{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:50:38Z","timestamp":1774975838445,"version":"3.50.1"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF14CC0001"],"award-info":[{"award-number":["NNF14CC0001"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007505","name":"Kirsten og Freddy Johansens Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008269","name":"L\u00e6ge Sophus Carl Emil Friis og hustru Olga Doris Friis' Legat","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008274","name":"P. A. Messerschmidt og Hustrus Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008274","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University College Copenhagen The Library"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Neurobiol"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract\n<\/jats:title><jats:p>The purpose of this study was to identify and validate new putative lead drug targets in drug-resistant mesial temporal lobe epilepsy (mTLE) starting from differentially expressed genes (DEGs) previously identified in mTLE in humans by transcriptome analysis. We identified consensus DEGs among two independent mTLE transcriptome datasets and assigned them status as \u201clead target\u201d if they (1) were involved in neuronal excitability, (2) were new in mTLE, and (3) were druggable. For this, we created a consensus DEG network in STRING and annotated it with information from the DISEASES database and the Target Central Resource Database (TCRD). Next, we attempted to validate lead targets using qPCR, immunohistochemistry, and Western blot on hippocampal and temporal lobe neocortical tissue from mTLE patients and non-epilepsy controls, respectively. Here we created a robust, unbiased list of 113 consensus DEGs starting from two lists of 3040 and 5523 mTLE significant DEGs, respectively, and identified five lead targets. Next, we showed that CACNB3, a voltage-gated Ca<jats:sup>2+<\/jats:sup> channel subunit, was significantly regulated in mTLE at both mRNA and protein level. Considering the key role of Ca<jats:sup>2+<\/jats:sup> currents in regulating neuronal excitability, this suggested a role for CACNB3 in seizure generation. This is the first time changes in CACNB3 expression have been associated with drug-resistant epilepsy in humans, and since efficient therapeutic strategies for the treatment of drug-resistant mTLE are lacking, our finding might represent a step toward designing such new treatment strategies.<\/jats:p>","DOI":"10.1007\/s12035-023-03426-4","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T10:01:40Z","timestamp":1687341700000},"page":"5755-5769","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Differential Expression of the \u03b23 Subunit of Voltage-Gated Ca2+ Channel in Mesial Temporal Lobe Epilepsy"],"prefix":"10.1007","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2293-2365","authenticated-orcid":false,"given":"Christina","family":"Kj\u00e6r","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oana","family":"Palasca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Barzaghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0784-7864","authenticated-orcid":false,"given":"Lasse K.","family":"Bak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00fana K. J.","family":"Durhuus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6290-4222","authenticated-orcid":false,"given":"Emil","family":"Jakobsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Pedersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8290-5772","authenticated-orcid":false,"given":"Emil D.","family":"Bartels","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6321-3850","authenticated-orcid":false,"given":"David P. D.","family":"Woldbye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9024-7936","authenticated-orcid":false,"given":"Lars H.","family":"Pinborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7885-715X","authenticated-orcid":false,"given":"Lars