{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T23:14:24Z","timestamp":1785798864024,"version":"3.56.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1007\/s12010-025-05512-w","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T07:17:36Z","timestamp":1766733456000},"page":"990-1003","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Berbamine Attenuates Chronic Kidney Disease-Associated Pruritus by Targeting MRGPRX1\/MrgprC11 Signaling"],"prefix":"10.1007","volume":"198","author":[{"given":"Mingzheng","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minggang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,26]]},"reference":[{"key":"5512_CR1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13080527","author":"P Agarwal","year":"2021","unstructured":"Agarwal, P., Garg, V., Karagaiah, P., et al. (2021). Chronic kidney disease-associated pruritus. Toxins (Basel). https:\/\/doi.org\/10.3390\/toxins13080527","journal-title":"Toxins (Basel)"},{"key":"5512_CR2","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1159\/000518391","volume":"46","author":"M Makar","year":"2021","unstructured":"Makar, M., Smyth, B., & Brennan, F. (2021). Chronic kidney disease-associated pruritus: A review. Kidney and Blood Pressure Research, 46, 659\u201369. https:\/\/doi.org\/10.1159\/000518391","journal-title":"Kidney and Blood Pressure Research"},{"key":"5512_CR3","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/ki.2013.454","volume":"87","author":"T Mettang","year":"2015","unstructured":"Mettang, T., & Kremer, A. E. (2015). Uremic pruritus. Kidney International, 87, 685\u201391. https:\/\/doi.org\/10.1038\/ki.2013.454","journal-title":"Kidney International"},{"key":"5512_CR4","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1016\/j.ekir.2020.04.027","volume":"5","author":"HA Verduzco","year":"2020","unstructured":"Verduzco, H. A., & Shirazian, S. (2020). CKD-associated pruritus: New insights into diagnosis, pathogenesis, and management. Kidney International Reports, 5, 1387\u2013402. https:\/\/doi.org\/10.1016\/j.ekir.2020.04.027","journal-title":"Kidney International Reports"},{"key":"5512_CR5","doi-asserted-by":"publisher","unstructured":"Elhag, S., Rivas, N., Tejovath, S. Chronic kidney Disease-Associated pruritus: A glance at novel and Lesser-Known Treatments[J]. Cureus 2022;14:e21127. https:\/\/doi.org\/10.7759\/cureus.21127","DOI":"10.7759\/cureus.21127"},{"key":"5512_CR6","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1016\/j.neuron.2015.11.001","volume":"88","author":"P Geppetti","year":"2015","unstructured":"Geppetti, P., Veldhuis, N. A., Lieu, T., et al. (2015). G protein-coupled receptors: Dynamic machines for signaling pain and itch. Neuron, 88, 635\u201349. https:\/\/doi.org\/10.1016\/j.neuron.2015.11.001","journal-title":"Neuron"},{"key":"5512_CR7","doi-asserted-by":"publisher","unstructured":"Tsagareli, M. G., Follansbee, T., Iodi, C. M., et al. (2023). Targeting transient receptor potential (TRP) Channels, Mas-Related G-Protein-Coupled receptors (Mrgprs), and Protease-Activated receptors (PARs) to relieve Itch[J]. Pharmaceuticals (Basel), 16. https:\/\/doi.org\/10.3390\/ph16121707","DOI":"10.3390\/ph16121707"},{"key":"5512_CR8","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1038\/s41586-021-04126-6","volume":"600","author":"C Cao","year":"2021","unstructured":"Cao, C., Kang, H. J., Singh, I., et al. (2021). Structure, function and pharmacology of human itch GPCRs. Nature, 600, 170\u201375. https:\/\/doi.org\/10.1038\/s41586-021-04126-6","journal-title":"Nature"},{"key":"5512_CR9","doi-asserted-by":"publisher","first-page":"2236","DOI":"10.1016\/j.jaci.2020.12.626","volume":"147","author":"X Li","year":"2021","unstructured":"Li, X., Yang, H., Han, Y., et al. (2021). Tick peptides evoke itch by activating MrgprC11\/MRGPRX1 to sensitize TRPV1 in pruriceptors[J]. The Journal of Allergy and Clinical Immunology, 147, 2236\u20132248. https:\/\/doi.org\/10.1016\/j.jaci.2020.12.626","journal-title":"The Journal of Allergy and Clinical Immunology"},{"key":"5512_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/foods8100522","author":"B Salehi","year":"2019","unstructured":"Salehi, B., Selamoglu, Z., Sener, B., et al. (2019). Berberis plants-drifting from farm to food applications, phytotherapy, and phytopharmacology. Foods. https:\/\/doi.org\/10.3390\/foods8100522","journal-title":"Foods"},{"key":"5512_CR11","doi-asserted-by":"publisher","first-page":"201","DOI":"10.2174\/1871520622666220509174306","volume":"23","author":"B