{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:42:40Z","timestamp":1791434560364,"version":"4.3.3"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["305207\/2023-7"],"award-info":[{"award-number":["305207\/2023-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["407595\/2022-8"],"award-info":[{"award-number":["407595\/2022-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["407595\/2022-8"],"award-info":[{"award-number":["407595\/2022-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["407595\/2022-8"],"award-info":[{"award-number":["407595\/2022-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>This work focuses on improving the stability of <jats:italic>Burkholderia cepacia<\/jats:italic> lipase immobilized on styrene\u2013divinylbenzene by using chemical additives and a cost-effective physical adsorption method. Ethanol pretreatment of the supports proved essential for maintaining enzyme activity. The optimal conditions for immobilization were achieved at a 1:1 support-to-enzyme ratio, pH 8, 200\u00a0rpm, and 60\u00a0\u00b0C. Combinations of the additives glutaraldehyde, polyethylene glycol 1500, and Triton X-100 were examined for activation treatment of supports before immobilization. Concentrations of 2.5% (w\/v) of polyethylene glycol 1500 and 0.5% (v\/v) of Triton X-100 were used to maximize biocatalyst activity. We show that the activated biocatalyst yielded up to 950% more hexyl acetate than non-activated control after 12 reaction cycles. Fourier transform infrared spectroscopy and scanning electron microscopy confirmed the effective immobilization of the <jats:italic>Burkholderia cepacia<\/jats:italic> lipase. This study introduces a scalable and sustainable method for creating robust biocatalysts aimed at producing value-added chemicals, thereby advancing green chemistry in the flavor industry.<\/jats:p>","DOI":"10.1007\/s12010-025-05277-2","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T05:17:50Z","timestamp":1749014270000},"page":"5344-5361","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Stability of Burkholderia cepacia Lipase Immobilized on Styrene\u2013Divinylbenzene Activated with Glutaraldehyde, Triton X-100, and Polyethylene Glycol for the Green Synthesis of Hexyl Acetate"],"prefix":"10.1007","volume":"197","author":[{"given":"Wellington M.","family":"Correa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wislei R.","family":"Os\u00f3rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ausdinir D.","family":"Bortolozo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Poloni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovana S.","family":"Padilha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"issue":"4","key":"5277_CR1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1080\/02652048.2019.1626927","volume":"36","author":"WC Moreira","year":"2019","unstructured":"Moreira, W. C., Elias, A. L. P., Os\u00f3rio, W. R., & Padilha, G. S. (2019). Alternative method to improve the ethyl valerate yield using an immobilized Burkholderia cepacia lipase. Journal of Microencapsulation, 36(4), 327\u2013337. https:\/\/doi.org\/10.1080\/02652048.2019.1626927","journal-title":"Journal of Microencapsulation"},{"key":"5277_CR2","doi-asserted-by":"publisher","first-page":"3071","DOI":"10.1007\/s10973-021-10714-5","volume":"147","author":"WM Correa","year":"2021","unstructured":"Correa, W. M., Bortolozo, A. D., Os\u00f3rio, W. R., & Padilha, G. S. (2021). Physical adsorption used to the immobilization of Burkholderia cepacia lipase into powder polymeric supports. Journal of Thermal Analysis and Calorimetry, 147, 3071\u20133081. https:\/\/doi.org\/10.1007\/s10973-021-10714-5","journal-title":"Journal of Thermal Analysis and Calorimetry"},{"key":"5277_CR3","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/B978-0-12-821406-0.00025-4","volume":"2","author":"PA Basha","year":"2021","unstructured":"Basha, P. A. (2021). Oil degrading lipases and their role in environmental pollution. Recent