{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:10:43Z","timestamp":1789704643721,"version":"4.0.1"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["National Natural Science Foundation of China"],"award-info":[{"award-number":["National Natural Science Foundation of China"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["Natural Science Foundation of Zhejiang Province"],"award-info":[{"award-number":["Natural Science Foundation of Zhejiang Province"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Biochem Biotechnol"],"DOI":"10.1007\/s12010-026-05932-2","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T03:49:18Z","timestamp":1789703358000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering Escherichia coli Nissle 1917 for the highly efficient biosynthesis of food-grade ergothioneine"],"prefix":"10.1007","author":[{"given":"Yuwen","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyi","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuiyi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weirui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lehe","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjiang","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"issue":"6","key":"5932_CR1","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.16333\/j.1001-6880.2015.06.031","volume":"27","author":"Q Liu","year":"2015","unstructured":"Liu, Q., Zhang, Y., & Jiang, W. (2015). Biosynthesis and Catabolism of L-Ergothioneine. Natural Product Research and Development, 27(6), 1112\u20131117. https:\/\/doi.org\/10.16333\/j.1001-6880.2015.06.031","journal-title":"Natural Product Research and Development"},{"issue":"4","key":"5932_CR2","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1002\/biof.5520330401","volume":"33","author":"I Laurenza","year":"2008","unstructured":"Laurenza, I., Colognato, R., Migliore, L., Del Prato, S., & Benzi, L. (2008). Modulation of palmitic acid-induced cell death by ergothioneine: Evidence of an anti-inflammatory action. BioFactors, 33(4), 237\u2013247. https:\/\/doi.org\/10.1002\/biof.5520330401","journal-title":"BioFactors"},{"issue":"4","key":"5932_CR3","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1016\/s0006-291x(03)00224-9","volume":"302","author":"I Rahman","year":"2003","unstructured":"Rahman, I., Gilmour, P. S., Jimenez, L. A., Biswas, S. K., Antonicelli, F., & Aruoma, O. I. (2003). Ergothioneine inhibits oxidative stress- and TNF-alpha-induced NF-kappa B activation and interleukin-8 release in alveolar epithelial cells. Biochemical and Biophysical Research Communications, 302(4), 860\u2013864. https:\/\/doi.org\/10.1016\/s0006-291x(03)00224-9","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"1","key":"5932_CR4","doi-asserted-by":"publisher","first-page":"63","DOI":"10.11851\/j.issn.1673-1557.2024.01.018","volume":"50","author":"S Jia","year":"2024","unstructured":"Jia, S., & Dong, B. (2024). Research Progress on Ergotamine Treatment for Cognitive Decline. Journal of Modern Clinical Medicine, 50(1), 63\u201365. https:\/\/doi.org\/10.11851\/j.issn.1673-1557.2024.01.018","journal-title":"Journal of Modern Clinical Medicine"},{"issue":"9","key":"5932_CR5","doi-asserted-by":"publisher","DOI":"10.3390\/foods14091588","volume":"14","author":"H Zhang","year":"2025","unstructured":"Zhang, H., Liu, Z., Wang, Z., Lei, Z., Jia, Y., Chen, W., Shi, R., & Wang, C. (2025). A review of novel antioxidant ergothioneine: Biosynthesis pathways, production, function and food applications. Foods, 14(9), Article 1588. https:\/\/doi.org\/10.3390\/foods14091588","journal-title":"Foods"},{"issue":"10","key":"5932_CR6","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1002\/1873-3468.14310","volume":"596","author":"N D'Onofrio","year":"2022","unstructured":"D\u2019Onofrio, N., Martino, E., Balestrieri, A., Mele, L., Cautela, D., Castaldo, D., & Balestrieri, M. L. (2022). Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3\/MLKL pathway. FEBS Letters, 596(10), 1313\u20131329. https:\/\/doi.org\/10.1002\/1873-3468.14310","journal-title":"FEBS Letters"},{"issue":"1","key":"5932_CR7","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1007\/s12017-020-08620-4","volume":"23","author":"SS Koh","year":"2021","unstructured":"Koh, S. S., Ooi, S. C., Lui, N. M., Qiong, C., Ho, L. T., Cheah, I. K., Halliwell, B., Herr, D. R., & Ong, W. Y. (2021). Effect of ergothioneine on 7-ketocholesterol-induced endothelial injury. NeuroMolecular Medicine, 23(1), 184\u2013198. https:\/\/doi.org\/10.1007\/s12017-020-08620-4","journal-title":"NeuroMolecular Medicine"},{"key":"5932_CR8","doi-asserted-by":"publisher","unstructured":"Lv, X. (2024). Study on the effect and mechanism of ergothioneine on metabolie dysfunction-associated steatotic liver disease (Master\u2019s thesis, Shandong University). https:\/\/doi.org\/10.27272\/d.cnki.gshdu.2024.001234","DOI":"10.27272\/d.cnki.gshdu.2024.001234"},{"issue":"5","key":"5932_CR9","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22729","volume":"35","author":"SA Salama","year":"2021","unstructured":"Salama, S. A., & Omar, H. A. (2021). Modulating NF-\u03baB, MAPK, and PI3K\/AKT signaling by ergothioneine attenuates iron overload-induced hepatocellular injury in rats. Journal of Biochemical and Molecular Toxicology, 35(5), Article e22729. https:\/\/doi.org\/10.1002\/jbt.22729","journal-title":"Journal of Biochemical and Molecular Toxicology"},{"issue":"10","key":"5932_CR10","doi-asserted-by":"publisher","first-page":"2516","DOI":"10.1248\/cpb.25.2516","volume":"25","author":"N Motohashi","year":"1977","unstructured":"Motohashi, N., Mori, I., Sugiura, Y., & Tanaka, H. (1977). Radioprotective effect of ergothioneine on gamma-irradiation of metmyoglobin: Comparison with cysteine on sulfmyoglobin-formation. Chemical & Pharmaceutical Bulletin, 25(10), 2516\u20132523. https:\/\/doi.org\/10.1248\/cpb.25.2516","journal-title":"Chemical & Pharmaceutical Bulletin"},{"issue":"3","key":"5932_CR11","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/1011-1344(93)80026-6","volume":"17","author":"LT Van den Broeke","year":"1993","unstructured":"Van den Broeke, L. T., & van Beyersbergen, G. M. (1993). Thiols as potential UV radiation protectors: An in vitro study. Journal of Photochemistry and Photobiology B Biology, 17(3), 279\u2013286. https:\/\/doi.org\/10.1016\/1011-1344(93)80026-6","journal-title":"Journal of Photochemistry and Photobiology B Biology"},{"issue":"25","key":"5932_CR12","doi-asserted-by":"publisher","first-page":"14264","DOI":"10.1021\/acs.jafc.4c03348","volume":"72","author":"H Zhang","year":"2024","unstructured":"Zhang, H., Zhang, Y., Zhao, M., Zabed, H. M., & Qi, X. (2024). Fermentative production of ergothioneine by exploring novel biosynthetic pathway and remodulating precursor synthesis pathways. Journal of Agricultural and Food Chemistry, 72(25), 14264\u201314273. https:\/\/doi.org\/10.1021\/acs.jafc.4c03348","journal-title":"Journal of Agricultural and Food Chemistry"},{"issue":"1","key":"5932_CR13","doi-asserted-by":"publisher","first-page":"115","DOI":"10.16488\/j.cnki.1005-9873.2021.01.016","volume":"28","author":"L Feng","year":"2021","unstructured":"Feng, L., Zhang, E. H., Li, Y., Zhou, X., Zhao, C., X., & Ren, J. (2021). Research Progress on Extraction, Separation and Detection of Ergothioneine in Edible Fungi. Acta Edulis Fungi, 28(1), 115\u2013123. https:\/\/doi.org\/10.16488\/j.cnki.1005-9873.2021.01.016","journal-title":"Acta Edulis Fungi"},{"issue":"9","key":"5932_CR14","doi-asserted-by":"publisher","first-page":"743","DOI":"10.15952\/j.cnki.cjsc.1005-1511.21274","volume":"30","author":"X Ma","year":"2022","unstructured":"Ma, X., Chen, X., Wu, Z., Yang, Y., Qi, S., Ge, C., Wang, X., & Sui, Q. (2022). Process Research of Natural