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While luminal epithelial cells line the uterine cavity and build the embryo-maternal contact zone, glandular epithelial cells form tubular glands reaching deeply into the endometrial stroma. To facilitate investigations considering the functional and molecular differences between the two populations of epithelial cells and their contribution to reproductive processes, we aimed at establishing differentiated<jats:italic>in vitro<\/jats:italic>models of both the luminal and the glandular epithelium of the porcine endometrium using an air\u2013liquid interface (ALI) approach. We first tested if porcine luminal endometrium epithelial cells (PEEC-L) reproducibly form differentiated epithelial monolayers under ALI conditions by monitoring the morphology and the trans-epithelial electrical resistance (TEER). Subsequently, luminal (PEEC-L) and glandular epithelial cells (PEEC-G) were consecutively isolated from the endometrium of the uterine horn. Both cell types were characterized by marker gene expression analysis immediately after isolation. Cells were separately grown at the ALI and assessed by means of histomorphometry, TEER, and marker gene expression after 3\u00a0weeks of culture. PEEC-L and PEEC-G formed polarized monolayers of differentiated epithelial cells with a moderate TEER and<jats:italic>in vivo<\/jats:italic>-like morphology at the ALI. They exhibited distinct patterns of functional and cell type-specific marker gene expression after isolation and largely maintained these patterns during the culture period. The here presented cell culture procedure for PEEC-L and -G offers new opportunities to study the impact of embryonic signals, endocrine effectors, and reproductive toxins on both porcine endometrial epithelial cell types under standardized<jats:italic>in vitro<\/jats:italic>conditions.<\/jats:p><jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p><jats:p>Created with<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.BioRender.com\">BioRender.com<\/jats:ext-link>.<\/jats:p>","DOI":"10.1007\/s12015-022-10410-3","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T08:02:40Z","timestamp":1658131360000},"page":"2928-2938","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Luminal and Glandular Epithelial Cells from the Porcine Endometrium maintain Cell Type-Specific Marker Gene Expression in Air\u2013Liquid Interface Culture"],"prefix":"10.1007","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4765-1743","authenticated-orcid":false,"given":"Meret","family":"Schmidhauser","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0355-3869","authenticated-orcid":false,"given":"Susanne E.","family":"Ulbrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5790-3217","authenticated-orcid":false,"given":"Jennifer","family":"Schoen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"issue":"2","key":"10410_CR1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1600-0897.2004.00248.x","volume":"53","author":"CR Wira","year":"2005","unstructured":"Wira, C. 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