Hu","year":"2023","unstructured":"Hu, B., Yang, Y., Tu, J., et al. (2023). Berbamine exerts an anti-oncogenic effect on pancreatic cancer by regulating Wnt and DNA damage-related pathways. Anti-Cancer Agents in Medicinal Chemistry, 23, 201\u201309. https:\/\/doi.org\/10.2174\/1871520622666220509174306","journal-title":"Anti-Cancer Agents in Medicinal Chemistry"},{"key":"5512_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2024.176634","volume":"975","author":"W Tang","year":"2024","unstructured":"Tang, W., Ma, J., Chen, K., et al. (2024). Berbamine ameliorates DSS-induced colitis by inhibiting peptidyl-arginine deiminase 4-dependent neutrophil extracellular traps formation. European Journal of Pharmacology, 975, Article 176634. https:\/\/doi.org\/10.1016\/j.ejphar.2024.176634","journal-title":"European Journal of Pharmacology"},{"key":"5512_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.156619","volume":"140","author":"CH Yao","year":"2025","unstructured":"Yao, C. H., Zhang, Z. M., Zhang, S., et al. (2025). Berbamine alleviates neuropathic pain via suppressing spinal TMEM34\/SGK1\/FOXO3 axis. Phytomedicine, 140, Article 156619. https:\/\/doi.org\/10.1016\/j.phymed.2025.156619","journal-title":"Phytomedicine"},{"key":"5512_CR14","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214321","author":"K Ryu","year":"2022","unstructured":"Ryu, K., Heo, Y., Lee, Y., et al. (2022). Berbamine reduces chloroquine-induced itch in mice through inhibition of MrgprX1. International Journal of Molecular Sciences. https:\/\/doi.org\/10.3390\/ijms232214321","journal-title":"International Journal of Molecular Sciences"},{"key":"5512_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12134505","author":"V Esteve-Simo","year":"2023","unstructured":"Esteve-Simo, V., Perez-Morales, R., Buades-Fuster, J. M., et al. (2023). Chronic kidney disease-associated pruritus and quality of life: Learning from our patients. Journal of Clinical Medicine. https:\/\/doi.org\/10.3390\/jcm12134505","journal-title":"Journal of Clinical Medicine"},{"key":"5512_CR16","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1111\/cod.14051","volume":"86","author":"F Li","year":"2022","unstructured":"Li, F., Wang, C., Hu, D., et al. (2022). mMrgprA3\/mMrgprC11\/hMrgprX1: Potential therapeutic targets for allergic contact dermatitis-induced pruritus in mice and humans. Contact Dermatitis, 86, 286\u201394. https:\/\/doi.org\/10.1111\/cod.14051","journal-title":"Contact Dermatitis"},{"key":"5512_CR17","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.toxicon.2018.09.002","volume":"154","author":"SS Espino","year":"2018","unstructured":"Espino, S. S., Robinson, S. D., Safavi-Hemami, H., et al. (2018). Conopeptides promote itch through human itch receptor hMgprX1. Toxicon, 154, 28\u201334. https:\/\/doi.org\/10.1016\/j.toxicon.2018.09.002","journal-title":"Toxicon"},{"key":"5512_CR18","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1016\/j.jid.2024.05.031","volume":"145","author":"H Yang","year":"2025","unstructured":"Yang, H., Chen, Y., Wang, L., et al. (2025). The fungal secretory peptide Micasin induces itch by activating MRGPRX1\/C11\/A1 on peripheral neurons. The Journal of Investigative Dermatology, 145, 618\u201330. https:\/\/doi.org\/10.1016\/j.jid.2024.05.031","journal-title":"The Journal of Investigative Dermatology"},{"key":"5512_CR19","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010420","author":"O Mahmoud","year":"2022","unstructured":"Mahmoud, O., Soares, G. B., & Yosipovitch, G. (2022). Transient receptor potential channels and itch. International Journal of Molecular Sciences. https:\/\/doi.org\/10.3390\/ijms24010420","journal-title":"International Journal of Molecular Sciences"},{"key":"5512_CR20","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.jpain.2022.09.013","volume":"24","author":"KM Smith","year":"2023","unstructured":"Smith, K. M., Nguyen, E., & Ross, S. E. (2023). The delta-opioid receptor bidirectionally modulates itch. The Journal of Pain, 24, 264\u201372. https:\/\/doi.org\/10.1016\/j.jpain.2022.09.013","journal-title":"The Journal of Pain"},{"key":"5512_CR21","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.iac.2016.08.007","volume":"37","author":"M Kido-Nakahara","year":"2017","unstructured":"Kido-Nakahara, M., Furue, M., Ulzii, D., et al. (2017). Itch in atopic dermatitis. Immunology and Allergy Clinics of North America, 37, 113\u201322. https:\/\/doi.org\/10.1016\/j.iac.2016.08.007","journal-title":"Immunology and Allergy Clinics of North