{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:11:23Z","timestamp":1790975483279,"version":"4.1.0"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T00:00:00Z","timestamp":1707350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T00:00:00Z","timestamp":1707350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Animine"},{"DOI":"10.13039\/100009613","name":"Universit\u00e4t Hohenheim","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009613","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biol Trace Elem Res"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>The objective of this study was to determine how different sources of Zn, Mn, and Cu in the feed without and with phytase affect prececal <jats:italic>myo<\/jats:italic>-inositol hexakisphosphate (InsP<jats:sub>6<\/jats:sub>) breakdown to <jats:italic>myo<\/jats:italic>-inositol (MI), prececal P digestibility, bone mineralization, and expression of mineral transporters in the jejunum of broiler chickens. A total of 896 male broiler chicks (Cobb 500) were distributed to 7 diets with 8 replicate pens (16 birds per floor pen). Experimental diets were fed from day 0 to 28. Diets were without or with phytase supplementation (0 or 750 FTU\/kg) and were supplemented with three different trace mineral sources (TMS: sulfates, oxides, or chelates) containing 100\u00a0mg\/kg Zn, 100\u00a0mg\/kg Mn, and 125\u00a0mg\/kg Cu. Prececal InsP<jats:sub>6<\/jats:sub> disappearance and P digestibility were affected by interaction (phytase\u2009\u00d7\u2009TMS: <jats:italic>P<\/jats:italic>\u2009\u2264\u20090.010). In diets without phytase supplementation, prececal InsP<jats:sub>6<\/jats:sub> disappearance and P digestibility were greater (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.001) in birds fed chelated minerals than in birds fed sulfates or oxides. However, no differences were observed between TMS in diets with phytase supplementation. Ileal MI concentration was increased by exogenous phytase but differed depending on TMS (phytase\u2009\u00d7\u2009TMS: <jats:italic>P<\/jats:italic>\u2009\u2264\u20090.050). Tibia ash concentration as well as Zn and Mn concentration in tibia ash were increased by phytase supplementation (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.010), but the Cu concentration in tibia ash was not (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.050). Gene expression of the assayed mineral transporters in the jejunum was not affected by diet (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.050), except for <jats:italic>Zn transporter 5<\/jats:italic> (phytase\u2009\u00d7\u2009TMS: <jats:italic>P<\/jats:italic>\u2009=\u20090.024). In conclusion, the tested TMS had minor effects on endogenous phytate degradation in the digestive tract of broiler chickens. However, in phytase-supplemented diets, the choice of TMS was not relevant to phytate degradation under the conditions of this study.<\/jats:p>","DOI":"10.1007\/s12011-024-04076-w","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T15:02:14Z","timestamp":1707404534000},"page":"5235-5250","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Impact of Trace Mineral Source and Phytase Supplementation on Prececal Phytate Degradation and Mineral Digestibility, Bone Mineralization, and Tissue Gene Expression in Broiler Chickens"],"prefix":"10.1007","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3017-1786","authenticated-orcid":false,"given":"Hanna","family":"Philippi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7832-9656","authenticated-orcid":false,"given":"Vera","family":"Sommerfeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1914-1885","authenticated-orcid":false,"given":"Alessandra","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3156-7889","authenticated-orcid":false,"given":"Markus","family":"Rodehutscord","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9137-6745","authenticated-orcid":false,"given":"Oluyinka