{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:14:02Z","timestamp":1791000842749,"version":"4.1.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"Natural Science Research Projects Fund in Colleges and Universities of Anhui Province","award":["KJ2019A0327"],"award-info":[{"award-number":["KJ2019A0327"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biotechnol"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1007\/s12033-021-00412-w","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T02:09:44Z","timestamp":1634177384000},"page":"330-337","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Study on the Effect and Mechanism of miR-185 on Lower Extremity Deep Venous Thrombosis"],"prefix":"10.1007","volume":"64","author":[{"given":"Ran","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haobo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaowen","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonglin","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1020-5037","authenticated-orcid":false,"given":"Yong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,14]]},"reference":[{"key":"412_CR1","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1161\/ATVBAHA.109.200766","volume":"30","author":"B Patterson","year":"2010","unstructured":"Patterson, B., Hinchliffe, R., Loftus, I., Thompson, M., & Holt, P. (2010). Indications for catheter-directed thrombolysis in the management of acute proximal deep venous thrombosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 30, 669\u2013674.","journal-title":"Arteriosclerosis, Thrombosis, and Vascular Biology"},{"key":"412_CR2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021004727","author":"L Avila","year":"2021","unstructured":"Avila, L., Amiri, N., De, R., Vincelli, J., Pullenayegum, E., Williams, S., & Brandao, L. (2021). Characteristics of upper and lower extremity deep vein thrombosis and predictors of post-thrombotic syndrome in children. Blood Advances. https:\/\/doi.org\/10.1182\/bloodadvances.2021004727","journal-title":"Blood Advances"},{"key":"412_CR3","doi-asserted-by":"publisher","DOI":"10.1007\/s00423-021-02315-5","author":"K Suzuki","year":"2021","unstructured":"Suzuki, K., Shibasaki, S., Nakauchi, M., Nakamura, K., Akimoto, S., Tanaka, T., Kikuchi, K., Inaba, K., Uyama, I., & Suda, K. (2021). Impact of routine preoperative sonographic screening with early intervention for deep venous thrombosis in lower extremities on preventing postoperative venous thromboembolism in patients with gastric cancer scheduled for minimally invasive surgery. Langenbeck\u2019s Archives of Surgery. https:\/\/doi.org\/10.1007\/s00423-021-02315-5","journal-title":"Langenbeck\u2019s Archives of Surgery"},{"key":"412_CR4","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1016\/j.jemermed.2016.11.020","volume":"52","author":"B Long","year":"2017","unstructured":"Long, B., & Koyfman, A. (2017). Best clinical practice: Controversies in outpatient management of acute pulmonary embolism. The Journal of Emergency Medicine, 52, 668\u2013679.","journal-title":"The Journal of Emergency Medicine"},{"key":"412_CR5","doi-asserted-by":"publisher","first-page":"1499","DOI":"10.1182\/blood-2017-03-743211","volume":"130","author":"Y Hisada","year":"2017","unstructured":"Hisada, Y., & Mackman, N. (2017). Cancer-associated pathways and biomarkers of venous thrombosis. Blood, 130, 1499\u20131506.","journal-title":"Blood"},{"key":"412_CR6","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1002\/iub.2168","volume":"72","author":"S Sun","year":"2020","unstructured":"Sun, S., Chai, S., Zhang, F., & Lu, L. (2020). Overexpressed microRNA-103a-3p inhibits acute lower-extremity deep venous thrombosis via inhibition of CXCL12. IUBMB Life, 72, 492\u2013504.","journal-title":"IUBMB Life"},{"key":"412_CR7","first-page":"493","volume":"16","author":"Y Sun","year":"2008","unstructured":"Sun, Y., Wu, R., Liu, W., & Huang, J. (2008). Analysis of the dynamic change of TF, TFPI and IL-1beta in plasma of patients with acute leukemia. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 16, 493\u2013496.","journal-title":"Zhongguo Shi Yan Xue Ye Xue Za Zhi"},{"key":"412_CR8","doi-asserted-by":"publisher","first-page":"S1206","DOI":"10.1245\/s10434-015-4592-2","volume":"22","author":"D Unruh","year":"2015","unstructured":"Unruh, D., Sagin, F., Adam, M., Van Dreden, P., Woodhams, B., Hart, K., Lindsell, C., Ahmad, S., & Bogdanov, V. (2015). Levels of alternatively spliced tissue factor in the plasma of patients with pancreatic cancer may help predict aggressive tumor phenotype. Annals of Surgical Oncology, 22, S1206-1211.","journal-title":"Annals of Surgical Oncology"},{"key":"412_CR9","first-page":"1471","volume":"26","author":"H Liu","year":"2018","unstructured":"Liu, H., Sun, N., Huang, Y., Su, X., Deng, D., & Cheng, P. (2018). Clinical significance of detecting tissue factor and tissue factor pathway inhibitor in thalassemia patients. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 26, 1471\u20131476.","journal-title":"Zhongguo Shi Yan Xue Ye Xue Za Zhi"},{"key":"412_CR10","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.blre.2013.03.001","volume":"27","author":"K Winckers","year":"2013","unstructured":"Winckers, K., ten Cate, H., & Hackeng, T. (2013). The role of tissue factor pathway inhibitor in atherosclerosis and arterial thrombosis. Blood Reviews, 27, 119\u2013132.","journal-title":"Blood Reviews"},{"key":"412_CR11","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.molcel.2017.01.023","volume":"65","author":"C Ziegenhain","year":"2017","unstructured":"Ziegenhain, C., Vieth, B., Parekh, S., Reinius, B., Guillaumet-Adkins, A., Smets, M., Leonhardt, H., Heyn, H., Hellmann, I., & Enard, W. (2017). Comparative analysis of single-cell RNA sequencing methods. Molecular Cell, 65, 631-643.e634.","journal-title":"Molecular Cell"},{"key":"412_CR12","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.omtn.2021.05.023","volume":"25","author":"M Hosen","year":"2021","unstructured":"Hosen, M., Li, Q., Liu, Y., Zietzer, A., Maus, K., Goody, P., Uchida, S., Latz, E., Werner, N., Nickenig, G., & Jansen, F. (2021). PUNISHERCAD increases the long noncoding RNA in small extracellular vesicles and regulates endothelial cell function via vesicular shuttling. Molecular Therapy. Nucleic Acids, 25, 388\u2013405.","journal-title":"Molecular Therapy. Nucleic Acids"},{"key":"412_CR13","doi-asserted-by":"publisher","first-page":"e0256440","DOI":"10.1371\/journal.pone.0256440","volume":"16","author":"H Abe","year":"2021","unstructured":"Abe, H., Kunita, A., Otake, Y., Kanda, T., Kaneda, A., Ushiku, T., & Fukayama, M. (2021). Virus\u2013host interactions in carcinogenesis of Epstein-Barr virus-associated gastric carcinoma: Potential roles of lost ARID1A expression in its early stage. PLoS ONE, 16, e0256440.","journal-title":"PLoS ONE"},{"key":"412_CR14","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1038\/cmi.2014.67","volume":"12","author":"S Saravanan","year":"2015","unstructured":"Saravanan, S., Thirugnanasambantham, K., Hanieh, H., Karikalan, K., Sekar, D., Rajagopalan, R., & Hairul Islam, V. (2015). miRNA-24 and miRNA-466i-5p controls inflammation in rat hepatocytes. Cellular & Molecular Immunology, 12, 113\u2013115.","journal-title":"Cellular & Molecular Immunology"},{"key":"412_CR15","first-page":"131","volume":"17","author":"Z Wen","year":"2018","unstructured":"Wen, Z., Zhang, J., Tang, P., Tu, N., Wang, K., & Wu, G. (2018). Overexpression of miR-185 inhibits autophagy and apoptosis of dopaminergic neurons by regulating the AMPK\/mTOR signaling pathway in Parkinson\u2019s disease. Molecular Medicine Reports, 17, 131\u2013137.","journal-title":"Molecular Medicine Reports"},{"key":"412_CR16","doi-asserted-by":"publisher","first-page":"2787","DOI":"10.1002\/jcb.26447","volume":"119","author":"Q Zhang","year":"2018","unstructured":"Zhang, Q., Chen, B., Liu, P., & Yang, J. (2018). XIST promotes gastric cancer (GC) progression through TGF-\u03b21 via targeting miR-185. Journal of