{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:11:25Z","timestamp":1789704685979,"version":"4.0.1"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"DOI":"10.1007\/s12010-026-05928-y","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:01:15Z","timestamp":1789704075000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nuclear Import of E2F1 Regulated by KPNB1 is Essential for Proliferation and Invasion of Cervical Cancer Cells"],"prefix":"10.1007","author":[{"given":"Mengjie","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Hai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuecai","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"5928_CR1","first-page":"451","volume":"10","author":"BS Chhikara","year":"2023","unstructured":"Chhikara, B. S., & Parang, K. (2023). Global Cancer Statistics 2022: the trends projection analysis. Chemical Biology Letters, 10, 451\u2013451.","journal-title":"Chemical Biology Letters"},{"key":"5928_CR2","doi-asserted-by":"publisher","first-page":"e536","DOI":"10.1016\/S2214-109X(20)30022-X","volume":"8","author":"KM Abbas","year":"2020","unstructured":"Abbas, K. M., van Zandvoort, K., Brisson, M., & Jit, M. (2020). Effects of updated demography, disability weights, and cervical cancer burden on estimates of human papillomavirus vaccination impact at the global, regional, and national levels: a PRIME modelling study. The Lancet Global Health, 8, e536\u2013e544.","journal-title":"The Lancet Global Health"},{"key":"5928_CR3","doi-asserted-by":"publisher","first-page":"9162921","DOI":"10.1155\/2018\/9162921","volume":"2018","author":"P Nuchpramool","year":"2018","unstructured":"Nuchpramool, P., & Hanprasertpong, J. (2018). Preoperative Neutrophil-Lymphocyte Ratio and Platelet\u2010Lymphocyte Ratio Are Not Clinically Useful in Predicting Prognosis in Early Stage Cervical Cancer. Surgery research and practice, 2018, 9162921.","journal-title":"Surgery research and practice"},{"key":"5928_CR4","doi-asserted-by":"crossref","unstructured":"Venkatas, J., & Singh, M. (2020). Cervical cancer: A meta-analysis, therapy and future of nanomedicine. Ecancermedicalscience, 14, 1111.","DOI":"10.3332\/ecancer.2020.1111"},{"key":"5928_CR5","doi-asserted-by":"publisher","first-page":"2404","DOI":"10.1002\/cncr.30667","volume":"123","author":"W Small Jr","year":"2017","unstructured":"SmallJr, W., Bacon, M. A., Bajaj, A., Chuang, L. T., Fisher, B. J., Harkenrider, M. M., et al. (2017). Cervical cancer: a global health crisis. Cancer, 123, 2404\u20132412.","journal-title":"Cancer"},{"key":"5928_CR6","doi-asserted-by":"publisher","first-page":"1562","DOI":"10.1016\/j.bbamcr.2010.10.013","volume":"1813","author":"M Marfori","year":"2011","unstructured":"Marfori, M., Mynott, A., Ellis, J. J., Mehdi, A. M., Saunders, N. F., Curmi, P. M., et al. (2011). Molecular basis for specificity of nuclear import and prediction of nuclear localization. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1813, 1562\u20131577.","journal-title":"Biochimica et Biophysica Acta (BBA)-Molecular Cell Research"},{"key":"5928_CR7","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1038\/nrc1274","volume":"4","author":"TR Kau","year":"2004","unstructured":"Kau, T. R., Way, J. C., & Silver, P. A. (2004). Nuclear transport and cancer: from mechanism to intervention. Nature Reviews Cancer, 4, 106\u2013117.","journal-title":"Nature Reviews Cancer"},{"key":"5928_CR8","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1016\/j.tcb.2004.09.004","volume":"14","author":"N Mosammaparast","year":"2004","unstructured":"Mosammaparast, N., & Pemberton, L. F. (2004). Karyopherins: from nuclear-transport mediators to nuclear-function regulators. Trends in cell biology, 14, 547\u2013556.","journal-title":"Trends in cell biology"},{"key":"5928_CR9","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1038\/nrm2114","volume":"8","author":"M Stewart","year":"2007","unstructured":"Stewart, M. (2007). Molecular mechanism of the nuclear protein import cycle. Nature reviews Molecular cell biology, 8, 195\u2013208.","journal-title":"Nature reviews Molecular cell biology"},{"key":"5928_CR11","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1007\/s13277-015-3839-7","volume":"37","author":"J Zhu","year":"2016","unstructured":"Zhu, J., Wang, Y., Huang, H., Yang, Q., Cai, J., Wang, Q., et al. (2016). Upregulation of KPN\u03b21 in gastric cancer cell promotes tumor