{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:27:00Z","timestamp":1793662020352,"version":"4.5.1"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100013039","name":"Yenepoya Research Centre","doi-asserted-by":"publisher","award":["YU\/Seed Grant\/092-2020"],"award-info":[{"award-number":["YU\/Seed Grant\/092-2020"]}],"id":[{"id":"10.13039\/501100013039","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Neurobiol"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1007\/s12035-022-02745-2","type":"journal-article","created":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T11:04:43Z","timestamp":1643195083000},"page":"2363-2377","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["In Vitro Oxygen Glucose Deprivation Model of Ischemic Stroke: A Proteomics-Driven Systems Biological Perspective"],"prefix":"10.1007","volume":"59","author":[{"given":"Manju","family":"Babu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9220-7812","authenticated-orcid":false,"given":"Arnab","family":"Datta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,26]]},"reference":[{"issue":"8","key":"2745_CR1","doi-asserted-by":"publisher","first-page":"3510","DOI":"10.1523\/jneurosci.13-08-03510.1993","volume":"13","author":"MP Goldberg","year":"1993","unstructured":"Goldberg MP, Choi DW (1993) Combined oxygen and glucose deprivation in cortical cell culture: calcium- dependent and calcium-independent mechanisms of neuronal injury. J Neurosci 13(8):3510\u20133524. https:\/\/doi.org\/10.1523\/jneurosci.13-08-03510.1993","journal-title":"J Neurosci"},{"key":"2745_CR2","doi-asserted-by":"publisher","first-page":"242","DOI":"10.12688\/f1000research.51752.2","volume":"10","author":"M Trotman-Lucas","year":"2021","unstructured":"Trotman-Lucas M, Gibson CL (2021) A review of experimental models of focal cerebral ischemia focusing on the middle cerebral artery occlusion model. F1000Res 10:242. https:\/\/doi.org\/10.12688\/f1000research.51752.2","journal-title":"F1000Res"},{"issue":"4926","key":"2745_CR3","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1126\/science.2675315","volume":"246","author":"JB Fenn","year":"1989","unstructured":"Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM (1989) Electrospray ionization for mass spectrometry of large biomolecules. Science 246(4926):64\u201371. https:\/\/doi.org\/10.1126\/science.2675315","journal-title":"Science"},{"issue":"8","key":"2745_CR4","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1002\/rcm.1290020802","volume":"2","author":"K Tanaka","year":"1988","unstructured":"Tanaka K, Waki H, Ido Y, Akita S, Yoshida Y, Yoshida T, Matsuo T (1988) Protein and polymer analyses up to m\/z 100 000 by laser ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2(8):151\u2013153. https:\/\/doi.org\/10.1002\/rcm.1290020802","journal-title":"Rapid Commun Mass Spectrom"},{"key":"2745_CR5","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1146\/annurev.genom.2.1.343","volume":"2","author":"T Ideker","year":"2001","unstructured":"Ideker T, Galitski T, Hood L (2001) A new approach to decoding life: systems biology. Annu Rev Genomics Hum Genet 2:343\u2013372. https:\/\/doi.org\/10.1146\/annurev.genom.2.1.343","journal-title":"Annu Rev Genomics Hum Genet"},{"issue":"3","key":"2745_CR6","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1002\/ana.20741","volume":"59","author":"VE O'Collins","year":"2006","unstructured":"O\u2019Collins VE, Macleod MR, Donnan GA, Horky LL, van der Worp BH, Howells DW (2006) 1,026 experimental treatments in acute stroke. Ann Neurol 59(3):467\u2013477. https:\/\/doi.org\/10.1002\/ana.20741","journal-title":"Ann Neurol"},{"issue":"10","key":"2745_CR7","doi-asserted-by":"publisher","first-page":"1753","DOI":"10.1517\/14656566.2010.493558","volume":"11","author":"PA Lapchak","year":"2010","unstructured":"Lapchak PA (2010) A critical assessment of edaravone acute ischemic stroke efficacy trials: is edaravone an effective neuroprotective therapy? Expert Opin Pharmacother 