{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:44:41Z","timestamp":1784677481022,"version":"3.55.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK134583"],"award-info":[{"award-number":["DK134583"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK134583"],"award-info":[{"award-number":["DK134583"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biol Trace Elem Res"],"DOI":"10.1007\/s12011-026-05094-6","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:11:35Z","timestamp":1776258695000},"page":"6098-6105","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Heme Iron Polypeptide Resolves Iron-deficiency Anemia in Rats and Mice"],"prefix":"10.1007","volume":"204","author":[{"given":"Yue","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob S.","family":"Shine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitara","family":"Sriram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Fraga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ying","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer K.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqbal","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James F.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"key":"5094_CR1","first-page":"27","volume":"35","author":"C Uzel","year":"1998","unstructured":"Uzel C, Conrad ME (1998) Absorption of heme iron. Semin Hematol 35:27\u201334","journal-title":"Semin Hematol"},{"key":"5094_CR2","doi-asserted-by":"publisher","first-page":"G397","DOI":"10.1152\/ajpgi.00348.2013","volume":"307","author":"S Gulec","year":"2014","unstructured":"Gulec S, Anderson GJ, Collins JF (2014) Mechanistic and regulatory aspects of intestinal iron absorption. Am J Physiol Gastrointest Liver Physiol 307:G397\u2013409","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"5094_CR3","doi-asserted-by":"publisher","first-page":"G981","DOI":"10.1152\/ajpgi.00363.2005","volume":"289","author":"B Mackenzie","year":"2005","unstructured":"Mackenzie B, Garrick MD (2005) Iron imports. II. Iron uptake at the apical membrane in the intestine. Am J Physiol Gastrointest Liver Physiol 289:G981-986","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"5094_CR4","doi-asserted-by":"publisher","first-page":"4101","DOI":"10.3748\/wjg.14.4101","volume":"14","author":"AR West","year":"2008","unstructured":"West AR, Oates PS (2008) Mechanisms of heme iron absorption: current questions and controversies. World J Gastroenterol 14:4101\u20134110","journal-title":"World J Gastroenterol"},{"key":"5094_CR5","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1016\/j.cell.2005.06.025","volume":"122","author":"M Shayeghi","year":"2005","unstructured":"Shayeghi M et al (2005) Identification of an intestinal heme transporter. Cell 122:789\u2013801","journal-title":"Cell"},{"key":"5094_CR6","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/j.cell.2004.08.014","volume":"118","author":"JG Quigley","year":"2004","unstructured":"Quigley JG et al (2004) Identification of a human heme exporter that is essential for erythropoiesis. Cell 118:757\u2013766","journal-title":"Cell"},{"key":"5094_CR7","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/j.cell.2006.09.041","volume":"127","author":"A Qiu","year":"2006","unstructured":"Qiu A et al (2006) Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption. Cell 127:917\u2013928","journal-title":"Cell"},{"key":"5094_CR8","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1038\/s41586-024-07444-7","volume":"630","author":"K Ri","year":"2024","unstructured":"Ri K et al (2024) Molecular mechanism of choline and ethanolamine transport in humans. Nature 630:501\u2013508","journal-title":"Nature"},{"key":"5094_CR9","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1146\/annurev-nutr-062320-112625","volume":"42","author":"S Dutt","year":"2022","unstructured":"Dutt S, Hamza I, Bartnikas TB (2022) Molecular mechanisms of iron and heme metabolism. Annu Rev Nutr 42:311\u2013335","journal-title":"Annu Rev Nutr"},{"key":"5094_CR10","doi-asserted-by":"publisher","first-page":"G964","DOI":"10.1152\/ajpgi.00489.2004","volume":"288","author":"JF Collins","year":"2005","unstructured":"Collins JF, Franck CA, Kowdley KV, Ghishan FK (2005) Identification of differentially expressed genes in response to dietary iron deprivation in rat duodenum. Am J Physiol Gastrointest Liver Physiol 288:G964\u2013971","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"5094_CR11","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.126870","volume":"100","author":"C