Developments in Applied Microbiology and Biochemistry, 2, 269\u2013277. https:\/\/doi.org\/10.1016\/B978-0-12-821406-0.00025-4","journal-title":"Recent Developments in Applied Microbiology and Biochemistry"},{"key":"5277_CR4","doi-asserted-by":"publisher","unstructured":"Carvalho, W. C. A., Luiz J. H. H., Fernandez-Lafuente, R., Hirata, D. B., Mendes, A. A. (2021). Eco-friendly production of trimethylolpropane triesters from refined and used soybean cooking oils using an immobilized low-cost lipase (Eversa>\u00ae Transform 2.0) as heterogeneous catalyst. Biomass and Bioenergy, 155, 106302. https:\/\/doi.org\/10.1016\/j.biombioe.2021.106302.","DOI":"10.1016\/j.biombioe.2021.106302"},{"key":"5277_CR5","doi-asserted-by":"publisher","first-page":"105819","DOI":"10.1016\/j.pep.2021.105819","volume":"180","author":"S Gurkok","year":"2021","unstructured":"Gurkok, S., & Ozdal, M. (2021). Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry. Protein Expression and Purification, 180, 105819. https:\/\/doi.org\/10.1016\/j.pep.2021.105819","journal-title":"Protein Expression and Purification"},{"key":"5277_CR6","doi-asserted-by":"publisher","first-page":"109935","DOI":"10.1016\/j.enzmictec.2021.109935","volume":"152","author":"Z Qin","year":"2022","unstructured":"Qin, Z., Feng, N., Ma, Y., Li, Y., Xu, L., Wang, Y., Fei, X., & Tian, J. (2022). A lipase\/poly (ionic liquid)-styrene microspheres\/PVA composite hydrogel for esterification application. Enzyme and Microbial Technology, 152, 109935. https:\/\/doi.org\/10.1016\/j.enzmictec.2021.109935","journal-title":"Enzyme and Microbial Technology"},{"key":"5277_CR7","doi-asserted-by":"publisher","unstructured":"Chen, M., She, W., Zhao, X., Chen, C., Zhu, B., Sun, Y., Yao, Z. (2024). Immobilization of Thermomyces lanuginosus lipase in a novel polysaccharide\u2011based hydrogel by a two\u2011step crosslinking method and its use in the lauroylation of \u03b1\u2011arbutin. Bioresources and Bioprocessing, 11(7), 1\u201314. https:\/\/doi.org\/10.1186\/s40643-023-00721-9","DOI":"10.1186\/s40643-023-00721-9"},{"key":"5277_CR8","doi-asserted-by":"publisher","first-page":"102138","DOI":"10.1016\/j.rineng.2024.102138","volume":"22","author":"M Mirsalami","year":"2024","unstructured":"Mirsalami, M., & Mirsalami, M. (2024). Evaluation of mesoporous silica particles as a support for lipase immobilization in biodiesel production: Enhanced ethyl ester synthesis from algal oil. Results in Engineering, 22, 102138. https:\/\/doi.org\/10.1016\/j.rineng.2024.102138","journal-title":"Results in Engineering"},{"key":"5277_CR9","doi-asserted-by":"publisher","first-page":"2566","DOI":"10.1016\/j.biortech.2007.04.042","volume":"99","author":"S Pahujani","year":"2008","unstructured":"Pahujani, S., Kanwar, S. S., Chauhan, G., & Gupta, R. (2008). Glutaraldehyde activation of polymer Nylon-6 for lipase immobilization: Enzyme characteristics and stability. Bioresource Technology, 99, 2566\u20132570. https:\/\/doi.org\/10.1016\/j.biortech.2007.04.042","journal-title":"Bioresource Technology"},{"key":"5277_CR10","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.molcatb.2016.04.006","volume":"130","author":"S Velasco-Lozano","year":"2016","unstructured":"Velasco-Lozano, S., L\u00f3pez-Gallego, F., Rocha-Martins, J., Guis\u00e1n, M., & Favela-Torres, E. (2016). Improving enantioselectivity of lipase from Candida rugosa by carrier-bound and carrier-free immobilization. Journal of Molecular Catalysis B: Enzymatic, 130, 32\u201339. https:\/\/doi.org\/10.1016\/j.molcatb.2016.04.006","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"5277_CR11","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.enconman.2016.09.049","volume":"141","author":"Z Amini","year":"2017","unstructured":"Amini, Z., Ilham, Z., Ong, H. C., Mazaheri, H., & Chen, W.