A.","family":"Olukosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,8]]},"reference":[{"key":"4076_CR1","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.3382\/ps.0401584","volume":"40","author":"TR Zeigler","year":"1961","unstructured":"Zeigler TR, Leach RM, Norris LC, Scott ML (1961) Zinc requirement of the chick: factors affecting requirement. Poult Sci 40:1584\u20131593. https:\/\/doi.org\/10.3382\/ps.0401584","journal-title":"Poult Sci"},{"key":"4076_CR2","doi-asserted-by":"publisher","DOI":"10.1079\/9781789240924.0000","volume-title":"Mineral nutrition of livestock","author":"NF Suttle","year":"2022","unstructured":"Suttle NF (2022) Mineral nutrition of livestock, 5th edn. CABI, Wallingford","edition":"5"},{"key":"4076_CR3","volume-title":"Nutrient requirements of poultry","author":"National Research Council","year":"1994","unstructured":"National Research Council (1994) Nutrient requirements of poultry, 9th edn. National Academies Press, Washington, DC","edition":"9"},{"key":"4076_CR4","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.anifeedsci.2018.01.014","volume":"237","author":"M Hamdi","year":"2018","unstructured":"Hamdi M, Sol\u00e0 D, Franco R, Durosoy S, Rom\u00e9o A, P\u00e9rez JF (2018) Including copper sulphate or dicopper oxide in the diet of broiler chickens affects performance and copper content in the liver. Anim Feed Sci Technol 237:89\u201397. https:\/\/doi.org\/10.1016\/j.anifeedsci.2018.01.014","journal-title":"Anim Feed Sci Technol"},{"key":"4076_CR5","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1079\/9781789241563.0008","volume-title":"Enzymes in farm animal nutrition","author":"M Rodehutscord","year":"2022","unstructured":"Rodehutscord M, Sommerfeld V, K\u00fchn I, Bedford MR (2022) Phytases Potential and limits of phytate destruction in the digestive tract of pigs and poultry. In: Bedford et al (eds) Enzymes in farm animal nutrition, 3rd edn. CABI, Wallingford, pp 124\u2013152","edition":"3"},{"key":"4076_CR6","doi-asserted-by":"publisher","first-page":"15","DOI":"10.3920\/978-90-8686-836-0_1","volume-title":"Phytate destruction-consequences for precision animal nutrition","author":"M Rodehutscord","year":"2016","unstructured":"Rodehutscord M, Rosenfelder P (2016) Update on phytate degradation pattern in the gastrointestinal tract of pigs and broiler chickens. In: Walk et al (eds) Phytate destruction-consequences for precision animal nutrition. Wageningen Academic Publishers, Wageningen, pp 15\u201328"},{"key":"4076_CR7","doi-asserted-by":"publisher","first-page":"4687","DOI":"10.1021\/jf034122x","volume":"51","author":"NM Tamim","year":"2003","unstructured":"Tamim NM, Angel R (2003) Phytate phosphorus hydrolysis as influenced by dietary calcium and micro-mineral source in broiler diets. J Agric Food Chem 51:4687\u20134693. https:\/\/doi.org\/10.1021\/jf034122x","journal-title":"J Agric Food Chem"},{"key":"4076_CR8","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1007\/s12011-014-0161-y","volume":"163","author":"T Santos","year":"2015","unstructured":"Santos T, Connolly C, Murphy R (2015) Trace element inhibition of phytase activity. Biol Trace Elem Res 163:255\u2013265. https:\/\/doi.org\/10.1007\/s12011-014-0161-y","journal-title":"Biol Trace Elem Res"},{"key":"4076_CR9","doi-asserted-by":"publisher","first-page":"391","DOI":"10.3181\/00379727-115-28922","volume":"115","author":"VT