cell proliferation and predicts poor prognosis. Tumor Biology, 37, 661\u2013672.","journal-title":"Tumor Biology"},{"key":"5928_CR12","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1038\/nrc2696","volume":"9","author":"HZ Chen","year":"2009","unstructured":"Chen, H. Z., Tsai, S. Y., & Leone, G. (2009). Emerging roles of E2Fs in cancer: an exit from cell cycle control. Nature reviews cancer, 9, 785\u2013797.","journal-title":"Nature reviews cancer"},{"key":"5928_CR13","doi-asserted-by":"publisher","first-page":"581","DOI":"10.2174\/138945009788680392","volume":"10","author":"W Du","year":"2009","unstructured":"Du, W., & Searle, J. S. (2009). The rb pathway and cancer therapeutics. Current drug targets, 10, 581\u2013589.","journal-title":"Current drug targets"},{"key":"5928_CR14","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1101\/gad.864201","volume":"15","author":"H M\u00fcller","year":"2001","unstructured":"M\u00fcller, H., Bracken, A. P., Vernell, R., Moroni, M. C., Christians, F., Grassilli, E., et al. (2001). E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis. Genes & development, 15, 267\u2013285.","journal-title":"Genes & development"},{"key":"5928_CR15","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1016\/j.cellsig.2014.01.008","volume":"26","author":"L Zhan","year":"2014","unstructured":"Zhan, L., Huang, C., Meng, X. M., Song, Y., Wu, X. Q., Miu, C. G., et al. (2014). Promising roles of mammalian E2Fs in hepatocellular carcinoma. Cellular signalling, 26, 1075\u20131081.","journal-title":"Cellular signalling"},{"key":"5928_CR16","doi-asserted-by":"publisher","first-page":"3770","DOI":"10.18632\/oncotarget.3009","volume":"6","author":"NM Teplyuk","year":"2015","unstructured":"Teplyuk, N. M., Uhlmann, E. J., Wong, A. H. K., Karmali, P., Basu, M., Gabriely, G., et al. (2015). MicroRNA-10b inhibition reduces E2F1-mediated transcription and miR-15\/16 activity in glioblastoma. Oncotarget, 6, 3770.","journal-title":"Oncotarget"},{"key":"5928_CR17","doi-asserted-by":"publisher","first-page":"46","DOI":"10.3390\/ph7010046","volume":"7","author":"RB Batchu","year":"2014","unstructured":"Batchu, R. B., Gruzdyn, O. V., Bryant, C. S., Qazi, A. M., Kumar, S., Chamala, S., et al. (2014). Ritonavir-mediated induction of apoptosis in pancreatic cancer occurs via the RB\/E2F-1 and AKT pathways. Pharmaceuticals, 7, 46\u201357.","journal-title":"Pharmaceuticals"},{"key":"5928_CR18","doi-asserted-by":"publisher","first-page":"e73436","DOI":"10.1371\/journal.pone.0073436","volume":"8","author":"X Ma","year":"2013","unstructured":"Ma, X., Gao, Y., Fan, Y., Ni, D., Zhang, Y., Chen, W., et al. (2013). Overexpression of E2F1 promotes tumor malignancy and correlates with TNM stages in clear cell renal cell carcinoma. PloS one, 8, e73436.","journal-title":"PloS one"},{"key":"5928_CR19","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1038\/onc.2015.198","volume":"35","author":"Y Wang","year":"2016","unstructured":"Wang, Y., Deng, O., Feng, Z., Du, Z., Xiong, X., Lai, J., et al. (2016). RNF126 promotes homologous recombination via regulation of E2F1-mediated BRCA1 expression. Oncogene, 35, 1363\u20131372.","journal-title":"Oncogene"},{"key":"5928_CR20","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1093\/jnci\/dji340","volume":"97","author":"MM Alonso","year":"2005","unstructured":"Alonso, M. M., Fueyo, J., Shay, J. W., Aldape, K. D., Jiang, H., Lee, O. H., et al. (2005). Expression of transcription factor E2F1 and telomerase in glioblastomas: mechanistic linkage and prognostic significance. Journal of the National Cancer Institute, 97, 1589\u20131600.","journal-title":"Journal of the National Cancer Institute"},{"key":"5928_CR21","doi-asserted-by":"publisher","first-page":"e1360","DOI":"10.1038\/cddis.2014.326","volume":"5","author":"P Meng","year":"2014","unstructured":"Meng, P., & Ghosh, R. (2014). Transcription addiction: can we garner the Yin and Yang functions of E2F1 for cancer therapy? Cell death & disease, 5, e1360\u2013e1360.","journal-title":"Cell death & disease"},{"key":"5928_CR22","doi-asserted-by":"publisher","first-page":"1581","DOI":"10.1517\/13543780903151806","volume":"18","author":"R Williams","year":"2009","unstructured":"Williams, R. (2009). Discontinued drugs in 2008: oncology drugs. Expert Opinion on Investigational Drugs, 18, 1581\u20131594.","journal-title":"Expert Opinion on Investigational