Antioxidant Ergothioneine. Chinese Journal of Synthetic Chemistry, 30(9), 743\u2013748. https:\/\/doi.org\/10.15952\/j.cnki.cjsc.1005-1511.21274","journal-title":"Chinese Journal of Synthetic Chemistry"},{"issue":"1","key":"5932_CR15","doi-asserted-by":"publisher","first-page":"86","DOI":"10.13560\/j.cnki.biotech.bull.1985.2023-0663","volume":"40","author":"L Li","year":"2024","unstructured":"Li, L., Xu, S., & Jiang, Y. (2024). Research Progress in the Production of Ergothioneine by Biosynthesis. Biotechnology Bulletin, 40(1), 86\u201399. https:\/\/doi.org\/10.13560\/j.cnki.biotech.bull.1985.2023-0663","journal-title":"Biotechnology Bulletin"},{"issue":"4","key":"5932_CR16","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.13345\/j.cjb.210797","volume":"38","author":"Q Liu","year":"2022","unstructured":"Liu, Q., Mao, Y., Liao, X., Luo, J., Ma, H., & Jiang, W. (2022). Recent progress in ergothioneine biosynthesis: A review. Chinese Journal of Biotechnology, 38(4), 1408\u20131420. https:\/\/doi.org\/10.13345\/j.cjb.210797","journal-title":"Chinese Journal of Biotechnology"},{"issue":"19","key":"5932_CR17","doi-asserted-by":"publisher","first-page":"6632","DOI":"10.1021\/ja101721e","volume":"132","author":"FP Seebeck","year":"2010","unstructured":"Seebeck, F. P. (2010). In vitro reconstitution of Mycobacterial ergothioneine biosynthesis. Journal of the American Chemical Society, 132(19), 6632\u20136633. https:\/\/doi.org\/10.1021\/ja101721e","journal-title":"Journal of the American Chemical Society"},{"issue":"20","key":"5932_CR18","doi-asserted-by":"publisher","first-page":"5382","DOI":"10.1021\/ol502596z","volume":"16","author":"W Hu","year":"2014","unstructured":"Hu, W., Song, H., Sae Her, A., Bak, D. W., Naowarojna, N., Elliott, S. J., Qin, L., Chen, X., & Liu, P. (2014). Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway. Organic Letters, 16(20), 5382\u20135385. https:\/\/doi.org\/10.1021\/ol502596z","journal-title":"Organic Letters"},{"issue":"17","key":"5932_CR19","doi-asserted-by":"publisher","first-page":"6906","DOI":"10.1021\/jacs.8b12596","volume":"141","author":"F Leisinger","year":"2019","unstructured":"Leisinger, F., Burn, R., Meury, M., Lukat, P., & Seebeck, F. P. (2019). Structural and mechanistic basis for anaerobic ergothioneine biosynthesis. Journal of the American Chemical Society, 141(17), 6906\u20136914. https:\/\/doi.org\/10.1021\/jacs.8b12596","journal-title":"Journal of the American Chemical Society"},{"issue":"5","key":"5932_CR20","doi-asserted-by":"publisher","first-page":"1516","DOI":"10.1021\/acs.jafc.1c07541","volume":"70","author":"M Kim","year":"2022","unstructured":"Kim, M., Jeong, D. W., Oh, J. W., Jeong, H. J., Ko, Y. J., Park, S. E., & Han, S. O. (2022). Efficient synthesis of food-derived antioxidant L-ergothioneine by engineered Corynebacterium glutamicum. Journal of Agricultural and Food Chemistry, 70(5), 1516\u20131524. https:\/\/doi.org\/10.1021\/acs.jafc.1c07541","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"5932_CR21","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.ymben.2022.01.012","volume":"70","author":"SA van der Hoek","year":"2022","unstructured":"van der Hoek, S. A., Rusn\u00e1k, M., Wang, G., Stanchev, L. D., de F\u00e1tima Alves, L., Jessop-Fabre, M. M., Paramasivan, K., Jacobsen, I. H., Sonnenschein, N., Mart\u00ednez, J. L., Darbani, B., Kell, D. B., & Borodina, I. (2022). Engineering precursor supply for the high-level production of ergothioneine in Saccharomyces cerevisiae. Metabolic Engineering, 70, 129\u2013142. https:\/\/doi.org\/10.1016\/j.ymben.2022.01.012","journal-title":"Metabolic Engineering"},{"issue":"5","key":"5932_CR22","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.1021\/acs.jafc.7b04924","volume":"66","author":"R Osawa","year":"2018","unstructured":"Osawa, R., Kamide, T., Satoh, Y., Kawano, Y., Ohtsu, I., & Dairi, T. (2018). Heterologous