{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:33:51Z","timestamp":1790980431886,"version":"4.1.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"TUBAP","award":["2017\/68"],"award-info":[{"award-number":["2017\/68"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Neurobiol"],"published-print":{"date-parts":[[2023,2]]},"DOI":"10.1007\/s12035-022-03135-4","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T12:04:07Z","timestamp":1668600247000},"page":"875-883","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Investigation of Relationship Between Small Noncoding RNA (sncRNA) Expression Levels and Serum Iron, Copper, and Zinc Levels in Clinical Diagnosed Multiple Sclerosis Patients"],"prefix":"10.1007","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8412-091X","authenticated-orcid":false,"given":"Arzu","family":"Ay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3745-8838","authenticated-orcid":false,"given":"Nevra","family":"Alkanli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3937-5243","authenticated-orcid":false,"given":"Engin","family":"Atli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8967-6124","authenticated-orcid":false,"given":"Hakan","family":"Gurkan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4739-9234","authenticated-orcid":false,"given":"Tevfik","family":"Gulyasar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6375-6153","authenticated-orcid":false,"given":"Sibel","family":"Guler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1057-9864","authenticated-orcid":false,"given":"Tammam","family":"Sipahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6678-482X","authenticated-orcid":false,"given":"Necdet","family":"Sut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"issue":"3","key":"3135_CR1","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1007\/s12035-012-8270-0","volume":"45","author":"C Angerstein","year":"2012","unstructured":"Angerstein C, Hecker M, Paap BK, Koczan D, Thamilarasan M, Thiesen HJ et al (2012) Integration of MicroRNA databases to study MicroRNAs associated with multiple sclerosis. Mol Neurobiol 45(3):520\u2013535. https:\/\/doi.org\/10.1007\/s12035-012-8270-0","journal-title":"Mol Neurobiol"},{"issue":"6","key":"3135_CR2","doi-asserted-by":"publisher","first-page":"2886","DOI":"10.1007\/s12035-021-02315-y","volume":"58","author":"M Hecker","year":"2021","unstructured":"Hecker M, Fitzner B, J\u00e4ger K, B\u00fchring J, Schwartz M, Hartmann A et al (2021) Leukocyte telomere length in patients with multiple sclerosis and its association with clinical phenotypes. Mol Neurobiol 58(6):2886\u20132896. https:\/\/doi.org\/10.1007\/s12035-021-02315-y","journal-title":"Mol Neurobiol"},{"issue":"8","key":"3135_CR3","doi-asserted-by":"publisher","first-page":"4651","DOI":"10.1007\/s12035-022-02854-y","volume":"59","author":"A Nowak","year":"2022","unstructured":"Nowak A, Wicik Z, Wolska M, Shahzadi A, Szwed P, Jarosz-Popek J et al (2022) The role of non-coding RNAs in neuroinflammatory process in multiple sclerosis. Mol Neurobiol 59(8):4651\u20134668. https:\/\/doi.org\/10.1007\/s12035-022-02854-y","journal-title":"Mol Neurobiol"},{"issue":"5","key":"3135_CR4","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.1007\/s11033-012-2449-3","volume":"40","author":"M Liu","year":"2013","unstructured":"Liu M, Hou X, Zhang P, Hao Y, Yang Y, Wu X et al (2013) Microarray gene expression profiling analysis combined with bioinformatics in multiple sclerosis. Mol Biol Rep 40(5):3731\u20133737","journal-title":"Mol Biol Rep"},{"issue":"10","key":"3135_CR5","doi-asserted-by":"publisher","first-page":"e7440","DOI":"10.1371\/journal.pone.0007440","volume":"4","author":"A Keller","year":"2009","unstructured":"Keller A, Leidinger P, Lange J, Borries A, Schroers H, Scheffler M et al (2009) Multiple sclerosis: microRNA expression profiles accurately differentiate patients with relapsing-remitting disease from healthy controls. PLoS ONE 4(10):e7440","journal-title":"PLoS ONE"},{"key":"3135_CR6","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.jaut.2019.04.002","volume":"101","author":"E Piket","year":"2019","unstructured":"Piket E, Zheleznyakova GY, Kular L, Jagodic M (2019) Small non-coding RNAs as important players, biomarkers and therapeutic T targets in multiple sclerosis: a comprehensive overview. J Autoimmun 101:17\u201325","journal-title":"J