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We recently experienced an outbreak of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    culture lysis in our Melbourne-based structural biology labs that halted protein production despite our use of T1-resistant (\n                    <jats:italic>TonA<\/jats:italic>\n                    \/\n                    <jats:italic>FhuA<\/jats:italic>\n                    -disrupted) strains. Genetic analysis of the isolated phage yielded a 45,053\u00a0bp genome showing 80\u201390% identity with multiple Rtp-like siphophages, and transmission electron microscopy images were consistent with this classification. Further analysis revealed that our isolate was nearly identical to a highly virulent lytic coliphage MSK, recently isolated in Hangzhou, China, whose host receptor has not been determined. Sequence and structural modelling analysis of its putative receptor-binding protein suggested that its terminal receptor was likely to be LptD, an essential outer membrane protein involved in lipopolysaccharide transport. Based on a recent report of spontaneously arising mutations that blocked infection by other LptD-dependent bacteriophages, we designed a targeted genomic LptD loop deletion that successfully generated resistance to vB_EcoS_OzMSK in\n                    <jats:italic>E. coli<\/jats:italic>\n                    BL21(DE3) without apparent detriment to fitness. Here, we report a CRISPR-based, single-plasmid solution that will benefit other labs or facilities experiencing challenges due to LptD-dependent lytic phage outbreaks.\n                  <\/jats:p>","DOI":"10.1007\/s12033-025-01453-1","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T08:47:41Z","timestamp":1748940461000},"page":"1411-1423","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Resolution of a T1-Like Bacteriophage Outbreak by Receptor Engineering"],"prefix":"10.1007","volume":"68","author":[{"given":"Katrina A.","family":"Black","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie V.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolene R.","family":"Ramsey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack C.","family":"Tovey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L.","family":"Cameron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Alexandrovics","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alisa","family":"Glukhova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony T.","family":"Papenfuss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa J.","family":"Call","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryland","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5846-6469","authenticated-orcid":false,"given":"Matthew E.","family":"Call","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"1453_CR1","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1002\/bies.201400152","volume":"37","author":"EC Keen","year":"2015","unstructured":"Keen, E. 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