{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T08:42:21Z","timestamp":1771231341076,"version":"3.50.1"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T00:00:00Z","timestamp":1454976000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1007\/s12010-016-2000-4","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T04:53:56Z","timestamp":1454993636000},"page":"375-382","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development of an in-House TaqMan Real-Time PCR-Based Method to Detect Residual Host Cell DNA in HBV Vaccine"],"prefix":"10.1007","volume":"179","author":[{"given":"Mahdi","family":"Paryan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mana","family":"Khodayar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Kia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Mohammadi\u2013Yeganeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hooman","family":"Kaghazian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,2,9]]},"reference":[{"issue":"3","key":"2000_CR1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1002\/hep.23190","volume":"50","author":"AS Lok","year":"2009","unstructured":"Lok, A. S., & McMahon, B. J. (2009). Chronic hepatitis B: update 2009. Hepatology, 50(3), 661\u2013662.","journal-title":"Hepatology"},{"issue":"2","key":"2000_CR2","first-page":"281","volume":"11","author":"L Romano","year":"2013","unstructured":"Romano, L., et al. (2013). Hepatitis B virus infection among first-time blood donors in Italy: prevalence and correlates between serological patterns and occult infection. Blood Transfusion, 11(2), 281\u2013288.","journal-title":"Blood Transfusion"},{"issue":"2","key":"2000_CR3","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1071\/SH10137","volume":"9","author":"J Wallace","year":"2012","unstructured":"Wallace, J., et al. (2012). Challenges to the effective delivery of health care to people with chronic hepatitis B in Australia. Sexual Health, 9(2), 131\u2013137.","journal-title":"Sexual Health"},{"issue":"7","key":"2000_CR4","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/S1473-3099(02)00315-8","volume":"2","author":"JH Kao","year":"2002","unstructured":"Kao, J. H., & Chen, D. S. (2002). Global control of hepatitis B virus infection. Lancet Infectious Diseases, 2(7), 395\u2013403.","journal-title":"Lancet Infectious Diseases"},{"issue":"47","key":"2000_CR5","doi-asserted-by":"crossref","first-page":"6550","DOI":"10.1016\/j.vaccine.2009.08.048","volume":"27","author":"X Liang","year":"2009","unstructured":"Liang, X., et al. (2009). Epidemiological serosurvey of hepatitis B in China\u2014declining HBV prevalence due to hepatitis B vaccination. Vaccine, 27(47), 6550\u20136557.","journal-title":"Vaccine"},{"issue":"6","key":"2000_CR6","doi-asserted-by":"crossref","first-page":"683","DOI":"10.5483\/BMBRep.2005.38.6.683","volume":"38","author":"H Bo","year":"2005","unstructured":"Bo, H., et al. (2005). Expression of hepatitis B virus S gene in Pichia pastoris and application of the product for detection of anti-HBs antibody. Journal of Biochemistry and Molecular Biology, 38(6), 683\u2013689.","journal-title":"Journal of Biochemistry and Molecular Biology"},{"key":"2000_CR7","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1186\/1743-422X-4-106","volume":"4","author":"SZ Salahuddin","year":"2007","unstructured":"Salahuddin, S. Z., et al. (2007). The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell. Virology Journal, 4, 106.","journal-title":"Virology Journal"},{"issue":"1","key":"2000_CR8","doi-asserted-by":"crossref","first-page":"37","DOI":"10.2225\/vol10-issue1-fulltext-15","volume":"10","author":"A Beldarra\u00edn Iznaga","year":"2007","unstructured":"Beldarra\u00edn Iznaga, A., et al. (2007). DNA removal from a purification process of recombinant hepatitis B surface antigen. Electronic Journal of Biotechnology, 10(1), 37\u201347.","journal-title":"Electronic Journal of Biotechnology"},{"key":"2000_CR9","doi-asserted-by":"crossref","unstructured":"Yang, H. and J. Zhang, A Bayesian approach to residual host cell DNA safety assessment. PDA J Pharm Sci Technol, 2016.","DOI":"10.5731\/pdajpst.2015.006296"},{"issue":"2\u20133","key":"2000_CR10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0168-1656(99)00201-1","volume":"77","author":"E Hardy","year":"2000","unstructured":"Hardy, E., et al. (2000). Large-scale production of recombinant hepatitis B surface antigen from Pichia pastoris. Journal of Biotechnology, 77(2\u20133), 157\u2013167.","journal-title":"Journal of Biotechnology"},{"issue":"4","key":"2000_CR11","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.jpba.2009.01.022","volume":"49","author":"Y Ikeda","year":"2009","unstructured":"Ikeda, Y., Iwakiri, S., & Yoshimori, T. (2009). Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain \u201cPicoGreen\u201d. Journal of Pharmaceutical and Biomedical Analysis, 49(4), 997\u20131002.","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"issue":"8","key":"2000_CR12","doi-asserted-by":"crossref","first-page":"e43246","DOI":"10.1371\/journal.pone.0043246","volume":"7","author":"N Pripuzova","year":"2012","unstructured":"Pripuzova, N., et al. (2012). Development of real-time PCR array for simultaneous detection of eight human blood-borne viral pathogens. PloS One, 7(8), e43246.","journal-title":"PloS One"},{"issue":"2","key":"2000_CR13","first-page":"106","volume":"17","author":"Z Alizadeh","year":"2014","unstructured":"Alizadeh, Z., Milani, S., & Sharifi, Z. (2014). Occult hepatitis B virus infection among Iranian blood donors: a preliminary study. Archives of Iranian Medicine, 17(2), 106\u2013107.","journal-title":"Archives of Iranian Medicine"},{"issue":"4","key":"2000_CR14","doi-asserted-by":"crossref","first-page":"e17632","DOI":"10.5812\/hepatmon.17632","volume":"14","author":"M Heiat","year":"2014","unstructured":"Heiat, M., Ranjbar, R., & Alavian, S. M. (2014). Classical and modern approaches used for viral hepatitis diagnosis. Hepatitis Monthly, 14(4), e17632.","journal-title":"Hepatitis Monthly"},{"issue":"10","key":"2000_CR15","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.4014\/jmb.1004.04035","volume":"20","author":"DH Lee","year":"2010","unstructured":"Lee, D. H., et al. (2010). Quantitative detection of residual E. coli host cell DNA by real-time PCR. Journal of Microbiology and Biotechnology, 20(10), 1463\u20131470.","journal-title":"Journal of Microbiology and Biotechnology"},{"key":"2000_CR16","doi-asserted-by":"crossref","unstructured":"Paryan, M., et al. Simultaneous detection and genotype determination of HSV 1 and 2 by real-time PCR using melting curve analysis and a unique pair of primers. Appl Immunohistochem Mol Morphol, 2015","DOI":"10.1097\/PAI.0000000000000282"},{"issue":"5","key":"2000_CR17","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.1007\/s12010-014-1442-9","volume":"175","author":"F Riahi","year":"2015","unstructured":"Riahi, F., et al. (2015). Evaluation of point mutation detection in Mycobacterium tuberculosis with isoniazid resistance using real-time PCR and TaqMan probe assay. Applied Biochemistry and Biotechnology, 175(5), 2447\u20132455.","journal-title":"Applied Biochemistry and Biotechnology"},{"issue":"1","key":"2000_CR18","first-page":"8","volume":"4","author":"M Paryan","year":"2012","unstructured":"Paryan, M., et al. (2012). Design and development of an in-house multiplex RT-PCR assay for simultaneous detection of HIV-1 and HCV in plasma samples. Iran Journal Microbiology, 4(1), 8\u201314.","journal-title":"Iran Journal Microbiology"},{"issue":"26","key":"2000_CR19","doi-asserted-by":"crossref","first-page":"9658","DOI":"10.1021\/jf061987c","volume":"54","author":"CB Andersen","year":"2006","unstructured":"Andersen, C. B., et al. (2006). Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean. Journal of Agricultural and Food Chemistry, 54(26), 9658\u20139663.","journal-title":"Journal of Agricultural and Food Chemistry"},{"issue":"8","key":"2000_CR20","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1089\/hum.1998.9.8-1173","volume":"9","author":"R Lahijani","year":"1998","unstructured":"Lahijani, R., et al. (1998). Quantitation of host cell DNA contaminate in pharmaceutical-grade plasmid DNA using competitive polymerase chain reaction and enzyme-linked immunosorbent assay. Human Gene Therapy, 9(8), 1173\u20131180.","journal-title":"Human Gene Therapy"},{"issue":"2","key":"2000_CR21","first-page":"40","volume":"3","author":"T Wolter","year":"2005","unstructured":"Wolter, T., & Richter, A. (2005). Assays for controlling host-cell impurities in biopharmaceuticals. BioProcess International, 3(2), 40\u201346.","journal-title":"BioProcess International"},{"issue":"5","key":"2000_CR22","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.2144\/02325dd06","volume":"32","author":"X Ji","year":"2002","unstructured":"Ji, X., Lee, K., & DiPaolo, B. (2002). High-sensitivity hybridization assay for quantitation of residual E. coli DNA. Biotechniques, 32(5), 1162\u20131167.","journal-title":"Biotechniques"},{"issue":"14","key":"2000_CR23","doi-asserted-by":"crossref","first-page":"2656","DOI":"10.1016\/j.vaccine.2005.11.066","volume":"24","author":"KY Wang","year":"2006","unstructured":"Wang, K. Y., et al. (2006). 16S rRNA gene probe quantitates residual host cell DNA in pharmaceutical-grade plasmid DNA. Vaccine, 24(14), 2656\u20132661.","journal-title":"Vaccine"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-016-2000-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12010-016-2000-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-016-2000-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T07:07:18Z","timestamp":1567580838000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12010-016-2000-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,9]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["2000"],"URL":"https:\/\/doi.org\/10.1007\/s12010-016-2000-4","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,9]]}}}