and high production of ergothioneine in Escherichia coli. Journal of Agricultural and Food Chemistry, 66(5), 1191\u20131196. https:\/\/doi.org\/10.1021\/acs.jafc.7b04924","journal-title":"Journal of Agricultural and Food Chemistry"},{"issue":"23","key":"5932_CR23","doi-asserted-by":"publisher","first-page":"6390","DOI":"10.1021\/acs.jafc.0c01846","volume":"68","author":"T Kamide","year":"2020","unstructured":"Kamide, T., Takusagawa, S., Tanaka, N., Ogasawara, Y., Kawano, Y., Ohtsu, I., Satoh, Y., & Dairi, T. (2020). High production of ergothioneine in Escherichia coli using the sulfoxide synthase from Methylobacterium strains. Journal of Agricultural and Food Chemistry, 68(23), 6390\u20136394. https:\/\/doi.org\/10.1021\/acs.jafc.0c01846","journal-title":"Journal of Agricultural and Food Chemistry"},{"issue":"2","key":"5932_CR24","doi-asserted-by":"publisher","first-page":"796","DOI":"10.13345\/j.cjb.210166","volume":"38","author":"L Wang","year":"2022","unstructured":"Wang, L., Wang, Y., Li, J., Du, G., & Kang, Z. (2022). Construction and optimization of ergothioneine-producing Escherichia coli. Chinese Journal of Biotechnology, 38(2), 796\u2013806. https:\/\/doi.org\/10.13345\/j.cjb.210166","journal-title":"Chinese Journal of Biotechnology"},{"issue":"1","key":"5932_CR25","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-38382-w","volume":"9","author":"N Tanaka","year":"2019","unstructured":"Tanaka, N., Kawano, Y., Satoh, Y., Dairi, T., & Ohtsu, I. (2019). Gram-scale fermentative production of ergothioneine driven by overproduction of cysteine in Escherichia coli. Scientific Reports, 9(1), Article 1895. https:\/\/doi.org\/10.1038\/s41598-018-38382-w","journal-title":"Scientific Reports"},{"issue":"15","key":"5932_CR26","doi-asserted-by":"publisher","first-page":"9234","DOI":"10.1021\/acs.jafc.5c01267","volume":"73","author":"X Kang","year":"2025","unstructured":"Kang, X., Wang, Y., Liang, Q., & Luo, W. (2025). Enhancing Ergothioneine Production by Combined Protein and Metabolic Engineering Strategies. Journal of Agricultural and Food Chemistry, 73(15), 9234\u20139245. https:\/\/doi.org\/10.1021\/acs.jafc.5c01267","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"5932_CR27","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2025.09.014","author":"J Yan","year":"2025","unstructured":"Yan, J., Chen, Y., Ma, Y., Yang, X., Yao, Y., Zhao, G., & Zhang, Y. (2025). Efficient ergothioneine production through reconstruction of the methyl and sulfur supply systems in Escherichia coli. Trends in Biotechnology. https:\/\/doi.org\/10.1016\/j.tibtech.2025.09.014","journal-title":"Trends in Biotechnology"},{"key":"5932_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2024.103959","volume":"59","author":"M Hu","year":"2024","unstructured":"Hu, M., & Zhang, T. (2024). Metabolic engineering of the probiotic Escherichia coli Nissle 1917 for lacto-N-triose II production. Food Bioscience, 59, Article 103959. https:\/\/doi.org\/10.1016\/j.fbio.2024.103959","journal-title":"Food Bioscience"},{"issue":"1","key":"5932_CR29","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1021\/acs.jafc.2c07859","volume":"71","author":"L Zhang","year":"2023","unstructured":"Zhang, L., Tang, J., Feng, M., & Chen, S. (2023). Engineering methyltransferase and sulfoxide synthase for high-yield production of ergothioneine. Journal of Agricultural and Food Chemistry, 71(1), 671\u2013679. https:\/\/doi.org\/10.1021\/acs.jafc.2c07859","journal-title":"Journal of Agricultural and Food Chemistry"},{"issue":"1","key":"5932_CR30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105539","volume":"300","author":"L Wei","year":"2024","unstructured":"Wei, L., Liu, L., & Gong, W. (2024). Structure of mycobacterial ergothioneine-biosynthesis C-S lyase EgtE. The Journal of Biological Chemistry, 300(1), Article 