{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:24:10Z","timestamp":1780392250412,"version":"3.54.1"},"reference-count":78,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100019706","name":"Qatar Biomedical Research Institute, Hamad Bin Khalifa University","doi-asserted-by":"publisher","award":["IGP3 and QBRI-HSCI Project 1"],"award-info":[{"award-number":["IGP3 and QBRI-HSCI Project 1"]}],"id":[{"id":"10.13039\/100019706","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019687","name":"Hamad bin Khalifa University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100019687","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Rev and Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, we reported that forkhead box A2 (FOXA2) is required for the development of human pancreatic \u03b1- and \u03b2-cells. However, whether miRNAs play a role in regulating pancreatic genes during pancreatic development in the absence of FOXA2 expression is largely unknown. Here, we aimed to capture the dysregulated miRNAs and to identify their pancreatic-specific gene targets in pancreatic progenitors (PPs) derived from wild-type induced pluripotent stem cells (WT-iPSCs) and from iPSCs lacking FOXA2 (<jats:italic>FOXA2<\/jats:italic><jats:sup>\u2013\/\u2013<\/jats:sup>iPSCs). To identify differentially expressed miRNAs (DEmiRs), and genes (DEGs), two different <jats:italic>FOXA2<\/jats:italic><jats:sup>\u2013\/\u2013<\/jats:sup>iPSC lines were differentiated into PPs. <jats:italic>FOXA2<\/jats:italic><jats:sup>\u2013\/\u2013<\/jats:sup> PPs showed a significant reduction in the expression of the main PP transcription factors (TFs) in comparison to WT-PPs. RNA sequencing analysis demonstrated significant reduction in the mRNA expression of genes involved in the development and function of exocrine and endocrine pancreas. Furthermore, miRNA profiling identified 107 downregulated and 111 upregulated DEmiRs in <jats:italic>FOXA2<\/jats:italic><jats:sup>\u2013\/\u2013<\/jats:sup> PPs compared to WT-PPs. Target prediction analysis between DEmiRs and DEGs identified 92 upregulated miRNAs, predicted to target 1498 downregulated genes in <jats:italic>FOXA2<\/jats:italic><jats:sup>\u2013\/\u2013<\/jats:sup> PPs. Several important pancreatic TFs essential for pancreatic development were targeted by multiple DEmiRs. Selected DEmiRs and DEGs were further validated using RT-qPCR. Our findings revealed that FOXA2 expression is crucial for pancreatic development through regulating the expression of pancreatic endocrine and exocrine genes targeted by a set of miRNAs at the pancreatic progenitor stage. These data provide novel insights of the effect of <jats:italic>FOXA2<\/jats:italic> deficiency on miRNA-mRNA regulatory networks controlling pancreatic development and differentiation.<\/jats:p>\n              <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s12015-023-10515-3","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T08:50:30Z","timestamp":1675759830000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["iPSC-Derived Pancreatic Progenitors Lacking FOXA2 Reveal Alterations in miRNA Expression Targeting Key Pancreatic Genes"],"prefix":"10.1007","author":[{"given":"Noura","family":"Aldous","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed K.","family":"Elsayed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nehad M.","family":"Alajez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7764-6772","authenticated-orcid":false,"given":"Essam M.","family":"Abdelalim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"10515_CR1","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1111\/brv.12349","volume":"93","author":"S Al-Khawaga","year":"2018","unstructured":"Al-Khawaga, S., Memon, B., Butler, A. E., Taheri, S., Abou-Samra, A. B., & Abdelalim, E. M. (2018). Pathways governing development of stem cell-derived pancreatic \u03b2 cells: lessons from embryogenesis. Biological Reviews, 93, 364\u2013389.","journal-title":"Biological Reviews"},{"key":"10515_CR2","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1007\/s00125-008-1006-z","volume":"51","author":"BM Lyttle","year":"2008","unstructured":"Lyttle, B. M., Li, J., Krishnamurthy, M., Fellows, F., Wheeler, M. B., Goodyer, C. G., & Wang, R. (2008). Transcription factor expression in the developing human fetal endocrine pancreas. Diabetologia, 51, 1169\u20131180.","journal-title":"Diabetologia"},{"key":"10515_CR3","doi-asserted-by":"publisher","first-page":"3514","DOI":"10.2337\/db12-1479","volume":"62","author":"RE Jennings","year":"2013","unstructured":"Jennings, R. E., Berry, A. A., Kirkwood-Wilson, R., Roberts, N. A., Hearn, T., Salisbury, R. J., Blaylock, J., Hanley, K. P., & Hanley, N. A. (2013). Development of the human pancreas from Foregut to endocrine commitment. Diabetes, 62, 3514\u20133522.","journal-title":"Diabetes"},{"key":"10515_CR4","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1369\/jhc.2009.953307","volume":"57","author":"J