Maddaiah","year":"1964","unstructured":"Maddaiah VT, Kurnick AA, Reid BL (1964) Phytic acid studies. Proc Soc Exp Biol Med 115:391\u2013393. https:\/\/doi.org\/10.3181\/00379727-115-28922","journal-title":"Proc Soc Exp Biol Med"},{"key":"4076_CR10","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/0162-0134(87)80041-7","volume":"30","author":"E Champagne","year":"1987","unstructured":"Champagne E, Hinojosa O (1987) Independent and mutual interactions of copper(II) and zinc(II) ions with phytic acid. J Inorg Biochem 30:15\u201333. https:\/\/doi.org\/10.1016\/0162-0134(87)80041-7","journal-title":"J Inorg Biochem"},{"key":"4076_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0065-2628(08)60110-X","volume":"28","author":"NR Reddy","year":"1982","unstructured":"Reddy NR, Sathe SK, Salunkhe DK (1982) Phytates in legumes and cereals. Adv Food Res 28:1\u201392. https:\/\/doi.org\/10.1016\/S0065-2628(08)60110-X","journal-title":"Adv Food Res"},{"key":"4076_CR12","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1080\/00071668.2021.1940862","volume":"62","author":"D Cardoso","year":"2021","unstructured":"Cardoso D, Romeo A, Durosoy S, Meme N, Chevalier Y, Narcy A (2021) Effect of the physicochemical properties of zinc oxide sources on their bioavailability in broilers. Br Poult Sci 62:846\u2013851. https:\/\/doi.org\/10.1080\/00071668.2021.1940862","journal-title":"Br Poult Sci"},{"key":"4076_CR13","unstructured":"Cobb (2022) Cobb500 Broiler. Performance & nutrition supplement.\u00a0https:\/\/www.cobb-vantress.com\/assets\/5a88f2e793\/Broiler-Performance-Nutrition-Supplement.pdf. Accessed 2022-04-20"},{"key":"4076_CR14","volume-title":"Official methods of analysis of AOAC international","author":"AOAC","year":"2006","unstructured":"AOAC (2006) Official methods of analysis of AOAC international, 18th edn. AOAC International, Arlington, VA","edition":"18"},{"key":"4076_CR15","volume-title":"Revision 4.4, EMMC Version 1994","author":"USEPA","year":"1994","unstructured":"USEPA (1994) Method 200.7: Determination of metals and trace elements in water and wastes by inductively coupled plasma-atomic emission spectrometry. Revision 4.4, EMMC Version 1994. Revision 4.4, EMMC Version 1994. United States Environmental Protection Agency, Cincinnati, OH"},{"key":"4076_CR16","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/0377-8401(95)00916-7","volume":"59","author":"FJ Short","year":"1996","unstructured":"Short FJ, Gorton P, Wiseman J, Boorman KN (1996) Determination of titanium dioxide added as an inert marker in chicken digestibility studies. Anim Feed Sci Technol 59:215\u2013221. https:\/\/doi.org\/10.1016\/0377-8401(95)00916-7","journal-title":"Anim Feed Sci Technol"},{"key":"4076_CR17","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2014.62","volume":"4","author":"E Zeller","year":"2015","unstructured":"Zeller E, Schollenberger M, K\u00fchn I, Rodehutscord M (2015) Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers. J Nutr Sci 4:e1. https:\/\/doi.org\/10.1017\/jns.2014.62","journal-title":"J Nutr Sci"},{"key":"4076_CR18","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.3382\/ps\/pex404","volume":"97","author":"V Sommerfeld","year":"2018","unstructured":"Sommerfeld V, Schollenberger M, K\u00fchn I, Rodehutscord M (2018) Interactive effects of phosphorus, calcium, and phytase supplements on products of phytate degradation in the digestive tract of broiler chickens. Poult Sci 97:1177\u20131188. https:\/\/doi.org\/10.3382\/ps\/pex404","journal-title":"Poult Sci"},{"key":"4076_CR19","doi-asserted-by":"publisher","first-page":"920","DOI":"10.3382\/ps\/pex390","volume":"97","author":"V