{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T19:15:29Z","timestamp":1758050129702,"version":"3.44.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T00:00:00Z","timestamp":1749254400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T00:00:00Z","timestamp":1749254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074059"],"award-info":[{"award-number":["82074059"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Anhui Province Academic Leader Reserve Candidate Funding Project","award":["2022H287"],"award-info":[{"award-number":["2022H287"]}]},{"name":"Anhui Provincial Health Research Key Project","award":["AHWJ2022a013"],"award-info":[{"award-number":["AHWJ2022a013"]}]},{"name":"Anhui Province College Natural Science Research Key Project","award":["2023AH050745"],"award-info":[{"award-number":["2023AH050745"]}]},{"name":"Anhui Province Hygiene and Health Outstanding Talents Project","award":["ahsjhmypygc20230074"],"award-info":[{"award-number":["ahsjhmypygc20230074"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Neurobiol"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1007\/s12035-025-05131-w","type":"journal-article","created":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T15:03:30Z","timestamp":1749308610000},"page":"13073-13088","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Role of Microglia Activation in Ischemia Stroke Based on the circDnajc1\/miR-27a-5p\/C1qc Signaling Axis"],"prefix":"10.1007","volume":"62","author":[{"given":"Shuangping","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingping","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujun","family":"Xue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiyin","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizhong","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianchun","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,6,7]]},"reference":[{"key":"5131_CR1","doi-asserted-by":"publisher","unstructured":"Zhang Y, Shen L, Xie J, Li L, Xi W, Li B, Bai Y, Yao H et al (2023) Pushen capsule treatment promotes functional recovery after ischemic stroke. Phytomed\u00a0 Int J Phytother Phytopharmacol 111:154664. https:\/\/doi.org\/10.1016\/j.phymed.2023.154664","DOI":"10.1016\/j.phymed.2023.154664"},{"key":"5131_CR2","doi-asserted-by":"publisher","unstructured":"Tian D, Pan Y, Zhao Y, Wang H, Tian Y, Yang L, Shi W, Zhang C et al (2023) TCR\u03b1\u03b2(+)NK1.1(-)CD4(-)CD8(-) double-negative T cells inhibit central and peripheral inflammation and ameliorate ischemic stroke in mice. Theranostics 13(3):896\u2013909. https:\/\/doi.org\/10.7150\/thno.80307","DOI":"10.7150\/thno.80307"},{"key":"5131_CR3","doi-asserted-by":"publisher","first-page":"116507","DOI":"10.1016\/j.jep.2023.116507","volume":"312","author":"S Zhao","year":"2023","unstructured":"Zhao S, Zhang P, Yan Y, Xu W, Li J, Wang L, Wang N, Huang Y (2023) Network pharmacology-based prediction and validation of the active ingredients and potential mechanisms of the Huangxiong formula for treating ischemic stroke. J Ethnopharmacol 312:116507. https:\/\/doi.org\/10.1016\/j.jep.2023.116507","journal-title":"J Ethnopharmacol"},{"key":"5131_CR4","doi-asserted-by":"publisher","unstructured":"Alhazmi H, Bani-Sadr A, Bochaton T, Paccalet A, Da Silva CC, Buisson M, Amaz C, Ameli R et al (2021) Large vessel cardioembolic stroke and embolic stroke of undetermined source share a common profile of matrix metalloproteinase-9 level and susceptibility vessel sign length. Eur J Neurol 28(6):1977\u20131983. https:\/\/doi.org\/10.1111\/ene.14806","DOI":"10.1111\/ene.14806"},{"key":"5131_CR5","doi-asserted-by":"publisher","unstructured":"Cai