Autoimmun"},{"key":"3135_CR7","doi-asserted-by":"publisher","first-page":"7484","DOI":"10.1038\/s41598-020-64422-5","volume":"10","author":"Q Pilson","year":"2020","unstructured":"Pilson Q, Smith S, Jefferies CA, Gabhann-Dromgoole JN, Murphy CC (2020) miR-744-5p contributes to ocular inflammation in patients with primary Sjogrens Syndrome. Sci Rep 10:7484. https:\/\/doi.org\/10.1038\/s41598-020-64422-5","journal-title":"Sci Rep"},{"key":"3135_CR8","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1186\/s12974-015-0418-1","volume":"12","author":"A Keller","year":"2015","unstructured":"Keller A, Leidinger P, Meese E, Haas J, Backes C, Rasche L et al (2015) Next-generation sequencing identifies altered whole blood microRNAs in neuromyelitis optica spectrum disorder which may permit discrimination from multiple sclerosis. J Neuroinflammation 12:196","journal-title":"J Neuroinflammation"},{"key":"3135_CR9","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.3389\/fimmu.2018.01256","volume":"9","author":"C Chen","year":"2018","unstructured":"Chen C, Zhou Y, Wang J, Yan Y, Peng L, Qiu W (2018) Dysregulated microRNA involvement in multiple sclerosis by induction of T helper 17 cell differentiation. Front Immunol 9:1256","journal-title":"Front Immunol"},{"issue":"2","key":"3135_CR10","doi-asserted-by":"publisher","first-page":"145","DOI":"10.30958\/ajh.4-2-3","volume":"4","author":"M Janghorbani","year":"2017","unstructured":"Janghorbani M, Shaygannejad V, Hakimdavood M, Salari M (2017) Trace elements in serum samples of patients with multiple sclerosis. Athens Journal of Health 4(2):145\u2013154","journal-title":"Athens Journal of Health"},{"issue":"4","key":"3135_CR11","doi-asserted-by":"publisher","first-page":"e0122142","DOI":"10.1371\/journal.pone.0122142","volume":"10","author":"E Tamburo","year":"2015","unstructured":"Tamburo E, Varrica D, Dongarr\u00e0 G, Grimaldi LM (2015) Trace elements in scalp hair samples from patients with relapsing-remitting multiple sclerosis. PLoS ONE 10(4):e0122142","journal-title":"PLoS ONE"},{"key":"3135_CR12","first-page":"112","volume":"1","author":"Z Xu","year":"2014","unstructured":"Xu Z, Shi Z, Li Y (2014) The crosstalk between micro RNA and iron homeostasis. Int J Genomic Med 1:112","journal-title":"Int J Genomic Med"},{"issue":"3","key":"3135_CR13","doi-asserted-by":"publisher","first-page":"175909141665151","DOI":"10.1177\/1759091416651511","volume":"8","author":"M Bredholt","year":"2016","unstructured":"Bredholt M, Frederiksen JL (2016) Zinc in multiple sclerosis: a systematic review and meta-analysis. ASN Neuro 8(3):1759091416651511","journal-title":"ASN Neuro"},{"issue":"4","key":"3135_CR14","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1017\/S0029665100000781","volume":"59","author":"L Rink","year":"2000","unstructured":"Rink L, Gabriel P (2000) Zinc and the immune system. Proc Nutr Soc 59(4):541\u2013552","journal-title":"Proc Nutr Soc"},{"key":"3135_CR15","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.nut.2017.03.009","volume":"39\u201340","author":"K Socha","year":"2017","unstructured":"Socha K, Karpinska E, Kochanowicz J (2017) Dietary habits; concentration of copper, zinc, and Cu-to-Zn ratio in serum and ability status of patients with relapsing-remitting multiple sclerosis. Nutrition 39\u201340:76\u201381","journal-title":"Nutrition"},{"issue":"52","key":"3135_CR16","doi-asserted-by":"publisher","first-page":"20970","DOI":"10.1073\/pnas.1117207108","volume":"108","author":"MS Ryu","year":"2011","unstructured":"Ryu MS, Langkamp-Henken B, Chang SM, Shankar MN, Cousins RJ (2011) Genomic analysis, cytokine expression, and microRNA profiling reveal biomarkers of human dietary zinc depletion and homeostasis. Proc Natl Acad Sci U S A 108(52):20970\u201320975","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"3135_CR17","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1093\/ajcn\/50.1.136","volume":"50","author":"DK Smith","year":"1989","unstructured":"Smith DK, Feldman EB, Feldman DS (1989) The trace elements status in multiple sclerosis. Am J Clin Nutr 50(1):136\u2013140","journal-title":"Am J Clin Nutr"},{"issue":"3","key":"3135_CR18","doi-asserted-by":"publisher","first-page":"1030","DOI":"10.1111\/nph.14244","volume":"213","author":"M Pilon","year":"2017","unstructured":"Pilon M (2017) The copper microRNAs. New Phytol 213(3):1030\u20131035","journal-title":"New