105539. https:\/\/doi.org\/10.1016\/j.jbc.2023.105539","journal-title":"The Journal of Biological Chemistry"},{"key":"5932_CR31","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.pep.2017.09.013","volume":"142","author":"Y Han","year":"2018","unstructured":"Han, Y., Guo, W., Su, B., Guo, Y., Wang, J., Chu, B., & Yang, G. (2018). High-level expression of soluble recombinant proteins in Escherichia coli using an HE-maltotriose-binding protein fusion tag. Protein Expression and Purification, 142, 25\u201331. https:\/\/doi.org\/10.1016\/j.pep.2017.09.013","journal-title":"Protein Expression and Purification"},{"key":"5932_CR32","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.enzmictec.2015.06.017","volume":"79\u201380","author":"SK Kim","year":"2015","unstructured":"Kim, S. K., Min, W. K., Park, Y. C., & Seo, J. H. (2015). Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II. Enzyme and Microbial Technology, 79\u201380, 49\u201354. https:\/\/doi.org\/10.1016\/j.enzmictec.2015.06.017","journal-title":"Enzyme and Microbial Technology"},{"key":"5932_CR33","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-115","volume":"10","author":"GH Lai","year":"2014","unstructured":"Lai, G. H., Lin, Y. C., Tsai, Y. L., Lien, Y. Y., Lin, M. K., Chen, H. J., Chang, W. T., Tzen, J. T., & Lee, M. S. (2014). High yield production of pigeon circovirus capsid protein in the E. coli by evaluating the key parameters needed for protein expression. BMC Veterinary Research, 10, Article 115. https:\/\/doi.org\/10.1186\/1746-6148-10-115","journal-title":"BMC Veterinary Research"},{"issue":"5","key":"5932_CR34","doi-asserted-by":"publisher","first-page":"2205","DOI":"10.1007\/s00253-018-09595-w","volume":"103","author":"TKM Nguyen","year":"2019","unstructured":"Nguyen, T. K. M., Ki, M. R., Son, R. G., & Pack, S. P. (2019). The NT11, a novel fusion tag for enhancing protein expression in Escherichia coli. Applied Microbiology and Biotechnology, 103(5), 2205\u20132216. https:\/\/doi.org\/10.1007\/s00253-018-09595-w","journal-title":"Applied Microbiology and Biotechnology"},{"issue":"3","key":"5932_CR35","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s00449-018-2050-9","volume":"42","author":"W Ni","year":"2019","unstructured":"Ni, W., Liu, H., Wang, P., Wang, L., Sun, X., Wang, H., Zhao, G., & Zheng, Z. (2019). Evaluation of multiple fused partners on enhancing soluble level of prenyltransferase NovQ in Escherichia coli. Bioprocess and Biosystems Engineering, 42(3), 465\u2013474. https:\/\/doi.org\/10.1007\/s00449-018-2050-9","journal-title":"Bioprocess and Biosystems Engineering"},{"issue":"7","key":"5932_CR36","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00755-16","volume":"199","author":"GT Wong","year":"2017","unstructured":"Wong, G. T., Bonocora, R. P., Schep, A. N., Beeler, S. M., Lee Fong, A. J., Shull, L. M., Batachari, L. E., Dillon, M., Evans, C., Becker, C. J., Bush, E. C., Hardin, J., Wade, J. T., & Stoebel, D. M. (2017). Genome-wide transcriptional response to varying RpoS levels in Escherichia coli K-12. Journal of Bacteriology, 199(7), Article e00755-16. https:\/\/doi.org\/10.1128\/JB.00755-16","journal-title":"Journal of Bacteriology"},{"key":"5932_CR37","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1758449","volume":"16","author":"P Berger","year":"2026","unstructured":"Berger, P., Bosse-Plois, K., P\u00f6lking, W., Loewe, D., Kouzel, I. U., Berger, M., Dobrindt, U., & Mellmann, A. (2026). RpoS impacts global gene expression and carbon source utilization in Escherichia coli O104: H4. Frontiers in Microbiology, 16, Article 1758449. https:\/\/doi.org\/10.3389\/fmicb.2025.1758449","journal-title":"Frontiers in Microbiology"},{"issue":"12","key":"5932_CR38","doi-asserted-by":"publisher","first-page":"e1004872","DOI":"10.1371\/journal.pgen.1004872","volume":"10","author":"G