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The amygdala, an almond-shaped limbic structure in the medial temporal lobe, exerts a critical effect on emotion and pain formation. However, studies on inflammatory pain-induced aversion are still relatively limited, and the available evidence is not enough to clarify its inherent mechanisms. Proteomics is a high-throughput, comprehensive, and objective study method that compares the similarities and differences of protein expression under different conditions to screen potential targets. The current study aimed to identify potential pivotal proteins in the amygdala of rats after complete Freund\u2019s adjuvant (CFA)-induced pain aversion via proteomics analysis. Immunohistochemistry was performed to confirm the expression of glutamate transporter-1 (GLT-1) in the amygdala during different periods of pain aversion. Thirteen proteins were found to be different between the day 2 and day 15 groups. Among the 13 differentially expressed proteins, Q8R64 denotes GLT-1, which utilises synaptic glutamate to remain optimal extracellular glutamic levels, thereby preventing accumulation in the synaptic cleft and consequent excitotoxicity. The variation in GLT-1 expression was correlated with the variation tendency of pain aversion, which implies a potential link between the modulation of pain aversion and the excitability of glutamatergic neurons. This study demonstrated that exposure to inflammatory pain results in aversion induced from pain, leading to extensive biological changes in the amygdala.<\/jats:p>","DOI":"10.1007\/s12035-023-03415-7","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T17:01:39Z","timestamp":1691168499000},"page":"7166-7184","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic Analysis of the Amygdala Reveals Dynamic Changes in Glutamate Transporter-1 During Progression of Complete Freund\u2019s Adjuvant-Induced Pain Aversion"],"prefix":"10.1007","volume":"60","author":[{"given":"Yuanyuan","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuerong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunyun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqin","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chalian","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zui","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofen","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4499-0352","authenticated-orcid":false,"given":"Jianqiao","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,8,4]]},"reference":[{"key":"3415_CR1","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/0304-3959(94)90167-8","volume":"56","author":"G Magni","year":"1994","unstructured":"Magni G, Moreschi C, Rigatti-Luchini S, Merskey H (1994) Prospective study on the relationship between depressive symptoms and chronic musculoskeletal pain. 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(). In Wu et al. () and this article, the authors observed consistent data regarding the paw withdrawal threshold and conditioned place aversion score in the saline group. However, the primary focus of the article by Wu et al. () was to investigate the underlying central neural mechanisms of pain aversion and the effect of electro-acupuncture. The authors\u2019 aim was to understand the functional mechanisms of electro-acupuncture. On the other hand, this article () aimed to further explore the central neural mechanisms of pain aversion. The authors specifically investigated the potential link between pain aversion modulation and the excitability of glutamate neurons. The findings indicated that pain aversion induces significant biological changes in the amygdala.Considering the distinct research objectives of both articles, the authors made the decision not to aggregate the data in order to maintain clarity regarding the research topics and perspectives of each article.\u00a0","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosure"}}]}}