{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T04:37:11Z","timestamp":1764391031562,"version":"3.46.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100008191","name":"Wings for Life","doi-asserted-by":"publisher","award":["Scholarship Arriero-Caba\u00f1ero"],"award-info":[{"award-number":["Scholarship Arriero-Caba\u00f1ero"]}],"id":[{"id":"10.13039\/100008191","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministerio de Trabajo y Econom\u00eda Social, Spain","award":["Investigo"],"award-info":[{"award-number":["Investigo"]}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2021-128446NB-I00"],"award-info":[{"award-number":["PID2021-128446NB-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Neurobiol"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1007\/s12035-025-05508-x","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T04:33:07Z","timestamp":1764390787000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cellular Distribution of Pyruvate Kinase M2 After Spinal Cord Injury in Adult Rats"],"prefix":"10.1007","volume":"63","author":[{"given":"Alejandro","family":"Arriero-Caba\u00f1ero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"D\u00edaz-Rubio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Talab\u00e1n-Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Romero-Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinnitsa","family":"Buzoianu-Anguiano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Mey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"5508_CR1","doi-asserted-by":"crossref","unstructured":"Chen J, Chen J, Yu C, Xia K, Yang B, Wang R, et al. Metabolic reprogramming: a new option for the treatment of spinal cord injury. Neural Regen Res [Internet]. 2025;20:1042\u201357. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/38989936","DOI":"10.4103\/NRR.NRR-D-23-01604"},{"key":"5508_CR2","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjr030","author":"Z Wang","year":"2012","unstructured":"Wang Z, Chatterjee D, Jeon HY, Akerman M, Vander Heiden MG, Cantley LC et al (2012) Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons. J Mol Cell Biol. https:\/\/doi.org\/10.1093\/jmcb\/mjr030","journal-title":"J Mol Cell Biol"},{"key":"5508_CR3","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1186\/s12974-021-02337-2","volume":"18","author":"DJ Hellenbrand","year":"2021","unstructured":"Hellenbrand DJ, Quinn CM, Piper ZJ, Morehouse CN, Fixel JA (2021) Hanna AS (2021) Inflammation after spinal cord injury: a review of the critical timeline of signaling cues and cellular infiltration. J Neuroinflammation. 18:284. https:\/\/doi.org\/10.1186\/s12974-021-02337-2","journal-title":"J Neuroinflammation."},{"key":"5508_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.772205","author":"X Sheng","year":"2021","unstructured":"Sheng X, Zhao J, Li M, Xu Y, Zhou Y, Xu J et al (2021) Bone marrow mesenchymal stem cell-derived exosomes accelerate functional recovery after spinal cord injury by promoting the phagocytosis of macrophages to clean myelin debris. Front Cell Dev Biol. https:\/\/doi.org\/10.3389\/fcell.2021.772205","journal-title":"Front Cell Dev Biol"},{"key":"5508_CR5","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1084101","author":"RC Sterner","year":"2023","unstructured":"Sterner RC, Sterner RM (2023) Immune response following traumatic spinal cord injury: pathophysiology and therapies. Front Immunol. https:\/\/doi.org\/10.3389\/fimmu.2022.1084101","journal-title":"Front Immunol"},{"key":"5508_CR6","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.cmet.2014.12.005","volume":"21","author":"EM Palsson-Mcdermott","year":"2015","unstructured":"Palsson-Mcdermott EM, Curtis AM, Goel G, Lauterbach MAR, Sheedy FJ, Gleeson LE et al (2015) Pyruvate kinase M2 regulates hif-1\u03b1 activity and il-1\u03b2 induction and is a critical determinant of the warburg effect in LPS-activated macrophages. Cell Metab 21:65\u201380.