Fillebeen","year":"2015","unstructured":"Fillebeen C et al (2015) Mice are poor heme absorbers and do not require intestinal Hmox1 for dietary heme iron assimilation. Haematologica 100:e334-337","journal-title":"Haematologica"},{"key":"5094_CR12","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/S0021-9258(17)42488-4","volume":"261","author":"MD Maines","year":"1986","unstructured":"Maines MD, Trakshel GM, Kutty RK (1986) Characterization of two constitutive forms of rat liver microsomal heme oxygenase. Only one molecular species of the enzyme is inducible. J Biol Chem 261:411\u2013419","journal-title":"J Biol Chem"},{"key":"5094_CR13","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/S0016-5085(98)70473-1","volume":"114","author":"SM Miller","year":"1998","unstructured":"Miller SM et al (1998) Heme oxygenase 2 is present in interstitial cell networks of the mouse small intestine. Gastroenterology 114:239\u2013244","journal-title":"Gastroenterology"},{"key":"5094_CR14","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1038\/nature06934","volume":"453","author":"A Rajagopal","year":"2008","unstructured":"Rajagopal A et al (2008) Haem homeostasis is regulated by the conserved and concerted functions of HRG-1 proteins. Nature 453:1127\u20131131","journal-title":"Nature"},{"key":"5094_CR15","doi-asserted-by":"publisher","first-page":"2893","DOI":"10.1182\/blood-2014-12-567776","volume":"125","author":"T Korolnek","year":"2015","unstructured":"Korolnek T, Hamza I (2015) Macrophages and iron trafficking at the birth and death of red cells. Blood 125:2893\u20132897","journal-title":"Blood"},{"key":"5094_CR16","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.184742","author":"JK Lee","year":"2025","unstructured":"Lee JK et al (2025) Development of rat and mouse models of heme-iron absorption. JCI Insight. https:\/\/doi.org\/10.1172\/jci.insight.184742","journal-title":"JCI Insight"},{"key":"5094_CR17","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1111\/jcpt.12281","volume":"40","author":"RB Dull","year":"2015","unstructured":"Dull RB, Davis E (2015) Heme iron polypeptide for the management of anaemia of chronic kidney disease. J Clin Pharm Ther 40:386\u2013390","journal-title":"J Clin Pharm Ther"},{"key":"5094_CR18","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1016\/S0271-5317(00)00215-3","volume":"20","author":"Anonymous","year":"2000","unstructured":"Anonymous (2000) Clinical studies of HIP: an oral heme-iron product. Nutr Res 20:1279\u20131286","journal-title":"Nutr Res"},{"key":"5094_CR19","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/j.1365-2141.1957.tb05785.x","volume":"3","author":"ST CALLENDER","year":"1957","unstructured":"CALLENDER ST, MALLETT BJ, SMITH MD (1957) Absorption of haemoglobin iron. Br J Haematol 3:186\u2013192","journal-title":"Br J Haematol"},{"key":"5094_CR20","doi-asserted-by":"publisher","first-page":"2214","DOI":"10.1093\/jn\/133.7.2214","volume":"133","author":"F Pizarro","year":"2003","unstructured":"Pizarro F, Olivares M, Hertrampf E, Mazariegos DI, Arredondo M (2003) Heme-iron absorption is saturable by heme-iron dose in women. J Nutr 133:2214\u20132217","journal-title":"J Nutr"},{"key":"5094_CR21","first-page":"471","volume":"47","author":"JC Wyllie","year":"1982","unstructured":"Wyllie JC, Kaufman N (1982) An electron microscopic study of heme uptake by rat duodenum. Lab Invest 47:471\u2013476","journal-title":"Lab Invest"},{"key":"5094_CR22","first-page":"318","volume":"47","author":"RE B\u00f6ni","year":"1993","unstructured":"B\u00f6ni RE, Huch B\u00f6ni RA, Galbraith RA, Drummond GS, Kappas A (1993) Tin-mesoporphyrin inhibits heme oxygenase activity and heme-iron absorption in the intestine. Pharmacology 47:318\u2013329","journal-title":"Pharmacology"},{"key":"5094_CR23","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/0014-4800(81)90070-8","volume":"34","author":"RT Parmley","year":"1981","unstructured":"Parmley RT, Barton JC, Conrad ME, Austin RL, Holland RM (1981) Ultrastructural cytochemistry and radioautography of hemoglobin\u2013iron absorption. Exp Mol Pathol 34:131\u2013144","journal-title":"Exp Mol Pathol"},{"key":"5094_CR24","doi-asserted-by":"publisher","first-page":"58","DOI":"10.7150\/ijbs.4.58","volume":"4","author":"AG Quintero-Guti\u00e9rrez","year":"2008","unstructured":"Quintero-Guti\u00e9rrez AG, Gonz\u00e1lez-Rosendo G, S\u00e1nchez-Mu\u00f1oz