Sommerfeld","year":"2018","unstructured":"Sommerfeld V, K\u00fcnzel S, Schollenberger M, K\u00fchn I, Rodehutscord M (2018) Influence of phytase or myo-inositol supplements on performance and phytate degradation products in the crop, ileum, and blood of broiler chickens. Poult Sci 97:920\u2013929. https:\/\/doi.org\/10.3382\/ps\/pex390","journal-title":"Poult Sci"},{"key":"4076_CR20","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212 \u0394\u0394CT method. Methods 25:402\u2013408. https:\/\/doi.org\/10.1006\/meth.2001.1262","journal-title":"Methods"},{"key":"4076_CR21","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1080\/00071660410001715867","volume":"45","author":"AL Burrell","year":"2004","unstructured":"Burrell AL, Dozier WA, Davis AJ, Compton MM, Freeman ME, Vendrell PF, Ward TL (2004) Responses of broilers to dietary zinc concentrations and sources in relation to environmental implications. Br Poult Sci 45:255\u2013263. https:\/\/doi.org\/10.1080\/00071660410001715867","journal-title":"Br Poult Sci"},{"key":"4076_CR22","doi-asserted-by":"publisher","first-page":"317","DOI":"10.22358\/jafs\/67987\/2001","volume":"10","author":"S \u015awi\u0105tkiewicz","year":"2001","unstructured":"\u015awi\u0105tkiewicz S, Koreleski J, Zhong D (2001) The bioavailability of zinc from inorganic and organic sources in broiler chickens as affected by addition of phytase. J Anim Feed Sci 10:317\u201328. https:\/\/doi.org\/10.22358\/jafs\/67987\/2001","journal-title":"J Anim Feed Sci"},{"key":"4076_CR23","doi-asserted-by":"publisher","first-page":"178","DOI":"10.2527\/1992.701178x","volume":"70","author":"KJ Wedekind","year":"1992","unstructured":"Wedekind KJ, Hortin AE, Baker DH (1992) Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide. J Anim Sci 70:178\u2013187. https:\/\/doi.org\/10.2527\/1992.701178x","journal-title":"J Anim Sci"},{"key":"4076_CR24","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1017\/S1751731109990978","volume":"4","author":"P Schlegel","year":"2010","unstructured":"Schlegel P, Nys Y, Jondreville C (2010) Zinc availability and digestive zinc solubility in piglets and broilers fed diets varying in their phytate contents, phytase activity and supplemented zinc source. Animal 4:200\u2013209. https:\/\/doi.org\/10.1017\/S1751731109990978","journal-title":"Animal"},{"key":"4076_CR25","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1017\/S1751731112001000","volume":"7","author":"P Schlegel","year":"2013","unstructured":"Schlegel P, Sauvant D, Jondreville C (2013) Bioavailability of zinc sources and their interaction with phytates in broilers and piglets. Animal 7:47\u201359. https:\/\/doi.org\/10.1017\/S1751731112001000","journal-title":"Animal"},{"key":"4076_CR26","doi-asserted-by":"publisher","first-page":"1730","DOI":"10.3390\/ani11061730","volume":"11","author":"L Byrne","year":"2021","unstructured":"Byrne L, Hynes MJ, Connolly CD, Murphy RA (2021) Influence of the chelation process on the stability of organic trace mineral supplements used in animal nutrition. Animals 11:1730. https:\/\/doi.org\/10.3390\/ani11061730","journal-title":"Animals"},{"key":"4076_CR27","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1111\/j.1365-2621.1987.tb06634.x","volume":"52","author":"EJ Fordyce","year":"1987","unstructured":"Fordyce EJ, Forbes RM, Robbins KR, Erdman JW (1987) Phytate\u00d7calcium\/zinc molar ratios: are they predictive of zinc bioavailability? J Food Sci 52:440\u2013444. https:\/\/doi.org\/10.1111\/j.1365-2621.1987.tb06634.x","journal-title":"J Food Sci"},{"key":"4076_CR28","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1002\/jsfa.2740400110","volume":"40","author":"KB Nolan","year":"1987","unstructured":"Nolan