{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:46:41Z","timestamp":1789152401192,"version":"build-2803163510"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T00:00:00Z","timestamp":1481155200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T00:00:00Z","timestamp":1481155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100000861","name":"Sparks","doi-asserted-by":"publisher","award":["14SAP01"],"award-info":[{"award-number":["14SAP01"]}],"id":[{"id":"10.13039\/501100000861","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Biochem Biophys"],"published-print":{"date-parts":[[2017,3]]},"DOI":"10.1007\/s12013-016-0775-0","type":"journal-article","created":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T03:52:06Z","timestamp":1481169126000},"page":"95-102","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Nano-Mechanical Characterization of Ataxia Telangiectasia Cells Treated with Dexamethasone"],"prefix":"10.1007","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5206-6296","authenticated-orcid":false,"given":"Michele","family":"Menotta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Biagiotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Bartolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianchi","family":"Marzia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Orazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Germani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Chessa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Magnani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,8]]},"reference":[{"issue":"8-9","key":"775_CR1","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1016\/j.dnarep.2004.04.002","volume":"3","author":"RT Abraham","year":"2004","unstructured":"Abraham, R. T. (2004). PI 3-kinase related kinases: \u2018Big\u2019 players in stress-induced signaling pathways. DNA Repair, 3(8-9), 883\u2013887.","journal-title":"DNA Repair"},{"issue":"4","key":"775_CR2","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/S1071-9091(98)80007-7","volume":"5","author":"TO Crawford","year":"1998","unstructured":"Crawford, T. O. (1998). Ataxia telangiectasia. Seminars in Pediatric Neurology, 5(4), 287\u2013294.","journal-title":"Seminars in Pediatric Neurology"},{"issue":"4","key":"775_CR3","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1385\/NMM:8:4:495","volume":"8","author":"PO Frappart","year":"2006","unstructured":"Frappart, P. O., & McKinnon, P. J. (2006). Ataxia-telangiectasia and related diseases. Neuromolecular Medicine, 8(4), 495\u2013511.","journal-title":"Neuromolecular Medicine"},{"issue":"3","key":"775_CR4","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1111\/j.1468-1331.2008.02060.x","volume":"15","author":"T Broccoletti","year":"2008","unstructured":"Broccoletti, T., Del, G. E., & Amorosi, S., et al. (2008). Steroid-induced improvement of neurological signs in ataxia-telangiectasia patients. European Journal of Neurology: The Official Journal of the European Federation of Neurological Societies, 15(3), 223\u2013228.","journal-title":"European Journal of Neurology: The Official Journal of the European Federation of Neurological Societies"},{"issue":"4","key":"775_CR5","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1111\/j.1468-1331.2010.03203.x","volume":"18","author":"T Broccoletti","year":"2011","unstructured":"Broccoletti, T., Del, G. E., & Cirillo, E., et al. (2011). Efficacy of very-low-dose betamethasone on neurological symptoms in ataxia-telangiectasia. European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies, 18(4), 564\u2013570.","journal-title":"European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies"},{"issue":"10","key":"775_CR6","doi-asserted-by":"publisher","first-page":"1479","DOI":"10.1001\/archneur.63.10.1479","volume":"63","author":"S Buoni","year":"2006","unstructured":"Buoni, S., Zannolli, R., & Sorrentino, L., et al. (2006). Betamethasone and improvement of neurological symptoms in ataxia-telangiectasia. Archives of Neurology, 63(10), 1479\u20131482.","journal-title":"Archives of Neurology"},{"issue":"6","key":"775_CR7","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1111\/j.1468-1331.2009.02600.x","volume":"16","author":"I Russo","year":"2009","unstructured":"Russo, I., Cosentino, C., & Del, G. E., et al. (2009). In ataxia-teleangiectasia betamethasone response is inversely correlated to cerebellar atrophy and directly to antioxidative capacity. European Journal of Neurology: The Official Journal of the European Federation of Neurological Societies, 16(6), 755\u2013759.","journal-title":"European Journal of Neurology: The Official Journal of the European Federation of Neurological