J, Polo-Pozo J, Rodr\u00edguez-Jerez JJ (2008) Bioavailability of heme iron in biscuit filling using piglets as an animal model for humans. Int J Biol Sci 4:58\u201362","journal-title":"Int J Biol Sci"},{"key":"5094_CR25","first-page":"944","volume":"65","author":"RM Bannerman","year":"1965","unstructured":"Bannerman RM (1965) QUANTITATIVE ASPECTS OF HEMOGLOBIN-IRON ABSORPTION. J Lab Clin Med 65:944\u2013950","journal-title":"J Lab Clin Med"},{"key":"5094_CR26","doi-asserted-by":"publisher","first-page":"4478","DOI":"10.1021\/jf021246l","volume":"51","author":"J Matsumoto","year":"2003","unstructured":"Matsumoto J, Mori N, Doi M, Kishida T, Ebihara K (2003) Evaluation of iron bioavailability from bonito dark muscle using anemic rats. J Agric Food Chem 51:4478\u20134482","journal-title":"J Agric Food Chem"},{"key":"5094_CR27","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1016\/S0016-5085(70)80123-8","volume":"58","author":"MS Wheby","year":"1970","unstructured":"Wheby MS, Suttle GE, Ford KT (1970) Intestinal absorption of hemoglobin iron. Gastroenterology 58:647\u2013654","journal-title":"Gastroenterology"},{"key":"5094_CR28","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1039\/C3FO60292C","volume":"5","author":"N Tang","year":"2014","unstructured":"Tang N, Chen LQ, Zhuang H (2014) Effects of heme iron enriched peptide on iron deficiency anemia in rats. Food Funct 5:390\u2013399","journal-title":"Food Funct"},{"key":"5094_CR29","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/j.jnutbio.2005.01.003","volume":"16","author":"N Vaghefi","year":"2005","unstructured":"Vaghefi N et al (2005) Iron absorption from concentrated hemoglobin hydrolysate by rat. J Nutr Biochem 16:347\u2013352","journal-title":"J Nutr Biochem"},{"key":"5094_CR30","doi-asserted-by":"publisher","first-page":"G535","DOI":"10.1152\/ajpgi.00169.2017","volume":"313","author":"JH Ha","year":"2017","unstructured":"Ha JH, Doguer C, Collins JF (2017) Consumption of a High-Iron Diet Disrupts Homeostatic Regulation of Intestinal Copper Absorption in Adolescent Mice. Am J Physiol Gastrointest Liver Physiol 313:G535\u2013G360","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"5094_CR31","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1093\/jn\/130.3.621","volume":"130","author":"MD Knutson","year":"2000","unstructured":"Knutson MD, Walter PB, Ames BN, Viteri FE (2000) Both iron deficiency and daily iron supplements increase lipid peroxidation in rats. J Nutr 130:621\u2013628","journal-title":"J Nutr"},{"key":"5094_CR32","first-page":"9","volume":"33","author":"JD Torrance","year":"1968","unstructured":"Torrance JD, Bothwell TH (1968) A simple technique for measuring storage iron concentrations in formalinised liver samples. S Afr J Med Sci 33:9\u201311","journal-title":"S Afr J Med Sci"},{"key":"5094_CR33","doi-asserted-by":"publisher","first-page":"9429","DOI":"10.1021\/acs.analchem.0c00415","volume":"92","author":"MT Hopp","year":"2020","unstructured":"Hopp MT et al (2020) Heme Determination and Quantification Methods and Their Suitability for Practical Applications and Everyday Use. Anal Chem 92:9429\u20139440","journal-title":"Anal Chem"},{"key":"5094_CR34","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3945\/jn.113.183160","volume":"144","author":"S Gulec","year":"2014","unstructured":"Gulec S, Collins JF (2014) Silencing the Menkes copper-transporting ATPase (Atp7a) gene in rat intestinal epithelial (IEC-6) cells increases iron flux via transcriptional induction of ferroportin 1 (Fpn1). J Nutr 144:12\u201319","journal-title":"J Nutr"}],"container-title":["Biological Trace Element Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12011-026-05094-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12011-026-05094-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12011-026-05094-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T20:03:05Z","timestamp":1784059385000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12011-026-05094-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,15]]},"references-count":34,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["5094"],"URL":"https:\/\/doi.org\/10.1007\/s12011-026-05094-6","relation":{},"ISSN":["1559-0720"],"issn-type":[{"value":"1559-0720","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,15]]},"assertion":[{"value":"30 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing Interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}