11(10):1753\u20131763. https:\/\/doi.org\/10.1517\/14656566.2010.493558","journal-title":"Expert Opin Pharmacother"},{"issue":"1","key":"2745_CR8","doi-asserted-by":"publisher","first-page":"134","DOI":"10.2174\/1570159x14666160620102055","volume":"15","author":"HS Chen","year":"2017","unstructured":"Chen HS, Qi SH, Shen JG (2017) One-compound-multi-target: combination prospect of natural compounds with thrombolytic therapy in acute ischemic stroke. Curr Neuropharmacol 15(1):134\u2013156. https:\/\/doi.org\/10.2174\/1570159x14666160620102055","journal-title":"Curr Neuropharmacol"},{"issue":"7","key":"2745_CR9","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1177\/0271678x20918031","volume":"40","author":"S Tonomura","year":"2020","unstructured":"Tonomura S, Ihara M, Friedland RP (2020) Microbiota in cerebrovascular disease: a key player and future therapeutic target. J Cereb Blood Flow Metab 40(7):1368\u20131380. https:\/\/doi.org\/10.1177\/0271678x20918031","journal-title":"J Cereb Blood Flow Metab"},{"issue":"11","key":"2745_CR10","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1038\/nn.2953","volume":"14","author":"C Iadecola","year":"2011","unstructured":"Iadecola C, Anrather J (2011) Stroke research at a crossroad: asking the brain for directions. Nat Neurosci 14(11):1363\u20131368. https:\/\/doi.org\/10.1038\/nn.2953","journal-title":"Nat Neurosci"},{"issue":"6","key":"2745_CR11","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1007\/s12975-019-00716-9","volume":"10","author":"H Li","year":"2019","unstructured":"Li H, You W, Li X, Shen H, Chen G (2019) Proteomic-based approaches for the study of ischemic stroke. Transl Stroke Res 10(6):601\u2013606. https:\/\/doi.org\/10.1007\/s12975-019-00716-9","journal-title":"Transl Stroke Res"},{"issue":"11","key":"2745_CR12","doi-asserted-by":"publisher","first-page":"5199","DOI":"10.1021\/pr200673y","volume":"10","author":"A Datta","year":"2011","unstructured":"Datta A, Jingru Q, Khor TH, Teo MT, Heese K, Sze SK (2011) Quantitative neuroproteomics of an in vivo rodent model of focal cerebral ischemia\/reperfusion injury reveals a temporal regulation of novel pathophysiological molecular markers. J Prot Res 10(11):5199\u20135213. https:\/\/doi.org\/10.1021\/pr200673y","journal-title":"J Prot Res"},{"issue":"10","key":"2745_CR13","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.tins.2013.07.001","volume":"36","author":"P Mergenthaler","year":"2013","unstructured":"Mergenthaler P, Lindauer U, Dienel GA, Meisel A (2013) Sugar for the brain: the role of glucose in physiological and pathological brain function. Trends Neurosci 36(10):587\u2013597. https:\/\/doi.org\/10.1016\/j.tins.2013.07.001","journal-title":"Trends Neurosci"},{"issue":"1","key":"2745_CR14","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/s12031-019-01279-y","volume":"68","author":"J Liu","year":"2019","unstructured":"Liu J, Chen M, Dong R, Sun C, Li S, Zhu S (2019) Ghrelin promotes cortical neurites growth in late stage after oxygen-glucose deprivation\/reperfusion injury. J Mol Neurosci 68(1):29\u201337. https:\/\/doi.org\/10.1007\/s12031-019-01279-y","journal-title":"J Mol Neurosci"},{"key":"2745_CR15","doi-asserted-by":"publisher","unstructured":"DeGregorio-Rocasolano N, Guirao V, Ponce J, Meli\u00e0-Sorolla M, Aliena-Valero A, Garc\u00eda-Serran A, Salom JB, D\u00e1valos A et al (2020) Comparative proteomics unveils LRRFIP1 as a new player in the DAPK1 interactome of neurons exposed to oxygen and glucose deprivation. Antioxidants (Basel, Switzerland) 9(12):1202. https:\/\/doi.org\/10.3390\/antiox9121202","DOI":"10.3390\/antiox9121202"},{"issue":"8","key":"2745_CR16","doi-asserted-by":"publisher","first-page":"2103","DOI":"10.1161\/strokeaha.116.012763","volume":"47","author":"JY Kim","year":"2016","unstructured":"Kim JY, Kim N, Zheng Z, Lee JE, Yenari MA (2016) 70-kDa Heat shock protein downregulates dynamin in experimental stroke: a new therapeutic target? Stroke 