Societies"},{"issue":"10","key":"775_CR8","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1002\/mds.25126","volume":"27","author":"R Zannolli","year":"2012","unstructured":"Zannolli, R., Buoni, S., & Betti, G., et al. (2012). A randomized trial of oral betamethasone to reduce ataxia symptoms in ataxia telangiectasia. Movement Disorders: Official Journal of the Movement Disorder Society, 27(10), 1312\u20131316.","journal-title":"Movement Disorders: Official Journal of the Movement Disorder Society"},{"key":"775_CR9","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/1750-1172-9-5","volume":"9","author":"L Chessa","year":"2014","unstructured":"Chessa, L., Leuzzi, V., & Plebani, A., et al. (2014). Intra-erythrocyte infusion of dexamethasone reduces neurological symptoms in ataxia teleangiectasia patients: Results of a phase 2 trial. Orphanet Journal of Rare Diseases, 9, 5.","journal-title":"Orphanet Journal of Rare Diseases"},{"issue":"3","key":"775_CR10","first-page":"e98","volume":"2","author":"V Leuzzi","year":"2015","unstructured":"Leuzzi, V., Micheli, R., & D\u2019Agnano, D., et al. (2015). Positive effect of erythrocyte-delivered dexamethasone in ataxia-telangiectasia. Neurology:. Neuroimmunoogy & Neuroinflammation, 2(3), e98.","journal-title":"Neurology:. Neuroimmunoogy & Neuroinflammation"},{"issue":"1-2","key":"775_CR11","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s11010-014-2013-7","volume":"392","author":"S Biagiotti","year":"2014","unstructured":"Biagiotti, S., Menotta, M., & Giacomini, E., et al. (2014). Forward subtractive libraries containing genes transactivated by dexamethasone in ataxia-telangiectasia lymphoblastoid cells. Molecular and Cellular Biochemistry, 392(1-2), 13\u201330.","journal-title":"Molecular and Cellular Biochemistry"},{"issue":"49","key":"775_CR12","doi-asserted-by":"publisher","first-page":"41352","DOI":"10.1074\/jbc.M112.344473","volume":"287","author":"M Menotta","year":"2012","unstructured":"Menotta, M., Biagiotti, S., & Bianchi, M., et al. (2012). Dexamethasone partially rescues ataxia telangiectasia-mutated (ATM) deficiency in ataxia telangiectasia by promoting a shortened protein variant retaining kinase activity. The Journal of Biological Chemistry, 287(49), 41352\u201341363.","journal-title":"The Journal of Biological Chemistry"},{"key":"775_CR13","unstructured":"Dufrene, Y. F., Boland, T., Schneider, J. W., et al. (1998). Characterization of the physical properties of model biomembranes at the nanometer scale with the atomic force microscope. Faraday Discussions (111), 79\u201394."},{"issue":"6","key":"775_CR14","doi-asserted-by":"publisher","first-page":"3289","DOI":"10.1016\/S0006-3495(99)77481-3","volume":"76","author":"E Nagao","year":"1999","unstructured":"Nagao, E., & Dvorak, J. A. (1999). Phase imaging by atomic force microscopy: Analysis of living homoiothermic vertebrate cells. Biophysical Journal, 76(6), 3289\u20133297.","journal-title":"Biophysical Journal"},{"issue":"3","key":"775_CR15","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1016\/S0006-3495(93)81433-4","volume":"64","author":"M Radmacher","year":"1993","unstructured":"Radmacher, M., Tillmann, R. W., & Gaub, H. E. (1993). Imaging viscoelasticity by force modulation with the atomic force microscope. Biophysical Journal, 64(3), 735\u2013742.","journal-title":"Biophysical Journal"},{"issue":"1","key":"775_CR16","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1006\/bbrc.2000.2742","volume":"272","author":"K Mitsui","year":"2000","unstructured":"Mitsui, K., Nakajima, K., & Arakawa, H., et al. (2000). Dynamic measurement of single protein\u2019s mechanical properties. Biochemical and Biophysical Research Communications, 272(1), 55\u201363.","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"3","key":"775_CR17","first-page":"255","volume":"50","author":"A Simon","year":"2004","unstructured":"Simon, A., Cohen-Bouhacina, T., & Aime, J. P., et al. (2004). Heterogeneous cell mechanical properties: An atomic force microscopy study. Cellular and Molecular Biology (Noisy. -le-grand), 50(3), 255\u2013266.","journal-title":"Cellular and Molecular Biology (Noisy. -le-grand)"},{"issue":"12","key":"775_CR18","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1007\/BF02621085","volume":"22","author":"F Pelloquin","year":"1986","unstructured":"Pelloquin, F., Lamelin, J. P., & Lenoir, G. M. (1986). Human B lymphocytes immortalization by Epstein-Barr virus in the presence of cyclosporin A. In Vitro Cellular & Developmental Biology: Journal of the Tissue Culture Association, 22(12), 689\u2013694.","journal-title":"In Vitro Cellular & Developmental Biology: Journal of the Tissue Culture