-H. (2017). State of the art and prospective of lipase-catalyzed transesterification reaction for biodiesel production. Energy Conversion and Management, 141, 339\u2013353. https:\/\/doi.org\/10.1016\/j.enconman.2016.09.049","journal-title":"Energy Conversion and Management"},{"issue":"2","key":"5277_CR12","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1021\/acs.chemrev.7b00203","volume":"118","author":"RA Sheldon","year":"2018","unstructured":"Sheldon, R. A., & Woodley, J. M. (2018). Role of biocatalysis in sustainable chemistry. Chemistry Reviews, 118(2), 801\u2013838. https:\/\/doi.org\/10.1021\/acs.chemrev.7b00203","journal-title":"Chemistry Reviews"},{"key":"5277_CR13","doi-asserted-by":"publisher","first-page":"118217","DOI":"10.1016\/j.apcata.2021.118217","volume":"622","author":"TA Costa-Silva","year":"2021","unstructured":"Costa-Silva, T. A., Carvalho, A. K. F., Souza, C. R. F., De Castro, H. F., Bachmann, L., Said, S., & Oliveira, W. P. (2021). Enhancement lipase activity via immobilization onto chitosan beads used as seed particles during fluidized bed drying: Application in butyl butyrate production. Applied Catalysis A: General, 622, 118217. https:\/\/doi.org\/10.1016\/j.apcata.2021.118217","journal-title":"Applied Catalysis A: General"},{"key":"5277_CR14","doi-asserted-by":"crossref","unstructured":"Garcia-Galan C., Berenguer-Murcia A., Fernandez-Lafuente R., Rodrigues R.C. (2011). Potential of different enzyme immobilization strategies to improve enzyme performance. Advanced Synthesis & Catalysis, 353 (16), 2885\u20132904. h10.1002\/adsc.201100534.","DOI":"10.1002\/adsc.201100534"},{"key":"5277_CR15","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1007\/s10973-018-7467-5","volume":"134","author":"GS Padilha","year":"2018","unstructured":"Padilha, G. S., Campos, V. A. B., Costa, M. C., & Franco, T. T. (2018). Multi-walled carbon nanotubes used as support for lipase from Burkholderia cepacia: Morphological and physicochemical properties. Journal of Thermal Analysis and Calorimetry, 134, 1021\u20131029. https:\/\/doi.org\/10.1007\/s10973-018-7467-5","journal-title":"Journal of Thermal Analysis and Calorimetry"},{"issue":"1","key":"5277_CR16","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1080\/00986445.2017.1370707","volume":"205","author":"GS Padilha","year":"2018","unstructured":"Padilha, G. S., Tambourgi, E. B., & Alegre, R. M. (2018). Evaluation of lipase from Burkholderia cepacia immobilized in alginate beads and application in the synthesis of banana flavor (isoamyl acetate). Chemical Engineering Communications, 205(1), 23\u201333. https:\/\/doi.org\/10.1080\/00986445.2017.1370707","journal-title":"Chemical Engineering Communications"},{"key":"5277_CR17","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1007\/s11746-010-1727-2","volume":"88","author":"G Fernandez-Lorente","year":"2011","unstructured":"Fernandez-Lorente, G., Filice, M., Lopez-Vela, D., Pizarro, C., Wilson, L., Betancor, L., Avila, Y., & Guisan, J. M. (2011). Cross-linking of lipases adsorbed on hydrophobic supports: Highly selective hydrolysis of fish oil catalyzed by RML. Journal of the American Oil Chemistry\u2019s Society, 88, 801\u2013807. https:\/\/doi.org\/10.1007\/s11746-010-1727-2","journal-title":"Journal of the American Oil Chemistry\u2019s Society"},{"key":"5277_CR18","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.enzmictec.2017.07.001","volume":"106","author":"H Zaak","year":"2017","unstructured":"Zaak, H., Fernandez-Lopes, L., Otero, C., Sassi, M., & Fernandez-Lafuente, R. (2017). Improved stability of immobilized lipases via modification with polyethylenimine and glutaraldehyde. Enzyme and Microbial Technology, 106, 67\u201374. https:\/\/doi.org\/10.1016\/j.enzmictec.2017.07.001","journal-title":"Enzyme and Microbial Technology"},{"key":"5277_CR19","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1016\/j.procbio.2012.04.019","volume":"47","author":"O Barbosa","year":"2012","unstructured":"Barbosa, O., Torres, R., Ortiz, C., & Fernandez-Lafuente, R. (2012). Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica. Process Biochemistry, 47, 1220\u20131227. https:\/\/doi.org\/10.1016\/j.procbio.2012.04.019","journal-title":"Process Biochemistry"},{"key":"5277_CR20","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.biombioe.2018.09.011","volume":"119","author":"RN Vilas B\u00f4as","year":"2018","unstructured":"Vilas B\u00f4as, R. N., Ceron, A. A., Bento, H. B. S., & De Castro, H. F. (2018). Application of an immobilized Rhizopus oryzae lipase to batch and continuous ester synthesis with a mixture of a lauric acid and fusel oil. Biomass and Bioenergy, 119, 61\u201368. https:\/\/doi.org\/10.1016\/j.biombioe.2018.09.011","journal-title":"Biomass and Bioenergy"},{"key":"5277_CR21","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1007\/s10934-019-00786-y","volume":"27","author":"Y Gu","year":"2020","unstructured":"Gu, Y., Xue, P., & Shi, K. (2020). A novel support of sponge-like cellulose composite polymer for immobilizing laccase and its application in nitrogenous organics biodegradation. Journal of Porous Materials, 27, 73\u201382. https:\/\/doi.org\/10.1007\/s10934-019-00786-y","journal-title":"Journal of Porous Materials"},{"key":"5277_CR22","doi-asserted-by":"publisher","first-page":"2689","DOI":"10.1039\/D3RE00420A","volume":"8","author":"JR Guimar\u00e3es","year":"2023","unstructured":"Guimar\u00e3es, J. R., Oliveira, K. S. G. C., Gon\u00e7alves, M. C. P., Romanelli, J. P., Lopes, L. A., Berenguer-Murcia, A., Fernandez-Lafuente, R., & Tardioli, P. W. (2023). A review of lipase immobilization on hydrophobic supports incorporating systematic mapping principles. Reaction Chemistry and & Engineering, 8, 2689\u20132702. https:\/\/doi.org\/10.1039\/D3RE00420A","journal-title":"Reaction Chemistry and & Engineering"},{"key":"5277_CR23","doi-asserted-by":"publisher","first-page":"109397","DOI":"10.1016\/j.enzmictec.2019.109397","volume":"132","author":"L Dal Magro","year":"2020","unstructured":"Dal Magro, L., Kornecki, J. F., Klein, M. P., Rodrigues, R. C., & Fernandez-Lafuente, R. (2020). Pectin lyase immobilization using the glutaraldehyde chemistry increases the enzyme operation range. Enzyme Microbiology Technology, 132, 109397. https:\/\/doi.org\/10.1016\/j.enzmictec.2019.109397","journal-title":"Enzyme Microbiology Technology"},{"key":"5277_CR24","doi-asserted-by":"publisher","unstructured":"Ishimoto, T., Jigawa, K., Henares, Te. G., Sueyoshi, K., Endo, T., Hisamoto, H. (2014). Efficient immobilization of the enzyme and substrate for a single-step caspase-3 inhibitor assay using a combinable PDMS capillary sensor array. RCS Advances, 4, 7682-7687.https:\/\/doi.org\/10.1039\/C3RA46976J","DOI":"10.1039\/C3RA46976J"},{"key":"5277_CR25","doi-asserted-by":"publisher","first-page":"66","DOI":"10.2174\/1874091X01711010066","volume":"11","author":"RF Perna","year":"2017","unstructured":"Perna, R. F., Tiosso, P. C., Sgobi, L. M., Vieira, A. M. S., Vieira, M. F., Tardioli, P. W., Soares, C. M. F., & Zanin, G. M. (2017). Effects of Triton X-100 and PEG on the catalytic properties and thermal stability of lipase from Candida Rugosa free and immobilized on glyoxyl-agarose. The Open Biochemistry Journal, 11, 66\u201376. https:\/\/doi.org\/10.2174\/1874091X01711010066","journal-title":"The Open Biochemistry Journal"},{"key":"5277_CR26","doi-asserted-by":"publisher","unstructured":"Mesa, M., Perea\u00f1ez, J. A., Preciado, L. M., Bernal, C. (2018). How the Triton X-100 modulates the activity\/stability of the\u00a0Thermomyces lanuginose\u00a0lipase: Insights from experimental and molecular docking approaches. International Journal of Biological Macromolecules, 120 (Part B), 2410\u20132417. https:\/\/doi.org\/10.1016\/j.ijbiomac.2018.09.009","DOI":"10.1016\/j.ijbiomac.2018.09.009"},{"key":"5277_CR27","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.colsurfb.2008.05.003","volume":"66","author":"N Dizge","year":"2008","unstructured":"Dizge, N., Keskinler, B., & Tanriseven, A. (2008). Covalent attachment of microbial lipase onto microporous styrene\u2013divinylbenzene copolymer by means of polyglutaraldehyde. Colloids and Surfaces B: Biointerfaces, 66, 