Juhl","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"issue":"4","key":"3426_CR1","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1111\/epi.13709","volume":"58","author":"IE Scheffer","year":"2017","unstructured":"Scheffer IE, Berkovic S, Capovilla G, Connolly MB, French J, Guilhoto L et al (2017) ILAE classification of the epilepsies: position paper of the ILAE Commission for Classification and Terminology. Epilepsia (Copenhagen) 58(4):512\u2013521. https:\/\/doi.org\/10.1111\/epi.13709","journal-title":"Epilepsia (Copenhagen)"},{"issue":"5","key":"3426_CR2","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1111\/j.1528-1167.2009.02481.x","volume":"51","author":"AK Ngugi","year":"2010","unstructured":"Ngugi AK, Bottomley C, Kleinschmidt I, Sander JW, Newton CR (2010) Estimation of the burden of active and life-time epilepsy: a meta-analytic approach. Epilepsia 51(5):883\u2013890. https:\/\/doi.org\/10.1111\/j.1528-1167.2009.02481.x","journal-title":"Epilepsia"},{"issue":"4","key":"3426_CR3","doi-asserted-by":"publisher","first-page":"e0174964","DOI":"10.1371\/journal.pone.0174964","volume":"12","author":"H Chu","year":"2017","unstructured":"Chu H, Sun P, Yin J, Liu G, Wang Y, Zhao P et al (2017) Integrated network analysis reveals potentially novel molecular mechanisms and therapeutic targets of refractory epilepsies. PLoS One. 12(4):e0174964. https:\/\/doi.org\/10.1371\/journal.pone.0174964","journal-title":"PLoS One."},{"issue":"6","key":"3426_CR4","doi-asserted-by":"publisher","first-page":"1616","DOI":"10.1093\/brain\/awz074","volume":"142","author":"S Guelfi","year":"2019","unstructured":"Guelfi S, Botia JA, Thom M, Ramasamy A, Perona M, Stanyer L et al (2019) Transcriptomic and genetic analyses reveal potential causal drivers for intractable partial epilepsy. Brain 142(6):1616\u20131630. https:\/\/doi.org\/10.1093\/brain\/awz074","journal-title":"Brain"},{"issue":"10","key":"3426_CR5","doi-asserted-by":"publisher","first-page":"e55","DOI":"10.1093\/brain\/awz265","volume":"142","author":"C Kj\u00e6r","year":"2019","unstructured":"Kj\u00e6r C, Barzaghi G, Bak LK, Goetze JP, Yde CW, Woldbye D et al (2019) Transcriptome analysis in patients with temporal lobe epilepsy. Brain. 142(10):e55. https:\/\/doi.org\/10.1093\/brain\/awz265","journal-title":"Brain."},{"issue":"1","key":"3426_CR6","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1124\/pr.117.014456","volume":"70","author":"J Oyrer","year":"2018","unstructured":"Oyrer J, Maljevic S, Scheffer IE, Berkovic SF, Petrou S, Reid CA (2018) Ion channels in genetic epilepsy: from genes and mechanisms to disease-targeted therapies. Pharmacol Rev 70(1):142\u2013173. https:\/\/doi.org\/10.1124\/pr.117.014456","journal-title":"Pharmacol Rev"},{"issue":"4","key":"3426_CR7","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1111\/epi.13670","volume":"58","author":"RS Fisher","year":"2017","unstructured":"Fisher RS, Cross JH, French JA, Higurashi N, Hirsch E, Jansen FE et al (2017) Operational classification of seizure types by the International League Against Epilepsy: position paper of the ILAE Commission for Classification and Terminology. Epilepsia (Copenhagen) 58(4):522\u2013530. https:\/\/doi.org\/10.1111\/epi.13670","journal-title":"Epilepsia (Copenhagen)"},{"issue":"6","key":"3426_CR8","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1111\/j.1528-1167.2009.02397.x","volume":"51","author":"P Kwan","year":"2010","unstructured":"Kwan P, Arzimanoglou A, Berg AT, Brodie MJ, Allen Hauser W, Mathern G et al (2010) Definition of drug resistant epilepsy: consensus proposal by the ad hoc Task Force of the ILAE Commission on Therapeutic Strategies. Epilepsia 51(6):1069\u20131077. https:\/\/doi.org\/10.1111\/j.1528-1167.2009.02397.x","journal-title":"Epilepsia"},{"issue":"12","key":"3426_CR9","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/0165-6147(94)90059-0","volume":"15","author":"N Upton","year":"1994","unstructured":"Upton N (1994) Mechanisms of action of new antiepileptic drugs: rational