{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T15:24:24Z","timestamp":1773415464097,"version":"3.50.1"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2017,9,16]],"date-time":"2017-09-16T00:00:00Z","timestamp":1505520000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2017,9,16]],"date-time":"2017-09-16T00:00:00Z","timestamp":1505520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2018,3]]},"DOI":"10.1007\/s12010-017-2592-3","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T09:58:21Z","timestamp":1652176701000},"page":"898-908","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Colorimetric Detection of Unamplified Rift Valley Fever Virus Genetic Material Using Unmodified Gold Nanoparticles"],"prefix":"10.1007","volume":"184","author":[{"given":"Mostafa R.","family":"Zaher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8267-2702","authenticated-orcid":false,"given":"Hanaa A.","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8267-2702","authenticated-orcid":false,"given":"Kareem E. Z.","family":"Hamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reham H.","family":"Tammam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,9,16]]},"reference":[{"key":"2592_CR1","doi-asserted-by":"crossref","unstructured":"Chevalier, V., P\u00e9pin, M., Pl\u00e9e, L., & Lancelot, R. (2010). Rift Valley fever\u2014a threat for Europe? Eurosurveillance Edition, 15(10), 30-40\u00a0","DOI":"10.2807\/ese.15.10.19506-en"},{"issue":"9","key":"2592_CR2","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1007\/BF03401814","volume":"3","author":"RM Elliott","year":"1997","unstructured":"Elliott, R. M. (1997). Emerging viruses: the Bunyaviridae. Molecular Medicine, 3(9), 572\u2013577.","journal-title":"Molecular Medicine"},{"issue":"4","key":"2592_CR3","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1002\/path.1700340418","volume":"34","author":"R Daubney","year":"1931","unstructured":"Daubney, R., Hudson, J. R., & Garnham, P. C. (1931). Enzootic hepatitis or rift valley fever. An undescribed virus disease of sheep cattle and man from East Africa. The Journal of Pathology and Bacteriology, 34(4), 545\u2013579. https:\/\/doi.org\/10.1002\/path.1700340418.","journal-title":"The Journal of Pathology and Bacteriology"},{"issue":"3","key":"2592_CR4","doi-asserted-by":"publisher","first-page":"23","DOI":"10.15406\/jdvar.2015.02.00034","volume":"2","author":"MZ Sayed-Ahmed","year":"2015","unstructured":"Sayed-Ahmed, M. Z. (2015). Epidemic situation of Rift Valley fever in Egypt and Saudi Arabia. Journal of Dairy, Veterinary & Animal Research, 2(3), 23\u201325. https:\/\/doi.org\/10.15406\/jdvar.2015.02.00034.","journal-title":"Journal of Dairy, Veterinary & Animal Research"},{"issue":"8","key":"2592_CR5","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.1086\/378747","volume":"37","author":"TA Madani","year":"2003","unstructured":"Madani, T. A., Al-Mazrou, Y. Y., Al-Jeffri, M. H., Mishkhas, A. A., Al-Rabeah, A. M., Turkistani, A. M., et al. (2003). Rift Valley fever epidemic in Saudi Arabia: epidemiological, clinical, and laboratory characteristics. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America, 37(8), 1084\u20131092. https:\/\/doi.org\/10.1086\/378747.","journal-title":"Clinical infectious diseases: an official publication of the Infectious Diseases Society of America"},{"key":"2592_CR6","unstructured":"Shaif, A. (2011). The epidemiology of rift valley fever in Yemen and the risk of re-introduction from the horn of Africa. L\u2019\u00e9pid\u00e9miologie de la fi\u00e8vre de la vall\u00e9e du Rift au Yemen, PhD thesis,\u00a0University of Li\u00e8ge.