{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T23:08:08Z","timestamp":1778627288204,"version":"3.51.4"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biotechnol"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1007\/s12033-019-00201-6","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T09:02:44Z","timestamp":1564131764000},"page":"763-773","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Novel Expression Vectors Based on the pIGDM1 Plasmid"],"prefix":"10.1007","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0954-291X","authenticated-orcid":false,"given":"Diana","family":"Mikiewicz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Plucienniczak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bierczynska-Krzysik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Skowronek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grzegorz","family":"Wegrzyn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,7,26]]},"reference":[{"key":"201_CR1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/0076-6879(90)85005-9","volume":"185","author":"P Balbas","year":"1990","unstructured":"Balbas, P., & Bolivar, F. (1990). Design and construction of expression plasmid vectors in Escherichia coli. Methods in Enzymology,\n                           185, 14\u201337. \n                    https:\/\/doi.org\/10.1016\/0076-6879(90)85005-9\n                    \n                  .","journal-title":"Methods in Enzymology"},{"key":"201_CR2","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/978-3-319-27216-0_2","volume":"896","author":"FJ Fern\u00e1ndez","year":"2016","unstructured":"Fern\u00e1ndez, F. J., & Vega, M. C. (2016). Choose a suitable expression host: A survey of available protein production platforms. Advances in Experimental Medicine and Biology,\n                           896, 15\u201324. \n                    https:\/\/doi.org\/10.1007\/978-3-319-27216-0_2\n                    \n                  .","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"201_CR3","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-1-4939-6887-9_1","volume":"1586","author":"O Gileadi","year":"2017","unstructured":"Gileadi, O. (2017). Recombinant protein expression in E. coli: A historical perspective. Methods in Molecular Biology,\n                           1586, 3\u201310. \n                    https:\/\/doi.org\/10.1007\/978-1-4939-6887-9_1\n                    \n                  .","journal-title":"Methods in Molecular Biology"},{"key":"201_CR4","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1007\/s00253-016-8045-z","volume":"101","author":"L Marschall","year":"2017","unstructured":"Marschall, L., Sagmeister, P., & Herwig, C. (2017). Tunable recombinant protein expression in E. coli: Promoter systems and genetic constraints. Applied Microbiology and Biotechnology,\n                           101, 501\u2013512. \n                    https:\/\/doi.org\/10.1007\/s00253-016-8045-z\n                    \n                  .","journal-title":"Applied Microbiology and Biotechnology"},{"issue":"3","key":"201_CR5","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1128\/MMBR.60.3.512-538.1996","volume":"60","author":"S Makrides","year":"1996","unstructured":"Makrides, S. (1996). Strategies for achieving high-level expression of genes in Escherichia coli. Microbiological Reviews,\n                           60(3), 512\u2013538.","journal-title":"Microbiological Reviews"},{"issue":"7","key":"201_CR6","doi-asserted-by":"publisher","first-page":"346","DOI":"10.14737\/journal.aavs\/2016\/4.7.346.356","volume":"4","author":"AR Gomes","year":"2016","unstructured":"Gomes, A. R., Byregowda, S. M., Veeregowda, B. M., & Balamurugan, V. (2016). An overview of heterologous expression host systems for the production of recombinant proteins. Advances in Animal Veterinary Sciences,\n                           4(7), 346\u2013356. \n                    https:\/\/doi.org\/10.14737\/journal.aavs\/2016\/4.7.346.356\n                    \n                  .","journal-title":"Advances in Animal Veterinary Sciences"},{"key":"201_CR7","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1016\/j.ijbiomac.2017.08.080","volume":"106","author":"J Kaur","year":"2018","unstructured":"Kaur, J., Kumar, A., & Kaur, J. (2018). Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements. International Journal of Biological Macromolecules,\n                           106, 803\u2013822. \n                    https:\/\/doi.org\/10.1016\/j.ijbiomac.2017.08.080\n                    \n                  .","journal-title":"International Journal of Biological Macromolecules"},{"key":"201_CR8","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.pep.2017.11.005","volume":"144","author":"I Ahmad","year":"2018","unstructured":"Ahmad, I., Nawaz, N., Darwesh, N., Rahman, S., Mustafa, M., Khan, S., et al. (2018). Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli. Protein Expression and Purification,\n                           144, 12\u201318. \n                    https:\/\/doi.org\/10.1016\/j.pep.2017.11.005\n                    \n                  .","journal-title":"Protein Expression and Purification"},{"key":"201_CR9","first-page":"24","volume-title":"The Merck manual of diagnosis and therapy","year":"1992","unstructured":"Berkow, R. (Ed.). (1992). The Merck manual of diagnosis and therapy (16th ed ed., pp. 24\u201330). Rahway: Merck Research Laboratories.","edition":"16"},{"key":"201_CR10","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/b12440","volume":"86","author":"K Friehs","year":"2004","unstructured":"Friehs, K. (2004). Plasmid copy number and plasmid stability. Advances in Biochemical Engineering\/Biotechnology,\n                           86, 47\u201382.","journal-title":"Advances in Biochemical Engineering\/Biotechnology"},{"issue":"2","key":"201_CR11","doi-asserted-by":"publisher","first-page":"322","DOI":"10.2144\/01312st03","volume":"31","author":"N Kholod","year":"2001","unstructured":"Kholod, N., & Mustelin, T. (2001). Novel vectors for co-expression of two proteins in E. coli. Biotechniques,\n                           31(2), 322\u2013328.","journal-title":"Biotechniques"},{"key":"201_CR12","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.1107\/S0907444906031003","volume":"62","author":"Ch Romier","year":"2006","unstructured":"Romier, Ch., Jelloul, M. B., Albeck, Sh, Buchwald, G., Busso, D., Celie, P., et al. (2006). Co-expression of protein complexes in prokaryotic and eukaryotic hosts: Experimental procedures, database tracking and case studies. Acta Crystallographica. Section D,\n                           62, 1232\u20131242. \n                    https:\/\/doi.org\/10.1107\/S0907444906031003\n                    \n                  .","journal-title":"Acta Crystallographica. Section D"},{"key":"201_CR13","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.pep.2009.09.002","volume":"69","author":"J Zeng","year":"2010","unstructured":"Zeng, J., Zhang, L., Li, Y., Wang, Y., Wang, M., Duan, X., et al. (2010). Over-producing soluble protein complex and validating protein\u2013protein interaction through a new bacterial co-expression system. Protein Expression and Purification,\n                           69, 47\u201353. \n                    https:\/\/doi.org\/10.1016\/j.pep.2009.09.002\n                    \n                  .","journal-title":"Protein Expression and Purification"},{"key":"201_CR14","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1006\/plas.1997.1312","volume":"38","author":"D Mikiewicz","year":"1997","unstructured":"Mikiewicz, D., Wrobel, B., Wegrzyn, G., & Plucienniczak, A. (1997). Isolation and characterization of a ColE1-like plasmid from Enterobacter agglomerans with a novel variant of rom gene. Plasmid,\n                           38, 210\u2013219.","journal-title":"Plasmid"},{"issue":"3","key":"201_CR15","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.plasmid.2011.12.011","volume":"67","author":"P Zaleski","year":"2012","unstructured":"Zaleski, P., Wawrzyniak, P., Sobolewska, A., Mikiewicz, D., Wojtowicz-Krawiec, A., Chojnacka-Puchta, L., et al. (2012). New cloning and expression vector derived from Escherichia coli plasmid pIGWZ12; A potential vector for a two-plasmid expression system. Plasmid,\n                           67(3), 264\u2013271. \n                    https:\/\/doi.org\/10.1016\/j.plasmid.2011.12.011\n                    \n                  .","journal-title":"Plasmid"},{"issue":"3\u20134","key":"201_CR16","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/0378-1119(90)90328-O","volume":"9","author":"X Soberon","year":"1980","unstructured":"Soberon, X., Covarrubias, L., & Bolivar, F. (1980). Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene,\n                           9(3\u20134), 287\u2013305.","journal-title":"Gene"},{"key":"201_CR17","doi-asserted-by":"publisher","first-page":"7583","DOI":"10.1093\/nar\/16.15.7583","volume":"16","author":"JM Short","year":"1998","unstructured":"Short, J. M., Fernandez, J. M., Sorge, J. A., & Huse, W. D. (1998). Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Research,\n                           16, 7583\u20137600. \n                    https:\/\/doi.org\/10.1093\/nar\/16.15.7583\n                    \n                  .","journal-title":"Nucleic Acids Research"},{"issue":"22","key":"201_CR18","doi-asserted-by":"publisher","first-page":"9494","DOI":"10.1093\/nar\/17.22.9494","volume":"17","author":"MA Alting-Mees","year":"1989","unstructured":"Alting-Mees, M. A., & Short, J. M. (1989). pBluescript II: Gene mapping vectors. Nucleic Acids Research,\n                           17(22), 9494. \n                    https:\/\/doi.org\/10.1093\/nar\/17.22.9494\n                    \n                  .","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"201_CR19","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/0378-1119(85)90120-9","volume":"33","author":"C Yanisch-Perron","year":"1985","unstructured":"Yanisch-Perron, C., Vieira, J., & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene,\n                           33(1), 103\u2013119. \n                    https:\/\/doi.org\/10.1016\/0378-1119(85)90120-9\n                    \n                  .","journal-title":"Gene"},{"key":"201_CR20","unstructured":"Plucienniczak A, Smorawinska M, Wolinowska R, Mikiewicz-Sygula D, Jagiello A, Kaczanowski R, et al. (2011) New plasmids, their derivatives and fragments, their methods of manufacture and application. US Patent No. US7868148B2"},{"key":"201_CR21","volume-title":"Molecular cloning: A laboratory manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: A laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press."},{"key":"201_CR22","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/1475-2859-11-109","volume":"11","author":"M Kesik-Brodacka","year":"2012","unstructured":"Kesik-Brodacka, M., Romanik, A., Mikiewicz-Sygula, D., Plucienniczak, G., & Plucienniczak, A. (2012). A novel system for stable, high-level expression from the T7 promoter. Microbial Cell Factories,\n                           11, 109. \n                    https:\/\/doi.org\/10.1186\/1475-2859-11-109\n                    \n                  .","journal-title":"Microbial Cell Factories"},{"key":"201_CR23","unstructured":"Plucienniczak A, Sokolowska I, Marciniak-Rusek A, Lukasiewicz N, Chojnacka L, Smorawinska M, et al. (2005) Method for production of recombinant growth hormone in form of hybrid protein. Patent No. WO2005066208."},{"key":"201_CR24","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/s12934-014-0113-4","volume":"13","author":"A Wojtowicz-Krawiec","year":"2014","unstructured":"Wojtowicz-Krawiec, A., Sokolowska, I., Smorawinska, M., Chojnacka-Puchta, L., Mikiewicz, D., Lukasiewicz, N., et al. (2014). Use of Ubp1 protease analog to produce recombinant human growth hormone in Escherichia coli. Microbial Cell Factories,\n                           13, 113. \n                    https:\/\/doi.org\/10.1186\/s12934-014-0113-4\n                    \n                  .","journal-title":"Microbial Cell Factories"},{"key":"201_CR25","doi-asserted-by":"publisher","first-page":"2641","DOI":"10.1101\/gad.8.21.2641","volume":"8","author":"GFT Chen","year":"1994","unstructured":"Chen, G. F. T., & Inouye, M. (1994). Role of the AGA\/AGG codons, the rarest codons in global gene expression in Escherichia coli. Genes and Development,\n                           8, 2641\u20132652.","journal-title":"Genes and Development"},{"key":"201_CR26","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.pep.2019.02.002","volume":"157","author":"M Zieli\u0144ski","year":"2019","unstructured":"Zieli\u0144ski, M., Romanik-Chru\u015bcielewska, A., Mikiewicz, D., \u0141ukasiewicz, N., Soko\u0142owska, I., Antosik, J., et al. (2019). Expression and purification of recombinant human insulin from E. coli 20 strain. Protein Expression and Purification,\n                           157, 63\u201369. \n                    https:\/\/doi.org\/10.1016\/j.pep.2019.02.002\n                    \n                  .","journal-title":"Protein Expression and Purification"}],"container-title":["Molecular Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12033-019-00201-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12033-019-00201-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12033-019-00201-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T23:35:49Z","timestamp":1595633749000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12033-019-00201-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,26]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["201"],"URL":"https:\/\/doi.org\/10.1007\/s12033-019-00201-6","relation":{},"ISSN":["1073-6085","1559-0305"],"issn-type":[{"value":"1073-6085","type":"print"},{"value":"1559-0305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,26]]},"assertion":[{"value":"26 July 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}