W, Hu M, Li C, Wu R, Lu D, Xie C, Zhang W, Li T et al (2023) FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation. Autophagy 19(4):1144\u20131163. https:\/\/doi.org\/10.1080\/15548627.2022.2116833","DOI":"10.1080\/15548627.2022.2116833"},{"key":"5131_CR6","doi-asserted-by":"publisher","first-page":"9991001","DOI":"10.1155\/2021\/9991001","volume":"2021","author":"Y Zhou","year":"2021","unstructured":"Zhou Y, Liao J, Mei Z, Liu X, Ge J (2021) Insight into crosstalk between ferroptosis and necroptosis: novel therapeutics in ischemic stroke. Oxid Med Cell Longev 2021:9991001. https:\/\/doi.org\/10.1155\/2021\/9991001","journal-title":"Oxid Med Cell Longev"},{"issue":"1","key":"5131_CR7","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1186\/s10020-021-00312-4","volume":"27","author":"M Da","year":"2021","unstructured":"Da M, Zhuang J, Zhou Y, Qi Q, Han S (2021) Role of long noncoding RNA taurine-upregulated gene 1 in cancers. Molecular medicine (Cambridge, Mass) 27(1):51. https:\/\/doi.org\/10.1186\/s10020-021-00312-4","journal-title":"Mol Med (Cambridge, Mass)"},{"issue":"8","key":"5131_CR8","doi-asserted-by":"publisher","first-page":"e0039721","DOI":"10.1128\/mcb.00397-21","volume":"42","author":"ZT Guo","year":"2022","unstructured":"Guo ZT, Yu Q, Miao C, Ge W, Li P (2022) MicroRNA-27a-5p inhibits proliferation, migration, and invasion and promotes apoptosis of Wilms\u2019 tumor cell by targeting PBOV1. Mol Cell Biol 42(8):e0039721. https:\/\/doi.org\/10.1128\/mcb.00397-21","journal-title":"Mol Cell Biol"},{"key":"5131_CR9","doi-asserted-by":"publisher","unstructured":"Yang T, Wang D, Qu Y, Wang Y, Feng Y, Yang Y, Luo Q, Sun X et al (2020) N-hydroxy-N\u2019-(4-butyl-2-methylphenyl)-formamidine attenuates oxygen-glucose deprivation and reoxygenation-induced cerebral ischemia-reperfusion injury via regulation of microRNAs. J Integr Neurosci 19(2):303\u2013311. https:\/\/doi.org\/10.31083\/j.jin.2020.02.1236","DOI":"10.31083\/j.jin.2020.02.1236"},{"issue":"12","key":"5131_CR10","doi-asserted-by":"publisher","first-page":"e1010744","DOI":"10.1371\/journal.pcbi.1010744","volume":"18","author":"Y Jiang","year":"2022","unstructured":"Jiang Y, Zhang JX, Liu R (2022) Systematic comparison of differential expression networks in MTB mono-, HIV mono- and MTB\/HIV co-infections for drug repurposing. PLoS Comput Biol 18(12):e1010744. https:\/\/doi.org\/10.1371\/journal.pcbi.1010744","journal-title":"PLoS Comput Biol"},{"issue":"Pt 1","key":"5131_CR11","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1042\/bst0320021","volume":"32","author":"RB Sim","year":"2004","unstructured":"Sim RB, Tsiftsoglou SA (2004) Proteases of the complement system. Biochem Soc Trans 32(Pt 1):21\u201327. https:\/\/doi.org\/10.1042\/bst0320021","journal-title":"Biochem Soc Trans"},{"key":"5131_CR12","doi-asserted-by":"publisher","unstructured":"Zhu H, Jian Z, Zhong Y, Ye Y, Zhang Y, Hu X, Pu B, Gu L et al (2021) Janus kinase inhibition ameliorates ischemic stroke injury and neuroinflammation through reducing NLRP3 inflammasome activation via JAK2\/STAT3 pathway inhibition. Front Immunol 12:714943. https:\/\/doi.org\/10.3389\/fimmu.2021.714943","DOI":"10.3389\/fimmu.2021.714943"},{"key":"5131_CR13","doi-asserted-by":"publisher","unstructured":"Zhang Z, Zou X, Zhang R, Xie Y, Feng Z, Li F, Han J, Sun H et al (2021) Human umbilical cord mesenchymal stem cell-derived exosomal miR-146a-5p reduces microglial-mediated neuroinflammation via suppression of the IRAK1\/TRAF6 signaling pathway after ischemic stroke. Aging 