design and serendipitous findings. Trends Pharmacol Sci 15(12):456\u2013463. https:\/\/doi.org\/10.1016\/0165-6147(94)90059-0","journal-title":"Trends Pharmacol Sci"},{"issue":"23","key":"3426_CR10","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1016\/j.drudis.2011.09.007","volume":"16","author":"I Gashaw","year":"2011","unstructured":"Gashaw I, Ellinghaus P, Sommer A, Asadullah K (2011) What makes a good drug target? Drug Discovery Today 16(23):1037\u20131043. https:\/\/doi.org\/10.1016\/j.drudis.2011.09.007","journal-title":"Drug Discovery Today"},{"issue":"4","key":"3426_CR11","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1513\/pats.200601-011TK","volume":"3","author":"N Kaminski","year":"2006","unstructured":"Kaminski N, Rosas IO (2006) Gene expression profiling as a window into idiopathic pulmonary fibrosis pathogenesis: can we identify the right target genes? Proc Am Thorac Soc 3(4):339\u2013344. https:\/\/doi.org\/10.1513\/pats.200601-011TK","journal-title":"Proc Am Thorac Soc"},{"issue":"1","key":"3426_CR12","doi-asserted-by":"publisher","first-page":"Ns20160003","DOI":"10.1042\/NS20160003","volume":"1","author":"N Weiss","year":"2017","unstructured":"Weiss N, Zamponi GW (2017) Trafficking of neuronal calcium channels. Neuronal Signal. 1(1):Ns20160003. https:\/\/doi.org\/10.1042\/NS20160003","journal-title":"Neuronal Signal."},{"key":"3426_CR13","doi-asserted-by":"publisher","unstructured":"Schampel A, Kuerten S (2017) Danger: high voltage-the role of voltage-gated calcium channels in central nervous system Pathology. Cells 6(4). https:\/\/doi.org\/10.3390\/cells6040043,\u00a0https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5755501\/","DOI":"10.3390\/cells6040043"},{"issue":"2","key":"3426_CR14","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1021\/acs.jproteome.8b00702","volume":"18","author":"NT Doncheva","year":"2019","unstructured":"Doncheva NT, Morris JH, Gorodkin J, Jensen LJ (2019) Cytoscape StringApp: network analysis and visualization of proteomics data. J Proteome Res 18(2):623\u2013632. https:\/\/doi.org\/10.1021\/acs.jproteome.8b00702","journal-title":"J Proteome Res"},{"issue":"D1","key":"3426_CR15","doi-asserted-by":"publisher","first-page":"D607","DOI":"10.1093\/nar\/gky1131","volume":"47","author":"D Szklarczyk","year":"2019","unstructured":"Szklarczyk D, Gable AL, Lyon D, Junge A, Wyder S, Huerta-Cepas J et al (2019) STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res 47(D1):D607\u2013D613. https:\/\/doi.org\/10.1093\/nar\/gky1131","journal-title":"Nucleic Acids Res"},{"issue":"D1","key":"3426_CR16","doi-asserted-by":"publisher","first-page":"D1334","DOI":"10.1093\/nar\/gkaa993","volume":"49","author":"TK Sheils","year":"2021","unstructured":"Sheils TK, Mathias SL, Kelleher KJ, Siramshetty VB, Nguyen DT, Bologa CG et al (2021) TCRD and Pharos 2021: mining the human proteome for disease biology. Nucleic Acids Res 49(D1):D1334\u2013D1346. https:\/\/doi.org\/10.1093\/nar\/gkaa993","journal-title":"Nucleic Acids Res"},{"key":"3426_CR17","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.ymeth.2014.11.020","volume":"74","author":"S Pletscher-Frankild","year":"2015","unstructured":"Pletscher-Frankild S, Pallej\u00e0 A, Tsafou K, Binder JX, Jensen LJ (2015) DISEASES: text mining and data integration of disease-gene associations. Methods 74:83\u201389. https:\/\/doi.org\/10.1016\/j.ymeth.2014.11.020","journal-title":"Methods"},{"key":"3426_CR18","doi-asserted-by":"publisher","unstructured":"Stelzer G, Rosen N, Plaschkes I, Zimmerman S, Twik M, Fishilevich S et al (2016) The GeneCards Suite: from gene data mining to disease genome sequence analyses. Curr Protoc Bioinformatics 54:1.30.1\u20131..3. https:\/\/doi.org\/10.1002\/cpbi.5","DOI":"10.1002\/cpbi.5"},{"issue":"4","key":"3426_CR19","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1007\/s12264-017-0134-1","volume":"33","author":"F Wei","year":"2017","unstructured":"Wei F, Yan LM, Su T, He N, Lin ZJ, Wang J et al (2017) Ion channel