47(8):2103\u20132111. https:\/\/doi.org\/10.1161\/strokeaha.116.012763","journal-title":"Stroke"},{"issue":"2","key":"2745_CR17","doi-asserted-by":"publisher","first-page":"1108","DOI":"10.1021\/pr200834f","volume":"11","author":"W Yang","year":"2012","unstructured":"Yang W, Thompson JW, Wang Z, Wang L, Sheng H, Foster MW, Moseley MA, Paschen W (2012) Analysis of oxygen\/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics. J Proteome Res 11(2):1108\u20131117. https:\/\/doi.org\/10.1021\/pr200834f","journal-title":"J Proteome Res"},{"issue":"1","key":"2745_CR18","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1021\/pr900829h","volume":"9","author":"A Datta","year":"2010","unstructured":"Datta A, Park JE, Li X, Zhang H, Ho ZS, Heese K, Lim SK, Tam JP et al (2010) Phenotyping of an in vitro model of ischemic penumbra by iTRAQ-based shotgun quantitative proteomics. J Proteome Res 9(1):472\u2013484. https:\/\/doi.org\/10.1021\/pr900829h","journal-title":"J Proteome Res"},{"issue":"4","key":"2745_CR19","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1111\/jnc.13441","volume":"136","author":"SY Jeong","year":"2016","unstructured":"Jeong SY, Jeon R, Choi YK, Jung JE, Liang A, Xing C, Wang X, Lo EH et al (2016) Activation of microglial Toll-like receptor 3 promotes neuronal survival against cerebral ischemia. J Neurochem 136(4):851\u2013858. https:\/\/doi.org\/10.1111\/jnc.13441","journal-title":"J Neurochem"},{"key":"2745_CR20","doi-asserted-by":"publisher","first-page":"271","DOI":"10.3389\/fnmol.2017.00271","volume":"10","author":"B Zhang","year":"2017","unstructured":"Zhang B, Yang N, Mo ZM, Lin SP, Zhang F (2017) IL-17A Enhances microglial response to OGD by regulating p53 and PI3K\/Akt pathways with involvement of ROS\/HMGB1. Front Mol Neurosci 10:271. https:\/\/doi.org\/10.3389\/fnmol.2017.00271","journal-title":"Front Mol Neurosci"},{"issue":"11","key":"2745_CR21","doi-asserted-by":"publisher","first-page":"2673","DOI":"10.1089\/ars.2009.2730","volume":"11","author":"CM Snyder","year":"2009","unstructured":"Snyder CM, Chandel NS (2009) Mitochondrial regulation of cell survival and death during low-oxygen conditions. Antioxid Redox Signal 11(11):2673\u20132683. https:\/\/doi.org\/10.1089\/ars.2009.2730","journal-title":"Antioxid Redox Signal"},{"issue":"2","key":"2745_CR22","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1161\/strokeaha.115.011932","volume":"47","author":"PM Holloway","year":"2016","unstructured":"Holloway PM, Gavins FN (2016) Modeling ischemic stroke in vitro: status quo and future perspectives. Stroke 47(2):561\u2013569. https:\/\/doi.org\/10.1161\/strokeaha.115.011932","journal-title":"Stroke"},{"key":"2745_CR23","doi-asserted-by":"publisher","first-page":"272","DOI":"10.3389\/fncel.2020.00272","volume":"14","author":"H Li","year":"2020","unstructured":"Li H, Kittur FS, Hung C-Y, Li PA, Ge X, Sane DC, Xie J (2020) Quantitative proteomics reveals the beneficial effects of low glucose on neuronal cell survival in an in vitro ischemic penumbral model. Front Cell Neurosci 14:272. https:\/\/doi.org\/10.3389\/fncel.2020.00272","journal-title":"Front Cell Neurosci"},{"key":"2745_CR24","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s12975-020-00814-z","volume":"12","author":"X Zheng","year":"2021","unstructured":"Zheng X, Zhang L, Kuang Y, Venkataramani V, Jin F, Hein K, Zafeiriou MP, Lenz C et al (2021) Extracellular vesicles derived from neural progenitor cells\u2013a preclinical evaluation for stroke treatment in mice. Transl Stroke Res 12:185\u2013203. https:\/\/doi.org\/10.1007\/s12975-020-00814-z","journal-title":"Transl Stroke Res"},{"key":"2745_CR25","doi-asserted-by":"publisher","first-page":"110151","DOI":"10.1016\/j.biopha.2020.110151","volume":"127","author":"J Zhao","year":"2020","unstructured":"Zhao J, Piao X, Wu Y, Liang S, Han F, Liang Q, Shao S, Zhao D (2020) Cepharanthine attenuates cerebral ischemia\/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12\/15-LOX signaling. Biomed Pharmacother 