{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T16:13:47Z","timestamp":1785946427065,"version":"3.56.0"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100004595","name":"Universiti Sains Malaysia","doi-asserted-by":"publisher","award":["R501-LR-RND003-0000000972-0000"],"award-info":[{"award-number":["R501-LR-RND003-0000000972-0000"]}],"id":[{"id":"10.13039\/501100004595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biotechnol"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1007\/s12033-025-01440-6","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T14:18:20Z","timestamp":1745504300000},"page":"1271-1279","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Role of Calcium Ions in Restoring Lipase Activity of Recombinant Human Lipoprotein Lipase Expressed in Bacteria"],"prefix":"10.1007","volume":"68","author":[{"given":"Chong Lee","family":"Ng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theam Soon","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5067-2073","authenticated-orcid":false,"given":"Yee Siew","family":"Choong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"issue":"7","key":"1440_CR1","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/j.tem.2016.04.013","volume":"27","author":"LG Fong","year":"2016","unstructured":"Fong, L. G., Young, S. G., Beigneux, A. P., Bensadoun, A., Oberer, M., Jiang, H., & Ploug, M. (2016). GPIHBP1 and plasma triglyceride metabolism. Trends in Endocrinology and Metabolism, 27(7), 455\u2013469.","journal-title":"Trends in Endocrinology and Metabolism"},{"issue":"13","key":"1440_CR2","doi-asserted-by":"publisher","first-page":"6319","DOI":"10.1073\/pnas.1900983116","volume":"116","author":"AP Beigneux","year":"2019","unstructured":"Beigneux, A. P., Allan, C. M., Sandoval, N. P., Cho, G. W., Heizer, P. J., Jung, R. S., & Stanhope, K. L. (2019). Lipoprotein lipase is active as a monomer. Proceedings of the National Academy of Sciences USA, 116(13), 6319\u20136328.","journal-title":"Proceedings of the National Academy of Sciences USA"},{"issue":"4","key":"1440_CR3","doi-asserted-by":"publisher","first-page":"575","DOI":"10.3803\/EnM.2022.402","volume":"37","author":"JH Moon","year":"2022","unstructured":"Moon, J. H., Kim, K., & Choi, S. H. (2022). Lipoprotein lipase: Is it a magic target for the treatment of hypertriglyceridemia. Endocrinology and Metabolism, 37(4), 575\u2013586.","journal-title":"Endocrinology and Metabolism"},{"key":"1440_CR4","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/j.atherosclerosis.2014.10.016","volume":"237","author":"Y Li","year":"2014","unstructured":"Li, Y., He, P.-P., Zhang, D.-W., Zheng, X.-L., Cayabyab, F. S., Yin, W.-D., & Tang, C.-K. (2014). Lipoprotein lipase: From gene to atherosclerosis. Atherosclerosis, 237, 597\u2013608.","journal-title":"Atherosclerosis"},{"issue":"8","key":"1440_CR5","doi-asserted-by":"publisher","first-page":"4266","DOI":"10.1016\/S0021-9258(19)39557-2","volume":"265","author":"IJ Goldberg","year":"1990","unstructured":"Goldberg, I. J., Scheraldi, C. A., Yacoub, L. K., Saxena, U., & Bisgaier, C. L. (1990). Lipoprotein apoC-II activation of lipoprotein lipase. Journal of Biological Chemistry, 265(8), 4266\u20134272.","journal-title":"Journal of Biological Chemistry"},{"issue":"2569","key":"1440_CR6","first-page":"1","volume":"14","author":"KH Gunn","year":"2023","unstructured":"Gunn, K. H., & Neher, S. B. (2023). Structure of dimeric lipoprotein lipase reveals a pore for hydrolysis of acyl chains. Nature Communications, 14(2569), 1\u201315.","journal-title":"Nature Communications"},{"issue":"1","key":"1440_CR7","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.tem.2020.11.005","volume":"32","author":"SA Wu","year":"2020","unstructured":"Wu, S. A., Kersten, S., & Qi, L. (2020). Lipoprotein lipase and its regulators: An unfolding story. Trends in Endocrinology and Metabolism, 32(1), 48\u201361.","journal-title":"Trends in Endocrinology and Metabolism"},{"key":"1440_CR8","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1007\/s00109-002-0384-9","volume":"80","author":"JR Mead","year":"2002","unstructured":"Mead, J. R., Irvine, S. A., & Ramji, D. P. (2002). Lipoprotein lipase: Structure, function, regulation, and role in disease. Journal of Molecular Medicine, 80, 753\u2013769.","journal-title":"Journal of Molecular Medicine"},{"key":"1440_CR9","first-page":"e53","volume":"20","author":"MV Eck","year":"2000","unstructured":"Eck, M. V., Zimmermann, R., Groot, P. H. E., Zechner, R., & Berkel, T. J. C. V. (2000). Role