genes and epilepsy: functional alteration, pathogenic potential, and mechanism of epilepsy. Neurosci Bull 33(4):455\u2013477. https:\/\/doi.org\/10.1007\/s12264-017-0134-1","journal-title":"Neurosci Bull"},{"key":"3426_CR20","doi-asserted-by":"publisher","first-page":"81","DOI":"10.3389\/fncel.2016.00081","volume":"10","author":"C Villa","year":"2016","unstructured":"Villa C, Combi R (2016) Potassium channels and human epileptic phenotypes: an updated overview. Front Cell Neurosci 10:81. https:\/\/doi.org\/10.3389\/fncel.2016.00081","journal-title":"Front Cell Neurosci"},{"key":"3426_CR21","unstructured":"Patel A, Biso G, Fowler JB (2021) Neuroanatomy, temporal lobe. StatPearls. Treasure Island (FL): StatPearls Publishing Copyright \u00a9 2021, StatPearls Publishing LLC.\u00a0https:\/\/pubmed.ncbi.nlm.nih.gov\/30137797\/"},{"issue":"3","key":"3426_CR22","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.pharmthera.2004.10.004","volume":"105","author":"RJ Delorenzo","year":"2005","unstructured":"Delorenzo RJ, Sun DA, Deshpande LS (2005) Cellular mechanisms underlying acquired epilepsy: the calcium hypothesis of the induction and maintenance of epilepsy. Pharmacol Ther 105(3):229\u2013266. https:\/\/doi.org\/10.1016\/j.pharmthera.2004.10.004","journal-title":"Pharmacol Ther"},{"issue":"1","key":"3426_CR23","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.seizure.2009.10.006","volume":"19","author":"T Gholipour","year":"2010","unstructured":"Gholipour T, Ghasemi M, Riazi K, Ghaffarpour M, Dehpour AR (2010) Seizure susceptibility alteration through 5-HT 3 receptor: modulation by nitric oxide. Seizure 19(1):17\u201322. https:\/\/doi.org\/10.1016\/j.seizure.2009.10.006","journal-title":"Seizure"},{"issue":"D1","key":"3426_CR24","doi-asserted-by":"publisher","first-page":"D995","DOI":"10.1093\/nar\/gkw1072","volume":"45","author":"D-T Nguyen","year":"2017","unstructured":"Nguyen D-T, Mathias S, Bologa C, Brunak S, Fernandez N, Gaulton A et al (2017) Pharos: collating protein information to shed light on the druggable genome. Nucleic Acids Res 45(D1):D995\u2013D1002. https:\/\/doi.org\/10.1093\/nar\/gkw1072","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"3426_CR25","doi-asserted-by":"publisher","first-page":"e0203760-e","DOI":"10.1371\/journal.pone.0203760","volume":"13","author":"KA Jones","year":"2018","unstructured":"Jones KA, Luo Y, Dukes-Rimsky L, Srivastava DP, Koul-Tewari R, Russell TA et al (2018) Neurodevelopmental disorder-associated ZBTB20 gene variants affect dendritic and synaptic structure. PloS one. 13(10):e0203760-e. https:\/\/doi.org\/10.1371\/journal.pone.0203760","journal-title":"PloS one."},{"issue":"Pt 9","key":"3426_CR26","doi-asserted-by":"publisher","first-page":"2336","DOI":"10.1093\/brain\/aww202","volume":"139","author":"N Bernasconi","year":"2016","unstructured":"Bernasconi N (2016) Is epilepsy a curable neurodegenerative disease? Brain 139(Pt 9):2336\u20132337. https:\/\/doi.org\/10.1093\/brain\/aww202","journal-title":"Brain"},{"key":"3426_CR27","unstructured":"Torben M, Morten M\u00f8ller F (2010) Basal neuroanatomi. 3. udgave. ed. Moos T, M\u00f8ller M, editors. Kbh: FADL.\u00a0https:\/\/bibliotek.dk\/da\/work\/870970-basis%3A28205171"},{"issue":"3","key":"3426_CR28","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1001\/jamaneurol.2017.3949","volume":"75","author":"Z Chen","year":"2018","unstructured":"Chen Z, Brodie MJ, Liew D, Kwan P (2018) Treatment outcomes in patients with newly diagnosed epilepsy treated with established and new antiepileptic drugs: a 30-year longitudinal cohort study. JAMA Neurol 75(3):279\u2013286. https:\/\/doi.org\/10.1001\/jamaneurol.2017.3949","journal-title":"JAMA Neurol"},{"issue":"Suppl 3","key":"3426_CR29","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1111\/j.1528-1167.2010.02602.x","volume":"51","author":"A Pitk\u00e4nen","year":"2010","unstructured":"Pitk\u00e4nen A (2010) Therapeutic approaches to epileptogenesis\u2013hope on the horizon. Epilepsia. 