{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T14:41:24Z","timestamp":1778769684682,"version":"3.51.4"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2016,7,16]],"date-time":"2016-07-16T00:00:00Z","timestamp":1468627200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Genet"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1007\/s12041-016-0664-8","type":"journal-article","created":{"date-parts":[[2016,7,16]],"date-time":"2016-07-16T08:34:03Z","timestamp":1468658043000},"page":"565-572","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Characterization and expression analyses of the H+-pyrophosphatase gene in rye"],"prefix":"10.1007","volume":"95","author":[{"given":"CHANG-SHUI","family":"WANG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"QIAN-TAO","family":"JIANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JIAN","family":"MA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XIU-YING","family":"WANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JI-RUI","family":"WANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GUO-YUE","family":"CHEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PENG-FEI","family":"QI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YUAN-YING","family":"PENG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XIU-JIN","family":"LAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YOU-LIANG","family":"ZHENG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YU-MING","family":"WEI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,7,16]]},"reference":[{"key":"664_CR1","first-page":"301","volume":"1364","author":"M Baltscheffsky","year":"1998","unstructured":"Baltscheffsky M., Nadanaciva S. and Schultz A. 1998 A pyrophosphate synthase gene: molecular cloning and sequencing of the cDNA encoding the inorganic pyrophosphate synthase from Rhodospirillum rubrum. BBA-Biomembranes 1364, 301\u2013306.","journal-title":"BBA-Biomembranes"},{"key":"664_CR2","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.plantsci.2008.10.009","volume":"176","author":"AK Bao","year":"2009","unstructured":"Bao A. K., Wang S. M., Wu G. Q., Xi J. J., Zhang J. L. and Wang C. M. 2009 Overexpression of the Arabidopsis H +-PPase enhanced resistance to salt and drought stress in transgenic alfalfa (Medicago sativa L.) Plant Sci. 176, 232\u2013240.","journal-title":"Plant Sci."},{"key":"664_CR3","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/BF00197051","volume":"188","author":"C Bremberger","year":"1992","unstructured":"Bremberger C. and L\u00fcttge U. 1992 Dynamics of tonoplast proton pumps and other tonoplast proteins of Mesembryanthemum crystallinum L. during the induction of Crassulacean acid metabolism. Planta 188, 575\u2013580.","journal-title":"Planta"},{"key":"664_CR4","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.plaphy.2005.02.010","volume":"43","author":"F Brini","year":"2005","unstructured":"Brini F., Gaxiola R. A., Berkowitz G. A. and Masmoudi K. 2005 Cloning and characterization of a wheat vacuolar cation\/proton antiporter and pyrophosphatase proton pump. Plant Physiol. Biochem. 43, 347\u2013354.","journal-title":"Plant Physiol. Biochem."},{"key":"664_CR5","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1104\/pp.108.2.641","volume":"108","author":"GD Carystinos","year":"1995","unstructured":"Carystinos G. D., MacDonald H. R., Monroy A. F., Dhindsa R. S. and Poole R. J. 1995 Vacuolar H +-translocating pyrophosphatase is induced by anoxia or chilling in seedlings of rice. Plant Physiol. 108, 641\u2013649.","journal-title":"Plant Physiol."},{"key":"664_CR6","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1104\/pp.76.2.490","volume":"76","author":"KA Churchill","year":"1984","unstructured":"Churchill K. A. and Sze H. 1984 Anion-sensitive, H +-pumping ATPase of oat roots: direct effects of Cl \u2212, NO 3 \u2212 $_{\\mathrm {3}}^{\\mathrm {-}}$ , and a disulfonic stilbene. Plant Physiol. 76, 490\u2013497.","journal-title":"Plant Physiol."},{"key":"664_CR7","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/S0176-1617(11)80969-7","volume":"142","author":"R Colombo","year":"1993","unstructured":"Colombo R. and Cerana R. 1993 Enhanced activity of tonoplast pyrophosphatase in NaCl-grown cells of Daucus carota. J. Plant Physiol. 