34\u201338. https:\/\/doi.org\/10.1016\/j.colsurfb.2008.05.003","journal-title":"Colloids and Surfaces B: Biointerfaces"},{"key":"5277_CR28","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.molcatb.2010.01.013","volume":"63","author":"\u00d6 Aybast\u0131er","year":"2010","unstructured":"Aybast\u0131er, \u00d6., & Demir, C. (2010). Optimization of immobilization conditions of Thermomyces lanuginosus lipase on styrene-divinylbenzene copolymer using response surface methodology. Journal of Molecular Catalysis B: Enzymatic, 63, 170\u2013178. https:\/\/doi.org\/10.1016\/j.molcatb.2010.01.013","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"issue":"15","key":"5277_CR29","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.jmmm.2016.11.061","volume":"426","author":"BS Bento","year":"2017","unstructured":"Bento, B. S., De Castro, H. F., Oliveira, P. C., & Freitas, L. (2017). Magnetized poly (STY-co-DVB) as a matrix for immobilizing microbial lipase to be used in biotransformation. Journal of Magnetism and Magnetic Materials, 426(15), 95\u2013101. https:\/\/doi.org\/10.1016\/j.jmmm.2016.11.061","journal-title":"Journal of Magnetism and Magnetic Materials"},{"issue":"1","key":"5277_CR30","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.enzmictec.2011.03.002","volume":"49","author":"K Hernandez","year":"2011","unstructured":"Hernandez, K., Garcia-Galan, C., & Fernandez-Lafuente, R. (2011). Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene\u2013divinylbenzene beads. Enzyme and Microbial Technology, 49(1), 72\u201378. https:\/\/doi.org\/10.1016\/j.enzmictec.2011.03.002","journal-title":"Enzyme and Microbial Technology"},{"key":"5277_CR31","doi-asserted-by":"publisher","unstructured":"Pinto, M. C. C., de Souza e Castro, N. L., Cipolatti, E. P., Fernandez-Lafuente, R., Manoel, E. A., Freire, D. M. G., Pinto, J. C. (2019). Effects of reaction operation policies on properties of core\u2013shell polymer supports used for preparation of highly active biocatalysts. Macromolecular Reaction Engineering, 13, 1.https:\/\/doi.org\/10.1002\/mren.201800055","DOI":"10.1002\/mren.201800055"},{"key":"5277_CR32","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford, M. M. (1976). rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analitical Biochemistry, 72, 57\u201362. https:\/\/doi.org\/10.1016\/0003-2697(76)90527-3","journal-title":"Analitical Biochemistry"},{"key":"5277_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.apsusc.2004.05.195","volume":"238","author":"ML Foresti","year":"2004","unstructured":"Foresti, M. L., & Ferreira, M. L. (2004). Ethanol pretreatment effect and particle diameter issues on the adsorption of Candida rugosa lipase onto polypropylene powder. Applied Surface Science, 238, 1\u20134. https:\/\/doi.org\/10.1016\/j.apsusc.2004.05.195","journal-title":"Applied Surface Science"},{"key":"5277_CR34","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.biortech.2013.02.052","volume":"134","author":"AB Martins","year":"2013","unstructured":"Martins, A. B., Friedrich, J. L. R., Cavalheiro, J. C., Garcia-Galan, C., Barbos, O., Ayub, M. A. Z., Fernandez-Lafuente, R., & Rodrigues, R. C. (2013). Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene\u2013divinylbenzene beads. Bioresource Biotechnology, 134, 417\u2013422. https:\/\/doi.org\/10.1016\/j.biortech.2013.02.052","journal-title":"Bioresource Biotechnology"},{"key":"5277_CR35","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.rser.2014.12.021","volume":"44","author":"X Zhao","year":"2015","unstructured":"Zhao, X., Feng, Q., Chongli, Y., Wei, D., & Dehua, L. (2015). Lipase-catalyzed process for biodiesel production: Enzyme immobilization, process simulation and optimization. Renewable and Sustainable Energy Reviews, 44, 182\u2013197. https:\/\/doi.org\/10.1016\/j.rser.2014.12.021","journal-title":"Renewable and Sustainable Energy Reviews"},{"issue":"1","key":"5277_CR36","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.colsurfb.2008.10.021","volume":"69","author":"EY