Cellular Biochemistry, 119, 2787\u20132796.","journal-title":"Journal of Cellular Biochemistry"},{"key":"412_CR17","doi-asserted-by":"publisher","first-page":"2442","DOI":"10.3892\/ol.2017.5699","volume":"13","author":"J He","year":"2017","unstructured":"He, J., Tian, N., Yang, Y., Jin, L., Feng, X., Hua, J., Lin, S., Wang, B., Li, H., & Wang, J. (2017). miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma. Oncology Letters, 13, 2442\u20132448.","journal-title":"Oncology Letters"},{"key":"412_CR18","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/j.atherosclerosis.2015.10.026","volume":"243","author":"H Jiang","year":"2015","unstructured":"Jiang, H., Zhang, J., Du, Y., Jia, X., Yang, F., Si, S., Wang, L., & Hong, B. (2015). microRNA-185 modulates low density lipoprotein receptor expression as a key posttranscriptional regulator. Atherosclerosis, 243, 523\u2013532.","journal-title":"Atherosclerosis"},{"key":"412_CR19","first-page":"674","volume":"38","author":"X Liu","year":"2018","unstructured":"Liu, X., Wang, D., Yang, X., & Lei, L. (2018). Huayu Tongmai Granules protects against vascular endothelial dysfunction via up-regulating miR-185 and down-regulating RAGE. Bioscience Reports, 38, 674.","journal-title":"Bioscience Reports"},{"key":"412_CR20","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.18632\/aging.101843","volume":"11","author":"W Ni","year":"2019","unstructured":"Ni, W., Xia, Y., Bi, Y., Wen, F., Hu, D., & Luo, L. (2019). FoxD2-AS1 promotes glioma progression by regulating miR-185-5P\/HMGA2 axis and PI3K\/AKT signaling pathway. Aging, 11, 1427\u20131439.","journal-title":"Aging"},{"key":"412_CR21","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1016\/j.cpcardiol.2006.08.002","volume":"31","author":"S Allaqaband","year":"2006","unstructured":"Allaqaband, S., Solis, J., Kazemi, S., & Bajwa, T. (2006). Endovascular treatment of peripheral vascular disease. Current Problems in Cardiology, 31, 711\u2013760.","journal-title":"Current Problems in Cardiology"},{"key":"412_CR22","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1055\/s-0035-1563376","volume":"77","author":"E Fink","year":"2015","unstructured":"Fink, E. (2015). Health behavior from the gender perspective\u2013The concept of \u201cdoing genders\u201d and the perspective of intersectionality as an explanation. Gesundheitswesen (Bundesverband der Arzte des Offentlichen Gesundheitsdienstes (Germany)), 77, 880.","journal-title":"Gesundheitswesen (Bundesverband der Arzte des Offentlichen Gesundheitsdienstes (Germany))"},{"key":"412_CR23","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1007\/s10549-014-3081-9","volume":"147","author":"M Pande","year":"2014","unstructured":"Pande, M., Bondy, M., Do, K., Sahin, A., Ying, J., Mills, G., Thompson, P., & Brewster, A. (2014). Association between germline single nucleotide polymorphisms in the PI3K-AKT-mTOR pathway, obesity, and breast cancer disease-free survival. Breast Cancer Research and Treatment, 147, 381\u2013387.","journal-title":"Breast Cancer Research and Treatment"},{"key":"412_CR24","first-page":"951724","volume":"12","author":"N Xu","year":"2012","unstructured":"Xu, N., Lao, Y., Zhang, Y., & Gillespie, D. (2012). Akt: A double-edged sword in cell proliferation and genome stability. Journal of Oncology, 12, 951724.","journal-title":"Journal of Oncology"},{"key":"412_CR25","doi-asserted-by":"publisher","first-page":"40","DOI":"10.3389\/fnmol.2011.00040","volume":"4","author":"O Kaidanovich-Beilin","year":"2011","unstructured":"Kaidanovich-Beilin, O., & Woodgett, J. (2011). GSK-3: Functional insights from cell biology and animal models. Frontiers in Molecular Neuroscience, 4, 40.","journal-title":"Frontiers in Molecular Neuroscience"},{"key":"412_CR26","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.pharmthera.2014.11.016","volume":"148","author":"E Beurel","year":"2015","unstructured":"Beurel, E., Grieco, S., & Jope, R. (2015). Glycogen synthase kinase-3 (GSK3): Regulation, actions, and diseases. Pharmacology & Therapeutics, 148, 114\u2013131.","journal-title":"Pharmacology & Therapeutics"},{"key":"412_CR27","doi-asserted-by":"publisher","first-page":"7085","DOI":"10.1007\/s13277-015-3420-4","volume":"36","author":"M Liang","year":"2015","unstructured":"Liang, M., Liu, J., Ji, H., Chen, M., Zhao, Y., Li, S., Zhang, X., & Li, J. (2015). A Aconitum coreanum polysaccharide fraction induces apoptosis of hepatocellular carcinoma (HCC) cells via pituitary tumor transforming gene 1 (PTTG1)-mediated suppression of the P13K\/Akt and activation of p38 MAPK signaling pathway and displays antitumor activity in vivo. Tumour Biology, 36, 7085\u20137091.","journal-title":"Tumour Biology"},{"key":"412_CR28","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1210\/mend.14.8.0500","volume":"14","author":"I Gonzalez-Robayna","year":"2000","unstructured":"Gonzalez-Robayna, I., Falender, A., Ochsner, S., Firestone, G., & Richards, J. (2000). Follicle-stimulating hormone (FSH) stimulates phosphorylation and activation of protein kinase B (PKB\/Akt) and serum and glucocorticoid-lnduced kinase (Sgk): evidence for A kinase-independent signaling by FSH in granulosa cells. Molecular Endocrinology (Baltimore, Md.), 14, 1283\u20131300.","journal-title":"Molecular Endocrinology (Baltimore, Md.)"},{"key":"412_CR29","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1002\/jcp.20696","volume":"208","author":"C Dogra","year":"2006","unstructured":"Dogra, C., Changotra, H., Wergedal, J., & Kumar, A. (2006). Regulation of phosphatidylinositol 3-kinase (PI3K)\/Akt and nuclear factor-kappa B signaling pathways in dystrophin-deficient skeletal muscle in response to mechanical stretch. Journal of Cellular Physiology, 208, 575\u2013585.","journal-title":"Journal of Cellular Physiology"},{"key":"412_CR30","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1016\/j.cell.2017.09.044","volume":"171","author":"S Wenzel","year":"2017","unstructured":"Wenzel, S., Tyurina, Y., Zhao, J., StCroix, C., Dar, H., Mao, G., Tyurin, V., Anthonymuthu, T., Kapralov, A., Amoscato, A., Mikulska-Ruminska, K., Shrivastava, I., Kenny, E., Yang, Q., Rosenbaum, J., Sparvero, L., Emlet, D., Wen, X., Minami, Y., \u2026 Kagan, V. (2017). PEBP1 wardens ferroptosis by enabling lipoxygenase generation of lipid death signals. Cell, 171, 628\u2013641.","journal-title":"Cell"},{"key":"412_CR31","doi-asserted-by":"publisher","first-page":"e0125028","DOI":"10.1371\/journal.pone.0125028","volume":"10","author":"J Atkinson","year":"2015","unstructured":"Atkinson, J., Rank, K., Zeng, Y., Capen, A., Yadav, V., Manro, J., Engler, T., & Chedid, M. (2015). Activating the Wnt\/\u03b2-catenin pathway for the treatment of melanoma-application of LY2090314, a novel selective inhibitor of glycogen synthase kinase-3. PLoS ONE, 10, e0125028.","journal-title":"PLoS ONE"}],"container-title":["Molecular Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12033-021-00412-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12033-021-00412-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12033-021-00412-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T09:18:08Z","timestamp":1644052688000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12033-021-00412-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,14]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["412"],"URL":"https:\/\/doi.org\/10.1007\/s12033-021-00412-w","relation":{},"ISSN":["1073-6085","1559-0305"],"issn-type":[{"value":"1073-6085","type":"print"},{"value":"1559-0305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,14]]},"assertion":[{"value":"7 March 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 September 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All experimental protocols were conducted in accordance with the principles and procedures outlined in the \u201cGuide for Care and Use of Laboratory animals\u201d and approved by the Ethics Committee of the First Affiliated Hospital of Bengbu Medical College.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}