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The aim of the present study was to experimentally test whether these appliances may contribute to exposure to toxic elements. In the present study, elastomeric ligatures (ELs) were incubated in artificial human saliva for 1 month (a typical period of their use) and the release of 21 metals (Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mg, Mo, Ni, Pb, Rb, Tl, Ti, Sb, Sr, Sn, Zn, U, V) and 2 metalloids (As and Ge) was studied using inductively coupled plasma\u2013mass spectrometry. For comparison, stainless steel ligatures (SLs) were incubated for 1, 3, and 6 months (since sometimes their use is prolonged) under similar conditions. The determined metal levels were compared to the corresponding safety limits for human exposure. During 1 month, the ELs released Cd, Co, Cr, Mn, Ni, and Sn at total mean \u00b1 SD level of 0.31 \u00b1 0.09, 0.98 \u00b1 0.30, 3.96 \u00b1 1.31, 14.7 \u00b1 8.5, 13.8 \u00b1 4.8, and 49.5 \u00b1 27.7 \u03bcg, respectively. Other elements were always below the detection limits. In case of SL, the release of Co, Cr, Fe, Ni, Mn, and Sn was observed, and the determined values increased over the studied period. After 6 months, their total mean \u00b1 SD levels amounted to 28.6 \u00b1 0.2, 21.7 \u00b1 0.2, 623.5 \u00b1 3.0, 1152.7 \u00b1 1.8, 5.5 \u00b1 0.3, and 22.6 \u00b1 0.2 \u03bcg, respectively. The released metal levels from both ligature types were always below safety limits. The release of Ni from SL during 6 months would constitute 5.0 and 11.5% of tolerable intake in adults and children, respectively. The results of this in vitro study highlight that the use of ligatures in orthodontic treatment can be considered safe in terms of metal exposure although elastic ligatures replaced on a monthly basis appear to be advantageous in comparison to the prolonged use of stainless steel appliances.<\/jats:p>","DOI":"10.1007\/s12011-019-01936-8","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T14:04:32Z","timestamp":1572876272000},"page":"646-653","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Metals and Metalloids Release from Orthodontic Elastomeric and Stainless Steel Ligatures: In Vitro Risk Assessment of Human Exposure"],"prefix":"10.1007","volume":"196","author":[{"given":"Aneta","family":"Olszewska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anetta","family":"Han\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danuta","family":"Bara\u0142kiewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4713-0801","authenticated-orcid":false,"given":"Piotr","family":"Rzymski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,4]]},"reference":[{"key":"1936_CR1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jtemb.2005.10.002","volume":"20","author":"I Iavicoli","year":"2006","unstructured":"Iavicoli I, Falcone G, Alessandrelli M, Cresti R, De Santis V, Salvatori S, Alimonti A, Carelli G (2006) The release of metals from metal-on-metal surface arthroplasty of the hip. 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