142, 226\u2013229.","journal-title":"J. Plant Physiol."},{"key":"664_CR8","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1105\/tpc.7.7.859","volume":"7","author":"NM Crawford","year":"1995","unstructured":"Crawford N. M. 1995 Nitrate: nutrient and signal for plant growth. Plant Cell 7, 859.","journal-title":"Plant Cell"},{"key":"664_CR9","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1104\/pp.109.2.659","volume":"109","author":"CP Darley","year":"1995","unstructured":"Darley C. P., Davies J. M. and Sanders D. 1995 Chill-induced changes in the activity and abundance of the vacuolar proton-pumping pyrophosphatase from mung bean hypocotyls. Plant Physiol. 109, 659\u2013665.","journal-title":"Plant Physiol."},{"key":"664_CR10","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00709-013-0528-5","volume":"251","author":"SS Dhawan","year":"2014","unstructured":"Dhawan S. S. and Sharma A. 2014 Analysis of differentially expressed genes in abiotic stress response and their role in signal transduction pathways. Protoplasma 251, 81\u201391.","journal-title":"Protoplasma"},{"key":"664_CR11","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00709-007-0268-5","volume":"232","author":"XG Duan","year":"2007","unstructured":"Duan X. G., Yang A. F., Gao F., Zhang S. L. and Zhang J. R. 2007 Heterologous expression of vacuolar H +-PPase enhances the electrochemical gradient across the vacuolar membrane and improves tobacco cell salt tolerance. Protoplasma 232, 87\u201395.","journal-title":"Protoplasma"},{"key":"664_CR12","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.2136\/sssaj1971.03615995003500060040x","volume":"35","author":"O Engelstad","year":"1971","unstructured":"Engelstad O. and Allen S. 1971 Ammonium pyrophosphate and ammonium orthophosphate as phosphorus sources: effects of soil temperature, placement, and incubation. Soil Sci. Soc. Am. J. 35, 1002\u20131004.","journal-title":"Soil Sci. Soc. Am. J."},{"key":"664_CR13","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1093\/jxb\/erh070","volume":"55","author":"A Fukuda","year":"2004","unstructured":"Fukuda A., Chiba K., Maeda M., Nakamura A., Maeshima M. and Tanaka Y. 2004 Effect of salt and osmotic stresses on the expression of genes for the vacuolar H +-pyrophosphatase, H +-ATPase subunit A, and Na +\/H + antiporter from barley. J. Exp. Bot. 55, 585\u2013594.","journal-title":"J. Exp. Bot."},{"key":"664_CR14","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1073\/pnas.96.4.1480","volume":"96","author":"RA Gaxiola","year":"1999","unstructured":"Gaxiola R. A., Rao R., Sherman A., Grisafi P., Alper S. L. and Fink G. R. 1999 The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast. Proc. Natl. Acad. Sci. USA 96, 1480\u20131485.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"664_CR15","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1042\/bj3510281","volume":"351","author":"J Hill","year":"2000","unstructured":"Hill J., Scott D., Luo S. and Docampo R. 2000 Cloning and functional expression of a gene encoding a vacuolar-type proton-translocating pyrophosphatase from Trypanosoma cruzi. Biochem. J. 351, 281\u2013288.","journal-title":"Biochem. J."},{"key":"664_CR16","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1093\/oxfordjournals.pcp.a029450","volume":"39","author":"M Kasai","year":"1998","unstructured":"Kasai M., Nakamura T., Kudo N., Sato H., Maeshima M. and Sawada S. 1998 The activity of the root vacuolar H +-pyrophosphatase in rye plants grown under conditions deficient in mineral nutrients. Plant Cell Physiol. 39, 890\u2013894.","journal-title":"Plant Cell Physiol."},{"key":"664_CR17","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jplph.2004.07.007","volume":"162","author":"SY Kuo","year":"2005","unstructured":"Kuo S. Y., Chien L. F., Hsiao Y. Y., Van R. C., Yan K. H., Liu P. F. et al. 2005 Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings. J. Plant Physiol. 162, 129\u2013138.","journal-title":"J. Plant Physiol."},{"key":"664_CR18","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/BF00041172","volume":"29","author":"J Lerchl","year":"1995","unstructured":"Lerchl J., K\u00f6nig S., Zrenner R. and Sonnewald U. 1995 Molecular cloning, characterization and expression analysis of isoforms encoding tonoplast-bound proton-translocating inorganic pyrophosphatase in tobacco. Plant Mol. Biol. 29, 833\u2013840.","journal-title":"Plant Mol. Biol."