Drugs"},{"key":"5928_CR23","doi-asserted-by":"crossref","unstructured":"Wang, T., Huang, Z., Huang, N., Peng, Y., Gao, M., Wang, X. (2019). Inhibition of KPNB1 inhibits proliferation and promotes apoptosis of chronic myeloid leukemia cells through regulation of E2F1. OncoTargets and therapy: 10455\u201310467.","DOI":"10.2147\/OTT.S210048"},{"key":"5928_CR24","doi-asserted-by":"publisher","first-page":"3875","DOI":"10.7150\/ijbs.62517","volume":"17","author":"H Sun","year":"2021","unstructured":"Sun, H., Ma, H., Zhang, H., & Ji, M. (2021). Up-regulation of MELK by E2F1 promotes the proliferation in cervical cancer cells. International Journal Of Biological Sciences, 17, 3875\u20133888. https:\/\/doi.org\/10.7150\/ijbs.62517","journal-title":"International Journal Of Biological Sciences"},{"key":"5928_CR25","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1096\/fj.11-191585","volume":"26","author":"HV Kuusisto","year":"2012","unstructured":"Kuusisto, H. V., Wagstaff, K. M., Alvisi, G., Roth, D. M., & Jans, D. A. (2012). Global enhancement of nuclear localization-dependent nuclear transport in transformed cells. The FASEB Journal, 26, 1181\u20131193.","journal-title":"The FASEB Journal"},{"key":"5928_CR26","doi-asserted-by":"publisher","first-page":"32833","DOI":"10.18632\/oncotarget.15834","volume":"8","author":"T Stelma","year":"2017","unstructured":"Stelma, T., & Leaner, V. D. (2017). KPNB1-mediated nuclear import is required for motility and inflammatory transcription factor activity in cervical cancer cells. Oncotarget, 8, 32833.","journal-title":"Oncotarget"},{"key":"5928_CR27","doi-asserted-by":"publisher","first-page":"RA12","DOI":"10.12659\/MSM.882192","volume":"18","author":"Y Zhang","year":"2012","unstructured":"Zhang, Y., Chen, L., Yang, S., & Fang, D. (2012). E2F1: a potential negative regulator of hTERT transcription in normal cells upon activation of oncogenic c-Myc. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 18, RA12.","journal-title":"Medical Science Monitor: International Medical Journal of Experimental and Clinical Research"},{"key":"5928_CR28","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nrclinonc.2015.105","volume":"12","author":"SM Crusz","year":"2015","unstructured":"Crusz, S. M., & Balkwill, F. R. (2015). Inflammation and cancer: advances and new agents. Nature reviews Clinical oncology, 12, 584\u2013596.","journal-title":"Nature reviews Clinical oncology"},{"key":"5928_CR29","doi-asserted-by":"publisher","first-page":"e27723","DOI":"10.1371\/journal.pone.0027723","volume":"6","author":"PJ van der Watt","year":"2011","unstructured":"van der Watt, P. J., Ngarande, E., & Leaner, V. D. (2011). Overexpression of Kpn\u03b21 and Kpn\u03b12 importin proteins in cancer derives from deregulated E2F activity. PloS one, 6, e27723.","journal-title":"PloS one"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-026-05928-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12010-026-05928-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-026-05928-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:01:18Z","timestamp":1789704078000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12010-026-05928-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":28,"alternative-id":["5928"],"URL":"https:\/\/doi.org\/10.1007\/s12010-026-05928-y","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]},"assertion":[{"value":"8 September 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 September 2026","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The ethical clearance for this research was obtained from the Institutional Review Board at the Xinxiang Central Hospital, The Fourth Clinical College of Henan Medical University (approval number 2021-146-01[K]).","order":1,"name":"Ethics","label":"Ethics Approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Informed consent, in written form, was provided by all participants upon their inclusion in the study.","order":2,"name":"Ethics","label":"Consent to Participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":3,"name":"Ethics","label":"Clinical Trial Number","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":4,"name":"Ethics","label":"Consent to Publish","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no relevant financial or non-financial interests to disclose.","order":5,"name":"Ethics","label":"Competing Interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}