KB, Duffin PA, McWeeny DJ (1987) Effects of phytate on mineral bioavailability.in vitro studies on Mg2+, Ca2+, Fe3+, Cu2+ and Zn2+ (also Cd2+) solubilities in the presence of phytate. J Sci Food Agric 40:79\u201385. https:\/\/doi.org\/10.1002\/jsfa.2740400110","journal-title":"J Sci Food Agric"},{"key":"4076_CR29","doi-asserted-by":"publisher","first-page":"3194","DOI":"10.1021\/jf971055w","volume":"46","author":"H Persson","year":"1998","unstructured":"Persson H, T\u00fcrk M, Nyman M, Sandberg AS (1998) Binding of Cu2+, Zn2+, and Cd2+ to inositol tri-, tetra-, penta-, and hexaphosphates. J Agric Food Chem 46:3194\u20133200. https:\/\/doi.org\/10.1021\/jf971055w","journal-title":"J Agric Food Chem"},{"key":"4076_CR30","doi-asserted-by":"publisher","first-page":"3884","DOI":"10.3382\/ps\/pez106","volume":"98","author":"CL Walk","year":"2019","unstructured":"Walk CL, Olukosi OA (2019) Influence of graded concentrations of phytase in high-phytate diets on growth performance, apparent ileal amino acid digestibility, and phytate concentration in broilers from hatch to 28 D post-hatch. Poult Sci 98:3884\u20133893. https:\/\/doi.org\/10.3382\/ps\/pez106","journal-title":"Poult Sci"},{"key":"4076_CR31","doi-asserted-by":"publisher","first-page":"102457","DOI":"10.1016\/j.psj.2022.102457","volume":"102","author":"M Novotny","year":"2023","unstructured":"Novotny M, Sommerfeld V, Krieg J, K\u00fchn I, Huber K, Rodehutscord M (2023) Comparison of mucosal phosphatase activity, phytate degradation, and nutrient digestibility in 3-week-old turkeys and broilers at different dietary levels of phosphorus and phytase. Poult Sci 102:102457. https:\/\/doi.org\/10.1016\/j.psj.2022.102457","journal-title":"Poult Sci"},{"key":"4076_CR32","doi-asserted-by":"publisher","first-page":"1437S","DOI":"10.1093\/jn\/130.5.1437S","volume":"130","author":"KA McCall","year":"2000","unstructured":"McCall KA, Huang C-c, Fierke CA (2000) Function and mechanism of zinc metalloenzymes. J Nutr 130:1437S-1446S. https:\/\/doi.org\/10.1093\/jn\/130.5.1437S","journal-title":"J Nutr"},{"key":"4076_CR33","doi-asserted-by":"publisher","first-page":"103160","DOI":"10.1016\/j.psj.2023.103160","volume":"102","author":"H Philippi","year":"2023","unstructured":"Philippi H, Sommerfeld V, Olukosi OA, Windisch W, Monteiro A, Rodehutscord M (2023) Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens. Poult Sci 102:103160. https:\/\/doi.org\/10.1016\/j.psj.2023.103160","journal-title":"Poult Sci"},{"key":"4076_CR34","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1016\/j.psj.2019.10.014","volume":"99","author":"F Gonzalez-Uarquin","year":"2020","unstructured":"Gonzalez-Uarquin F, Rodehutscord M, Huber K (2020) Myo-inositol: its metabolism and potential implications for poultry nutrition-a review. Poult Sci 99:893\u2013905. https:\/\/doi.org\/10.1016\/j.psj.2019.10.014","journal-title":"Poult Sci"},{"key":"4076_CR35","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.3382\/ps\/pev065","volume":"94","author":"K Huber","year":"2015","unstructured":"Huber K, Zeller E, Rodehutscord M (2015) Modulation of small intestinal phosphate transporter by dietary supplements of mineral phosphorus and phytase in broilers. Poult Sci 94:1009\u20131017. https:\/\/doi.org\/10.3382\/ps\/pev065","journal-title":"Poult Sci"},{"key":"4076_CR36","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1007\/s12011-015-0313-8","volume":"167","author":"E Yenice","year":"2015","unstructured":"Yenice E, M\u0131zrak C, G\u00fcltekin M, Atik Z, Tunca M (2015) Effects of organic and inorganic forms of manganese, zinc, copper, and chromium on bioavailability of these minerals and calcium in