of macrophage-derived lipoprotein lipase in lipoprotein metaboolism and atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 20, e53\u2013e62.","journal-title":"Arteriosclerosis, Thrombosis, and Vascular Biology"},{"key":"1440_CR10","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1097\/00041433-200210000-00002","volume":"13","author":"K Preiss-Landl","year":"2002","unstructured":"Preiss-Landl, K., Zimmermann, R., Hammerle, G., & Zechner, R. (2002). Lipoprotein lipase: The regulation of tissue specific expression and its role in lipid and energy metabolism. Current Opinion in Lipidology, 13, 471\u2013481.","journal-title":"Current Opinion in Lipidology"},{"key":"1440_CR11","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1159\/000509932","volume":"93","author":"H Nordman","year":"2020","unstructured":"Nordman, H., J\u00e4\u00e4skel\u00e4inen, J., & Voutilainen, R. (2020). Birth size as a determinant of cardiometabolic risk factors in children. Hormone Research in P\u00e6diatrics, 93, 144\u2013153.","journal-title":"Hormone Research in P\u00e6diatrics"},{"key":"1440_CR12","first-page":"1","volume":"16","author":"R Shang","year":"2024","unstructured":"Shang, R., & Rodrigues, B. (2024). Lipoprotein lipase as a target for obesity\/diabetes related cardiovascular disease. Journal of Pharmacy & Pharmaceutical Sciences, 16, 1\u201312.","journal-title":"Journal of Pharmacy & Pharmaceutical Sciences"},{"issue":"e12095","key":"1440_CR13","first-page":"1","volume":"5","author":"S Mysling","year":"2016","unstructured":"Mysling, S., Kristensen, K. K., Larsson, M., Beigneux, A. P., G\u00e5rdsvoll, H., Fong, L. G., Bensadoun, A., J\u00f8rgensen, T. J., Young, S. G., & Ploug, M. (2016). The acidic domain of the endothelial membrane protein GPIHBP1 stabilizes lipoprotein lipase activity by preventing unfolding of its catalytic domain. eLife, 5(e12095), 1\u201324.","journal-title":"eLife"},{"key":"1440_CR14","first-page":"1","volume":"e20958","author":"S Mysling","year":"2016","unstructured":"Mysling, S., Kristensen, K. K., Larsson, M., Kovrov, O., Bensadoun, A., J\u00f8rgensen, T. J., Olivecrona, G., Young, S. G., & Ploug, M. (2016). The angiopoietin-like protein ANGPTL4 catalyzes unfolding of the hydrolase domain in lipoprotein lipase and the endothelial membrane protein GPIHBP1 counteracts this unfolding. eLife, e20958, 1\u201318.","journal-title":"eLife"},{"issue":"52","key":"1440_CR15","doi-asserted-by":"publisher","first-page":"42580","DOI":"10.1074\/jbc.M507252200","volume":"280","author":"L Zhang","year":"2005","unstructured":"Zhang, L., Lookene, A., Wu, G., & Olivecrona, G. (2005). Calcium triggers folding of lipoprotein lipase into active dimers. The Journal of Biological Chemistry, 280(52), 42580\u201342591.","journal-title":"The Journal of Biological Chemistry"},{"issue":"6","key":"1440_CR16","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1159\/000519410","volume":"14","author":"M Ohira","year":"2021","unstructured":"Ohira, M., Watanabe, Y., Yamaguchi, T., Onda, H., Yamaoka, S., Abe, K., Nakamura, S., Tanaka, S., Kawagoe, N., Nabekura, T., Oshiro, T., Nagayama, D., Tatsuno, I., & Saiki, A. (2021). Decreased triglyceride and increased serum lipoprotein lipase levels are correlated to increased high-density lipoprotein-cholesterol levels after laparoscopic sleeve gastrectomy. Obesity Facts, 14(6), 633\u2013640.","journal-title":"Obesity Facts"},{"issue":"100149","key":"1440_CR17","first-page":"1","volume":"62","author":"A-ML Winther","year":"2021","unstructured":"Winther, A.-M.L., Kristensen, K. K., Kumari, A., & Ploug, M. (2021). Expression and one-step purification of active LPL contemplated by biophysical considerations. Journal of Lipid Research, 62(100149), 1\u201313.","journal-title":"Journal of Lipid Research"},{"issue":"5","key":"1440_CR18","doi-asserted-by":"publisher","first-page":"1723","DOI":"10.1073\/pnas.1817984116","volume":"116","author":"G Birrane","year":"2019","unstructured":"Birrane, G., Beigneux, A. P., Dwyer, B., Strack-Logue, B., Kristensen, K. K., Francone, O. L., Fong, L. G., Mertens, H. D. T., Pan, C. Q., Ploug, M., Young, S. G., & Meiyappan, M. (2019). Structure of the lipoprotein lipase-GPIHBP1 complex that mediates plasma triglyceride hydrolysis. Proceedings of the National Academy of Sciences USA, 116(5), 1723\u20131732.","journal-title":"Proceedings of the National Academy of Sciences USA"},{"issue":"44","key":"1440_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1073\/pnas.2313825120","volume":"120","author":"W