\u00a0https:\/\/doi.org\/10.1016\/j.cmet.2015.01.017","journal-title":"Cell Metab"},{"key":"5508_CR7","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1038\/cr.2016.159","volume":"27","author":"J Liang","year":"2017","unstructured":"Liang J, Cao R, Wang X, Zhang Y, Wang P, Gao H et al (2017) Mitochondrial PKM2 regulates oxidative stress-induced apoptosis by stabilizing Bcl2. Cell Res 27:329\u2013351","journal-title":"Cell Res"},{"key":"5508_CR8","doi-asserted-by":"publisher","first-page":"2609","DOI":"10.1080\/15384101.2015.1064203","volume":"14","author":"J Zhang","year":"2015","unstructured":"Zhang J, Feng G, Bao G, Xu G, Sun Y, Li W et al (2015) Nuclear translocation of PKM2 modulates astrocyte proliferation via p27 and \u03b2-catenin pathway after spinal cord injury. Cell Cycle 14:2609\u20132618","journal-title":"Cell Cycle"},{"key":"5508_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-05408-3","author":"L Romero-Ram\u00edrez","year":"2022","unstructured":"Romero-Ram\u00edrez L, Garc\u00eda-Rama C, Wu S, Mey J (2022) Bile acids attenuate PKM2 pathway activation in proinflammatory microglia. Sci Rep. https:\/\/doi.org\/10.1038\/s41598-022-05408-3","journal-title":"Sci Rep"},{"key":"5508_CR10","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1016\/j.cell.2012.07.018","volume":"150","author":"W Yang","year":"2012","unstructured":"Yang W, Xia Y, Hawke D, Li X, Liang J, Xing D et al (2012) PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. Cell 150:685\u2013696","journal-title":"Cell"},{"key":"5508_CR11","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10071501","author":"S Wu","year":"2022","unstructured":"Wu S, Garc\u00eda-Rama C, Romero-Ram\u00edrez L, de Munter JPJM, Wolters EC, Kramer BW et al (2022) Tauroursodeoxycholic acid reduces neuroinflammation but does not support long term functional recovery of rats with spinal cord injury. Biomedicines. https:\/\/doi.org\/10.3390\/biomedicines10071501","journal-title":"Biomedicines"},{"key":"5508_CR12","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15727","author":"A Smaling","year":"2023","unstructured":"Smaling A, Romero-Ram\u00edrez L, Mey J, John Wiley and Sons Inc (2023) Is TGR5 a therapeutic target for the treatment of spinal cord injury? J Neurochem. https:\/\/doi.org\/10.1111\/jnc.15727","journal-title":"J Neurochem"},{"key":"5508_CR13","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2009.1177","author":"W Tetzlaff","year":"2011","unstructured":"Tetzlaff W, Okon EB, Karimi-Abdolrezaee S, Hill CE, Sparling JS, Plemel JR et al (2011) A systematic review of cellular transplantation therapies for spinal cord injury. J Neurotrauma. https:\/\/doi.org\/10.1089\/neu.2009.1177","journal-title":"J Neurotrauma"},{"key":"5508_CR14","doi-asserted-by":"publisher","unstructured":"Kvistad CE, Kr\u00e5kenes T, Gjerde C, Mustafa K, Rekand T, B\u00f8 L (2022) Safety and clinical efficacy of mesenchymal stem cell treatment in traumatic spinal cord injury, multiple sclerosis and ischemic stroke \u2013 A Systematic Review and Meta-Analysis. Front Neurol, 13.\u00a0 https:\/\/doi.org\/10.3389\/fneur.2022.891514","DOI":"10.3389\/fneur.2022.891514"},{"key":"5508_CR15","doi-asserted-by":"publisher","first-page":"319","DOI":"10.22603\/ssrr.2022-0234","volume":"7","author":"R Hirota","year":"2023","unstructured":"Hirota R, Sasaki M, Honmou O (2023) Yamashita T (2023) Mesenchymal Stem Cell Transplantation for Spinal Cord Injury: Current Status and Prospects. Spine Surg Relat Res. 7:319\u2013326. https:\/\/doi.org\/10.22603\/ssrr.2022-0234","journal-title":"Spine Surg Relat Res."},{"key":"5508_CR16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25031548","author":"M Aguado-Garrido","year":"2024","unstructured":"Aguado-Garrido M, Garc\u00eda-Rama C, Romero-Ram\u00edrez L, Buzoianu-Anguiano V, P\u00e9rez-Rizo E, Kramer BW et al (2024) Improved efficacy of delayed treatment with human bone marrow-derived stromal cells evaluated in rats with spinal cord injury. Int J Mol Sci. https:\/\/doi.org\/10.3390\/ijms25031548","journal-title":"Int J Mol Sci"},{"key":"5508_CR17","doi-asserted-by":"publisher","first-page":"5002","DOI":"10.1007\/s12035-023-03901-y","volume":"61","author":"X Zhang","year":"2024","unstructured":"Zhang X, Lei Y, Zhou H, Liu H (2024) Xu P (2024) The Role of PKM2 in Multiple Signaling Pathways Related to Neurological Diseases. Mol Neurobiol. 61:5002\u20135026. https:\/\/doi.org\/10.1007\/s12035-023-03901-y","journal-title":"Mol Neurobiol."