late-phase laying hens. Biol Trace Elem Res 167:300\u2013307. https:\/\/doi.org\/10.1007\/s12011-015-0313-8","journal-title":"Biol Trace Elem Res"},{"key":"4076_CR37","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/S0955-2863(00)00125-X","volume":"12","author":"RFP Bertolo","year":"2001","unstructured":"Bertolo RFP, Bettger WJ, Atkinson SA (2001) Calcium competes with zinc for a channel mechanism on the brush border membrane of piglet intestine. J Nutr Biochem 12:66\u201372. https:\/\/doi.org\/10.1016\/S0955-2863(00)00125-X","journal-title":"J Nutr Biochem"},{"key":"4076_CR38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s42523-019-0006-2","volume":"1","author":"S K\u00fcnzel","year":"2019","unstructured":"K\u00fcnzel S, Borda-Molina D, Kraft R, Sommerfeld V, K\u00fchn I, Camarinha-Silva A, Rodehutscord M (2019) Impact of coccidiostat and phytase supplementation on gut microbiota composition and phytate degradation in broiler chickens. Animal microbiome 1:1\u201314. https:\/\/doi.org\/10.1186\/s42523-019-0006-2","journal-title":"Animal microbiome"},{"key":"4076_CR39","doi-asserted-by":"publisher","first-page":"101133","DOI":"10.1016\/j.psj.2021.101133","volume":"100","author":"S K\u00fcnzel","year":"2021","unstructured":"K\u00fcnzel S, Borda-Molina D, Zuber T, Hartung J, Siegert W, Feuerstein D, Camarinha-Silva A, Rodehutscord M (2021) Relative phytase efficacy values as affected by response traits, including ileal microbiota composition. Poult Sci 100:101133. https:\/\/doi.org\/10.1016\/j.psj.2021.101133","journal-title":"Poult Sci"},{"key":"4076_CR40","doi-asserted-by":"publisher","first-page":"1732","DOI":"10.2527\/2004.8261732x","volume":"82","author":"NR Augspurger","year":"2004","unstructured":"Augspurger NR, Spencer JD, Webel DM, Baker DH (2004) Pharmacological zinc levels reduce the phosphorus-releasing efficacy of phytase in young pigs and chickens. J Anim Sci 82:1732\u20131739. https:\/\/doi.org\/10.2527\/2004.8261732x","journal-title":"J Anim Sci"},{"key":"4076_CR41","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/S0377-8401(98)00254-5","volume":"77","author":"C Mohanna","year":"1999","unstructured":"Mohanna C, Nys Y (1999) Changes in zinc and manganese availability in broiler chicks induced by vegetal and microbial phytases. Anim Feed Sci Technol 77:241\u2013253. https:\/\/doi.org\/10.1016\/S0377-8401(98)00254-5","journal-title":"Anim Feed Sci Technol"},{"key":"4076_CR42","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.livsci.2016.07.014","volume":"191","author":"A Ghosh","year":"2016","unstructured":"Ghosh A, Mandal GP, Roy A, Patra AK (2016) Effects of supplementation of manganese with or without phytase on growth performance, carcass traits, muscle and tibia composition, and immunity in broiler chickens. Livest Sci 191:80\u201385. https:\/\/doi.org\/10.1016\/j.livsci.2016.07.014","journal-title":"Livest Sci"},{"key":"4076_CR43","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1093\/ps\/83.8.1335","volume":"83","author":"KM Banks","year":"2004","unstructured":"Banks KM, Thompson KL, Jaynes P, Applegate TJ (2004) The effects of copper on the efficacy of phytase, growth, and phosphorus retention in broiler chicks. Poult Sci 83:1335\u20131341. https:\/\/doi.org\/10.1093\/ps\/83.8.1335","journal-title":"Poult Sci"},{"key":"4076_CR44","doi-asserted-by":"publisher","first-page":"7333","DOI":"10.1002\/jsfa.12879","volume":"103","author":"H Philippi","year":"2023","unstructured":"Philippi H, Sommerfeld V, Windisch W, Olukosi OA, Monteiro A, Rodehutscord M (2023) Interactions of zinc with phytate and phytase in the digestive tract of poultry and pigs: a review. J Sci Food Agric 