\u00a0Li\u00e8ge,\u00a0Belgian"},{"key":"2592_CR7","doi-asserted-by":"publisher","unstructured":"Abdel-Wahab, K. S., El Baz, L. M., El-Tayeb, E. M., Omar, H., Ossman, M. A, & Yasin, W. (1978). Rift Valley Fever virus infections in Egypt: pathological and virological findings in man. Transactions of the Royal Society of Tropical Medicine and Hygiene, 72(4), 392\u2013396. doi:http:\/\/dx.doi.org\/10.1016\/0035-9203(78)90134-7","DOI":"10.1016\/0035-9203(78)90134-7"},{"key":"2592_CR8","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1186\/1743-422X-8-532","volume":"8","author":"S Ahmed Kamal","year":"2011","unstructured":"Ahmed Kamal, S., & Kamal, S. A. (2011). Observations on rift valley fever virus and vaccines in Egypt. Virology Journal, 8, 532. https:\/\/doi.org\/10.1186\/1743-422x-8-532.","journal-title":"Virology Journal"},{"issue":"5","key":"2592_CR9","doi-asserted-by":"publisher","first-page":"493","DOI":"10.3390\/v3050493","volume":"3","author":"T Ikegami","year":"2011","unstructured":"Ikegami, T., & Makino, S. (2011). The pathogenesis of rift valley fever. Virus, 3(5), 493\u2013519. https:\/\/doi.org\/10.3390\/v3050493.","journal-title":"Virus"},{"key":"2592_CR10","unstructured":"Rift valley fever (RVF), transmission.\u00a02013. \u00a0Avaliable from www.cdc.gov\/vhf\/rvf\/transmission\/index.html."},{"issue":"42","key":"2592_CR11","doi-asserted-by":"publisher","first-page":"5520","DOI":"10.1016\/j.vaccine.2015.08.020","volume":"33","author":"KL Mansfield","year":"2015","unstructured":"Mansfield, K. L., Banyard, A. C., McElhinney, L., Johnson, N., Horton, D. L., Hern\u00e1ndez-Triana, L. M., & Fooks, A. R. (2015). Rift Valley fever virus: a review of diagnosis and vaccination, and implications for emergence in Europe. Vaccine, 33(42), 5520\u20135531. https:\/\/doi.org\/10.1016\/j.vaccine.2015.08.020.","journal-title":"Vaccine"},{"issue":"2","key":"2592_CR12","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.ijid.2009.03.029","volume":"14","author":"AA Adam","year":"2010","unstructured":"Adam, A. A., Karsany, M. S., & Adam, I. (2010). Manifestations of severe Rift Valley fever in Sudan. International Journal of Infectious Diseases, 14(2), 179\u2013180. https:\/\/doi.org\/10.1016\/j.ijid.2009.03.029.","journal-title":"International Journal of Infectious Diseases"},{"issue":"1","key":"2592_CR13","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.virusres.2012.07.019","volume":"169","author":"D Mwaengo","year":"2012","unstructured":"Mwaengo, D., Lorenzo, G., Iglesias, J., Warigia, M., Sang, R., Bishop, R. P., & Brun, A. (2012). Detection and identification of Rift Valley fever virus in mosquito vectors by quantitative real-time PCR. Virus Research, 169(1), 137\u2013143. https:\/\/doi.org\/10.1016\/j.virusres.2012.07.019.","journal-title":"Virus Research"},{"issue":"2","key":"2592_CR14","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.jcv.2007.10.001","volume":"41","author":"M Weidmann","year":"2008","unstructured":"Weidmann, M., Sanchez-Seco, M. P., Sall, A. A., Ly, P. O., Thiongane, Y., L\u00f4, M. M., et al. (2008). Rapid detection of important human pathogenic phleboviruses. Journal of Clinical Virology, 41(2), 138\u2013142. https:\/\/doi.org\/10.1016\/j.jcv.2007.10.001.","journal-title":"Journal of Clinical Virology"},{"issue":"6","key":"2592_CR15","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1128\/jcm.17.6.1026-1031.1983","volume":"17","author":"B Niklasson","year":"1983","unstructured":"Niklasson, B., Grandien, M., Peters, C. J., & Gargan, T. P. (1983). Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay. Egypt Journal of Clinical Microbiology, 17(6), 1026\u20131031.","journal-title":"Egypt Journal of Clinical Microbiology"},{"issue":"11","key":"2592_CR16","doi-asserted-by":"publisher","first-page":"3653","DOI":"10.1128\/JCM.01188-08","volume":"46","author":"CN Peyrefitte","year":"2008","unstructured":"Peyrefitte, C. N., Boubis, L., Coudrier, D., Bouloy, M., Grandadam, M., Tolou, H. J., & Plumet, S. (2008). Real-time reverse-transcription loop-mediated isothermal amplification for rapid detection of Rift Valley fever virus. Journal of Clinical Microbiology, 46(11), 3653\u20133659. https:\/\/doi.org\/10.1128\/JCM.01188-08.","journal-title":"Journal of Clinical Microbiology"},{"issue":"4","key":"2592_CR17","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.jcv.2012.05.006","volume":"54","author":"M Euler","year":"2012","unstructured":"Euler, M., Wang, Y., Nentwich, O., Piepenburg, O., Hufert, F. T., & Weidmann, M. (2012). Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus. Journal of Clinical Virology, 54(4), 308\u2013312. https:\/\/doi.org\/10.1016\/j.jcv.2012.05.006.","journal-title":"Journal of Clinical Virology"},{"key":"2592_CR18","doi-asserted-by":"crossref","unstructured":"Med, I. J., & Res, N. (2016). Nanoparticles: from diagnosis to therapy. International Journal of Medical Nano Research, 3(1), 1\u20135.","DOI":"10.23937\/2378-3664\/1410015"},{"key":"2592_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1745-6673-2-16","volume":"6","author":"SS Suri","year":"2007","unstructured":"Suri, S. S., Fenniri, H., & Singh, B. (2007). Nanotechnology-based drug delivery systems. Journal of Occupational Medicine and Toxicology, 6, 1\u20136. https:\/\/doi.org\/10.1186\/1745-6673-2-16.","journal-title":"Journal of Occupational Medicine and Toxicology"},{"issue":"5","key":"2592_CR20","first-page":"511","volume":"9","author":"SH Radwan","year":"2009","unstructured":"Radwan, S. H., & Azzazy, H. M. (2009). Gold nanoparticles for molecular diagnostics. Expert Reviews, 9(5), 511\u2013524.","journal-title":"Expert Reviews"},{"key":"2592_CR21","doi-asserted-by":"publisher","unstructured":"Huang, X., & El-sayed, M. A. (2010). Gold nanoparticles: optical properties and implementations in cancer diagnosis and photothermal therapy. Journal of Advanced Research, 1(1), 13\u201328. https:\/\/doi.org\/10.1016\/j.jare.2010.02.002.","DOI":"10.1016\/j.jare.2010.02.002"},{"key":"2592_CR22","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.talanta.2005.10.017","volume":"69","author":"X Shen","year":"2006","unstructured":"Shen, X., Jiang, L., Liang, H., Lu, X., Zhang, L., & Liu, X. (2006). Determination of 6-mercaptopurine based on the fluorescence enhancement of Au nanoparticles. Talanta, 69, 456\u2013462. https:\/\/doi.org\/10.1016\/j.talanta.2005.10.017.","journal-title":"Talanta"},{"key":"2592_CR23","doi-asserted-by":"publisher","first-page":"1643","DOI":"10.1021\/ja021096v","volume":"25","author":"R Jin","year":"2003","unstructured":"Jin, R., Wu, G., Li, Z., Mirkin, C. A., & Schatz, G. C. (2003). What controls the melting properties of DNA-linked gold nanoparticle assemblies? Have been used as probes in a variety of DNA detection methods. Journal of the American Chemical Society, 25, 1643\u20131654.","journal-title":"Journal of the American Chemical Society"},{"issue":"39","key":"2592_CR24","doi-asserted-by":"publisher","first-page":"14036","DOI":"10.1073\/pnas.0406115101","volume":"101","author":"H Li","year":"2004","unstructured":"Li, H., & Rothberg, L. (2004). Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proceeding of the National Academy of Sciences of the United States of America, 101(39), 14036\u201314039.","journal-title":"Proceeding of the National Academy of Sciences of the United States of America"},{"issue":"13\u201314","key":"2592_CR25","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.1016\/j.clinbiochem.2010.07.001","volume":"43","author":"SM Shawky","year":"2010","unstructured":"Shawky, S. M., Bald, D., & Azzazy, H. M. E. (2010). Direct detection of unamplified hepatitis C virus RNA using unmodified gold nanoparticles. Clinical Biochemistry, 43(13\u201314), 1163\u20131168. https:\/\/doi.org\/10.1016\/j.clinbiochem.2010.07.001.","journal-title":"Clinical Biochemistry"},{"key":"2592_CR26","doi-asserted-by":"publisher","unstructured":"Verma, M. S., Rogowski, J. L., Jones, L., & Gu, F. X. (2015). Colorimetric biosensing of pathogens using gold nanoparticles. Biotechnology Advances. https:\/\/doi.org\/10.1016\/j.biotechadv.2015.03.003.","DOI":"10.1016\/j.biotechadv.2015.03.003"},{"issue":"1\u20132","key":"2592_CR27","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.clinbiochem.2013.10.022","volume":"47","author":"S Eissa","year":"2014","unstructured":"Eissa, S., Shawky, S. M., Matboli, M., Mohamed, S., & Azzazy, H. M. E. (2014). Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis. Clinical Biochemistry, 47(1\u20132), 104\u2013110. https:\/\/doi.org\/10.1016\/j.clinbiochem.2013.10.022.","journal-title":"Clinical Biochemistry"},{"issue":"12","key":"2592_CR28","doi-asserted-by":"publisher","first-page":"3989","DOI":"10.1039\/C5AN00407A","volume":"140","author":"Y Liu","year":"2015","unstructured":"Liu, Y., Zhang, L., Wei, W., Zhao, H., Zhou, Z., Zhang, Y., & Liu, S. (2015). Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles. The Analyst, 140(12), 3989\u20133995. https:\/\/doi.org\/10.1039\/C5AN00407A.","journal-title":"The Analyst"},{"issue":"12","key":"2592_CR29","doi-asserted-by":"publisher","first-page":"6509","DOI":"10.1021\/acs.analchem.6b01258","volume":"88","author":"Y Liu","year":"2016","unstructured":"Liu, Y., Wei, M., Zhang, L., Zhang, Y., Wei, W., Yin, L., et al. (2016). Chiroplasmonic assemblies of gold nanoparticles for ultrasensitive detection of 8-hydroxy-2\u2032-deoxyguanosine in human serum sample. Analytical Chemistry, 88(12), 6509\u20136514. https:\/\/doi.org\/10.1021\/acs.analchem.6b01258.","journal-title":"Analytical Chemistry"},{"issue":"2","key":"2592_CR30","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1007\/s12010-014-1085-x","volume":"174","author":"S Eissa","year":"2014","unstructured":"Eissa, S., Azzazy, H. M. E., Matboli, M., Shawky, S. M., Said, H., & Anous, F. A. (2014). The prognostic value of histidine-rich glycoprotein RNA in breast tissue using unmodified gold nanoparticles assay. Applied Biochemistry and Biotechnology, 174(2), 751\u2013761. https:\/\/doi.org\/10.1007\/s12010-014-1085-x.","journal-title":"Applied Biochemistry and Biotechnology"},{"issue":"105","key":"2592_CR31","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/physci241020a0","volume":"241","author":"G Frens","year":"1973","unstructured":"Frens, G. (1973). Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature Physical Science, 241(105), 20\u201322. https:\/\/doi.org\/10.1038\/physci241020a0.","journal-title":"Nature Physical Science"},{"issue":"34","key":"2592_CR32","doi-asserted-by":"publisher","first-page":"9023","DOI":"10.1016\/j.biomaterials.2010.08.012","volume":"31","author":"SK Balasubramanian","year":"2010","unstructured":"Balasubramanian, S. K., Yang, L., Yung, L. L., Ong, C., & Ong, W. (2010). Biomaterials characterization, purification and stability of gold nanoparticles. Biomaterials, 31(34), 9023\u20139030. https:\/\/doi.org\/10.1016\/j.biomaterials.2010.08.012.","journal-title":"Biomaterials"},{"key":"2592_CR33","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.colsurfb.2006.08.005","volume":"58","author":"X Liu","year":"2007","unstructured":"Liu, X., Atwater, M., Wang, J., & Huo, Q. (2007). Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. Colloids and Surfaces B: Biointerfaces, 58, 3\u20137. https:\/\/doi.org\/10.1016\/j.colsurfb.2006.08.005.","journal-title":"Colloids and Surfaces B: Biointerfaces"},{"issue":"12","key":"2592_CR34","doi-asserted-by":"publisher","first-page":"4456","DOI":"10.1128\/JCM.39.12.4456-4461.2001","volume":"39","author":"S Garcia","year":"2001","unstructured":"Garcia, S., Crance, J. M., Billecocq, A., Peinnequin, A., Jouan, A., Bouloy, M., & Garin, D. (2001). Quantitative real-time PCR detection of Rift Valley fever virus and its application to evaluation of antiviral compounds. Journal of Clinical Microbiology, 39(12), 4456\u20134461. https:\/\/doi.org\/10.1128\/JCM.39.12.4456.","journal-title":"Journal of Clinical Microbiology"},{"key":"2592_CR35","doi-asserted-by":"publisher","first-page":"100891","DOI":"10.1039\/C5RA16326A","volume":"5","author":"Z Liu","year":"2015","unstructured":"Liu, Z., Xia, X., Yang, C., & Wang, L. (2015). Visual detection of maize chlorotic mottle virus using unmodified gold nanoparticles. RSC Advances, 5, 100891\u2013100897. https:\/\/doi.org\/10.1039\/C5RA16326A.","journal-title":"RSC Advances"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-017-2592-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12010-017-2592-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-017-2592-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T12:12:53Z","timestamp":1652184773000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12010-017-2592-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,16]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["2592"],"URL":"https:\/\/doi.org\/10.1007\/s12010-017-2592-3","relation":{},"ISSN":["0273-2289"],"issn-type":[{"value":"0273-2289","type":"print"}],"subject":[],"published":{"date-parts":[[2017,9,16]]},"assertion":[{"value":"11 June 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 August 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"All authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}