Ozmen","year":"2009","unstructured":"Ozmen, E. Y., & Yilmaz, M. (2009). Pretreatment of Candida rugosa lipase with soybean oil before immobilization on \u03b2-cyclodextrin-based polymer. Colloids and Surfaces B: Biointerfaces, 69(1), 58\u201362. https:\/\/doi.org\/10.1016\/j.colsurfb.2008.10.021","journal-title":"Colloids and Surfaces B: Biointerfaces"},{"issue":"1","key":"5277_CR37","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S1369-703X(99)00061-3","volume":"5","author":"PC De Oliveira","year":"2000","unstructured":"De Oliveira, P. C., Alves, G. M., & De Castro, H. F. (2000). Immobilisation studies and catalytic properties of microbial lipase onto styrene\u2013divinylbenzene copolymer. Biochemical Engineering Journal, 5(1), 63\u201371. https:\/\/doi.org\/10.1016\/S1369-703X(99)00061-3","journal-title":"Biochemical Engineering Journal"},{"key":"5277_CR38","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.ijbiomac.2017.01.038","volume":"97","author":"S Rehman","year":"2017","unstructured":"Rehman, S., Wang, P., Bhatti, H. N., Bilal, M., & Asgher, M. (2017). Improved catalytic properties of Penicillium notatum lipase immobilized in nanoscale silicone polymeric films. International Journal of Biological Macromolecules, 97, 279\u2013286. https:\/\/doi.org\/10.1016\/j.ijbiomac.2017.01.038","journal-title":"International Journal of Biological Macromolecules"},{"issue":"5","key":"5277_CR39","doi-asserted-by":"publisher","first-page":"790","DOI":"10.2144\/04375RV01","volume":"37","author":"I Migneault","year":"2004","unstructured":"Migneault, I., Dartiguenave, C., Bertrand, M. J., & Waldron, K. C. (2004). Glutaraldehyde: Behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking. BioTechniques, 37(5), 790\u2013802. https:\/\/doi.org\/10.2144\/04375RV01","journal-title":"BioTechniques"},{"issue":"3","key":"5277_CR40","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1080\/10242422.2017.1314466","volume":"36","author":"RM Blanco","year":"2017","unstructured":"Blanco, R. M., & Rold\u00e1n, I. (2017). Two additives to improve stability of immobilized lipase. Biocatalysis and Biotransformation, 36(3), 224\u2013232. https:\/\/doi.org\/10.1080\/10242422.2017.1314466","journal-title":"Biocatalysis and Biotransformation"},{"key":"5277_CR41","doi-asserted-by":"publisher","first-page":"103184","DOI":"10.1016\/j.bcab.2024.103184","volume":"58","author":"D Rajan","year":"2024","unstructured":"Rajan, D., Benesh, A., & Nampoothiri, K. M. (2024). Biomanufacturing of ricinoleic acid from castor oil using immobilized lipase of Pseudomonas guariconensis as biocatalyst. Biocatalysis and Agricultural Biotechnology, 58, 103184. https:\/\/doi.org\/10.1016\/j.bcab.2024.103184","journal-title":"Biocatalysis and Agricultural Biotechnology"},{"issue":"7","key":"5277_CR42","doi-asserted-by":"publisher","first-page":"1692","DOI":"10.1016\/j.procbio.2006.02.022","volume":"41","author":"M Karra-Ch\u00e2abouni","year":"2006","unstructured":"Karra-Ch\u00e2abouni, M., Ghamgui, H., Bezzine, S., Rekik, A., & Gargouri, Y. (2006). Production of flavour esters by immobilized Staphylococcus simulans lipase in a solvent-free system. Process Biochemistry, 41(7), 1692\u20131698. https:\/\/doi.org\/10.1016\/j.procbio.2006.02.022","journal-title":"Process Biochemistry"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-025-05277-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12010-025-05277-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-025-05277-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T09:46:50Z","timestamp":1754646410000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12010-025-05277-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,4]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["5277"],"URL":"https:\/\/doi.org\/10.1007\/s12010-025-05277-2","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,4]]},"assertion":[{"value":"2 May 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 June 2025","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}