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The evaluation of requirements of trace elements, in fact, may be useful to prevent the perturbation of tissue-specific metabolic impair. In particular, essential trace elements in the diet play key roles within sub-cellular metabolic patterns with macro effects at the systemic level, like blood cell stability and semen quality. This effect was studied in breeding stallions, in which semen quality and haemogram are important for reproduction. A case-control feeding trial involved 40 stallions (age: 8\u201321\u00a0years; body weight, BW: 510\u2013531\u00a0kg) of one stud centre, allotted to two experimental groups (<jats:italic>n<\/jats:italic>\u2009=\u200920 control, CON vs.<jats:italic>n<\/jats:italic>\u00a0=\u200920 supplemented, SUPPL100), following a matched-pairs approach based on age. Supplemented stallions (SUPPL100) received a mixed mineral and vitamin supplement of Zn\/Se and \u03b1-tocopherol (\u03b1-TOH) (100\u00a0g\/day stallion) to compound feed, fed as control diet to horses of the control group (CON). Horses resulted deficient in circulating \u03b1-TOH and Zn at the start, though clinically healthy. After supplementation, different plasmatic levels of \u03b1-TOH, Zn and Se were found between groups. Circulating basophils (BASO) and mean cell haemoglobin concentration (MCHC) were affected by the dietary treatment (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Plasmatic Se affected monocyte count, haematocrit, mean cell volume and mean cell haemoglobin concentration. Semen traits were not affected by the dietary treatment per se, except for mobile\/progressive sperm cells (%) of stallions aged &gt;\u200913\u00a0years marginal circulating levels of \u03b1-TOH (<jats:italic>p<\/jats:italic>\u2009=\u20090.04). Ameliorating the micromineral status showed to improve the haemogram of stallions in view of circulating levels of Cu. Semen quality appeared to be strongly dependent on animal effects.<\/jats:p>","DOI":"10.1007\/s12011-020-02447-7","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T21:02:24Z","timestamp":1603486944000},"page":"3287-3296","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Dietary Zn\/Se and \u03b1-Tocopherol Supplementation on Metabolic Milieu, Haemogram and Semen Traits of Breeding Stallions"],"prefix":"10.1007","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8863-9834","authenticated-orcid":false,"given":"Maria Grazia","family":"Cappai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Taras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignazio","family":"Cossu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaele","family":"Cherchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corrado","family":"Dimauro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Accioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianpiero","family":"Boatto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Deroma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Spanu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Gatta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Dall\u2019Aglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Pinna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"2447_CR1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s12550-016-0253-y","volume":"32","author":"S Aboling","year":"2016","unstructured":"Aboling S, Drotleff AM, Cappai MG, Kamphues J (2016) Contamination with ergot bodies (Claviceps purpurea sensu lato) of two horse pastures in Northern Germany. 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