Jeon","year":"2009","unstructured":"Jeon, J., Correa-Medina, M., Ricordi, C., Edlund, H., & Diez, J. A. (2009). Endocrine cell clustering during human pancreas development. Journal of Histochemistry & Cytochemistry, 57, 811\u2013824.","journal-title":"Journal of Histochemistry & Cytochemistry"},{"key":"10515_CR5","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.molmet.2017.03.007","volume":"6","author":"A Bastidas-Ponce","year":"2017","unstructured":"Bastidas-Ponce, A., Roscioni, S. S., Burtscher, I., Bader, E., Sterr, M., Bakhti, M., & Lickert, H. (2017). Foxa2 and Pdx1 cooperatively regulate postnatal maturation of pancreatic \u03b2-cells. Molecular Metabolism, 6, 524\u2013534.","journal-title":"Molecular Metabolism"},{"key":"10515_CR6","doi-asserted-by":"publisher","first-page":"3781","DOI":"10.1210\/en.2013-1843","volume":"155","author":"M Heddad Masson","year":"2014","unstructured":"Heddad Masson, M., Poisson, C., Gu\u00e9rardel, A., Mamin, A., Philippe, J., & Gosmain, Y. (2014). Foxa1 and Foxa2 regulate \u03b1-cell differentiation, glucagon biosynthesis, and secretion. Endocrinology, 155, 3781\u20133792.","journal-title":"Endocrinology"},{"key":"10515_CR7","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1038\/s41419-021-03390-8","volume":"12","author":"AK Elsayed","year":"2021","unstructured":"Elsayed, A. K., Younis, I., Ali, G., Hussain, K., & Abdelalim, E. M. (2021). Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency. Cell Death & Disease, 12, 103.","journal-title":"Cell Death & Disease"},{"key":"10515_CR8","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1038\/s41419-022-05158-0","volume":"13","author":"M Aghadi","year":"2022","unstructured":"Aghadi, M., Elgendy, R., & Abdelalim, E. M. (2022). Loss of FOXA2 induces ER stress and hepatic steatosis and alters developmental gene expression in human iPSC-derived hepatocytes. Cell Death & Disease, 13, 713.","journal-title":"Cell Death & Disease"},{"key":"10515_CR9","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/j.celrep.2019.06.034","volume":"28","author":"K Lee","year":"2019","unstructured":"Lee, K., Cho, H., Rickert, R. W., Li, Q. V., Pulecio, J., Leslie, C. S., & Huangfu, D. (2019). FOXA2 is required for enhancer priming during pancreatic differentiation. Cell Reports, 28, 382-393e7.","journal-title":"Cell Reports"},{"key":"10515_CR10","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1111\/pedi.12814","volume":"20","author":"C Stekelenburg","year":"2019","unstructured":"Stekelenburg, C., Gerster, K., Blouin, J. L., Lang-Muritano, M., Guipponi, M., Santoni, F., & Schwitzgebel, V. M. (2019). Exome sequencing identifies a de novo FOXA2 variant in a patient with syndromic diabetes. Pediatric Diabetes, 20, 366\u2013369.","journal-title":"Pediatric Diabetes"},{"key":"10515_CR11","doi-asserted-by":"crossref","unstructured":"Gaulton, K. J., Ferreira, T., Lee, Y., Raimondo, A., M\u00e4gi, R., Reschen, M. E., Mahajan, A., Locke, A., Rayner, N. W., Robertson, N., Scott, R. A., Prokopenko, I., Scott, L. J., Green, T., Sparso, T., Thuillier, D., Yengo, L., Grallert, H., Wahl, S., Fr\u00e5nberg, M., Strawbridge, R. J., Kestler, H., Chheda, H., Eisele, L., Gustafsson, S., Steinthorsdottir, V., Thorleifsson, G., Qi, L., Karssen, L. C., van Leeuwen, E. M., Willems, S. M., Li, M., Chen, H., Fuchsberger, C., Kwan, P., Ma, C., Linderman, M., Lu, Y., Thomsen, S. K., Rundle, J. K., Beer, N. L., van de, M., Bunt, Chalisey, A., Kang, H. M., Voight, B. F., Abecasis, G. R., Almgren, P., Baldassarre, D., Balkau, B., Benediktsson, R., Bl\u00fcher, M., Boeing, H., Bonnycastle, L. L., Bottinger, E. P., Burtt, N. P., Carey, J., Charpentier, G., Chines, P. S., Cornelis, M. C., Couper, D. J., Crenshaw, A. T., van Dam, R. M., Doney, A. S., Dorkhan, M., Edkins, S., Eriksson, J. G., Esko, T., Eury, E., Fadista, J., Flannick, J., Fontanillas, P., Fox, C., Franks, P. W., Gertow, K., Gieger, C., Gigante, B., Gottesman, O., Grant, G. B., Grarup, N., Groves, C. J., Hassinen, M., Have, C. T., Herder, C., Holmen, O. L., Hreidarsson, A. B., Humphries, S. E., Hunter, D. J., Jackson, A. U., Jonsson, A. and, ME J\u00f8rgensen and T J\u00f8rgensen and, Kao, W. H., Kerrison, N. D., Kinnunen, L., Klopp, N., Kong, A., Kovacs, P., Kraft, P., Kravic, J., Langford, C., Leander, K., Liang, L., Lichtner, P., Lindgren, C. M., Lindholm, E., Linneberg, A., Liu, C. T., Lobbens, S., Luan, J., Lyssenko, V., M\u00e4nnist\u00f6, S., McLeod, O., Meyer, J., Mihailov, E., Mirza, G., M\u00fchleisen, T. W., M\u00fcller-Nurasyid, M., Navarro, C., N\u00f6then, M. M., Oskolkov, N. N., Owen, K. R., Palli, D., Pechlivanis, S., Peltonen, L., Perry, J. R., Platou, C. G., Roden, M., Ruderfer, D., Rybin, D., van der Schouw, Y. T., Sennblad, B., Sigur\u00f0sson, G., Stan\u010d\u00e1kov\u00e1, A., Steinbach, G., Storm, P., Strauch, K., Stringham, H. M., Sun, Q., Thorand, B., Tikkanen, E., Tonjes, A., Trakalo, J., Tremoli, E., Tuomi, T., Wennauer, R., Wiltshire, S., Wood, A. R., Zeggini, E., Dunham, I., Birney, E., Pasquali, L., Ferrer, J., Loos, R. J., Dupuis, J., Florez, J. C., Boerwinkle, E., Pankow, J. S., van