13(2):3060\u20133079. https:\/\/doi.org\/10.18632\/aging.202466","DOI":"10.18632\/aging.202466"},{"key":"5131_CR14","doi-asserted-by":"publisher","unstructured":"Liu M, Xu Z, Wang L, Zhang L, Liu Y, Cao J, Fu Q, Liu Y et al (2020) Cottonseed oil alleviates ischemic stroke injury by inhibiting the inflammatory activation of microglia and astrocyte. J Neuroinflammation 17(1):270. https:\/\/doi.org\/10.1186\/s12974-020-01946-7","DOI":"10.1186\/s12974-020-01946-7"},{"key":"5131_CR15","doi-asserted-by":"publisher","unstructured":"Zhao Y, Zhang X, Chen X, Wei Y (2022) Neuronal injuries in cerebral infarction and ischemic stroke: from mechanisms to treatment (review). Int J Mol Med 49(2). https:\/\/doi.org\/10.3892\/ijmm.2021.5070","DOI":"10.3892\/ijmm.2021.5070"},{"key":"5131_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.pneurobio.2015.12.006","volume":"138\u2013140","author":"O Lazarov","year":"2016","unstructured":"Lazarov O, Hollands C (2016) Hippocampal neurogenesis: learning to remember. Prog Neurobiol 138\u2013140:1\u201318. https:\/\/doi.org\/10.1016\/j.pneurobio.2015.12.006","journal-title":"Prog Neurobiol"},{"key":"5131_CR17","doi-asserted-by":"publisher","first-page":"376","DOI":"10.3389\/fphar.2018.00376","volume":"9","author":"E Kossatz","year":"2018","unstructured":"Kossatz E, Silva-Pe\u00f1a D, Su\u00e1rez J, de Fonseca FR, Maldonado R, Robledo P (2018) Octadecylpropyl sulfamide reduces neurodegeneration and restores the memory deficits induced by hypoxia-ischemia in mice. Front Pharmacol 9:376. https:\/\/doi.org\/10.3389\/fphar.2018.00376","journal-title":"Front Pharmacol"},{"key":"5131_CR18","doi-asserted-by":"publisher","unstructured":"Chen R, Zhu H, Wang Z, Zhang Y, Wang J, Huang Y, Gu L, Li C et al (2023) Targeting microglia\/macrophages Notch1 protects neurons from pyroptosis in ischemic stroke. Brain Sci 13(12). https:\/\/doi.org\/10.3390\/brainsci13121657","DOI":"10.3390\/brainsci13121657"},{"key":"5131_CR19","doi-asserted-by":"publisher","unstructured":"Ji ZJ, Shi Y, Li X, Hou R, Yang Y, Liu ZQ, Duan XC, Liu Q et al (2022) Neuroprotective effect of Taohong Siwu decoction on cerebral ischemia\/reperfusion injury via mitophagy-NLRP3 inflammasome pathway. Front Pharmacol 13:910217. https:\/\/doi.org\/10.3389\/fphar.2022.910217","DOI":"10.3389\/fphar.2022.910217"},{"key":"5131_CR20","doi-asserted-by":"publisher","unstructured":"Zhu YM, Lin L, Wei C, Guo Y, Qin Y, Li ZS, Kent TA, McCoy CE et al (2021) The key regulator of necroptosis, RIP1 kinase, contributes to the formation of astrogliosis and glial scar in ischemic stroke. Transl Stroke Res 12(6):991\u20131017. https:\/\/doi.org\/10.1007\/s12975-021-00888-3","DOI":"10.1007\/s12975-021-00888-3"},{"issue":"8","key":"5131_CR21","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1016\/s1875-5364(20)30074-1","volume":"18","author":"FF Chen","year":"2020","unstructured":"Chen FF, Wang MM, Xia WW, Peng DY, Han L (2020) Tao-Hong-Si-Wu decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles. Chin J Nat Med 18(8):620\u2013627. https:\/\/doi.org\/10.1016\/s1875-5364(20)30074-1","journal-title":"Chin J Nat Med"},{"key":"5131_CR22","doi-asserted-by":"publisher","unstructured":"Li C, Zhao Z, Luo Y, Ning T, Liu P, Chen Q, Chu Y, Guo Q et al (2021) Macrophage-disguised manganese dioxide nanoparticles for neuroprotection by reducing oxidative stress and modulating inflammatory microenvironment in acute ischemic stroke. Adv Sci (Weinheim, Baden-Wurttemberg, Germany) 8(20):e2101526. https:\/\/doi.org\/10.1002\/advs.202101526","DOI":"10.1002\/advs.202101526"},{"key":"5131_CR23","doi-asserted-by":"publisher","unstructured":"Liao YC, Wang JW, Guo C, Bai M, Ran Z, Wen LM, Ju BW, Ding