127:110151. https:\/\/doi.org\/10.1016\/j.biopha.2020.110151","journal-title":"Biomed Pharmacother"},{"key":"2745_CR26","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1007\/s12264-018-0283-x","volume":"34","author":"F-F Xu","year":"2018","unstructured":"Xu F-F, Zhang Z-B, Wang Y-Y, Wang T-H (2018) Brain-derived glia maturation factor \u03b2 participates in lung injury induced by acute cerebral ischemia by increasing ROS in endothelial cells. Neurosci Bull 34:1077\u20131090. https:\/\/doi.org\/10.1007\/s12264-018-0283-x","journal-title":"Neurosci Bull"},{"issue":"1","key":"2745_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1089\/ars.2013.5381","volume":"21","author":"RR Tao","year":"2014","unstructured":"Tao RR, Wang H, Hong LJ, Huang JY, Lu YM, Liao MH, Ye WF, Lu NN et al (2014) Nitrosative stress induces peroxiredoxin 1 ubiquitination during ischemic insult via E6AP activation in endothelial cells both in vitro and in vivo. Antioxid Redox Signal 21(1):1\u201316. https:\/\/doi.org\/10.1089\/ars.2013.5381","journal-title":"Antioxid Redox Signal"},{"issue":"1","key":"2745_CR28","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1021\/pr0603811","volume":"6","author":"AS Haqqani","year":"2007","unstructured":"Haqqani AS, Kelly J, Baumann E, Haseloff RF, Blasig IE, Stanimirovic DB (2007) Protein markers of ischemic insult in brain endothelial cells identified using 2D gel electrophoresis and ICAT-based quantitative proteomics. J Proteome Res 6(1):226\u2013239. https:\/\/doi.org\/10.1021\/pr0603811","journal-title":"J Proteome Res"},{"key":"2745_CR29","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jprot.2018.06.011","volume":"184","author":"P Comajoan","year":"2018","unstructured":"Comajoan P, Gubern C, Huguet G, Serena J, K\u00e1d\u00e1r E, Castellanos M (2018) Evaluation of common housekeeping proteins under ischemic conditions and\/or rt-PA treatment in bEnd. 3 cells. J Proteomics 184:10\u201315. https:\/\/doi.org\/10.1016\/j.jprot.2018.06.011","journal-title":"J Proteomics"},{"issue":"7","key":"2745_CR30","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1038\/nbt.1658","volume":"28","author":"P Mallick","year":"2010","unstructured":"Mallick P, Kuster B (2010) Proteomics: a pragmatic perspective. Nat Biotechnol 28(7):695\u2013709. https:\/\/doi.org\/10.1038\/nbt.1658","journal-title":"Nat Biotechnol"},{"key":"2745_CR31","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1038\/jcbfm.2012.204","volume":"33","author":"AG Herrmann","year":"2013","unstructured":"Herrmann AG, Deighton RF, Le Bihan T, McCulloch MC, Searcy JL, Kerr LE, McCulloch J (2013) Adaptive changes in the neuronal proteome: mitochondrial energy production, endoplasmic reticulum stress, and ribosomal dysfunction in the cellular response to metabolic stress. J Cereb Blood Flow Metab 33:673\u2013683. https:\/\/doi.org\/10.1038\/jcbfm.2012.204","journal-title":"J Cereb Blood Flow Metab"},{"key":"2745_CR32","doi-asserted-by":"publisher","first-page":"116527","DOI":"10.1016\/j.lfs.2019.06.002","volume":"231","author":"X Wang","year":"2019","unstructured":"Wang X, Wang J, Shi X, Pan C, Liu H, Dong Y, Dong R, Mang J et al (2019) Proteomic analyses identify a potential mechanism by which extracellular vesicles aggravate ischemic stroke. Life Sci 231:116527. https:\/\/doi.org\/10.1016\/j.lfs.2019.06.002","journal-title":"Life Sci"},{"key":"2745_CR33","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.jprot.2015.12.011","volume":"133","author":"V Llombart","year":"2016","unstructured":"Llombart V, Garc\u00eda-Berrocoso T, Bech-Serra JJ, Simats A, Bustamante A, Giralt D, Reverter-Branchat G, Canals F et al (2016) Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC). J Proteomics 133:100\u2013112. https:\/\/doi.org\/10.1016\/j.jprot.2015.12.011","journal-title":"J Proteomics"},{"issue":"2","key":"2745_CR34","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1016\/j.bbrc.2009.12.015","volume":"391","author":"H Yan","year":"2010","unstructured":"Yan H, Zhou W, Wei L, Zhong