103:7333\u20137342. https:\/\/doi.org\/10.1002\/jsfa.12879","journal-title":"J Sci Food Agric"},{"key":"4076_CR45","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1093\/ps\/86.3.531","volume":"86","author":"Y Pang","year":"2007","unstructured":"Pang Y, Applegate TJ (2007) Effects of dietary copper supplementation and copper source on digesta pH, calcium, zinc, and copper complex size in the gastrointestinal tract of the broiler chicken. Poult Sci 86:531\u2013537. https:\/\/doi.org\/10.1093\/ps\/86.3.531","journal-title":"Poult Sci"},{"key":"4076_CR46","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1093\/jn\/97.3.321","volume":"97","author":"BC Starcher","year":"1969","unstructured":"Starcher BC (1969) Studies on the mechanism of copper absorption in the chick. J Nutr 97:321\u2013326. https:\/\/doi.org\/10.1093\/jn\/97.3.321","journal-title":"J Nutr"},{"key":"4076_CR47","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1080\/00071668.2013.775403","volume":"54","author":"A Singh","year":"2013","unstructured":"Singh A, Walk CL, Ghosh TK, Bedford HS (2013) Effect of a novel microbial phytase on production performance and tibia mineral concentration in broiler chickens given low-calcium diets. Br Poult Sci 54:206\u2013215. https:\/\/doi.org\/10.1080\/00071668.2013.775403","journal-title":"Br Poult Sci"},{"key":"4076_CR48","doi-asserted-by":"publisher","first-page":"548","DOI":"10.3382\/ps.0400548","volume":"40","author":"CB Ammerman","year":"1961","unstructured":"Ammerman CB, Douglas CR, Davis GK, Harms RH (1961) Comparison of phosphorus availability assay techniques for chicks. Poult Sci 40:548\u2013553. https:\/\/doi.org\/10.3382\/ps.0400548","journal-title":"Poult Sci"},{"key":"4076_CR49","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1016\/j.anifeedsci.2005.04.052","volume":"123","author":"SF Li","year":"2005","unstructured":"Li SF, Luo XG, Lu L, Crenshaw TD, Bu YQ, Liu B, Kuang X, Shao GZ, Yu SX (2005) Bioavailability of organic manganese sources in broilers fed high dietary calcium. Anim Feed Sci Technol 123:703\u2013715. https:\/\/doi.org\/10.1016\/j.anifeedsci.2005.04.052","journal-title":"Anim Feed Sci Technol"},{"key":"4076_CR50","doi-asserted-by":"publisher","first-page":"6964","DOI":"10.1016\/j.psj.2020.09.053","volume":"99","author":"HTT Nguyen","year":"2020","unstructured":"Nguyen HTT, Morgan N, Roberts JR, Swick RA, Toghyani M (2020) Copper hydroxychloride is more efficacious than copper sulfate in improving broiler chicken\u2019s growth performance, both at nutritional and growth-promoting levels. Poult Sci 99:6964\u20136973. https:\/\/doi.org\/10.1016\/j.psj.2020.09.053","journal-title":"Poult Sci"},{"key":"4076_CR51","doi-asserted-by":"publisher","first-page":"3181","DOI":"10.3382\/ps\/pez045","volume":"98","author":"C Bortoluzzi","year":"2019","unstructured":"Bortoluzzi C, Vieira BS, Lumpkins B, Mathis GF, King WD, Graugnard D, Dawson KA, Applegate TJ (2019) Can dietary zinc diminish the impact of necrotic enteritis on growth performance of broiler chickens by modulating the intestinal immune-system and microbiota? Poult Sci 98:3181\u20133193. https:\/\/doi.org\/10.3382\/ps\/pez045","journal-title":"Poult Sci"},{"key":"4076_CR52","doi-asserted-by":"publisher","first-page":"102477","DOI":"10.1016\/j.psj.2023.102477","volume":"102","author":"Y Hu","year":"2023","unstructured":"Hu Y, Huang Y, Wang C, Zhang W, Qu Y, Li D, Wu W, Gao F, Zhu L, Wu B, Zhang L, Cui X, Li T, Geng Y, Liao X, Luo X (2023) The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers. Poult Sci 102:102477. https:\/\/doi.org\/10.1016\/j.psj.2023.102477","journal-title":"Poult