},{"key":"5508_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-020-00629-y","author":"L Romero-Ram\u00edrez","year":"2020","unstructured":"Romero-Ram\u00edrez L, Wu S, De Munter J, Wolters EC, Kramer BW, Mey J (2020) Treatment of rats with spinal cord injury using human bone marrow-derived stromal cells prepared by negative selection. J Biomed Sci. https:\/\/doi.org\/10.1186\/s12929-020-00629-y","journal-title":"J Biomed Sci"},{"key":"5508_CR19","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4444","author":"D Han","year":"2015","unstructured":"Han D, Wei W, Chen X, Zhang Y, Wang Y, Zhang J et al (2015) NF-\u03baB\/RelA-PKM2 mediates inhibition of glycolysis by fenofibrate in glioblastoma cells. Oncotarget. https:\/\/doi.org\/10.18632\/oncotarget.4444","journal-title":"Oncotarget"},{"key":"5508_CR20","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-019-02784-7","author":"Q Wu","year":"2019","unstructured":"Wu Q, Ge W, Chen Y, Kong X, Xian H (2019) PKM2 involved in neuronal apoptosis on hypoxic-ischemic encephalopathy in neonatal rats. Neurochem Res. https:\/\/doi.org\/10.1007\/s11064-019-02784-7","journal-title":"Neurochem Res"},{"key":"5508_CR21","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1016\/j.cmet.2022.07.014","volume":"34","author":"L Traxler","year":"2022","unstructured":"Traxler L, Herdy JR, Stefanoni D, Eichhorner S, Pelucchi S, Sz\u00fccs A et al (2022) Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer\u2019s disease. Cell Metab 34:1248-1263.e6","journal-title":"Cell Metab"},{"key":"5508_CR22","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1038\/s42255-024-01049-0","volume":"6","author":"D Jimenez-Blasco","year":"2024","unstructured":"Jimenez-Blasco D, Agulla J, Lapresa R, Garcia-Macia M, Bobo-Jimenez V, Garcia-Rodriguez D et al (2024) Weak neuronal glycolysis sustains cognition and organismal fitness. Nat Metab 6:1253\u20131267","journal-title":"Nat Metab"},{"key":"5508_CR23","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113479","author":"J Gao","year":"2022","unstructured":"Gao J, Yao M, Zhang W, Yang B, Yuan G, Liu J et al (2022) Panax notoginseng saponins alleviates inflammation induced by microglial activation and protects against ischemic brain injury via inhibiting HIF-1\u03b1\/PKM2\/STAT3 signaling. Biomed Pharmacother. https:\/\/doi.org\/10.1016\/j.biopha.2022.113479","journal-title":"Biomed Pharmacother"},{"key":"5508_CR24","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04864-z","author":"J Wang","year":"2022","unstructured":"Wang J, Xu L, Lin W, Yao Y, Li H, Shen G et al (2022) Single-cell transcriptome analysis reveals the immune heterogeneity and the repopulation of microglia by Hif1\u03b1 in mice after spinal cord injury. Cell Death Dis. https:\/\/doi.org\/10.1038\/s41419-022-04864-z","journal-title":"Cell Death Dis"},{"key":"5508_CR25","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.748573","author":"Z Liu","year":"2021","unstructured":"Liu Z, Le Y, Chen H, Zhu J, Lu D, Frontiers Media S.A. (2021) Role of PKM2-mediated immunometabolic reprogramming on development of cytokine storm. Front Immunol. https:\/\/doi.org\/10.3389\/fimmu.2021.748573","journal-title":"Front Immunol"},{"key":"5508_CR26","doi-asserted-by":"publisher","first-page":"1894","DOI":"10.1073\/pnas.0914845107","volume":"107","author":"CV Clower","year":"2010","unstructured":"Clower CV, Chatterjee D, Wang Z, Cantley LC, Heidena MGV (2010) Krainer AR (2010) The alternative splicing repressors hnRNP A1\/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism. Proc Natl Acad Sci U S A. 107:1894\u20131899. https:\/\/doi.org\/10.1073\/pnas.0914845107","journal-title":"Proc Natl Acad Sci U S A."},{"key":"5508_CR27","doi-asserted-by":"publisher","DOI":"10.1038\/nature08697","author":"CJ David","year":"2010","unstructured":"David CJ, Chen M, Assanah M, Canoll P, Manley JL (2010) HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. https:\/\/doi.org\/10.1038\/nature08697","journal-title":"Nature"},{"key":"5508_CR28","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4227-7","author":"E Bowler","year":"2018","unstructured":"Bowler E, Porazinski S, Uzor S, Thibault P, Durand M, Lapointe E et al (2018) Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells. BMC Cancer. https:\/\/doi.org\/10.1186\/s12885-018-4227-7","journal-title":"BMC Cancer"},{"key":"5508_CR29","doi-asserted-by":"publisher","DOI":"10.3390\/biom12020277","author":"VN Babenko","year":"2022","unstructured":"Babenko VN, Shishkina GT, Lanshakov DA, Sukhareva EV, Dygalo NN (2022) LPS administration impacts glial immune programs by alternative splicing. Biomolecules. https:\/\/doi.org\/10.3390\/biom12020277","journal-title":"Biomolecules"},{"key":"5508_CR30","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1016\/j.cell.2011.03.054","volume":"145","author":"W Luo","year":"2011","unstructured":"Luo W, Hu H, Chang R, Zhong J, Knabel M, O\u2019Meally R et al (2011) (2011) Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell. 145:732\u2013744. https:\/\/doi.org\/10.1016\/j.cell.2011.03.054","journal-title":"Cell."