Song","year":"2023","unstructured":"Song, W., Beigneux, A. P., Weston, T. A., Chen, K., Yang, Y., Nguyen, L. P., Guagliardo, P., Jung, H., Tran, A. P., Tu, Y., Tran, C., Birrane, G., Miyashita, K., Nakajima, K., Murakami, M., Tontonoz, P., Jiang, H., Ploug, M., Fong, L. G., & Young, S. G. (2023). The lipoprotein lipase that is shuttled into capillaries by GPIHBP1 enters the glycocalyx where it mediates lipoprotein processing. PNAS, 120(44), 1\u201312.","journal-title":"PNAS"},{"issue":"24","key":"1440_CR20","doi-asserted-by":"publisher","first-page":"7791","DOI":"10.1016\/S0021-9258(19)57004-1","volume":"251","author":"P-H Iverius","year":"1976","unstructured":"Iverius, P.-H., & \u00d6stlund-Lindqvist, A.-M. (1976). Lipoprotein lipase from bovine milk. Journal of Biological Chemistry, 251(24), 7791\u20137795.","journal-title":"Journal of Biological Chemistry"},{"issue":"6","key":"1440_CR21","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1093\/jb\/mvy067","volume":"164","author":"N Kawaguchi","year":"2018","unstructured":"Kawaguchi, N., Date, K., Suzuki, Y., Tomita, C., Naradate, R., Higami, T., Nakamura, K., Aikawa, K., & Ogawa, H. (2018). A novel protocol for the preparation of active recombinant human pancreatic lipase from Escherichia coli. Journal of Biochemistry, 164(6), 407\u2013414.","journal-title":"Journal of Biochemistry"},{"issue":"8","key":"1440_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2460\/ajvr.22.03.0048","volume":"83","author":"SY Lim","year":"2022","unstructured":"Lim, S. Y., Steiner, J. M., & Cridge, H. (2022). Lipases: It\u2019s not just pancreatic lipase! American Journal of Veterinary Research, 83(8), 1\u20138.","journal-title":"American Journal of Veterinary Research"},{"key":"1440_CR23","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH image to imageJ: 25 years of image analysis. Nature Methods, 9, 671\u2013675.","journal-title":"Nature Methods"},{"issue":"3","key":"1440_CR24","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1016\/S0021-9258(19)68183-4","volume":"257","author":"K Shirai","year":"1982","unstructured":"Shirai, K., & Jackson, R. L. (1982). Lipoprotein lipase-catalyzed hydrolysis of p-nitrophenyl butyrate. The Journal of Biological Chemistry, 257(3), 1253\u20131258.","journal-title":"The Journal of Biological Chemistry"},{"key":"1440_CR25","doi-asserted-by":"publisher","first-page":"6872","DOI":"10.1021\/bi00269a038","volume":"21","author":"DM Quinn","year":"1982","unstructured":"Quinn, D. M., Shirai, K., Jackson, R. L., & Harmony, J. A. K. (1982). Lipoprotein lipase catalyzed hydrolysis of water-soluble p-nitrophenyl esters. Inhibition by apolipoprotein C-II. Biochemistry, 21, 6872\u20136879.","journal-title":"Biochemistry"},{"issue":"56","key":"1440_CR26","first-page":"1","volume":"11","author":"J Lobstein","year":"2012","unstructured":"Lobstein, J., Emrich, C. A., Jeans, C., Faulkner, M., Riggs, P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories, 11(56), 1\u201316.","journal-title":"Microbial Cell Factories"},{"issue":"2","key":"1440_CR27","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/0005-2760(86)90184-0","volume":"875","author":"E-Z Amri","year":"1986","unstructured":"Amri, E.-Z., Vannier, C., Etienne, J., & Ailhaud, G. (1986). Maturation and secretion of lipoprotein lipase in cultured adipose cells. II. Effects of tunicamycin on activation and secretion of the enzyme. Biochimica et Biophysica Acta, 875(2), 334\u2013343.","journal-title":"Biochimica et Biophysica Acta"}],"container-title":["Molecular Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12033-025-01440-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12033-025-01440-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12033-025-01440-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T06:26:31Z","timestamp":1774851991000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12033-025-01440-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["1440"],"URL":"https:\/\/doi.org\/10.1007\/s12033-025-01440-6","relation":{},"ISSN":["1073-6085","1559-0305"],"issn-type":[{"value":"1073-6085","type":"print"},{"value":"1559-0305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]},"assertion":[{"value":"27 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This work does not involve human or animal samples.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"All authors have approved for publication.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}}]}}