Phytol"},{"issue":"1","key":"3135_CR19","doi-asserted-by":"publisher","first-page":"21","DOI":"10.32607\/20758251-2016-8-1-21-33","volume":"8","author":"NM Baulina","year":"2016","unstructured":"Baulina NM, Kulakova OG, Favorova OO (2016) MicroRNAs: the role in autoimmune inflammation. Acta Naturae 8(1):21\u201333","journal-title":"Acta Naturae"},{"issue":"9","key":"3135_CR20","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1038\/ng2103","volume":"39","author":"SG Gregory","year":"2007","unstructured":"Gregory SG, Schmidt S, Seth P, Oksenberg JR, Hart J, Prokop A et al (2007) Multiple sclerosis genetics group Interleukin 7 receptor alpha chain (IL7R) shows allelic and functional association with multiple sclerosis. Nat Genet 39(9):1083\u201391","journal-title":"Nat Genet"},{"issue":"1","key":"3135_CR21","doi-asserted-by":"publisher","first-page":"68","DOI":"10.3390\/nu10010068","volume":"10","author":"A Sanna","year":"2018","unstructured":"Sanna A, Firinu D, Zavattari P, Valera P (2018) Zinc status and autoimmunity: a systematic review and meta-analysis. Nutrients 10(1):68","journal-title":"Nutrients"},{"key":"3135_CR22","unstructured":"QIAamp RNA Blood Mini Handbook, 3. Kit Contents. QIAamp RNA Blood Mini Kit. Catalog no. Preparations per Kit. 52304. 50. QIAamp Spin Columns (clear). 2021; file:\/\/\/Users\/nevraalkanli\/Downloads\/HB 0322\u2013004_HB_QA_RNA_Blood_Mini_0221_WW%20(3).pdf"},{"key":"3135_CR23","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/1471-2199-7-3","volume":"7","author":"A Schroeder","year":"2006","unstructured":"Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M et al (2006) The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 7:3","journal-title":"BMC Mol Biol"},{"key":"3135_CR24","unstructured":"miRNA microarray system with miRNA complete labeling and Hyb kit protocol. Agilent Technologies. 2021.\u00a0www.agilent.com. Accessed\u00a0Oct 2021"},{"key":"3135_CR25","unstructured":"Agilent feature extraction software, automated image analysis paired with QC tools product note. Agilent Technologies. 2016.\u00a0www.agilent.com. Accessed\u00a0Jan 2016"},{"issue":"10","key":"3135_CR26","doi-asserted-by":"publisher","first-page":"6911","DOI":"10.1007\/s11033-021-06694-2","volume":"48","author":"A Ay","year":"2021","unstructured":"Ay A, Gulyasar T, Alkanli N, Sipahi T, Cicin I, Kocak Z et al (2021) Investigation of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patients with colorectal cancer. Mol Biol Rep 48(10):6911\u20136921","journal-title":"Mol Biol Rep"},{"issue":"4","key":"3135_CR27","doi-asserted-by":"publisher","first-page":"606","DOI":"10.4103\/1673-5374.266905","volume":"15","author":"B Martinez","year":"2020","unstructured":"Martinez B, Peplow PV (2020) MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression. Neural Regen Res 15(4):606\u2013619","journal-title":"Neural Regen Res"},{"issue":"10","key":"3135_CR28","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1038\/nmeth.1682","volume":"8","author":"A Keller","year":"2011","unstructured":"Keller A, Leidinger P, Bauer A, Elsharawy A, Haas J, Backes C et al (2011) Toward the blood-borne miRNome of human diseases. Nat Methods 8(10):841\u2013843","journal-title":"Nat Methods"},{"issue":"7","key":"3135_CR29","doi-asserted-by":"publisher","first-page":"e6309","DOI":"10.1371\/journal.pone.0006309","volume":"4","author":"D Otaegui","year":"2009","unstructured":"Otaegui D, Baranzini SE, Arma\u00f1anzas R, Calvo B, Mu\u00f1oz-Culla M, Khankhanian P et al (2009) Differential micro RNA expression in PBMC from multiple sclerosis patients. PLoS ONE 4(7):e6309","journal-title":"PLoS ONE"},{"issue":"8","key":"3135_CR30","doi-asserted-by":"publisher","first-page":"e12132","DOI":"10.1371\/journal.pone.0012132","volume":"5","author":"MB Cox","year":"2010","unstructured":"Cox MB, Cairns MJ, Gandhi KS, Carroll AP, Moscovis S, Stewart GJ et al (2010) MicroRNAs miR-17 and miR-20a inhibit T cell activation genes and are under-expressed in MS whole blood. PLoS ONE 5(8):e12132. https:\/\/doi.org\/10.1371\/journal.pone.0012132","journal-title":"PLoS ONE"},{"issue":"1","key":"3135_CR31","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/nrneurol.2010.179","volume":"7","author":"A Junker","year":"2011","unstructured":"Junker A, Hohlfeld R, Meinl E (2011) The emerging role of microRNAs in multiple sclerosis. Nat Rev Neurol 7(1):56\u201359","journal-title":"Nat Rev Neurol"},{"key":"3135_CR32","doi-asserted-by":"publisher","first-page":"955461","DOI":"10.3389\/fnagi.2022.955461","volume":"14","author":"S