51(Suppl 3):2\u201317. https:\/\/doi.org\/10.1111\/j.1528-1167.2010.02602.x","journal-title":"Epilepsia."},{"issue":"1","key":"3426_CR30","doi-asserted-by":"publisher","first-page":"5038","DOI":"10.1038\/s41467-020-18752-7","volume":"11","author":"U Pfisterer","year":"2020","unstructured":"Pfisterer U, Petukhov V, Demharter S, Meichsner J, Thompson Jonatan J, BatiukMykhailo Y et al (2020) Identification of epilepsy-associated neuronal subtypes and gene expression underlying epileptogenesis. Nature communications 11(1):5038. https:\/\/doi.org\/10.1038\/s41467-020-18752-7","journal-title":"Nature communications"},{"issue":"4","key":"3426_CR31","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1152\/physrev.00057.2009","volume":"90","author":"Z Buraei","year":"2010","unstructured":"Buraei Z, Yang J (2010) The \u00df subunit of voltage-gated Ca2+ channels. Physiol Rev 90(4):1461\u20131506. https:\/\/doi.org\/10.1152\/physrev.00057.2009","journal-title":"Physiol Rev"},{"issue":"1","key":"3426_CR32","doi-asserted-by":"publisher","first-page":"a022723","DOI":"10.1101\/cshperspect.a022723","volume":"6","author":"S Rajakulendran","year":"2016","unstructured":"Rajakulendran S, Hanna MG (2016) The role of calcium channels in epilepsy. Cold Spring Harb Perspect Med 6(1):a022723. https:\/\/doi.org\/10.1101\/cshperspect.a022723","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"5","key":"3426_CR33","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1016\/j.celrep.2018.01.010","volume":"22","author":"A Belkacemi","year":"2018","unstructured":"Belkacemi A, Hui X, Wardas B, Laschke MW, Wissenbach U, Menger MD et al (2018) IP(3) Receptor-dependent cytoplasmic Ca(2+) signals are tightly controlled by Cav\u03b23. Cell Rep 22(5):1339\u20131349. https:\/\/doi.org\/10.1016\/j.celrep.2018.01.010","journal-title":"Cell Rep"},{"issue":"Pt 3","key":"3426_CR34","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1113\/jphysiol.2005.093195","volume":"567","author":"S Dalton","year":"2005","unstructured":"Dalton S, Takahashi SX, Miriyala J, Colecraft HM (2005) A single CaVbeta can reconstitute both trafficking and macroscopic conductance of voltage-dependent calcium channels. J Physiol 567(Pt 3):757\u2013769. https:\/\/doi.org\/10.1113\/jphysiol.2005.093195","journal-title":"J Physiol"},{"key":"3426_CR35","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.seizure.2022.11.007","volume":"103","author":"X Zhou","year":"2022","unstructured":"Zhou X, Chen Z, Xiao L, Zhong Y, Liu Y, Wu J et al (2022) Intracellular calcium homeostasis and its dysregulation underlying epileptic seizures. Seizure 103:126\u2013136. https:\/\/doi.org\/10.1016\/j.seizure.2022.11.007","journal-title":"Seizure"},{"issue":"18","key":"3426_CR36","doi-asserted-by":"publisher","first-page":"12093","DOI":"10.1074\/jbc.M800816200.Journalarticle","volume":"283","author":"D Jeon","year":"2008","unstructured":"Jeon D, Song I, Guido W, Kim K, Kim E, Oh U et al (2008) Ablation of Ca2+ channel beta3 subunit leads to enhanced N-methyl-D-aspartate receptor-dependent long term potentiation and improved long term memory. J Biol Chem 283(18):12093\u201312101. https:\/\/doi.org\/10.1074\/jbc.M800816200.Journalarticle","journal-title":"J Biol Chem"},{"issue":"9","key":"3426_CR37","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1016\/j.biopsych.2016.08.040","volume":"82","author":"KJE van Hulzen","year":"2017","unstructured":"van Hulzen KJE, Scholz CJ, Franke B, Ripke S, Klein M, McQuillin A et al (2017) Genetic overlap between attention-deficit\/hyperactivity disorder and bipolar disorder: evidence from genome-wide association study meta-analysis. Biol Psychiatry 82(9):634\u2013641. https:\/\/doi.org\/10.1016\/j.biopsych.2016.08.040","journal-title":"Biol Psychiatry"},{"issue":"1","key":"3426_CR38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/s0169-328x(99)00141-2","volume":"71","author":"F Lin","year":"1999","unstructured":"Lin F, Barun S, Lutz CM, Wang Y, Hosford DA (1999) Decreased (45)Ca(2)(+) uptake in P\/Q-type calcium channels in homozygous lethargic (Cacnb4lh) mice is associated with increased beta3 and decreased beta4 calcium channel subunit mRNA expression. Brain Res Mol Brain Res 71(1):1\u201310. https:\/\/doi.org\/10.1016\/s0169-328x(99)00141-2","journal-title":"Brain Res Mol Brain Res"},{"key":"3426_CR39","doi-asserted-by":"publisher","first-page":"10775","DOI":"10.1038\/srep10775","volume":"5","author":"A Koussounadis","year":"2015","unstructured":"Koussounadis A, Langdon SP, Um IH, Harrison DJ, Smith VA (2015) Relationship between differentially expressed mRNA and mRNA-protein correlations in a xenograft model system. Sci Rep 5:10775. https:\/\/doi.org\/10.1038\/srep10775","journal-title":"Sci Rep"},{"issue":"12","key":"3426_CR40","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.1039\/b908315d","volume":"5","author":"AR de Sousa","year":"2009","unstructured":"de Sousa AR, Penalva LO, Marcotte EM, Vogel C (2009) Global signatures of protein and mRNA expression levels. Mol Biosyst 5(12):1512\u20131526. https:\/\/doi.org\/10.1039\/b908315d","journal-title":"Mol Biosyst"},{"issue":"4","key":"3426_CR41","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1038\/nrg3185","volume":"13","author":"C Vogel","year":"2012","unstructured":"Vogel C, Marcotte EM (2012) Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat Rev Genet 13(4):227\u2013232. https:\/\/doi.org\/10.1038\/nrg3185","journal-title":"Nat Rev Genet"},{"issue":"3","key":"3426_CR42","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.tig.2015.01.001","volume":"31","author":"ET de Klerk","year":"2015","unstructured":"de Klerk ET, Hoen PA (2015) Alternative mRNA transcription, processing, and translation: insights from RNA sequencing. Trends Genet 31(3):128\u201339. https:\/\/doi.org\/10.1016\/j.tig.2015.01.001","journal-title":"Trends Genet"},{"issue":"7","key":"3426_CR43","doi-asserted-by":"publisher","first-page":"2139","DOI":"10.1093\/brain\/awaa168","volume":"143","author":"A Parras","year":"2020","unstructured":"Parras A, de Diego-Garcia L, Alves M, Beamer E, Conte G, Jimenez-Mateos EM et al (2020) Polyadenylation of mRNA as a novel regulatory mechanism of gene expression in temporal lobe epilepsy. Brain 143(7):2139\u20132153. https:\/\/doi.org\/10.1093\/brain\/awaa168","journal-title":"Brain"},{"issue":"1","key":"3426_CR44","doi-asserted-by":"publisher","first-page":"2524","DOI":"10.1038\/s41467-019-10513-5","volume":"10","author":"W Shao","year":"2019","unstructured":"Shao W, Guo T, Toussaint NC, Xue P, Wagner U, Li L et al (2019) Comparative analysis of mRNA and protein degradation in prostate tissues indicates high stability of proteins. Nat Commun 10(1):2524. https:\/\/doi.org\/10.1038\/s41467-019-10513-5","journal-title":"Nat Commun"},{"key":"3426_CR45","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/1741-7007-12-42","volume":"12","author":"I Gallego Romero","year":"2014","unstructured":"Gallego Romero I, Pai AA, Tung J, Gilad Y (2014) RNA-seq: impact of RNA degradation on transcript quantification. BMC Biol 12:42. https:\/\/doi.org\/10.1186\/1741-7007-12-42","journal-title":"BMC Biol"},{"issue":"3","key":"3426_CR46","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1007\/s11064-020-03183-z","volume":"46","author":"PSH Jensen","year":"2021","unstructured":"Jensen PSH, Johansen M, Bak LK, Jensen LJ, Kj\u00e6r C (2021) Yield and integrity of RNA from brain samples are largely unaffected by pre-analytical procedures. Neurochem Res 46(3):447\u2013454. https:\/\/doi.org\/10.1007\/s11064-020-03183-z","journal-title":"Neurochem Res"},{"issue":"41","key":"3426_CR47","doi-asserted-by":"publisher","first-page":"eabn8367","DOI":"10.1126\/sciadv.abn8367","volume":"8","author":"MY Batiuk","year":"2022","unstructured":"Batiuk MY, Tyler T, Dragicevic K, Mei S, Rydbirk R, Petukhov V et al (2022) Upper cortical layer-driven network impairment in schizophrenia. Sci Adv 8(41):eabn8367. https:\/\/doi.org\/10.1126\/sciadv.abn8367","journal-title":"Sci