F, Yang Y (2010) Proteomic analysis of astrocytic secretion that regulates neurogenesis using quantitative amine-specific isobaric tagging. Biochem Biophys Res Commun 391(2):1187\u20131191. https:\/\/doi.org\/10.1016\/j.bbrc.2009.12.015","journal-title":"Biochem Biophys Res Commun"},{"key":"2745_CR35","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.aca.2017.01.059","volume":"964","author":"V Vidova","year":"2017","unstructured":"Vidova V, Spacil Z (2017) A review on mass spectrometry-based quantitative proteomics: Targeted and data independent acquisition. Anal Chim Acta 964:7\u201323. https:\/\/doi.org\/10.1016\/j.aca.2017.01.059","journal-title":"Anal Chim Acta"},{"issue":"1","key":"2745_CR36","doi-asserted-by":"publisher","first-page":"3183","DOI":"10.1038\/s41598-019-39438-1","volume":"9","author":"MCE Jochner","year":"2019","unstructured":"Jochner MCE, An J, L\u00e4ttig-T\u00fcnnemann G, Kirchner M, Dagane A, Dittmar G, Dirnagl U, Eickholt BJ et al (2019) Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress. Sci Rep 9(1):3183. https:\/\/doi.org\/10.1038\/s41598-019-39438-1","journal-title":"Sci Rep"},{"key":"2745_CR37","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.neuroscience.2020.07.044","volume":"443","author":"T Imai","year":"2020","unstructured":"Imai T, Matsubara H, Nakamura S, Hara H, Shimazawa M (2020) The mitochondria-targeted peptide, bendavia, attenuated ischemia\/reperfusion-induced stroke damage. Neuroscience 443:110\u2013119. https:\/\/doi.org\/10.1016\/j.neuroscience.2020.07.044","journal-title":"Neuroscience"},{"issue":"9","key":"2745_CR38","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1038\/s42255-019-0105-0","volume":"1","author":"T Ast","year":"2019","unstructured":"Ast T, Mootha VK (2019) Oxygen and mammalian cell culture: are we repeating the experiment of Dr. Ox? Nat Metab 1(9):858\u2013860. https:\/\/doi.org\/10.1038\/s42255-019-0105-0","journal-title":"Nat Metab"},{"issue":"1","key":"2745_CR39","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1152\/physrev.00041.2017","volume":"99","author":"TP Keeley","year":"2019","unstructured":"Keeley TP, Mann GE (2019) Defining physiological normoxia for improved translation of cell physiology to animal models and humans. Physiol Rev 99(1):161\u2013234. https:\/\/doi.org\/10.1152\/physrev.00041.2017","journal-title":"Physiol Rev"},{"issue":"5","key":"2745_CR40","doi-asserted-by":"publisher","first-page":"L922","DOI":"10.1152\/ajplung.00014.2002","volume":"283","author":"C Schroedl","year":"2002","unstructured":"Schroedl C, McClintock DS, Budinger GR, Chandel NS (2002) Hypoxic but not anoxic stabilization of HIF-1alpha requires mitochondrial reactive oxygen species. Am J Physiol Lung Cell Mol Physiol 283(5):L922-931. https:\/\/doi.org\/10.1152\/ajplung.00014.2002","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"issue":"45","key":"2745_CR41","doi-asserted-by":"publisher","first-page":"31153","DOI":"10.1074\/jbc.M805056200","volume":"283","author":"L Liu","year":"2008","unstructured":"Liu L, Wise DR, Diehl JA, Simon MC (2008) Hypoxic reactive oxygen species regulate the integrated stress response and cell survival. J Biol Chem 283(45):31153\u201331162. https:\/\/doi.org\/10.1074\/jbc.M805056200","journal-title":"J Biol Chem"},{"key":"2745_CR42","doi-asserted-by":"publisher","first-page":"35","DOI":"10.2147\/hp.S92198","volume":"3","author":"RH Wenger","year":"2015","unstructured":"Wenger RH, Kurtcuoglu V, Scholz CC, Marti HH, Hoogewijs D (2015) Frequently asked questions in hypoxia research. Hypoxia (Auckl) 3:35\u201343. https:\/\/doi.org\/10.2147\/hp.S92198","journal-title":"Hypoxia (Auckl)"},{"issue":"2","key":"2745_CR43","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1016\/j.jneumeth.2007.08.028","volume":"167","author":"AM Kleman","year":"2008","unstructured":"Kleman AM, Yuan JY, Aja S, Ronnett GV, Landree LE (2008) Physiological glucose is critical for optimized neuronal viability and AMPK responsiveness in vitro. J Neurosci Methods 