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The aim of this study was to update the methods of experimental intrasplenic cell transplantation using human amniotic epithelial cells (hAECs) which are promising cells in the treatment of liver diseases. BALB\/c mice were administered intrasplenically with 0.5, 1, and 2 million hAECs by direct bolus injection (400\u00a0\u00b5l\/min) and via a subcutaneous splenic port by fast (20\u00a0\u03bcl\/min) and slow (10\u00a0\u03bcl\/min) infusion. The port was prepared by translocating the spleen to the skin pocket. The spleen, liver, and lungs were collected at 3\u00a0h, 6\u00a0h, and 24\u00a0h after the administration of cells. The distribution of hAECs, histopathological changes in the organs, complete blood count, and biochemical markers of liver damage were assessed. It has been shown that the method of intrasplenic cell administration affects the degree of liver damage. The largest number of mice showing significant liver damage was observed after direct administration and the lowest after slow administration through a port. Liver damage increased with the number of administered cells, which, paradoxically, resulted in increased liver colonization efficiency. It was concluded that the administration of 1\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> hAECs by slow infusion via a subcutaneous splenic port reduces the incidence of complications at the expense of a slight decrease in the effectiveness of implantation of the transplanted cells in the liver.<\/jats:p>","DOI":"10.1007\/s12015-024-10735-1","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T02:01:44Z","timestamp":1716256904000},"page":"1599-1617","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimization of methods for intrasplenic administration of human amniotic epithelial cells in order to perform safe and effective cell-based therapy for liver diseases"],"prefix":"10.1007","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0376-5629","authenticated-orcid":false,"given":"Piotr","family":"Czekaj","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4747-4962","authenticated-orcid":false,"given":"Mateusz","family":"Kr\u00f3l","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7485-0823","authenticated-orcid":false,"given":"Emanuel","family":"Kolanko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7598-1817","authenticated-orcid":false,"given":"Patrycja","family":"Wieczorek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0876-6003","authenticated-orcid":false,"given":"Edyta","family":"Bogunia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1923-263X","authenticated-orcid":false,"given":"Mateusz","family":"Hermyt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-7547","authenticated-orcid":false,"given":"Aniela","family":"Grajoszek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6067-1565","authenticated-orcid":false,"given":"Agnieszka","family":"Prusek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,21]]},"reference":[{"issue":"1","key":"10735_CR1","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1089\/scd.2013.0391","volume":"22","author":"SC Strom","year":"2013","unstructured":"Strom, S. 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European Journal of Gastroenterology and Hepatology,23(10), 936\u2013941. https:\/\/doi.org\/10.1097\/MEG.0b013e3283488b00","journal-title":"European Journal of Gastroenterology and Hepatology"}],"container-title":["Stem Cell Reviews and Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12015-024-10735-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12015-024-10735-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12015-024-10735-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T10:26:14Z","timestamp":1723458374000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12015-024-10735-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,21]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10735"],"URL":"https:\/\/doi.org\/10.1007\/s12015-024-10735-1","relation":{},"ISSN":["2629-3269","2629-3277"],"issn-type":[{"value":"2629-3269","type":"print"},{"value":"2629-3277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,21]]},"assertion":[{"value":"11 May 2024","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2024","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflicts of Interest"}},{"value":"Informed consent was obtained from all patients involved in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed Consent"}},{"value":"The study was conducted in accordance with the Declaration of Helsinki, and approved by the Bioethics Committee of the Medical University of Silesia (SUM) in Katowice (decision no. KNW\/0022\/KB\/29c\/19) for studies involving human cells. The animal study protocol was approved by the Institutional Ethical Committee for Animal Experiments of Medical University of Silesia, Katowice, Poland (decision no. 35\/2021 and 69\/2019).","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Institutional Review Board"}}]}}