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Because Se deficiency affects large populations worldwide, Se-enriched yeasts represent a practical supplementation strategy, particularly in the organic form selenomethionine (SeMet), which is highly bioavailable. This study investigated Se accumulation and speciation in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    Thermosacc\n                    <jats:sup>\u00ae<\/jats:sup>\n                    strain cultivated in corn hydrolysate under aerobic (AE0, AE200, AE400) and anaerobic (AN0, AN200, AN400) conditions, with Na\u2082SeO\u2083 concentrations of 0, 200, and 400\u00a0mg L\u207b\u00b9. Cell performance, total Se, organic Se, and SeMet were quantified. The highest Se accumulation occurred at 400\u00a0mg L\u207b\u00b9, while 200\u00a0mg L\u207b\u00b9 favored more efficient conversion into organic forms. Aerobic metabolism supported superior intracellular concentrations (total Se: 6.15\u00a0mg g\u207b\u00b9; organic Se: 3.47\u00a0mg g\u207b\u00b9; SeMet: 2.6\u00a0mg g\u207b\u00b9), exceeding values commonly reported for commercial Se-enriched yeasts (1\u20132\u00a0mg g\u207b\u00b9). Conversion efficiency into organic Se ranged from 53% to 79%, with intermediate Na\u2082SeO\u2083 supplementation yielding the most favorable balance between accumulation and transformation. These findings show that both Na\u2082SeO\u2083 dose and metabolic pathway strongly influence Se uptake and biotransformation. Cultivation in corn hydrolysate under aerobic conditions not only promoted higher SeMet formation but also reflects conditions relevant to industrial DDGS production. This approach provides a promising strategy to valorize an abundant ethanol coproduct into functional feed with improved selenium bioavailability and reduced risk of toxicity.\n                  <\/jats:p>","DOI":"10.1007\/s12011-025-04963-w","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:18:10Z","timestamp":1767773890000},"page":"4251-4268","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biofortification of Yeast with Selenomethionine in Corn Hydrolysate: Influence of Different Metabolic Pathways"],"prefix":"10.1007","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7684-3958","authenticated-orcid":false,"given":"Layna Amorim","family":"Mota","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9929-4736","authenticated-orcid":false,"given":"Rubens Perez","family":"Calegari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2059-0568","authenticated-orcid":false,"given":"Alana Uch\u00f4a","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2606-6361","authenticated-orcid":false,"given":"Pietro","family":"Sica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Pego","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5283-2034","authenticated-orcid":false,"given":"Antonio Sampaio","family":"Baptista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3521-9136","authenticated-orcid":false,"given":"Valter","family":"Arthur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"4963_CR1","doi-asserted-by":"publisher","DOI":"10.1186\/s40795-015-0036-4","volume":"1","author":"EJM Joy","year":"2015","unstructured":"Joy EJM, Kumssa DB, Broadley MR et al (2015) Dietary mineral supplies in Malawi: spatial and socioeconomic assessment. 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