{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:10:42Z","timestamp":1792624242661,"version":"4.5.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2018,5,4]],"date-time":"2018-05-04T00:00:00Z","timestamp":1525392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Rev and Rep"],"published-print":{"date-parts":[[2018,10]]},"DOI":"10.1007\/s12015-018-9820-2","type":"journal-article","created":{"date-parts":[[2018,5,4]],"date-time":"2018-05-04T18:26:48Z","timestamp":1525458408000},"page":"734-743","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Development of a Novel Large Animal Model to Evaluate Human Dental Pulp Stem Cells for Articular Cartilage Treatment"],"prefix":"10.1007","volume":"14","author":[{"given":"Tiago Lazzaretti","family":"Fernandes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Shimomura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Asperti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Cristina Gomes","family":"Pinheiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helo\u00edsa Vasconcellos Amaral","family":"Caetano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Regina G. C. M.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norimasa","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaldo Jos\u00e9","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Franco","family":"Bueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,5,4]]},"reference":[{"issue":"6","key":"9820_CR1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1308\/003588412X13171221592573","volume":"94","author":"JR Perera","year":"2012","unstructured":"Perera, J. R., Gikas, P. D., & Bentley, G. (2012). The present state of treatments for articular cartilage defects in the knee. Annals of the Royal College of Surgeons of England [Internet], 94(6), 381\u2013387. [cited 2015 Nov 24]; Available from: \n                    http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=3954317&tool=pmcentrez&rendertype=abstract\n                    \n                  .","journal-title":"Annals of the Royal College of Surgeons of England [Internet]"},{"issue":"4","key":"9820_CR2","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/S0749-8063(97)90124-9","volume":"13","author":"WW Curl","year":"1997","unstructured":"Curl, W. W., Krome, J., Gordon, E. S., Rushing, J., Smith, B. P., & Poehling, G. G. (1997). Cartilage injuries: A review of 31,516 knee arthroscopies. Arthroscopy, 13(4), 456\u2013460.","journal-title":"Arthroscopy"},{"issue":"10","key":"9820_CR3","doi-asserted-by":"publisher","first-page":"1795","DOI":"10.1249\/MSS.0b013e3181d9eea0","volume":"42","author":"DC Flanigan","year":"2010","unstructured":"Flanigan, D. C., Harris, J. D., Trinh, T. Q., Siston, R. A., & Brophy, R. H. (2010). Prevalence of chondral defects in athletes\u2019 knees: A systematic review. Medicine and Science in Sports and Exercise, 42(10), 1795\u20131801.","journal-title":"Medicine and Science in Sports and Exercise"},{"issue":"4","key":"9820_CR4","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1007\/s00167-010-1072-x","volume":"18","author":"AH Gomoll","year":"2010","unstructured":"Gomoll, A. H., Madry, H., Knutsen, G., et al. (2010). The subchondral bone in articular cartilage repair: current problems in the surgical management. Knee Surgery, Sports Traumatology, Arthroscopy [Internet], 18(4), 434\u2013447. [cited 2015 Nov 24]; Available from: \n                    http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=2839476&tool=pmcentrez&rendertype=abstract\n                    \n                  .","journal-title":"Knee Surgery, Sports Traumatology, Arthroscopy [Internet]"},{"issue":"7","key":"9820_CR5","doi-asserted-by":"publisher","first-page":"467","DOI":"10.5435\/JAAOS-22-07-467","volume":"22","author":"J Shen","year":"2014","unstructured":"Shen, J., & Chen, D. (2014). Recent progress in osteoarthritis research. The Journal of the American Academy of Orthopaedic Surgeons, 22(7), 467\u2013468.","journal-title":"The Journal of the American Academy of Orthopaedic Surgeons"},{"key":"9820_CR6","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/827149","volume":"2012","author":"SH Zainal Ariffin","year":"2012","unstructured":"Zainal Ariffin, S. H., Kermani, S., Megat Abdul Wahab, R., Senafi, S., Zainal Ariffin, Z., & Abdul Razak, M. (2012). In vitro chondrogenesis transformation study of mouse dental pulp stem cells. ScientificWorldJournal, 2012, 827149.","journal-title":"ScientificWorldJournal"},{"issue":"5","key":"9820_CR7","doi-asserted-by":"publisher","first-page":"303","DOI":"10.5435\/JAAOS-21-05-303","volume":"21","author":"RS Tuan","year":"2013","unstructured":"Tuan, R. S., Chen, A. F., & Klatt, B. A. (2013). Cartilage