Sci"},{"key":"4076_CR53","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1038\/s41576-020-0258-4","volume":"21","author":"C Buccitelli","year":"2020","unstructured":"Buccitelli C, Selbach M (2020) mRNAs, proteins and the emerging principles of gene expression control. Nat Rev Genet 21:630\u2013644. https:\/\/doi.org\/10.1038\/s41576-020-0258-4","journal-title":"Nat Rev Genet"},{"key":"4076_CR54","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/j.aninu.2020.09.005","volume":"7","author":"T Meng","year":"2021","unstructured":"Meng T, Gao L, Xie C, Xiang Y, Huang Y, Zhang Y, Wu X (2021) Manganese methionine hydroxy analog chelated affects growth performance, trace element deposition and expression of related transporters of broilers. Animal Nutr 7:481\u2013487. https:\/\/doi.org\/10.1016\/j.aninu.2020.09.005","journal-title":"Animal Nutr"},{"key":"4076_CR55","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1080\/00071668.2021.1887453","volume":"62","author":"TS Santos","year":"2021","unstructured":"Santos TS, Augusto KVZ, Han Y, Sartori MMP, Denadai JC, Santos CT, Sobral NC, Ro\u00e7a RO, Sartori JR (2021) High levels of copper and zinc supplementation in broiler diets on growth performance, carcass traits and apparent ileal mineral absorption. Br Poult Sci 62:579\u2013588. https:\/\/doi.org\/10.1080\/00071668.2021.1887453","journal-title":"Br Poult Sci"},{"key":"4076_CR56","doi-asserted-by":"publisher","first-page":"4961","DOI":"10.3382\/ps\/pez261","volume":"98","author":"OA Olukosi","year":"2019","unstructured":"Olukosi OA, van Kuijk SJA, Han Y (2019) Sulfate and hydroxychloride trace minerals in poultry diets\u2013comparative effects on egg production and quality in laying hens, and growth performance and oxidative stress response in broilers. Poult Sci 98:4961\u20134971. https:\/\/doi.org\/10.3382\/ps\/pez261","journal-title":"Poult Sci"}],"updated-by":[{"DOI":"10.1007\/s12011-025-04535-y","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000}}],"container-title":["Biological Trace Element Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12011-024-04076-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12011-024-04076-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12011-024-04076-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T07:28:59Z","timestamp":1738567739000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12011-024-04076-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,8]]},"references-count":56,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["4076"],"URL":"https:\/\/doi.org\/10.1007\/s12011-024-04076-w","relation":{"correction":[{"id-type":"doi","id":"10.1007\/s12011-025-04535-y","asserted-by":"object"}]},"ISSN":["0163-4984","1559-0720"],"issn-type":[{"value":"0163-4984","type":"print"},{"value":"1559-0720","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,8]]},"assertion":[{"value":"6 October 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 January 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 February 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2025","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A Correction to this paper has been published:","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"https:\/\/doi.org\/10.1007\/s12011-025-04535-y","URL":"https:\/\/doi.org\/10.1007\/s12011-025-04535-y","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Institutional Animal Care and Use Committee of the University of Georgia approved the protocol of the present trial (IACUC number: A2021-06\u2013006).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"Alessandra Monteiro is an employee of Animine (FR). The authors declare no conflict of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}