Duijn, C., Sijbrands, E., Meigs, J. B., Hu, F. B., Thorsteinsdottir, U., Stefansson, K., Lakka, T. A., Rauramaa, R., Stumvoll, M., Pedersen, N. L., Lind, L., Keinanen-Kiukaanniemi, S. M., Korpi-Hy\u00f6v\u00e4lti, E., Saaristo, T. E., Saltevo, J., Kuusisto, J., Laakso, M., Metspalu, A., Erbel, R., J\u00f6cke, K. H., Moebus, S., Ripatti, S., Salomaa, V., Ingelsson, E., Boehm, B. O., Bergman, R. N., Collins, F. S., Mohlke, K. L., Koistinen, H., Tuomilehto, J., Hveem, K., Nj\u00f8lstad, I., Deloukas, P., Donnelly, P. J., Frayling, T. M., Hattersley, A. T., de Faire, U., Hamsten, A., Illig, T., Peters, A., Cauchi, S., Sladek, R., Froguel, P., Hansen, T., Pedersen, O., Morris, A. D., Palmer, C. N., Kathiresan, S., Melander, O., Nilsson, P. M., Groop, L. C., Barroso, I., Langenberg, C., Wareham, N. J., O\u2019Callaghan, C. A., Gloyn, A. L., Altshuler, D., Boehnke, M., Teslovich, T. M., McCarthy, M. I., & Morris, A. P. (2015). Genetic fine mapping and genomic annotation defines causal mechanisms at type 2 diabetes susceptibility loci. Nat Genet 47:1415-25.","DOI":"10.1038\/ng.3437"},{"key":"10515_CR12","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1016\/j.molmet.2017.06.020","volume":"6","author":"MP LaPierre","year":"2017","unstructured":"LaPierre, M. P., & Stoffel, M. (2017). MicroRNAs as stress regulators in pancreatic beta cells and diabetes. Mol Metab, 6, 1010\u20131023.","journal-title":"Mol Metab"},{"key":"10515_CR13","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1038\/nature03076","volume":"432","author":"MN Poy","year":"2004","unstructured":"Poy, M. N., Eliasson, L., Krutzfeldt, J., Kuwajima, S., Ma, X., Macdonald, P. E., Pfeffer, S., Tuschl, T., Rajewsky, N., Rorsman, P., & Stoffel, M. (2004). A pancreatic islet-specific microRNA regulates insulin secretion. Nature, 432, 226\u2013230.","journal-title":"Nature"},{"key":"10515_CR14","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1038\/nature09267","volume":"466","author":"H Guo","year":"2010","unstructured":"Guo, H., Ingolia, N. T., Weissman, J. S., & Bartel, D. P. (2010). Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature, 466, 835\u2013840.","journal-title":"Nature"},{"key":"10515_CR15","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1038\/nature03315","volume":"433","author":"LP Lim","year":"2005","unstructured":"Lim, L. P., Lau, N. C., Garrett-Engele, P., Grimson, A., Schelter, J. M., Castle, J., Bartel, D. P., Linsley, P. S., & Johnson, J. M. (2005). Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature, 433, 769\u2013773.","journal-title":"Nature"},{"key":"10515_CR16","doi-asserted-by":"publisher","first-page":"1931","DOI":"10.1126\/science.1149460","volume":"318","author":"S Vasudevan","year":"2007","unstructured":"Vasudevan, S., Tong, Y., & Steitz, J. A. (2007). Switching from repression to activation: microRNAs can up-regulate translation. Science, 318, 1931\u20131934.","journal-title":"Science"},{"key":"10515_CR17","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.tcb.2014.11.004","volume":"25","author":"JA Vidigal","year":"2015","unstructured":"Vidigal, J. A., & Ventura, A. (2015). The biological functions of miRNAs: lessons from in vivo studies. Trends In Cell Biology, 25, 137\u2013147.","journal-title":"Trends In Cell Biology"},{"key":"10515_CR18","doi-asserted-by":"crossref","unstructured":"Poy, M. N., Hausser, J., Trajkovski, M., Braun, M., Collins, S., Rorsman, P., Zavolan, M., & Stoffel, M. (2009). miR-375 maintains normal pancreatic \u03b1- and \u03b2-cell mass. Proceedings of the National Academy of Sciences, 106, 5813\u20135818.","DOI":"10.1073\/pnas.0810550106"},{"key":"10515_CR19","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/nm0106-36","volume":"12","author":"BR Gauthier","year":"2006","unstructured":"Gauthier, B. R., & Wollheim, C. B. (2006). MicroRNAs: \u2018ribo-regulators\u2019 of glucose homeostasis. Nature Medicine, 12, 36\u201338.","journal-title":"Nature Medicine"},{"key":"10515_CR20","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1038\/emboj.2010.361","volume":"30","author":"T Melkman-Zehavi","year":"2011","unstructured":"Melkman-Zehavi, T., Oren, R., Kredo-Russo, S., Shapira, T., Mandelbaum, A. D., Rivkin, N., Nir, T., Lennox, K. A., Behlke, M. A., Dor, Y., & Hornstein, E. (2011). miRNAs control insulin content in pancreatic \u03b2-cells via downregulation of transcriptional repressors. The EMBO Journal, 30, 835\u2013845.","journal-title":"The EMBO Journal"},{"key":"10515_CR21","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/j.bbagrm.2015.12.001","volume":"1859","author":"C Bai","year":"2016","unstructured":"Bai, C., Li, X., Gao, Y., Wang, K., Fan, Y., Zhang, S., Ma, Y., & Guan, W. (2016). Role of microRNA-21 in the formation of insulin-producing cells from pancreatic progenitor