Adv"},{"key":"3426_CR48","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.brainres.2009.10.019","volume":"1308","author":"P N'Gouemo","year":"2010","unstructured":"N\u2019Gouemo P, Yasuda R, Faingold CL (2010) Seizure susceptibility is associated with altered protein expression of voltage-gated calcium channel subunits in inferior colliculus neurons of the genetically epilepsy-prone rat. Brain Res 1308:153\u2013157. https:\/\/doi.org\/10.1016\/j.brainres.2009.10.019","journal-title":"Brain Res"},{"issue":"2","key":"3426_CR49","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/s0306-4522(99)00162-1","volume":"93","author":"AA Lie","year":"1999","unstructured":"Lie AA, Bl\u00fcmcke I, Volsen SG, Wiestler OD, Elger CE, Beck H (1999) Distribution of voltage-dependent calcium channel beta subunits in the hippocampus of patients with temporal lobe epilepsy. Neuroscience 93(2):449\u2013456. https:\/\/doi.org\/10.1016\/s0306-4522(99)00162-1","journal-title":"Neuroscience"},{"key":"3426_CR50","doi-asserted-by":"publisher","unstructured":"B\u00e9k\u00e9s M, Langley DR, Crews CM (2022) PROTAC targeted protein degraders: the past is prologue. Nat Rev Drug Discov 1\u201320. https:\/\/doi.org\/10.1038\/s41573-021-00371-6,\u00a0https:\/\/www.nature.com\/articles\/s41573-021-00371-6","DOI":"10.1038\/s41573-021-00371-6"},{"issue":"2","key":"3426_CR51","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.eplepsyres.2006.09.008","volume":"73","author":"CP Taylor","year":"2007","unstructured":"Taylor CP, Angelotti T, Fauman E (2007) Pharmacology and mechanism of action of pregabalin: the calcium channel alpha2-delta (alpha2-delta) subunit as a target for antiepileptic drug discovery. Epilepsy Res 73(2):137\u2013150. https:\/\/doi.org\/10.1016\/j.eplepsyres.2006.09.008","journal-title":"Epilepsy Res"},{"key":"3426_CR52","doi-asserted-by":"publisher","first-page":"107966","DOI":"10.1016\/j.neuropharm.2020.107966","volume":"168","author":"GJ Sills","year":"2020","unstructured":"Sills GJ, Rogawski MA (2020) Mechanisms of action of currently used antiseizure drugs. Neuropharmacology. 168:107966. https:\/\/doi.org\/10.1016\/j.neuropharm.2020.107966","journal-title":"Neuropharmacology."},{"issue":"6220","key":"3426_CR53","doi-asserted-by":"publisher","first-page":"1260419","DOI":"10.1126\/science.1260419","volume":"347","author":"M Uhl\u00e9n","year":"2015","unstructured":"Uhl\u00e9n M, Fagerberg L, Hallstr\u00f6m BM, Lindskog C, Oksvold P, Mardinoglu A et al (2015) Proteomics. Tissue-based map of the human proteome. Science 347(6220):1260419. https:\/\/doi.org\/10.1126\/science.1260419","journal-title":"Science"}],"container-title":["Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-023-03426-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12035-023-03426-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-023-03426-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T03:55:15Z","timestamp":1693540515000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12035-023-03426-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,21]]},"references-count":53,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["3426"],"URL":"https:\/\/doi.org\/10.1007\/s12035-023-03426-4","relation":{},"ISSN":["0893-7648","1559-1182"],"issn-type":[{"value":"0893-7648","type":"print"},{"value":"1559-1182","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,21]]},"assertion":[{"value":"21 December 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 June 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 June 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The use of resected mTLE patient tissue, non-epilepsy control subject tissues from brain banks, and following procedures were approved by the local Ethical Committee in Copenhagen (H-2\u20132011-104). Written informed consent was obtained from all subjects prior to each surgery.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval and Consent to Participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}