Y et al (2023) Cistanche tubulosa alleviates ischemic stroke-induced blood-brain barrier damage by modulating microglia-mediated neuroinflammation. J Ethnopharmacol 309:116269. https:\/\/doi.org\/10.1016\/j.jep.2023.116269","DOI":"10.1016\/j.jep.2023.116269"},{"issue":"1","key":"5131_CR24","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1177\/0271678x211026770","volume":"42","author":"K Zheng","year":"2022","unstructured":"Zheng K, Lin L, Jiang W, Chen L, Zhang X, Zhang Q, Ren Y, Hao J (2022) Single-cell RNA-seq reveals the transcriptional landscape in ischemic stroke. Journal of cerebral blood flow and metabolism\u202f: official journal of the International Society of Cerebral Blood Flow and Metabolism 42(1):56\u201373. https:\/\/doi.org\/10.1177\/0271678x211026770","journal-title":"J Cereb Blood Flow Metab Off J Int Soc Cereb Blood Flow Metab"},{"key":"5131_CR25","doi-asserted-by":"publisher","unstructured":"Zhang J, Qiu W, Ma J, Wang Y, Hu Z, Long K, Wang X, Jin L et al (2019) miR-27a-5p attenuates hypoxia-induced rat cardiomyocyte injury by inhibiting Atg7. Int J Mol Sci 20(10). https:\/\/doi.org\/10.3390\/ijms20102418","DOI":"10.3390\/ijms20102418"},{"key":"5131_CR26","doi-asserted-by":"publisher","unstructured":"Cheng Y, Du L, Jiao H, Zhu H, Xu K, Guo S, Shi Q, Zhao T et al (2015) Mmu-miR-27a-5p-dependent upregulation of MCPIP1 inhibits the inflammatory response in LPS-induced RAW264.7 macrophage cells. BioMed Res Int 2015:607692. https:\/\/doi.org\/10.1155\/2015\/607692","DOI":"10.1155\/2015\/607692"},{"issue":"1","key":"5131_CR27","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1186\/s13075-021-02605-9","volume":"23","author":"T Liu","year":"2021","unstructured":"Liu T, Yang M, Xia Y, Jiang C, Li C, Jiang Z, Wang X (2021) Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther 23(1):223. https:\/\/doi.org\/10.1186\/s13075-021-02605-9","journal-title":"Arthritis Res Ther"},{"issue":"1","key":"5131_CR28","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1089\/neur.2023.0024","volume":"4","author":"M Chen","year":"2023","unstructured":"Chen M, Edwards SR, Maskey D, Woodruff TM, Tomlinson S, Reutens D (2023) Complement Component 5 (C5) deficiency improves cognitive outcome after traumatic brain injury and enhances treatment effects of complement inhibitors C1-Inh and CR2-Crry in a mouse model. Neurotrauma reports 4(1):663\u2013681. https:\/\/doi.org\/10.1089\/neur.2023.0024","journal-title":"Neurotrauma Rep"}],"container-title":["Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-025-05131-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12035-025-05131-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-025-05131-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T19:02:12Z","timestamp":1757790132000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12035-025-05131-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,7]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["5131"],"URL":"https:\/\/doi.org\/10.1007\/s12035-025-05131-w","relation":{},"ISSN":["0893-7648","1559-1182"],"issn-type":[{"type":"print","value":"0893-7648"},{"type":"electronic","value":"1559-1182"}],"subject":[],"published":{"date-parts":[[2025,6,7]]},"assertion":[{"value":"8 October 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 June 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal experiments have been approved by the Experimental Animal Ethics Committee of Anhui University of Chinese Medicine (license number: LLSC20160036).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval and Consent to Participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}