cells. Biochimica Et Biophysica Acta, 1859, 280\u2013293.","journal-title":"Biochimica Et Biophysica Acta"},{"key":"10515_CR22","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1261\/rna.1211209","volume":"15","author":"X Tang","year":"2009","unstructured":"Tang, X., Muniappan, L., Tang, G., & \u00d6zcan, S. (2009). Identification of glucose-regulated miRNAs from pancreatic \u03b2 cells reveals a role for miR-30d in insulin transcription. RNA, 15, 287\u2013293.","journal-title":"RNA"},{"key":"10515_CR23","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1002\/jcb.26203","volume":"119","author":"E Coskun","year":"2018","unstructured":"Coskun, E., Ercin, M., & Gezginci-Oktayoglu, S. (2018). The role of epigenetic regulation and pluripotency-related MicroRNAs in differentiation of pancreatic stem cells to Beta cells. Journal of Cellular Biochemistry, 119, 455\u2013467.","journal-title":"Journal of Cellular Biochemistry"},{"key":"10515_CR24","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1515\/BC.2008.026","volume":"389","author":"P Lovis","year":"2008","unstructured":"Lovis, P., Gattesco, S., & Regazzi, R. (2008). Regulation of the expression of components of the exocytotic machinery of insulin-secreting cells by microRNAs. Biological Chemistry, 389, 305\u2013312.","journal-title":"Biological Chemistry"},{"key":"10515_CR25","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1186\/1471-2164-11-509","volume":"11","author":"S Rosero","year":"2010","unstructured":"Rosero, S., Bravo-Egana, V., Jiang, Z., Khuri, S., Tsinoremas, N., Klein, D., Sabates, E., Correa-Medina, M., Ricordi, C., Dom\u00ednguez-Bendala, J., Diez, J., & Pastori, R. L. (2010). MicroRNA signature of the human developing pancreas. BMC Genomics, 11, 509.","journal-title":"BMC Genomics"},{"key":"10515_CR26","doi-asserted-by":"publisher","first-page":"26932","DOI":"10.1074\/jbc.M601225200","volume":"281","author":"V Plaisance","year":"2006","unstructured":"Plaisance, V., Abderrahmani, A., Perret-Menoud, V., Jacquemin, P., Lemaigre, F., & Regazzi, R. (2006). MicroRNA-9 controls the expression of Granuphilin\/Slp4 and the secretory response of insulin-producing cells *. Journal of Biological Chemistry, 281, 26932\u201326942.","journal-title":"Journal of Biological Chemistry"},{"key":"10515_CR27","first-page":"555","volume":"14","author":"A Shaer","year":"2016","unstructured":"Shaer, A., Azarpira, N., Karimi, M. H., Soleimani, M., & Dehghan, S. (2016). Differentiation of Human-Induced pluripotent stem cells into insulin-producing clusters by MicroRNA-7. Experimental and Clinical Transplantation, 14, 555\u2013563.","journal-title":"Experimental and Clinical Transplantation"},{"key":"10515_CR28","first-page":"226","volume":"7","author":"A Martinez-Sanchez","year":"2016","unstructured":"Martinez-Sanchez, A., Rutter, G. A., & Latreille, M. (2016). MiRNAs in \u03b2-cell development, identity, and disease. Frontiers in Genetics, 7, 226.","journal-title":"Frontiers in Genetics"},{"key":"10515_CR29","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.tem.2014.01.003","volume":"25","author":"H Kaspi","year":"2014","unstructured":"Kaspi, H., Pasvolsky, R., & Hornstein, E. (2014). Could microRNAs contribute to the maintenance of \u03b2 cell identity? Trends in Endocrinology & Metabolism, 25, 285\u2013292.","journal-title":"Trends in Endocrinology & Metabolism"},{"key":"10515_CR30","doi-asserted-by":"publisher","first-page":"2203","DOI":"10.1007\/s00125-013-2993-y","volume":"56","author":"V Nesca","year":"2013","unstructured":"Nesca, V., Guay, C., Jacovetti, C., Menoud, V., Peyot, M. L., Laybutt, D. R., Prentki, M., & Regazzi, R. (2013). Identification of particular groups of microRNAs that positively or negatively impact on beta cell function in obese models of type 2 diabetes. Diabetologia, 56, 2203\u20132212.","journal-title":"Diabetologia"},{"key":"10515_CR31","doi-asserted-by":"publisher","first-page":"2938","DOI":"10.2337\/db07-0175","volume":"56","author":"FC Lynn","year":"2007","unstructured":"Lynn, F. C., Skewes-Cox, P., Kosaka, Y., McManus, M. T., Harfe, B. D., & German, M. S. (2007). MicroRNA expression is required for pancreatic islet cell genesis in the mouse. Diabetes, 56, 2938\u20132945.","journal-title":"Diabetes"},{"key":"10515_CR32","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1089\/scd.2019.0150","volume":"29","author":"G Ali","year":"2020","unstructured":"Ali, G., Elsayed, A. K., Nandakumar, M., Bashir, M., Younis, I., Abu Aqel, Y., Memon, B., Temanni, R., Abubaker, F., Taheri, S., & Abdelalim, E. M. (2020). Keratinocytes derived from patient-specific Induced pluripotent stem cells recapitulate the genetic signature of Psoriasis Disease. Stem Cells And Development, 29, 383\u2013400.","journal-title":"Stem Cells And Development"},{"key":"10515_CR33","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s13287-017-0759-z","volume":"9","author":"B Memon","year":"2018","unstructured":"Memon, B., Karam, M., Al-Khawaga, S., & Abdelalim, E. M. (2018). Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Research & Therapy, 9, 15.","journal-title":"Stem Cell Research & Therapy"},{"key":"10515_CR34","doi-asserted-by":"publisher","unstructured":"Memon, B., & Abdelalim, E. M. (2021). Differentiation of human pluripotent stem cells into pancreatic beta-cell Precursors in a 2D culture system.\u00a0Journal of Visualized Experiments. https:\/\/doi.org\/10.3791\/63298","DOI":"10.3791\/63298"},{"key":"10515_CR35","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1007\/7651_2020_323","volume":"2454","author":"B Memon","year":"2022","unstructured":"Memon, B., & Abdelalim, E. M. (2022). Highly efficient differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Methods in Molecular Biology, 2454, 351\u2013363.","journal-title":"Methods in Molecular Biology"},{"key":"10515_CR36","doi-asserted-by":"crossref","first-page":"e3400","DOI":"10.1002\/dmrr.3400","volume":"37","author":"B Memon","year":"2020","unstructured":"Memon, B., Younis, I., Abubaker, F., & Abdelalim, E. M. (2020). PDX1\u2013\/NKX6.1 + progenitors derived from human pluripotent stem cells as a novel source of insulin-secreting cells. Diabetes\/Metabolism Research and Reviews, 37, e3400.","journal-title":"Diabetes\/Metabolism Research and Reviews"},{"key":"10515_CR37","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","volume":"4","author":"DW Huang","year":"2009","unstructured":"Huang, D. W., Sherman, B. T., & Lempicki, R. A. (2009). Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature Protocols, 4, 44\u201357.","journal-title":"Nature Protocols"},{"key":"10515_CR38","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1038\/386399a0","volume":"386","author":"B Sosa-Pineda","year":"1997","unstructured":"Sosa-Pineda, B., Chowdhury, K., Torres, M., Oliver, G., & Gruss, P. (1997). The Pax4 gene is essential for differentiation of insulin-producing beta cells in the mammalian pancreas. Nature, 386, 399\u2013402.","journal-title":"Nature"},{"key":"10515_CR39","doi-asserted-by":"publisher","first-page":"2969","DOI":"10.1242\/dev.01870","volume":"132","author":"P Collombat","year":"2005","unstructured":"Collombat, P., Hecksher-S\u00f8rensen, J., Broccoli, V., Krull, J., Ponte, I., Mundiger, T., Smith, J., Gruss, P., Serup, P., & Mansouri, A. (2005). The simultaneous loss of Arx and Pax4 genes promotes a somatostatin-producing cell fate specification at the expense of the alpha- and beta-cell lineages in the mouse endocrine pancreas. Development, 132, 2969\u20132980.","journal-title":"Development"},{"key":"10515_CR40","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","volume":"136","author":"DP Bartel","year":"2009","unstructured":"Bartel, D. P. (2009). MicroRNAs: target recognition and regulatory functions. Cell, 136, 215\u2013233.","journal-title":"Cell"},{"key":"10515_CR41","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1089\/scd.2014.0331","volume":"24","author":"D Hamam","year":"2015","unstructured":"Hamam, D., Ali, D., Kassem, M., Aldahmash, A., & Alajez, N. M. (2015). microRNAs as regulators of adipogenic differentiation of mesenchymal stem cells. Stem Cells and Development, 24, 417\u2013425.","journal-title":"Stem Cells and Development"},{"key":"10515_CR42","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1186\/s13287-020-01977-0","volume":"11","author":"II Aigha","year":"2020","unstructured":"Aigha, I. I., & Abdelalim, E. M. (2020). NKX6.1 transcription factor: a crucial regulator of pancreatic \u03b2 cell development, identity, and proliferation. Stem Cell Research & Therapy, 11, 459.","journal-title":"Stem Cell Research & Therapy"},{"key":"10515_CR43","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1186\/s13287-018-0834-0","volume":"9","author":"II Aigha","year":"2018","unstructured":"Aigha, I. I., Memon, B., Elsayed, A. K., & Abdelalim, E. M. (2018). Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors. Stem Cell Research & Therapy, 9, 83.","journal-title":"Stem Cell Research & Therapy"},{"key":"10515_CR44","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.bbrc.2010.11.024","volume":"404","author":"C Bolmeson","year":"2011","unstructured":"Bolmeson, C., Esguerra, J. L., Salehi, A., Speidel, D., Eliasson, L., & Cilio, C. M. (2011). Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects. Biochemical and Biophysical Research Communications, 404, 16\u201322.","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"10515_CR45","doi-asserted-by":"publisher","first-page":"2587","DOI":"10.1096\/fj.201701100R","volume":"32","author":"A Martinez-Sanchez","year":"2018","unstructured":"Martinez-Sanchez, A., Nguyen-Tu, M. S., Cebola, I., Yavari, A., Marchetti, P., Piemonti, L., de Koning, E., Shapiro, A. M. J., Johnson, P., Sakamoto, K., Smith, D. M., Leclerc, I., Ashrafian, H., Ferrer, J., & Rutter, G. A. (2018). MiR-184 expression is regulated by AMPK in pancreatic islets. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, 32, 2587\u20132600.","journal-title":"FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology"},{"key":"10515_CR46","doi-asserted-by":"publisher","unstructured":"Morita, S., Horii, T., Kimura, M., & Hatada, I. J. P. (2013). MiR-184 regulates insulin secretion through repression of Slc25a22.\u00a0PeerJ, 1, e162. https:\/\/doi.org\/10.7717\/peerj.162","DOI":"10.7717\/peerj.162"},{"key":"10515_CR47","doi-asserted-by":"publisher","first-page":"1865","DOI":"10.1073\/pnas.0609217104","volume":"104","author":"PA Seymour","year":"2007","unstructured":"Seymour, P. A., Freude, K. K., Tran, M. N., Mayes, E. E., Jensen, J., Kist, R., Scherer, G., & Sander, M. (2007). SOX9 is required for maintenance of the pancreatic progenitor cell pool. Proceedings of the National Academy of Sciences of the United States of America, 104, 1865\u20131870.","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10515_CR48","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1159\/000109603","volume":"12","author":"MA Maestro","year":"2007","unstructured":"Maestro, M. A., Cardalda, C., Boj, S. F., Luco, R. F., Servitja, J. M., & Ferrer, J. (2007). Distinct roles of HNF1beta, HNF1alpha, and HNF4alpha in regulating pancreas development, beta-cell function and growth. Endocrine Development, 12, 33\u201345.","journal-title":"Endocrine Development"},{"key":"10515_CR49","doi-asserted-by":"publisher","first-page":"2459","DOI":"10.1007\/s00018-020-03710-9","volume":"78","author":"EM Abdelalim","year":"2021","unstructured":"Abdelalim, E. M. (2021). Modeling different types of diabetes using human pluripotent stem cells. Cellular and Molecular Life Sciences, 78, 2459\u20132483.","journal-title":"Cellular and Molecular Life Sciences"},{"key":"10515_CR50","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1242\/dev.110759","volume":"142","author":"MG De Vas","year":"2015","unstructured":"De Vas, M. G., Kopp, J. L., Heliot, C., Sander, M., Cereghini, S., & Haumaitre, C. (2015). Hnf1b controls pancreas morphogenesis and the generation of Ngn3 + endocrine progenitors. Development, 142, 871\u2013882.","journal-title":"Development"},{"key":"10515_CR51","doi-asserted-by":"publisher","first-page":"2595","DOI":"10.1007\/s00125-011-2255-9","volume":"54","author":"Y Yang","year":"2011","unstructured":"Yang, Y., BH Chang, V., Yechoor, W., Chen, L., Li, M. J., Tsai, & Chan, L. (2011). The Kr\u00fcppel-like zinc finger protein GLIS3 transactivates neurogenin 3 for proper fetal pancreatic islet differentiation in mice. Diabetologia, 54, 2595\u20132605.","journal-title":"Diabetologia"},{"key":"10515_CR52","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1111\/j.1742-4658.2011.08042.x","volume":"278","author":"D Ramachandran","year":"2011","unstructured":"Ramachandran, D., Roy, U., Garg, S., Ghosh, S., Pathak, S., & Kolthur-Seetharam, U. (2011). Sirt1 and mir-9 expression is regulated during glucose-stimulated insulin secretion in pancreatic \u03b2-islets. The FEBS Journal, 278, 1167\u20131174.","journal-title":"The FEBS Journal"},{"key":"10515_CR53","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1007\/s00592-014-0675-y","volume":"52","author":"G Sebastiani","year":"2015","unstructured":"Sebastiani, G., Po, A., Miele, E., Ventriglia, G., Ceccarelli, E., Bugliani, M., Marselli, L., Marchetti, P., Gulino, A., Ferretti, E., & Dotta, F. (2015). MicroRNA-124a is hyperexpressed in type 2 diabetic human pancreatic islets and negatively regulates insulin secretion. Acta Diabetologica, 52, 523\u2013530.","journal-title":"Acta Diabetologica"},{"key":"10515_CR54","doi-asserted-by":"publisher","first-page":"19575","DOI":"10.1074\/jbc.M611841200","volume":"282","author":"N Baroukh","year":"2007","unstructured":"Baroukh, N., Ravier, M. A., Loder, M. K., Hill, E. V., Bounacer, A., Scharfmann, R., Rutter, G. A., & Van Obberghen, E. (2007). MicroRNA-124a regulates Foxa2 expression and Intracellular Signaling in pancreatic \u03b2-Cell Lines*. Journal of Biological Chemistry, 282, 19575\u201319588.","journal-title":"Journal of Biological Chemistry"},{"key":"10515_CR55","doi-asserted-by":"publisher","unstructured":"Ramond, C., Beydag-Tas\u00f6z, B. S., Azad, A., van de Bunt, M., Petersen, M. B. K., Beer, N. L., Glaser, N., Berthault, C., Gloyn, A. L., Hansson, M., McCarthy, M. I., Honor\u00e9, C., Grapin-Botton, A., & Scharfmann, R. (2018). Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling. Development, 145(16), dev165480. https:\/\/doi.org\/10.1242\/dev.165480","DOI":"10.1242\/dev.165480"},{"key":"10515_CR56","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1038\/mp.2015.101","volume":"21","author":"Z Xu","year":"2016","unstructured":"Xu, Z., Jiang, H., Zhong, P., Yan, Z., Chen, S., & Feng, J. (2016). Direct conversion of human fibroblasts to induced serotonergic neurons. Molecular Psychiatry, 21, 62\u201370.","journal-title":"Molecular Psychiatry"},{"key":"10515_CR57","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1007\/s12020-022-03016-9","volume":"76","author":"Z Shen","year":"2022","unstructured":"Shen, Z., Yu, Y., Yang, Y., Xiao, X., Sun, T., Chang, X., Tang, W., Zhu, Y., & Han, X. (2022). miR-25 and miR-92b regulate insulin biosynthesis and pancreatic \u03b2-cell apoptosis. Endocrine, 76, 526\u2013535.","journal-title":"Endocrine"},{"key":"10515_CR58","doi-asserted-by":"publisher","first-page":"1365","DOI":"10.4161\/rna.25557","volume":"10","author":"D Setyowati Karolina","year":"2013","unstructured":"Setyowati Karolina, D., Sepramaniam, S., Tan, H. Z., Armugam, A., & Jeyaseelan, K. (2013). miR-25 and miR-92a regulate insulin I biosynthesis in rats. RNA Biology, 10, 1365\u20131378.","journal-title":"RNA Biology"},{"key":"10515_CR59","doi-asserted-by":"publisher","first-page":"15462","DOI":"10.4238\/2015.November.30.24","volume":"14","author":"XY Chen","year":"2015","unstructured":"Chen, X. Y., Li, G. M., Dong, Q., & Peng, H. (2015). MiR-577 inhibits pancreatic \u03b2-cell function and survival by targeting fibroblast growth factor 21 (FGF-21) in pediatric diabetes. Genetics and Molecular Research, 14, 15462\u201315470.","journal-title":"Genetics and Molecular Research"},{"key":"10515_CR60","doi-asserted-by":"publisher","first-page":"2470","DOI":"10.2337\/db05-1435","volume":"55","author":"W Wente","year":"2006","unstructured":"Wente, W., Efanov, A. M., Brenner, M., Kharitonenkov, A., K\u00f6ster, A., Sandusky, G. E., Sewing, S., Treinies, I., Zitzer, H., & Gromada, J. (2006). Fibroblast growth factor-21 improves pancreatic beta-cell function and survival by activation of extracellular signal-regulated kinase 1\/2 and akt signaling pathways. Diabetes, 55, 2470\u20132478.","journal-title":"Diabetes"},{"key":"10515_CR61","doi-asserted-by":"publisher","first-page":"14051","DOI":"10.1038\/s41598-017-13622-7","volume":"7","author":"I Marzinotto","year":"2017","unstructured":"Marzinotto, I., Pellegrini, S., Brigatti, C., Nano, R., Melzi, R., Mercalli, A., Liberati, D., Sordi, V., Ferrari, M., Falconi, M., Doglioni, C., Ravassard, P., Piemonti, L., & Lampasona, V. (2017). miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA. Scientific Reports, 7, 14051.","journal-title":"Scientific Reports"},{"key":"10515_CR62","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1038\/nm.3287","volume":"19","author":"G Xu","year":"2013","unstructured":"Xu, G., Chen, J., Jing, G., & Shalev, A. (2013). Thioredoxin-interacting protein regulates insulin transcription through microRNA-204. Nature Medicine, 19, 1141\u20131146.","journal-title":"Nature Medicine"},{"key":"10515_CR63","doi-asserted-by":"publisher","unstructured":"Tian, X. Y., Ma, S., Tse, G., Wong, W. T., & Huang, Y. (2018). Uncoupling protein 2 in cardiovascular health and disease.\u00a0Front Physiology, 9, 1060. https:\/\/doi.org\/10.3389\/fphys.2018.01060","DOI":"10.3389\/fphys.2018.01060"},{"key":"10515_CR64","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.diabres.2010.11.006","volume":"91","author":"LL Sun","year":"2011","unstructured":"Sun, L. L., Jiang, B. G., Li, W. T., Zou, J. J., Shi, Y. Q., & Liu, Z. M. (2011). MicroRNA-15a positively regulates insulin synthesis by inhibiting uncoupling protein-2 expression. Diabetes Research and Clinical Practice, 91, 94\u2013100.","journal-title":"Diabetes Research and Clinical Practice"},{"key":"10515_CR65","doi-asserted-by":"publisher","first-page":"978","DOI":"10.2337\/db09-0881","volume":"59","author":"E Roggli","year":"2010","unstructured":"Roggli, E., Britan, A., Gattesco, S., Lin-Marq, N., Abderrahmani, A., Meda, P., & Regazzi, R. (2010). Involvement of microRNAs in the cytotoxic effects exerted by proinflammatory cytokines on pancreatic beta-cells. Diabetes, 59, 978\u2013986.","journal-title":"Diabetes"},{"key":"10515_CR66","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.drudis.2018.01.004","volume":"23","author":"FAA Kwa","year":"2018","unstructured":"Kwa, F. A. A., & Jackson, D. E. (2018). Manipulating the epigenome for the treatment of disorders with thrombotic complications. Drug Discovery Today, 23, 719\u2013726.","journal-title":"Drug Discovery Today"},{"key":"10515_CR67","first-page":"4192","volume":"76","author":"S Sato","year":"2016","unstructured":"Sato, S., Katsushima, K., Shinjo, K., Hatanaka, A., Ohka, F., Suzuki, S., Naiki-Ito, A., Soga, N., Takahashi, S., & Kondo, Y. (2016). Histone deacetylase inhibition in prostate Cancer triggers miR-320\u2013Mediated suppression of the androgen receptor. Cancer Research, 76, 4192\u20134204.","journal-title":"Cancer Research"},{"key":"10515_CR68","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/1479-5876-9-30","volume":"9","author":"K Matsushima","year":"2011","unstructured":"Matsushima, K., Isomoto, H., Yamaguchi, N., Inoue, N., Machida, H., Nakayama, T., Hayashi, T., Kunizaki, M., Hidaka, S., Nagayasu, T., Nakashima, M., Ujifuku, K., Mitsutake, N., Ohtsuru, A., Yamashita, S., Korpal, M., Kang, Y., Gregory, P. A., Goodall, G. J., \u2026 