regeneration. The Journal of the American Academy of Orthopaedic Surgeons, 21(5), 303\u2013311.","journal-title":"The Journal of the American Academy of Orthopaedic Surgeons"},{"issue":"2 Suppl","key":"9820_CR8","doi-asserted-by":"publisher","first-page":"13S","DOI":"10.1177\/1947603515571002","volume":"6","author":"K Shimomura","year":"2015","unstructured":"Shimomura, K., Ando, W., Moriguchi, Y., Sugita, N., Yasui, Y., Koizumi, K., Fujie, H., Hart, D. A., Yoshikawa, H., & Nakamura, N. (2015). Next generation mesenchymal stem cell (MSC)-based cartilage repair using scaffold-free tissue engineered constructs generated with synovial mesenchymal stem cells. Cartilage, 6(2 Suppl), 13S\u201329S.","journal-title":"Cartilage"},{"issue":"12","key":"9820_CR9","doi-asserted-by":"publisher","first-page":"2041","DOI":"10.1089\/ten.tea.2008.0015","volume":"14","author":"W Ando","year":"2008","unstructured":"Ando, W., Tateishi, K., Katakai, D., et al. (2008). In vitro generation of a scaffold-free tissue-engineered construct (TEC) derived from human synovial mesenchymal stem cells: biological and mechanical properties and further chondrogenic potential. Tissue Engineering Part A [Internet], 14(12), 2041\u20132049. [cited 2016 Oct 25]; Available from: \n                    http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18636944\n                    \n                  .","journal-title":"Tissue Engineering Part A [Internet]"},{"issue":"1","key":"9820_CR10","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1097\/scs.0b013e31815c8a54","volume":"19","author":"A Mendon\u00e7a Costa de","year":"2008","unstructured":"de Mendon\u00e7a Costa, A., Bueno, D. F., Martins, M. T., Kerkis, I., Kerkis, A., Fanganiello, R. D., Cerruti, H., Alonso, N., & Passos-Bueno, M. R. (2008). Reconstruction of large cranial defects in nonimmunosuppressed experimental design with human dental pulp stem cells. The Journal of Craniofacial Surgery, 19(1), 204\u2013210.","journal-title":"The Journal of Craniofacial Surgery"},{"issue":"2","key":"9820_CR11","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1089\/ten.teb.2011.0327","volume":"18","author":"I Kerkis","year":"2012","unstructured":"Kerkis, I., & Caplan, A. I. (2012). Stem cells in dental pulp of deciduous teeth. Tissue Engineering. Part B, Reviews, 18(2), 129\u2013138.","journal-title":"Tissue Engineering. Part B, Reviews"},{"issue":"1","key":"9820_CR12","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s00441-013-1630-x","volume":"353","author":"P Hilkens","year":"2013","unstructured":"Hilkens, P., Gervois, P., Fanton, Y., Vanormelingen, J., Martens, W., Struys, T., Politis, C., Lambrichts, I., & Bronckaers, A. (2013). Effect of isolation methodology on stem cell properties and multilineage differentiation potential of human dental pulp stem cells. Cell and Tissue Research, 353(1), 65\u201378.","journal-title":"Cell and Tissue Research"},{"issue":"6","key":"9820_CR13","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1097\/01.tp.0000173794.72151.88","volume":"80","author":"L Pierdomenico","year":"2005","unstructured":"Pierdomenico, L., Bonsi, L., Calvitti, M., Rondelli, D., Arpinati, M., Chirumbolo, G., Becchetti, E., Marchionni, C., Alviano, F., Fossati, V., Staffolani, N., Franchina, M., Grossi, A., & Bagnara, G. P. (2005). Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation, 80(6), 836\u2013842.","journal-title":"Transplantation"},{"issue":"10","key":"9820_CR14","doi-asserted-by":"publisher","first-page":"2813","DOI":"10.1089\/ten.2006.12.2813","volume":"12","author":"W Zhang","year":"2006","unstructured":"Zhang, W., Walboomers, X. F., Shi, S., Fan, M., & Jansen, J. A. (2006). Multilineage differentiation potential of stem cells derived from human dental pulp after cryopreservation. Tissue Engineering, 12(10), 2813\u20132823.","journal-title":"Tissue Engineering"},{"issue":"31","key":"9820_CR15","doi-asserted-by":"publisher","first-page":"8004","DOI":"10.1016\/j.biomaterials.2010.07.017","volume":"31","author":"K Shimomura","year":"2010","unstructured":"Shimomura, K., Ando, W., Tateishi, K., et al. (2010). The influence of skeletal maturity on allogenic synovial mesenchymal stem cell-based repair of cartilage in a large animal model. Biomaterials [Internet], 31(31), 8004\u20138011. Available from: \n                    https:\/\/doi.org\/10.1016\/j.biomaterials.2010.07.017\n                    \n                  .","journal-title":"Biomaterials [Internet]"},{"issue":"10","key":"9820_CR16","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1016\/j.exphem.2010.06.008","volume":"38","author":"D