Saxer","year":"2014","unstructured":"Saxer, G., Krepps, M. D., Merkley, E. D., Ansong, C., Deatherage, B. L., Valovska, M. T., Ristic, N., Yeh, P. T., Prakash, V. P., Leiser, O. P., Nakhleh, L., Gibbons, H. S., Kreuzer, H. W., & Shamoo, Y. (2014). Mutations in global regulators lead to metabolic selection during adaptation to complex environments. PLoS Genetics, 10(12), e1004872. https:\/\/doi.org\/10.1371\/journal.pgen.1004872","journal-title":"PLoS Genetics"},{"key":"5932_CR39","doi-asserted-by":"publisher","first-page":"1296","DOI":"10.1111\/j.1365-2958.2007.05601.x","volume":"63","author":"A Typas","year":"2007","unstructured":"Typas, A., Becker, G., & Hengge, R. (2007). The molecular basis of selective promoter activation by the \u03c3S subunit of RNA polymerase. Molecular Microbiology, 63, 1296\u20131306. https:\/\/doi.org\/10.1111\/j.1365-2958.2007.05601.x","journal-title":"Molecular Microbiology"},{"key":"5932_CR40","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.5.6.3","volume":"4","author":"R Hengge","year":"2011","unstructured":"Hengge, R. (2011). Stationary-phase gene regulation in Escherichia coli \u00a7. EcoSal Plus, 4, Article 10,1128. https:\/\/doi.org\/10.1128\/ecosalplus.5.6.3","journal-title":"EcoSal Plus"},{"issue":"6","key":"5932_CR41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0232549","volume":"15","author":"H Qin","year":"2020","unstructured":"Qin, H., Liu, Y., Cao, X., Jiang, J., & Lian, W. (2020). RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens. PLoS One, 15(6), Article e0232549. https:\/\/doi.org\/10.1371\/journal.pone.0232549","journal-title":"PLoS One"},{"issue":"1","key":"5932_CR42","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/s12934-022-01807-3","volume":"21","author":"Z Chen","year":"2022","unstructured":"Chen, Z., He, Y., Wu, X., Wang, L., Dong, Z., & Chen, X. (2022). Toward more efficient ergothioneine production using the fungal ergothioneine biosynthetic pathway. Microbial Cell Factories, 21(1), 76. https:\/\/doi.org\/10.1186\/s12934-022-01807-3","journal-title":"Microbial Cell Factories"},{"issue":"1","key":"5932_CR43","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-020-01447-5","volume":"19","author":"K Fiege","year":"2020","unstructured":"Fiege, K., & Frankenberg-Dinkel, N. (2020). Construction of a new T7 promoter compatible Escherichia coli Nissle 1917 strain for recombinant production of heme-dependent proteins. Microbial Cell Factories, 19(1), Article 190. https:\/\/doi.org\/10.1186\/s12934-020-01447-5","journal-title":"Microbial Cell Factories"},{"issue":"3","key":"5932_CR44","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1128\/MMBR.66.3.373-395.2002","volume":"66","author":"R Hengge","year":"2002","unstructured":"Hengge, R. (2002). Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase. Microbiology and Molecular Biology Reviews: MMBR, 66(3), 373\u2013395. https:\/\/doi.org\/10.1128\/MMBR.66.3.373-395.2002","journal-title":"Microbiology and Molecular Biology Reviews: MMBR"}],"container-title":["Applied Biochemistry and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-026-05932-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12010-026-05932-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12010-026-05932-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T03:49:28Z","timestamp":1789703368000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12010-026-05932-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":44,"alternative-id":["5932"],"URL":"https:\/\/doi.org\/10.1007\/s12010-026-05932-2","relation":{},"ISSN":["0273-2289","1559-0291"],"issn-type":[{"value":"0273-2289","type":"print"},{"value":"1559-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]},"assertion":[{"value":"9 September 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 September 2026","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing Interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}