{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T00:34:45Z","timestamp":1792974885819,"version":"4.5.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Science and Technology Innovation Commission Foundation of Shenzhen","award":["JCYJ20190808141013454"],"award-info":[{"award-number":["JCYJ20190808141013454"]}]},{"name":"Science and Technology Innovation Commission Foundation of Shenzhen","award":["JCYJ20180305124827261"],"award-info":[{"award-number":["JCYJ20180305124827261"]}]},{"DOI":"10.13039\/501100017621","name":"Shenzhen Key Laboratory Foundation","doi-asserted-by":"crossref","award":["ZDSYS20200811143757022"],"award-info":[{"award-number":["ZDSYS20200811143757022"]}],"id":[{"id":"10.13039\/501100017621","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Rev and Rep"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Partial bladder outlet obstruction (pBOO) often results in bladder tissue inflammation and remodeling. As human urine-derived stem cells (USCs) have demonstrated therapeutic benefits, we used a rat model to investigate the effect of USCs on bladder function and explore the miRNA and gene expression profiles in bladder tissue using RNA sequencing. Eighteen rats were assigned to a sham surgery group, pBOO group, and pBOO+USC group (six biweekly treatments). Routine urodynamic monitoring, analysis of detrusor muscle strips, and pathophysiology assessments were conducted. Finally, altered miRNA and mRNA expression profiles of bladder tissue were examined using RNA sequencing and bioinformatics analysis. After USC treatment, elevated bladder compliance and maximal voiding pressure, declined end filling pressure and voided volume, and improved detrusor muscle contractility and carbachol sensitivity were found. Histology and TUNEL assay revealed reduced collagen deposition and muscle cell apoptosis in bladder tissue. The differential expression of eight miRNAs was reversed by USC treatment. Two large nodes (miR-142 and miR-9a) were identified in the miRNA-gene interaction network in the USC-treated group. The Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment of multiple significant pathways, including those involved in necroptosis and cytokine-cytokine receptor interactions. This is the first study to demonstrate the protective effect of USCs on bladder function and remodeling in pBOO rats. The miRNA and mRNA expression levels differed in the bladder of pBOO rats with and without USC treatment. Although the mechanism underlying these effects has not been fully elucidated, necroptosis and cytokine-cytokine receptor interaction-related pathways may be involved.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s12015-022-10340-0","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T11:41:50Z","timestamp":1646134910000},"page":"2403-2413","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Human Urine-Derived Stem Cells Improve Partial Bladder Outlet Obstruction in Rats: Preliminary Data and microRNA-mRNA Expression Profile"],"prefix":"10.1007","volume":"18","author":[{"given":"Menjiang","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bishao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keshi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8108-4979","authenticated-orcid":false,"given":"Xiangwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,1]]},"reference":[{"issue":"3","key":"10340_CR1","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1016\/j.juro.2016.02.2958","volume":"196","author":"YH Jiang","year":"2016","unstructured":"Jiang, Y. H., Lee, C. L., & Kuo, H. C. (2016). Urothelial dysfunction, suburothelial inflammation and altered sensory protein expression in men with bladder outlet obstruction and various bladder dysfunctions: Correlation with urodynamics. Journal of Urology, 196(3), 831\u2013837.","journal-title":"Journal of Urology"},{"key":"10340_CR2","doi-asserted-by":"crossref","unstructured":"Wiafe, B., Adesida, A. B., Churchill, T., Kadam, R., Carleton, J., & Metcalfe, P. D. (2019). Mesenchymal stem cell therapy inhibited inflammatory and profibrotic pathways induced by partial bladder outlet obstruction and prevented high-pressure urine storage. Journal of Pediatric Urology, 15(3), 254.e1\u2013254.e10.","DOI":"10.1016\/j.jpurol.2019.03.003"},{"issue":"1","key":"10340_CR3","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/j.juro.2009.02.104","volume":"182","author":"M Oka","year":"2009","unstructured":"Oka, M., Fukui, T., Ueda, M., Tagaya, M., Oyama, T., & Tanaka, M. (2009). Suppression of bladder oxidative stress and inflammation by a phytotherapeutic agent in a rat model of partial bladder outlet obstruction. Journal of Urology, 182(1), 382\u2013390.","journal-title":"Journal of Urology"},{"issue":"4","key":"10340_CR4","first-page":"1876","volume":"9","author":"Q Wang","year":"2017","unstructured":"Wang, Q., Zhao, J., Wu, C., Yang, Z., Dong, X., Liu, Q., et al. (2017). Large conductance voltage and Ca(2+)-activated K(+) channels affect the physiological characteristics of human urine-derived stem cells. American Journal of Translational Research, 9(4), 1876\u20131885.","journal-title":"American Journal of Translational Research"},{"issue":"3","key":"10340_CR5","doi-asserted-by":"publisher","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","volume":"10","author":"B Langmead","year":"2009","unstructured":"Langmead, B., Trapnell, C., Pop, M., & Salzberg, S. L. (2009). Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. 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Written informed consent was obtained from all donors prior to the collection of urine samples.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}