},{"key":"5508_CR31","doi-asserted-by":"publisher","first-page":"361","DOI":"10.2337\/db18-0035","volume":"68","author":"Q Kong","year":"2019","unstructured":"Kong Q, Li N, Cheng H, Zhang X, Cao X, Qi T et al (2019) HSPA12A is a novel player in nonalcoholic steatohepatitis via promoting nuclear PKM2-mediated M1 macrophage polarization. Diabetes 68:361\u2013376","journal-title":"Diabetes"},{"key":"5508_CR32","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aay9217","author":"SM Seki","year":"2020","unstructured":"Seki SM, Posyniak K, McCloud R, Rosen DA, Fern\u00e1ndez-Casta\u00f1eda A, Beiter RM et al (2020) Modulation of PKM activity affects the differentiation of TH17 cells. Sci Signal. https:\/\/doi.org\/10.1126\/scisignal.aay9217","journal-title":"Sci Signal"},{"issue":"8","key":"5508_CR33","doi-asserted-by":"publisher","first-page":"2712","DOI":"10.1016\/j.celrep.2020.02.007","volume":"30","author":"K Das Gupta","year":"2020","unstructured":"Das Gupta K, Shakespear MR, Curson JEB, Murthy AMV, Iyer A, Hodson MP et al (2020) Class IIa histone deacetylases drive Toll-like receptor-inducible glycolysis and macrophage inflammatory responses via pyruvate kinase M2. Cell Rep 30(8):2712-2728.e8","journal-title":"Cell Rep"},{"key":"5508_CR34","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1007\/s11481-024-10138-6","volume":"19","author":"H Zhu","year":"2024","unstructured":"Zhu H, Zhang H, Zhao X-J, Zhang L, Liu X, Zhang Z-Y et al (2024) Tetramerization of PKM2 alleviates traumatic brain injury by ameliorating mitochondrial damage in microglia. J Neuroimmune Pharmacol 19:48","journal-title":"J Neuroimmune Pharmacol"},{"key":"5508_CR35","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13280","author":"M Xie","year":"2016","unstructured":"Xie M, Yu Y, Kang R, Zhu S, Yang L, Zeng L et al (2016) PKM2-dependent glycolysis promotes NLRP3 and AIM2 inflammasome activation. Nat Commun. https:\/\/doi.org\/10.1038\/ncomms13280","journal-title":"Nat Commun"},{"key":"5508_CR36","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25235533","author":"LY El-Sharkawy","year":"2020","unstructured":"El-Sharkawy LY, Brough D, Freeman S (2020) Inhibiting the NLRP3 inflammasome. Molecules. https:\/\/doi.org\/10.3390\/molecules25235533","journal-title":"Molecules"},{"key":"5508_CR37","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-05051-w","author":"J Zhao","year":"2022","unstructured":"Zhao J, Wang G, Han K, Wang Y, Wang L, Gao J et al (2022) Mitochondrial PKM2 deacetylation by procyanidin B2-induced SIRT3 upregulation alleviates lung ischemia\/reperfusion injury. Cell Death Dis. https:\/\/doi.org\/10.1038\/s41419-022-05051-w","journal-title":"Cell Death Dis"}],"container-title":["Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-025-05508-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12035-025-05508-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12035-025-05508-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T04:33:09Z","timestamp":1764390789000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12035-025-05508-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,29]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["5508"],"URL":"https:\/\/doi.org\/10.1007\/s12035-025-05508-x","relation":{},"ISSN":["0893-7648","1559-1182"],"issn-type":[{"value":"0893-7648","type":"print"},{"value":"1559-1182","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,29]]},"assertion":[{"value":"26 February 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of the Hospital Nacional de Parapl\u00e9jicos and approved by the regulatory authority of\n                      Castilla-La Mancha\n                      (ref. 210498, following EU directive 2010\/63\/EU for the protection of animals used for scientific purposes).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"204"}}