Agay","year":"2010","unstructured":"Agay, D., Scherthan, H., Forcheron, F., Grenier, N., H\u00e9rodin, F., Meineke, V., & Drouet, M. (2010). Multipotent mesenchymal stem cell grafting to treat cutaneous radiation syndrome: Development of a new minipig model. Experimental Hematology, 38(10), 945\u2013956.","journal-title":"Experimental Hematology"},{"issue":"9","key":"9820_CR17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025210","volume":"6","author":"M Moroni","year":"2011","unstructured":"Moroni, M., Lombardini, E., Salber, R., Kazemzedeh, M., Nagy, V., Olsen, C., & Whitnall, M. H. (2011). Hematological changes as prognostic indicators of survival: Similarities between Gottingen minipigs, humans, and other large animal models. PLoS One, 6(9), e25210.","journal-title":"PLoS One"},{"issue":"3","key":"9820_CR18","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1590\/1678-775720130526","volume":"22","author":"RT Stramandinoli-Zanicotti","year":"2014","unstructured":"Stramandinoli-Zanicotti, R. T., Carvalho, A. L., Rebelatto, C. L. K., et al. (2014). Brazilian minipig as a large-animal model for basic research and stem cell-based tissue engineering. Characterization and in vitro differentiation of bone marrow-derived mesenchymal stem cells. Journal of Applied Oral Science, 22(3), 218\u2013227.","journal-title":"Journal of Applied Oral Science"},{"issue":"2","key":"9820_CR19","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1089\/ten.tea.2007.0417","volume":"15","author":"DF Bueno","year":"2009","unstructured":"Bueno, D. F., Kerkis, I., Costa, A. M., Martins, M. T., Kobayashi, G. S., Zucconi, E., Fanganiello, R. D., Salles, F. T., Almeida, A. B., do Amaral, C. E. R., Alonso, N., & Passos-Bueno, M. R. (2009). New source of muscle-derived stem cells with potential for alveolar bone reconstruction in cleft lip and\/or palate patients. Tissue Engineering. Part A, 15(2), 427\u2013435.","journal-title":"Tissue Engineering. Part A"},{"issue":"11","key":"9820_CR20","doi-asserted-by":"publisher","first-page":"2039","DOI":"10.2106\/JBJS.I.01368","volume":"92","author":"MM Murray","year":"2010","unstructured":"Murray, M. M., Magarian, E. M., Harrison, S. L., Mastrangelo, A. N., Zurakowski, D., & Fleming, B. C. (2010). The effect of skeletal maturity on functional healing of the anterior cruciate ligament. The Journal of Bone and Joint Surgery. American Volume [Internet], 92(11), 2039\u20132049. [cited 2015 Jul 20]; Available from: \n                    http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=2924734&tool=pmcentrez&rendertype=abstract\n                    \n                  .","journal-title":"The Journal of Bone and Joint Surgery. American Volume [Internet]"},{"issue":"12","key":"9820_CR21","doi-asserted-by":"publisher","first-page":"1397","DOI":"10.1016\/j.joca.2007.05.005","volume":"15","author":"MPJ Borne van den","year":"2007","unstructured":"van den Borne, M. P. J., Raijmakers, N. J. H., Vanlauwe, J., et al. (2007). International Cartilage Repair Society (ICRS) and Oswestry macroscopic cartilage evaluation scores validated for use in Autologous Chondrocyte Implantation (ACI) and microfracture. Osteoarthritis Cartilage [Internet], 15(12), 1397\u20131402. [cited 2015 Mar 1]; Available from: \n                    http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1063458407001707\n                    \n                  .","journal-title":"Osteoarthritis Cartilage [Internet]"},{"issue":"4","key":"9820_CR22","doi-asserted-by":"publisher","first-page":"595","DOI":"10.2106\/00004623-198870040-00017","volume":"70","author":"SW O\u2019Driscoll","year":"1988","unstructured":"O\u2019Driscoll, S. W., Keeley, F. W., & Salter, R. B. (1988). Durability of regenerated articular cartilage produced by free autogenous periosteal grafts in major full-thickness defects in joint surfaces under the influence of continuous passive motion. A follow-up report at one year. The Journal of Bone and Joint Surgery. American Volume, 70(4), 595\u2013606.","journal-title":"The Journal of Bone and Joint Surgery. American Volume"},{"issue":"6","key":"9820_CR23","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1159\/000321160","volume":"193","author":"J Karbanov\u00e1","year":"2011","unstructured":"Karbanov\u00e1, J., Soukup, T., Such\u00e1nek, J., Pytl\u00edk, R., Corbeil, D., & Mokr\u00fd, J. (2011). Characterization of dental pulp stem cells from impacted third molars cultured in low serum-containing medium. Cells, Tissues, Organs, 193(6), 344\u2013365.","journal-title":"Cells, Tissues, Organs"},{"issue":"6","key":"9820_CR24","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1038\/gt.2012.3","volume":"19","author":"K Ishikawa","year":"2012","unstructured":"Ishikawa, K., Tilemann, L., Ladage, D., Aguero, J., Leonardson, L., Fish, K., & Kawase, Y. (2012). Cardiac gene therapy in large animals: Bridge from bench to bedside. Gene Therapy, 19(6), 670\u2013677.","journal-title":"Gene Therapy"},{"issue":"3","key":"9820_CR25","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/s00441-002-0525-z","volume":"307","author":"J Ringe","year":"2002","unstructured":"Ringe, J., Kaps, C., Schmitt, B., B\u00fcscher, K., Bartel, J., Smolian, H., Schultz, O., Burmester, G., H\u00e4upl, T., & Sittinger, M. (2002). Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages. Cell and Tissue Research, 307(3), 321\u2013327.","journal-title":"Cell and Tissue Research"},{"issue":"4","key":"9820_CR26","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1007\/s12015-016-9659-3","volume":"12","author":"MV Pelatti","year":"2016","unstructured":"Pelatti, M. V., Gomes, J. P. A., Vieira, N. M. S., et al. (2016). Transplantation of human adipose mesenchymal stem cells in non-immunosuppressed GRMD dogs is a safe procedure. Stem Cell Reviews and Reports [Internet], 12(4), 448\u2013453. Available from: \n                    https:\/\/doi.org\/10.1007\/s12015-016-9659-3\n                    \n                  .","journal-title":"Stem Cell Reviews and Reports [Internet]"},{"issue":"3","key":"9820_CR27","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1038\/nbt.2816","volume":"32","author":"JA Ankrum","year":"2014","unstructured":"Ankrum, J. A., Ong, J. F., & Karp, J. M. (2014). Mesenchymal stem cells: Immune evasive, not immune privileged. Nature Biotechnology, 32(3), 252\u2013260.","journal-title":"Nature Biotechnology"},{"issue":"10","key":"9820_CR28","doi-asserted-by":"publisher","first-page":"3955","DOI":"10.1016\/j.transproceed.2011.08.102","volume":"43","author":"R Tang","year":"2011","unstructured":"Tang, R., & Ding, G. (2011). Swine dental pulp stem cells inhibit T-cell proliferation. Transplantation Proceedings [Internet], 43(10), 3955\u20133959. Available from: \n                    https:\/\/doi.org\/10.1016\/j.transproceed.2011.08.102\n                    \n                  .","journal-title":"Transplantation Proceedings [Internet]"},{"issue":"3\u20134","key":"9820_CR29","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1089\/ten.tea.2014.0384","volume":"21","author":"MB Fisher","year":"2015","unstructured":"Fisher, M. B., Belkin, N. S., Milby, A. H., et al. (2015). Cartilage repair and subchondral bone remodeling in response to focal lesions in a mini-pig model: implications for tissue engineering. Tissue Engineering Part A [Internet], 21(3\u20134), 850\u2013860. [cited 2015 Jul 3]; Available from: \n                    http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=4333259&tool=pmcentrez&rendertype=abstract\n                    \n                  .","journal-title":"Tissue Engineering Part A [Internet]"},{"issue":"4","key":"9820_CR30","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1089\/107632702760240616","volume":"8","author":"Y Liu","year":"2002","unstructured":"Liu, Y., Chen, F., Liu, W., Cui, L., Shang, Q., Xia, W., Wang, J., Cui, Y., Yang, G., Liu, D., Wu, J., Xu, R., Buonocore, S. D., & Cao, Y. (2002). Repairing large porcine full-thickness defects of articular cartilage using autologous chondrocyte-engineered cartilage. Tissue Engineering, 8(4), 709\u2013721.","journal-title":"Tissue Engineering"}],"container-title":["Stem Cell Reviews and Reports"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12015-018-9820-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12015-018-9820-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12015-018-9820-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T23:20:00Z","timestamp":1556925600000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12015-018-9820-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,4]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["9820"],"URL":"https:\/\/doi.org\/10.1007\/s12015-018-9820-2","relation":{},"ISSN":["1550-8943","1558-6804"],"issn-type":[{"value":"1550-8943","type":"print"},{"value":"1558-6804","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,4]]},"assertion":[{"value":"4 May 2018","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"The Ethics and Research Committee at S\u00edrio-Liban\u00eas Hospital, S\u00e3o Paulo, Brazil approved this study (protocol: CEUA-P 2015\u201308).Experimental procedures and animal care were conducted in accordance with Law 11,794\/2008 of the Brazilian Federal Constitution, which regulates and establishes \u201cProcedures for the scientific use of animals.\u201d","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research Involving Animals"}}]}}