Ghiam","year":"2020","unstructured":"Ghiam S, Eslahchi C, Shahpasand K, Habibi-Rezaei M, Gharaghani S (2020) Exploring the role of non-coding RNAs as potential candidate biomarkers in the cross-talk between diabetes mellitus and Alzheimer\u2019s disease. Front Aging Neurosci 14:955461. https:\/\/doi.org\/10.3389\/fnagi.2022.955461","journal-title":"Front Aging Neurosci"},{"issue":"1","key":"3135_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12035-020-02096-w","volume":"58","author":"MT Kabir","year":"2021","unstructured":"Kabir MT, Uddin MS, Zaman S, Begum Y, Ashraf GM, Bin-Jumah MN et al (2021) Molecular mechanisms of metal toxicity in the pathogenesis of Alzheimer\u2019s disease. Mol Neurobiol 58(1):1\u201320. https:\/\/doi.org\/10.1007\/s12035-020-02096-w","journal-title":"Mol Neurobiol"},{"issue":"3","key":"3135_CR34","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1007\/s12035-019-01832-1","volume":"57","author":"G Bj\u00f8rklund","year":"2020","unstructured":"Bj\u00f8rklund G, Dadar M, Chirumbolo S, Aaseth J (2020) The role of xenobiotics and trace metals in Parkinson\u2019s disease. Mol Neurobiol 57(3):1405\u20131417. https:\/\/doi.org\/10.1007\/s12035-019-01832-1","journal-title":"Mol Neurobiol"},{"issue":"6","key":"3135_CR35","first-page":"994","volume":"6","author":"MA Al-Zubaidi","year":"2012","unstructured":"Al-Zubaidi MA (2012) The effect of interferon Beta-1b and methylprednisolone treatment on the serum trace elements in Iraqi patients with multiple sclerosis. J Clin Diagn Res 6(6):994\u2013998","journal-title":"J Clin Diagn Res"},{"key":"3135_CR36","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1186\/s41983-019-0107-6","volume":"55","author":"MI Oraby","year":"2019","unstructured":"Oraby MI, Hussein M, Abd Elkareem R, Eman E (2019) The emerging role of serum zinc in motor disability and radiological findings in patients with multiple sclerosis. Egypt J Neurol Psychiatry Neurosurg 55:60","journal-title":"Egypt J Neurol Psychiatry Neurosurg"},{"issue":"9","key":"3135_CR37","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1016\/S0753-3322(03)00012-X","volume":"57","author":"H Tapiero","year":"2003","unstructured":"Tapiero H, Townsend DM, Tew KD (2003) Trace elements in human physiology and pathology. Copper Biomed Pharmacother 57(9):386\u2013398","journal-title":"Copper Biomed Pharmacother"},{"issue":"8","key":"3135_CR38","doi-asserted-by":"publisher","first-page":"967","DOI":"10.3390\/nu10080967","volume":"10","author":"M Pawlitzki","year":"2018","unstructured":"Pawlitzki M, Uebelh\u00f6r J, Sweeney-Reed CM (2018) Lower serum zinc levels in patients with multiple sclerosis compared to healthy controls. Nutrients 10(8):967","journal-title":"Nutrients"}],"container-title":["Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-022-03135-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12035-022-03135-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-022-03135-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T03:58:48Z","timestamp":1674100728000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12035-022-03135-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,16]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["3135"],"URL":"https:\/\/doi.org\/10.1007\/s12035-022-03135-4","relation":{},"ISSN":["0893-7648","1559-1182"],"issn-type":[{"value":"0893-7648","type":"print"},{"value":"1559-1182","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,16]]},"assertion":[{"value":"17 September 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 November 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 November 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The approval of the ethics committee was obtained from the Trakya University Faculty of Medicine Non-Invasive Ethics Committee (Protocol code: T\u00dcTF-BAEK 2017\/11).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"Signed informed consent forms were collected from each of the MS patients and healthy controls.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed Consent"}},{"value":"The author, Arzu Ay, Nevra Alkanli, Engin Atli, Hakan Gurkan, Tevfik Gulyasar, Sibel Guler, Tammam Sipahi, Necdet Sut have read and approved the final manuscript for submission. We confirm the tables and figures are original for this article.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}