America"},{"key":"5512_CR22","doi-asserted-by":"publisher","first-page":"i23","DOI":"10.1093\/ckj\/sfab200","volume":"14","author":"S Schricker","year":"2021","unstructured":"Schricker, S., & Kimmel, M. (2021). Unravelling the pathophysiology of chronic kidney disease-associated pruritus. Clinical Kidney Journal, 14, i23-31. https:\/\/doi.org\/10.1093\/ckj\/sfab200","journal-title":"Clinical Kidney Journal"},{"key":"5512_CR23","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1159\/000533469","volume":"52","author":"G Ramirez-Guerrero","year":"2023","unstructured":"Ramirez-Guerrero, G., Ronco, C., & Reis, T. (2023). Chronic kidney disease-associated pruritus: Nomenclature and treatment - We need to take two steps forward. Blood Purification, 52, 821\u201323. https:\/\/doi.org\/10.1159\/000533469","journal-title":"Blood Purification"},{"key":"5512_CR24","doi-asserted-by":"publisher","first-page":"17399","DOI":"10.1074\/jbc.M117.787887","volume":"292","author":"VB Reddy","year":"2017","unstructured":"Reddy, V. B., & Lerner, E. A. (2017). Activation of mas-related G-protein-coupled receptors by the house dust mite cysteine protease Der p1 provides a new mechanism linking allergy and inflammation. The Journal of Biological Chemistry, 292, 17399\u2013406. https:\/\/doi.org\/10.1074\/jbc.M117.787887","journal-title":"The Journal of Biological Chemistry"},{"key":"5512_CR25","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1177\/00033197241238512","volume":"76","author":"T He","year":"2025","unstructured":"He, T., Mohammadpour, B., Willman, M., et al. (2025). Prognostic role of neutrophil to lymphocyte ratio in contrast-induced nephropathy: A systematic review and meta-analysis. Angiology, 76, 819\u201332. https:\/\/doi.org\/10.1177\/00033197241238512","journal-title":"Angiology"},{"key":"5512_CR26","doi-asserted-by":"publisher","DOI":"10.36141\/svdld.v42i2.14552","volume":"42","author":"S Khanzadeh","year":"2025","unstructured":"Khanzadeh, S., Aminizadeh, S., Nourigheimasi, S., et al. (2025). The neutrophil-to-lymphocyte ratio as a diagnostic and prognostic biomarker in pulmonary hypertension: A systematic review. Sarcoidosis, Vasculitis, and Diffuse Lung Diseases, 42, Article 14552. https:\/\/doi.org\/10.36141\/svdld.v42i2.14552","journal-title":"Sarcoidosis, Vasculitis, and Diffuse Lung Diseases"},{"key":"5512_CR27","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-025-13463-y","volume":"25","author":"J Xu","year":"2025","unstructured":"Xu, J., Cheng, X., Xu, M., et al. (2025). Berbamine inhibits cell proliferation and invasion by increasing FTO expression in renal cell carcinoma cells. BMC Cancer, 25, Article 987. https:\/\/doi.org\/10.1186\/s12885-025-13463-y","journal-title":"BMC Cancer"},{"key":"5512_CR28","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04181-9","volume":"21","author":"Y Wang","year":"2023","unstructured":"Wang, Y., Wu, M., Chen, D., et al. (2023). SDMA attenuates renal tubulointerstitial fibrosis through inhibition of STAT4. Journal of Translational Medicine, 21, Article 326. https:\/\/doi.org\/10.1186\/s12967-023-04181-9","journal-title":"Journal of Translational Medicine"},{"key":"5512_CR29","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052758","author":"AA Farooqi","year":"2022","unstructured":"Farooqi, A. A., Wen, R., Attar, R., et al. (2022). Regulation of cell-signaling pathways by berbamine in different cancers. International Journal of Molecular Sciences. https:\/\/doi.org\/10.3390\/ijms23052758","journal-title":"International Journal of Molecular Sciences"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-025-05512-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12010-025-05512-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-025-05512-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T06:54:40Z","timestamp":1771397680000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12010-025-05512-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,26]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["5512"],"URL":"https:\/\/doi.org\/10.1007\/s12010-025-05512-w","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,26]]},"assertion":[{"value":"14 August 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was approved by the Ethics Committee of MDKN Biotechnology Co., Lt. All animal experiments should comply with the ARRIVE guidelines. All methods were carried out in accordance with relevant guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"The authors have no relevant financial or non-financial interests to disclose.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}