167(2):292\u2013301. https:\/\/doi.org\/10.1016\/j.jneumeth.2007.08.028","journal-title":"J Neurosci Methods"},{"key":"2745_CR44","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1016\/j.jprot.2013.08.017","volume":"91","author":"A Datta","year":"2013","unstructured":"Datta A, Akatsu H, Heese K, Sze SK (2013) Quantitative clinical proteomic study of autopsied human infarcted brain specimens to elucidate the deregulated pathways in ischemic stroke pathology. J Proteomics 91:556\u2013568. https:\/\/doi.org\/10.1016\/j.jprot.2013.08.017","journal-title":"J Proteomics"},{"issue":"4","key":"2745_CR45","doi-asserted-by":"publisher","first-page":"e94663","DOI":"10.1371\/journal.pone.0094663","volume":"9","author":"A Datta","year":"2014","unstructured":"Datta A, Chen CP, Sze SK (2014) Discovery of prognostic biomarker candidates of lacunar infarction by quantitative proteomics of microvesicles enriched plasma. PLoS ONE 9(4):e94663. https:\/\/doi.org\/10.1371\/journal.pone.0094663","journal-title":"PLoS ONE"},{"issue":"11","key":"2745_CR46","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1097\/NEN.0b013e3181f8c539","volume":"69","author":"E Cuadrado","year":"2010","unstructured":"Cuadrado E, Rosell A, Colom\u00e9 N, Hern\u00e1ndez-Guillamon M, Garc\u00eda-Berrocoso T, Ribo M, Alcazar A, Ortega-Aznar A et al (2010) The proteome of human brain after ischemic stroke. J Neuropathol Exp Neurol 69(11):1105\u20131115. https:\/\/doi.org\/10.1097\/NEN.0b013e3181f8c539","journal-title":"J Neuropathol Exp Neurol"},{"issue":"1","key":"2745_CR47","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1074\/mcp.RA117.000419","volume":"17","author":"T Garc\u00eda-Berrocoso","year":"2018","unstructured":"Garc\u00eda-Berrocoso T, Llombart V, Col\u00e0s-Camp\u00e0s L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A et al (2018) Single cell immuno-laser microdissection coupled to label-free proteomics to reveal the proteotypes of human brain cells after ischemia. Mol Cell Proteomics 17(1):175\u2013189. https:\/\/doi.org\/10.1074\/mcp.RA117.000419","journal-title":"Mol Cell Proteomics"},{"key":"2745_CR48","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.jprot.2013.09.005","volume":"94","author":"T Garc\u00eda-Berrocoso","year":"2013","unstructured":"Garc\u00eda-Berrocoso T, Penalba A, Boada C, Giralt D, Cuadrado E, Colom\u00e9 N, Dayon L, Canals F et al (2013) From brain to blood: new biomarkers for ischemic stroke prognosis. J Proteomics 94:138\u2013148. https:\/\/doi.org\/10.1016\/j.jprot.2013.09.005","journal-title":"J Proteomics"},{"issue":"8","key":"2745_CR49","doi-asserted-by":"publisher","first-page":"2242","DOI":"10.1002\/pmic.200300809","volume":"4","author":"L Allard","year":"2004","unstructured":"Allard L, Lescuyer P, Burgess J, Leung KY, Ward M, Walter N, Burkhard PR, Corthals G et al (2004) ApoC-I and ApoC-III as potential plasmatic markers to distinguish between ischemic and hemorrhagic stroke. Proteomics 4(8):2242\u20132251. https:\/\/doi.org\/10.1002\/pmic.200300809","journal-title":"Proteomics"},{"issue":"6","key":"2745_CR50","doi-asserted-by":"publisher","first-page":"2466","DOI":"10.1021\/pr501324n","volume":"14","author":"R Sharma","year":"2015","unstructured":"Sharma R, Gowda H, Chavan S, Advani J, Kelkar D, Kumar GS, Bhattacharjee M, Chaerkady R et al (2015) Proteomic signature of endothelial dysfunction identified in the serum of acute ischemic stroke patients by the iTRAQ-based LC-MS approach. J Proteome Res 14(6):2466\u20132479. https:\/\/doi.org\/10.1021\/pr501324n","journal-title":"J Proteome Res"},{"issue":"5","key":"2745_CR51","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1007\/s12975-018-0675-2","volume":"10","author":"H Song","year":"2019","unstructured":"Song H, Zhou H, Qu Z, Hou J, Chen W, Cai W, Cheng Q, Chuang DY et al (2019) From Analysis of ischemic mouse brain proteome to identification of human serum clusterin as a potential biomarker for severity of acute ischemic stroke. Transl Stroke Res 