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Biolipasa-R, a commercially available and low-cost lipase, was employed for enzymatic transesterification. Response surface methodology was applied to optimize the enzymatic transesterification process. The optimal conditions for biodiesel production, which comprised 42% lipase concentration (per weight of oil), 32% water content (per weight of oil), a methanol to oil molar ratio of 3:1, pH 7.0 and reaction temperature 30\u00b0C, resulted in the highest fatty acid methyl ester (FAME) content (71.3%). Subsequently, the synergistic effect of two lipases with different regioselectivities under the optimum transesterification conditions was studied, aiming at the enhancement of process efficiency. The transesterification efficiency of immobilized Biolipasa-R was determined and compared to that of Biolipasa-R in its free form. The results revealed a good performance on FAME content (66.5%), while the recycling of immobilized lipase resulted in a decrease in transesterification efficiency after three consecutive uses.<\/jats:p>","DOI":"10.1007\/s12010-024-04941-3","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T05:02:06Z","timestamp":1714626126000},"page":"8168-8189","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Optimization of Enzymatic Transesterification of Acid Oil for Biodiesel Production Using a Low-Cost Lipase: The Effect of Transesterification Conditions and the Synergy of Lipases with Different Regioselectivity"],"prefix":"10.1007","volume":"196","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3465-0633","authenticated-orcid":false,"given":"Alexandra","family":"Moschona","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Androniki","family":"Spanou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5811-368X","authenticated-orcid":false,"given":"Ioannis V.","family":"Pavlidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1463-519X","authenticated-orcid":false,"given":"Anastasios J.","family":"Karabelas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7180-9291","authenticated-orcid":false,"given":"Sotiris I.","family":"Patsios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"4941_CR1","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1007\/s12010-008-8377-y","volume":"160","author":"Y Huang","year":"2010","unstructured":"Huang, Y., Zheng, H., & Yan, Y. 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