Association"},{"issue":"10","key":"775_CR19","first-page":"2544","volume":"54","author":"YK Huo","year":"1994","unstructured":"Huo, Y. K., Wang, Z., & Hong, J. H., et al. (1994). Radiosensitivity of ataxia-telangiectasia, X-linked agammaglobulinemia, and related syndromes using a modified colony survival assay. Cancer Research, 54(10), 2544\u20132547.","journal-title":"Cancer Research"},{"issue":"7","key":"775_CR20","doi-asserted-by":"publisher","first-page":"1868","DOI":"10.1063\/1.1143970","volume":"64","author":"JL Hutter","year":"1993","unstructured":"Hutter, J. L., & Bechhoefer, J. (1993). Calibration of atomic-force microscope tips. Review of Scientific Instruments, 64(7), 1868\u20131873.","journal-title":"Review of Scientific Instruments"},{"issue":"7","key":"775_CR21","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), 671\u2013675.","journal-title":"Nature Methods"},{"issue":"3","key":"775_CR22","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1115\/1.2891183","volume":"112","author":"M Sato","year":"1990","unstructured":"Sato, M., Theret, D. P., & Wheeler, L. T., et al. (1990). Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties. Journal of Biomechanical Engineering, 112(3), 263\u2013268.","journal-title":"Journal of Biomechanical Engineering"},{"issue":"7","key":"775_CR23","doi-asserted-by":"publisher","first-page":"1502","DOI":"10.1016\/j.bpj.2014.07.073","volume":"107","author":"N Nijenhuis","year":"2014","unstructured":"Nijenhuis, N., Zhao, X., & Carisey, A., et al. (2014). Combining AFM and acoustic probes to reveal changes in the elastic stiffness tensor of living cells. Biophysical Journal, 107(7), 1502\u20131512.","journal-title":"Biophysical Journal"},{"issue":"8","key":"775_CR24","doi-asserted-by":"publisher","first-page":"1987","DOI":"10.1073\/pnas.1508920113","volume":"113","author":"ME Fay","year":"2016","unstructured":"Fay, M. E., Myers, D. R., & Kumar, A., et al. (2016). Cellular softening mediates leukocyte demargination and trafficking, thereby increasing clinical blood counts. Proceedings of the National Academy of Sciences of the United States of America, 113(8), 1987\u20131992.","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"8","key":"775_CR25","doi-asserted-by":"publisher","first-page":"4447","DOI":"10.1167\/iovs.15-16739","volume":"56","author":"VK Raghunathan","year":"2015","unstructured":"Raghunathan, V. K., Morgan, J. T., & Park, S. A., et al. (2015). Dexamethasone stiffens trabecular meshwork, trabecular meshwork cells, and matrix. Investigative Ophthalmology & Visual Science, 56(8), 4447\u20134459.","journal-title":"Investigative Ophthalmology & Visual Science"},{"issue":"7","key":"775_CR26","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1042\/CS20140758","volume":"129","author":"MA Silva","year":"2015","unstructured":"Silva, M. A., Cau, S. B., & Lopes, R. A., et al. (2015). Mineralocorticoid receptor blockade prevents vascular remodelling in a rodent model of type 2 diabetes mellitus. Clinical Science (London, England: 1979), 129(7), 533\u2013545.","journal-title":"Clinical Science (London, England: 1979)"},{"issue":"Pt 9","key":"775_CR27","doi-asserted-by":"publisher","first-page":"1926","DOI":"10.1242\/jcs.02886","volume":"119","author":"H Oberleithner","year":"2006","unstructured":"Oberleithner, H., Riethmuller, C., & Ludwig, T., et al. (2006). Differential action of steroid hormones on human endothelium. Journal of Cell Science, 119(Pt 9), 1926\u20131932.","journal-title":"Journal of Cell Science"},{"issue":"2","key":"775_CR28","doi-asserted-by":"publisher","first-page":"904","DOI":"10.1016\/S0006-3495(00)76346-6","volume":"79","author":"S Krol","year":"2000","unstructured":"Krol, S., Ross, M., & Sieber, M., et al. (2000). Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B. Biophysical Journal, 79(2), 904\u2013918.","journal-title":"Biophysical Journal"},{"issue":"1","key":"775_CR29","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1679\/aohc.61.57","volume":"61","author":"S Sasaki","year":"1998","unstructured":"Sasaki, S., Morimoto, M., & Haga, H., et al. (1998). Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy. Archives of Histology and Cytology, 61(1), 57\u201363.","journal-title":"Archives of Histology and Cytology"},{"issue":"6","key":"775_CR30","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1021\/bm034259w","volume":"4","author":"N Fang","year":"2003","unstructured":"Fang, N., & Chan, V. (2003). Chitosan-induced restructuration of a mica-supported phospholipid bilayer: An atomic force microscopy study. Biomacromolecules, 4(6), 1596\u20131604.","journal-title":"Biomacromolecules"},{"issue":"10","key":"775_CR31","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1002\/jemt.20486","volume":"70","author":"M Girasole","year":"2007","unstructured":"Girasole, M., Cricenti, A., & Generosi, R., et al. (2007). Different membrane modifications revealed by atomic force\/lateral force microscopy after doping of human pancreatic cells with Cd, Zn, or Pb. Microscopy Research and Technique, 70(10), 912\u2013917.","journal-title":"Microscopy Research and Technique"},{"key":"775_CR32","first-page":"2603","volume":"4","author":"Y Shen","year":"2004","unstructured":"Shen, Y., Sun, J. L., & Zhang, A., et al. (2004). Shape recovering of live endothelial cell under atomic force microscopy imaging. Conference Proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, 4, 2603\u20132606.","journal-title":"Conference Proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference"},{"key":"775_CR33","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.jmbbm.2015.11.039","volume":"59","author":"B Fallqvist","year":"2016","unstructured":"Fallqvist, B., Fielden, M. L., & Pettersson, T., et al. (2016). Experimental and computational assessment of F-actin influence in regulating cellular stiffness and relaxation behaviour of fibroblasts. Journal of the Mechanical Behavior of Biomedical Materials, 59, 168\u2013184.","journal-title":"Journal of the Mechanical Behavior of Biomedical Materials"},{"key":"775_CR34","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.actbio.2015.08.028","volume":"27","author":"M Tartibi","year":"2015","unstructured":"Tartibi, M., Liu, Y. X., & Liu, G. Y., et al. (2015). Single-cell mechanics--An experimental-computational method for quantifying the membrane-cytoskeleton elasticity of cells. Acta Biomaterialia, 27, 224\u2013235.","journal-title":"Acta Biomaterialia"},{"key":"775_CR35","first-page":"831","volume":"2010","author":"AS Ribeiro","year":"2010","unstructured":"Ribeiro, A. S., & Dahl, K. N. (2010). The nucleus as a central structure in defining the mechanical properties of stem cells. Conference Proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, 2010, 831\u2013834.","journal-title":"Conference Proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference"},{"issue":"2","key":"775_CR36","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/S0021-9290(01)00201-9","volume":"35","author":"N Caille","year":"2002","unstructured":"Caille, N., Thoumine, O., & Tardy, Y., et al. (2002). Contribution of the nucleus to the mechanical properties of endothelial cells. Journal of Biomechanics, 35(2), 177\u2013187.","journal-title":"Journal of Biomechanics"},{"issue":"3","key":"775_CR37","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1006\/bbrc.2000.2360","volume":"269","author":"F Guilak","year":"2000","unstructured":"Guilak, F., Tedrow, J. R., & Burgkart, R. (2000). Viscoelastic properties of the cell nucleus. Biochemical and Biophysical Research Communications, 269(3), 781\u2013786.","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"4","key":"775_CR38","doi-asserted-by":"publisher","first-page":"3821","DOI":"10.1021\/nn500553z","volume":"8","author":"H Liu","year":"2014","unstructured":"Liu, H., Wen, J., & Xiao, Y., et al. (2014). In situ mechanical characterization of the cell nucleus by atomic force microscopy. ACS Nano, 8(4), 3821\u20133828.","journal-title":"ACS Nano"},{"issue":"4 Pt 1","key":"775_CR39","doi-asserted-by":"crossref","first-page":"C1190","DOI":"10.1152\/ajpcell.1996.270.4.C1190","volume":"270","author":"RS Benson","year":"1996","unstructured":"Benson, R. S., Heer, S., & Dive, C., et al. (1996). Characterization of cell volume loss in CEM-C7A cells during dexamethasone-induced apoptosis. The American Journal of Physiology, 270(4 Pt 1), C1190\u2013C1203.","journal-title":"The American Journal of Physiology"}],"container-title":["Cell Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12013-016-0775-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12013-016-0775-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12013-016-0775-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T18:23:55Z","timestamp":1602267835000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12013-016-0775-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,8]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["775"],"URL":"https:\/\/doi.org\/10.1007\/s12013-016-0775-0","relation":{},"ISSN":["1085-9195","1559-0283"],"issn-type":[{"value":"1085-9195","type":"print"},{"value":"1559-0283","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,8]]},"assertion":[{"value":"12 September 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 November 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 December 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}