10(5):546\u2013556. https:\/\/doi.org\/10.1007\/s12975-018-0675-2","journal-title":"Transl Stroke Res"},{"issue":"8","key":"2745_CR52","doi-asserted-by":"publisher","first-page":"2234","DOI":"10.1002\/pmic.200300822","volume":"4","author":"P Lescuyer","year":"2004","unstructured":"Lescuyer P, Allard L, Zimmermann-Ivol CG, Burgess JA, Hughes-Frutiger S, Burkhard PR, Sanchez JC, Hochstrasser DF (2004) Identification of post-mortem cerebrospinal fluid proteins as potential biomarkers of ischemia and neurodegeneration. Proteomics 4(8):2234\u20132241. https:\/\/doi.org\/10.1002\/pmic.200300822","journal-title":"Proteomics"},{"issue":"3","key":"2745_CR53","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1021\/pr101123t","volume":"10","author":"L Dayon","year":"2011","unstructured":"Dayon L, Turck N, Garc\u00ed-Berrocoso T, Walter N, Burkhard PR, Vilalta A, Sahuquillo J, Montaner J et al (2011) Brain extracellular fluid protein changes in acute stroke patients. J Proteome Res 10(3):1043\u20131051. https:\/\/doi.org\/10.1021\/pr101123t","journal-title":"J Proteome Res"},{"issue":"12","key":"2745_CR54","doi-asserted-by":"publisher","first-page":"1196","DOI":"10.1038\/nbt.4016","volume":"35","author":"B Alvarez-Castelao","year":"2017","unstructured":"Alvarez-Castelao B, Schanzenb\u00e4cher CT, Hanus C, Glock C, Tom Dieck S, D\u00f6rrbaum AR, Bartnik I, Nassim-Assir B et al (2017) Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nat Biotechnol 35(12):1196\u20131201. https:\/\/doi.org\/10.1038\/nbt.4016","journal-title":"Nat Biotechnol"},{"issue":"6304","key":"2745_CR55","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.1126\/science.aag0821","volume":"353","author":"A Uezu","year":"2016","unstructured":"Uezu A, Kanak DJ, Bradshaw TW, Soderblom EJ, Catavero CM, Burette AC, Weinberg RJ, Soderling SH (2016) Identification of an elaborate complex mediating postsynaptic inhibition. Science 353(6304):1123\u20131129. https:\/\/doi.org\/10.1126\/science.aag0821","journal-title":"Science"},{"key":"2745_CR56","doi-asserted-by":"publisher","unstructured":"Wilson RS, Nairn AC (2018) Cell-type-specific proteomics: a neuroscience perspective. Proteomes 6(4):51. https:\/\/doi.org\/10.3390\/proteomes6040051","DOI":"10.3390\/proteomes6040051"},{"issue":"4","key":"2745_CR57","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1093\/brain\/awz022","volume":"142","author":"AS Tsai","year":"2019","unstructured":"Tsai AS, Berry K, Beneyto MM, Gaudilliere D, Ganio EA, Culos A, Ghaemi MS, Choisy B et al (2019) A year-long immune profile of the systemic response in acute stroke survivors. Brain 142(4):978\u2013991. https:\/\/doi.org\/10.1093\/brain\/awz022","journal-title":"Brain"},{"key":"2745_CR58","doi-asserted-by":"publisher","unstructured":"Li Y, Wang Y, Yao Y, Griffiths BB, Feng L, Tao T, Wang F, Xu B et al (2020) Systematic study of the immune components after ischemic stroke using CyTOF techniques. J Immunol Res 2020:9132410. https:\/\/doi.org\/10.1155\/2020\/9132410","DOI":"10.1155\/2020\/9132410"},{"issue":"9","key":"2745_CR59","doi-asserted-by":"publisher","first-page":"2381","DOI":"10.1074\/mcp.E113.031658","volume":"12","author":"R Aebersold","year":"2013","unstructured":"Aebersold R, Burlingame AL, Bradshaw RA (2013) Western blots versus selected reaction monitoring assays: time to turn the tables? Mol Cell Proteomics 12(9):2381\u20132382. https:\/\/doi.org\/10.1074\/mcp.E113.031658","journal-title":"Mol Cell Proteomics"},{"issue":"7","key":"2745_CR60","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1111\/j.1476-5381.2009.00211.x","volume":"157","author":"U Dirnagl","year":"2009","unstructured":"Dirnagl U, Macleod MR (2009) Stroke research at a road block: the streets from adversity should be paved with meta-analysis and good laboratory practice. Br J Pharmacol 157(7):1154\u20131156. https:\/\/doi.org\/10.1111\/j.1476-5381.2009.00211.x","journal-title":"Br J Pharmacol"},{"issue":"5","key":"2745_CR61","doi-asserted-by":"publisher","first-page":"1510","DOI":"10.1161\/STROKEAHA.113.004075","volume":"45","author":"U