314), 1732\u20131737.","journal-title":"Vaccine"},{"issue":"12","key":"771_CR29","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1016\/0264-410X(93)90040-5","volume":"11","author":"P Vanlandschoot","year":"1993","unstructured":"Vanlandschoot, P., Maertens, G., Jou, W. M., & Fiers, W. (1993). Recombinant secreted haemagglutinin protects mice against a lethal challenge of influenza virus. Vaccine, 11(12), 1185\u20131187.","journal-title":"Vaccine"},{"issue":"42","key":"771_CR30","doi-asserted-by":"publisher","first-page":"5393","DOI":"10.1016\/j.vaccine.2008.07.084","volume":"26","author":"K Mahmood","year":"2008","unstructured":"Mahmood, K., Bright, R. A., Mytle, N., Carter, D. M., Crevar, C. J., Achenbach, J. E., et al. (2008). H5N1 VLP vaccine induced protection in ferrets against lethal challenge with highly pathogenic H5N1 influenza viruses. Vaccine, 26(42), 5393\u20135399.","journal-title":"Vaccine"},{"issue":"19","key":"771_CR31","doi-asserted-by":"publisher","first-page":"3871","DOI":"10.1016\/j.vaccine.2007.01.106","volume":"25","author":"RA Bright","year":"2007","unstructured":"Bright, R. A., Carter, D. M., Daniluk, S., Toapanta, F. R., Ahmad, A., Gavrilov, V., et al. (2007). Influenza virus-like particles elicit broader immune responses than whole virion inactivated influenza virus or recombinant hemagglutinin. Vaccine, 25(19), 3871\u20133878.","journal-title":"Vaccine"},{"issue":"5","key":"771_CR32","doi-asserted-by":"publisher","first-page":"2814","DOI":"10.1128\/JVI.79.5.2814-2822.2005","volume":"79","author":"RA Fouchier","year":"2005","unstructured":"Fouchier, R. A., Munster, V., Wallensten, A., Bestebroer, T. M., Herfst, S., Smith, D., et al. (2005). Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. Journal of Virology, 79(5), 2814\u20132822.","journal-title":"Journal of Virology"},{"issue":"12","key":"771_CR33","doi-asserted-by":"publisher","first-page":"e14454","DOI":"10.1371\/journal.pone.0014454","volume":"5","author":"W Shi","year":"2010","unstructured":"Shi, W., Lei, F., Zhu, C., Sievers, F., & Higgins, D. G. (2010). A complete analysis of HA and NA genes of influenza A viruses. PLoS One, 5(12), e14454.","journal-title":"PLoS One"},{"issue":"6","key":"771_CR34","doi-asserted-by":"publisher","first-page":"e2432","DOI":"10.1371\/journal.pone.0002432","volume":"3","author":"S Rao","year":"2008","unstructured":"Rao, S., Kong, W. P., Wei, C. J., Yang, Z. Y., Nason, M., Styles, D., et al. (2008). Multivalent HA DNA vaccination protects against highly pathogenic H5N1 avian influenza infection in chickens and mice. PLoS One, 3(6), e2432.","journal-title":"PLoS One"},{"issue":"7","key":"771_CR35","doi-asserted-by":"publisher","first-page":"3514","DOI":"10.1128\/JVI.02052-06","volume":"81","author":"FS Quan","year":"2007","unstructured":"Quan, F. S., Huang, C., Compans, R. W., & Kang, S. M. (2007). Virus-like particle vaccine induces protective immunity against homologous and heterologous strains of influenza virus. Journal of Virology, 81(7), 3514\u20133524.","journal-title":"Journal of Virology"},{"issue":"29\u201330","key":"771_CR36","doi-asserted-by":"publisher","first-page":"3580","DOI":"10.1016\/j.vaccine.2008.05.001","volume":"26","author":"L Wang","year":"2008","unstructured":"Wang, L., Suarez, D. L., Pantin-Jackwood, M., Mibayashi, M., Garcia-Sastre, A., Saif, Y. M., et al. (2008). Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry. Vaccine, 26(29\u201330), 3580\u20133586.","journal-title":"Vaccine"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-014-0771-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12010-014-0771-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-014-0771-z","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-014-0771-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T04:16:29Z","timestamp":1744172189000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12010-014-0771-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,23]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["771"],"URL":"https:\/\/doi.org\/10.1007\/s12010-014-0771-z","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,23]]},"assertion":[{"value":"14 August 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}