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Our study examines the impact of <jats:italic>ERG6<\/jats:italic> gene deletion on the membrane composition and physicochemical properties of the pathogenic yeast <jats:italic>Candida glabrata<\/jats:italic>. Specifically, we investigated changes in selected sterol content, phospholipid composition, transmembrane potential, and <jats:italic>PDR16<\/jats:italic> gene activity. Sterol levels were measured using high-performance liquid chromatography, the phospholipid profile was analysed via thin-layer chromatography, transmembrane potential was assessed with fluorescence spectroscopy, and gene expression levels were determined by quantitative PCR. Our findings revealed a depletion of ergosterol, increased zymosterol and eburicol content, an increased phosphatidylcholine and a reduced phosphatidylethanolamine content in the <jats:italic>\u0394erg6<\/jats:italic> strain compared to the <jats:italic>wt<\/jats:italic>. Additionally, the <jats:italic>\u0394erg6<\/jats:italic> strain exhibited membrane hyperpolarization without changes in <jats:italic>PDR16<\/jats:italic> expression. Furthermore, the <jats:italic>\u0394erg6<\/jats:italic> strain showed increased sensitivity to the antifungals myriocin and aureobasidine A. These results suggest that <jats:italic>ERG6<\/jats:italic> gene deletion leads to significant alterations in membrane composition and may activates an alternative ergosterol synthesis pathway in the <jats:italic>C. glabrata \u0394erg6<\/jats:italic> deletion mutant.<\/jats:p>","DOI":"10.1007\/s12013-024-01599-w","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T03:01:58Z","timestamp":1730343718000},"page":"1909-1918","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Impact of ERG6 Gene Deletion on Membrane Composition and Properties in the Pathogenic Yeast Candida glabrata"],"prefix":"10.1007","volume":"83","author":[{"given":"J.","family":"Jacko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"M.","family":"Morvov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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