Dirnagl","year":"2014","unstructured":"Dirnagl U, Endres M (2014) Found in translation: Preclinical stroke research predicts human pathophysiology, clinical phenotypes, and therapeutic outcomes. Stroke 45(5):1510\u20131518. https:\/\/doi.org\/10.1161\/STROKEAHA.113.004075","journal-title":"Stroke"},{"issue":"18","key":"2745_CR62","doi-asserted-by":"publisher","first-page":"3865","DOI":"10.1002\/cne.24040","volume":"524","author":"CS von Bartheld","year":"2016","unstructured":"von Bartheld CS, Bahney J, Herculano-Houzel S (2016) The search for true numbers of neurons and glial cells in the human brain: a review of 150 years of cell counting. J Comp Neurol 524(18):3865\u20133895. https:\/\/doi.org\/10.1002\/cne.24040","journal-title":"J Comp Neurol"},{"issue":"1","key":"2745_CR63","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1186\/s13059-018-1547-5","volume":"19","author":"B Budnik","year":"2018","unstructured":"Budnik B, Levy E, Harmange G, Slavov N (2018) SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. Genome Biol 19(1):161. https:\/\/doi.org\/10.1186\/s13059-018-1547-5","journal-title":"Genome Biol"},{"key":"2745_CR64","doi-asserted-by":"publisher","first-page":"140658","DOI":"10.1016\/j.bbapap.2021.140658","volume":"7","author":"L Goto-Silva","year":"1869","unstructured":"Goto-Silva L (1869) Junqueira M (2021) Single-cell proteomics: a treasure trove in neurobiology. Biochim Biophys Acta Proteins Proteom 7:140658. https:\/\/doi.org\/10.1016\/j.bbapap.2021.140658","journal-title":"Biochim Biophys Acta Proteins Proteom"},{"key":"2745_CR65","doi-asserted-by":"publisher","first-page":"674189","DOI":"10.3389\/fimmu.2021.674189","volume":"12","author":"CC Hsiao","year":"2021","unstructured":"Hsiao CC, Sankowski R, Prinz M, Smolders J, Huitinga I, Hamann J (2021) GPCRomics of Homeostatic and disease-associated human microglia. Front Immunol 12:674189. https:\/\/doi.org\/10.3389\/fimmu.2021.674189","journal-title":"Front Immunol"},{"issue":"8 suppl 1","key":"2745_CR66","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1074\/mcp.MR118.001246","volume":"18","author":"B Vitrinel","year":"2019","unstructured":"Vitrinel B, Koh HWL, Mujgan Kar F, Maity S, Rendleman J, Choi H, Vogel C (2019) Exploiting interdata relationships in next-generation proteomics analysis. Mol Cell Proteomics 18(8 suppl 1):S5-S14. https:\/\/doi.org\/10.1074\/mcp.MR118.001246","journal-title":"Mol Cell Proteomics"},{"issue":"5","key":"2745_CR67","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1038\/s41582-020-0350-6","volume":"16","author":"J Montaner","year":"2020","unstructured":"Montaner J, Ramiro L, Simats A, Tiedt S, Makris K, Jickling GC, Debette S, Sanchez JC et al (2020) Multilevel omics for the discovery of biomarkers and therapeutic targets for stroke. Nat Rev Neurol 16(5):247\u2013264. https:\/\/doi.org\/10.1038\/s41582-020-0350-6","journal-title":"Nat Rev Neurol"}],"container-title":["Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-022-02745-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12035-022-02745-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-022-02745-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T07:06:21Z","timestamp":1650351981000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12035-022-02745-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,26]]},"references-count":67,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["2745"],"URL":"https:\/\/doi.org\/10.1007\/s12035-022-02745-2","relation":{},"ISSN":["0893-7648","1559-1182"],"issn-type":[{"value":"0893-7648","type":"print"},{"value":"1559-1182","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,26]]},"assertion":[{"value":"4 October 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 January 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 January 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"Not applicable","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}