Nakao, K. (2011). MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells. Journal of Translational Medicine, 9, 30.","journal-title":"Journal of Translational Medicine"},{"key":"10515_CR69","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1038\/ng1725","volume":"38","author":"JF Chen","year":"2006","unstructured":"Chen, J. F., Mandel, E. M., Thomson, J. M., Wu, Q., Callis, T. E., Hammond, S. M., Conlon, F. L., & Wang, D. Z. (2006). The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nature Genetics, 38, 228\u2013233.","journal-title":"Nature Genetics"},{"key":"10515_CR70","doi-asserted-by":"publisher","first-page":"3907","DOI":"10.1038\/s41467-022-31611-x","volume":"13","author":"J Li","year":"2022","unstructured":"Li, J., Wu, X., Ke, J., Lee, M., Lan, Q., Li, J., Yu, J., Huang, Y., Sun, D. Q., & Xie, R. (2022). TET1 dioxygenase is required for FOXA2-associated chromatin remodeling in pancreatic beta-cell differentiation. Nature Communications, 13, 3907.","journal-title":"Nature Communications"},{"key":"10515_CR71","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.cbpa.2019.01.024","volume":"51","author":"Q Yao","year":"2019","unstructured":"Yao, Q., Chen, Y., & Zhou, X. (2019). The roles of microRNAs in epigenetic regulation. Current Opinion in Chemical Biology, 51, 11\u201317.","journal-title":"Current Opinion in Chemical Biology"},{"key":"10515_CR72","doi-asserted-by":"publisher","unstructured":"Dai, E., Yu, X., Zhang, Y., Meng, F., Wang, S., Liu, X., Liu, D., Wang, J., Li, X., & Jiang, W. (2014). EpimiR: a database of curated mutual regulation between miRNAs and epigenetic modifications. Database (Oxford),\u00a02014, bau023. https:\/\/doi.org\/10.1093\/database\/bau023","DOI":"10.1093\/database\/bau023"},{"key":"10515_CR73","first-page":"5","volume":"1","author":"M Kim","year":"2019","unstructured":"Kim, M., & Zhang, X. (2019). The profiling and role of miRNAs in diabetes Mellitus. Journal of Diabetes and Clinical Research, 1, 5\u201323.","journal-title":"Journal of Diabetes and Clinical Research"},{"key":"10515_CR74","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/s12933-019-0918-x","volume":"18","author":"J Pordzik","year":"2019","unstructured":"Pordzik, J., Jakubik, D., Jarosz-Popek, J., Wicik, Z., Eyileten, C., Rosa, S. D., Indolfi, C., Siller-Matula, J. M., Czajka, P., & Postula, M. (2019). Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: bioinformatic analysis and review. Cardiovascular Diabetology, 18, 113.","journal-title":"Cardiovascular Diabetology"},{"key":"10515_CR75","doi-asserted-by":"publisher","unstructured":"Smolarz, B., Durczy\u0144ski, A., Romanowicz, H., & Hogendorf, P. (2021). The role of microRNA in pancreatic cancer. Biomedicines, 9(10), 1322. https:\/\/doi.org\/10.3390\/biomedicines9101322","DOI":"10.3390\/biomedicines9101322"},{"key":"10515_CR76","doi-asserted-by":"publisher","first-page":"1832","DOI":"10.1093\/carcin\/bgr223","volume":"32","author":"SK Srivastava","year":"2011","unstructured":"Srivastava, S. K., Bhardwaj, A., Singh, S., Arora, S., Wang, B., Grizzle, W. E., & Singh, A. P. (2011). MicroRNA-150 directly targets MUC4 and suppresses growth and malignant behavior of pancreatic cancer cells. Carcinogenesis, 32, 1832\u20131839.","journal-title":"Carcinogenesis"},{"key":"10515_CR77","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.addr.2015.05.014","volume":"88","author":"B Peng","year":"2015","unstructured":"Peng, B., Chen, Y., & Leong, K. W. (2015). MicroRNA delivery for regenerative medicine. Advanced Drug Delivery Reviews, 88, 108\u2013122.","journal-title":"Advanced Drug Delivery Reviews"},{"key":"10515_CR78","doi-asserted-by":"publisher","first-page":"2629","DOI":"10.1016\/j.ajpath.2015.04.001","volume":"185","author":"CK Sen","year":"2015","unstructured":"Sen, C. K., & Ghatak, S. (2015). miRNA control of tissue repair and regeneration. American Journal of Pathology, 185, 2629\u20132640.","journal-title":"American Journal of Pathology"}],"container-title":["Stem Cell Reviews and Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12015-023-10515-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12015-023-10515-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12015-023-10515-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T13:48:24Z","timestamp":1720792104000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12015-023-10515-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,7]]},"references-count":78,"alternative-id":["10515"],"URL":"https:\/\/doi.org\/10.1007\/s12015-023-10515-3","relation":{},"ISSN":["2629-3269","2629-3277"],"issn-type":[{"value":"2629-3269","type":"print"},{"value":"2629-3277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,7]]},"assertion":[{"value":"28 January 2023","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 February 2023","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests in this manuscript.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}