},{"key":"664_CR19","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1467-7652.2007.00301.x","volume":"6","author":"B Li","year":"2008","unstructured":"Li B., Wei A., Song C., Li N. and Zhang J. 2008 Heterologous expression of the TsVP gene improves the drought resistance of maize. Plant Biotechnol. J. 6, 146\u2013159.","journal-title":"Plant Biotechnol. J."},{"key":"664_CR20","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1126\/science.1115711","volume":"310","author":"J Li","year":"2005","unstructured":"Li J., Yang H., Peer W. A., Richter G., Blakeslee J., Bandyopadhyay A. et al. 2005 Arabidopsis H +-PPase AVP1 regulates auxin-mediated organ development. Science 310, 121\u2013125.","journal-title":"Science"},{"key":"664_CR21","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1093\/jxb\/ert442","volume":"65","author":"X Li","year":"2014","unstructured":"Li X., Guo C., Gu J., Duan W., Zhao M., Ma C. et al. 2014 Overexpression of VP, a vacuolar H +-pyrophosphatase gene in wheat (Triticum aestivum L.), improves tobacco plant growth under Pi and N deprivation, high salinity, and drought. J. Exp. Bot. 65, 683\u2013696.","journal-title":"J. Exp. Bot."},{"key":"664_CR22","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/j.1365-3040.2009.02080.x","volume":"33","author":"Z Li","year":"2010","unstructured":"Li Z., Baldwin C. M., Hu Q., Liu H. and Luo H. 2010 Heterologous expression of Arabidopsis H +pyrophosphatase enhances salt tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.) Plant Cell Environ. 33, 272\u2013289.","journal-title":"Plant Cell Environ."},{"key":"664_CR23","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/nature10963","volume":"484","author":"SM Lin","year":"2012","unstructured":"Lin S. M., Tsai J. Y., Hsiao C. D., Huang Y. T., Chiu C. L., Liu M. H. et al. 2012 Crystal structure of a membrane-embedded H +-translocating pyrophosphatase. Nature 484, 399\u2013403.","journal-title":"Nature"},{"key":"664_CR24","doi-asserted-by":"crossref","first-page":"649","DOI":"10.2144\/000112601","volume":"43","author":"YG Liu","year":"2007","unstructured":"Liu Y. G. and Chen Y. L. 2007 High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. BioTechniques 43, 649\u2013656.","journal-title":"BioTechniques"},{"key":"664_CR25","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0176-1617(11)81925-5","volume":"146","author":"A Long","year":"1995","unstructured":"Long A., Williams L. E., Nelson S. and Hall J. 1995 Localization of membrane pyrophosphatase activity in Ricinus communis seedlings. J. Plant Physiol. 146, 629\u2013638.","journal-title":"J. Plant Physiol."},{"key":"664_CR26","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1093\/pcp\/pcn090","volume":"49","author":"S Lv","year":"2008","unstructured":"Lv S., Zhang K., Gao Q., Lian L., Song Y. and Zhang J. 2008 Overexpression of an H +-PPase gene from Thellungiella halophila in cotton enhances salt tolerance and improves growth and photosynthetic performance. Plant Cell Physiol. 49, 1150\u20131164.","journal-title":"Plant Cell Physiol."},{"key":"664_CR27","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0005-2736(00)00130-9","volume":"1465","author":"M Maeshima","year":"2000","unstructured":"Maeshima M. 2000 Vacuolar H +-pyrophosphatase. BBA-Biomembranes 1465, 37\u201351.","journal-title":"BBA-Biomembranes"},{"key":"664_CR28","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0014-5793(00)02400-5","volume":"488","author":"N Mitsuda","year":"2001","unstructured":"Mitsuda N., Enami K., Nakata M., Takeyasu K. and Sato M. H. 2001 Novel type Arabidopsis thaliana H +-PPase is localized to the Golgi apparatus. FEBS Lett. 488, 29\u201333.","journal-title":"FEBS Lett."},{"key":"664_CR29","doi-asserted-by":"crossref","first-page":"4321","DOI":"10.1093\/nar\/8.19.4321","volume":"8","author":"M Murray","year":"1980","unstructured":"Murray M. and Thompson W. F. 1980 Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8, 4321\u20134326.","journal-title":"Nucleic Acids Res."},{"key":"664_CR30","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1104\/pp.116.2.589","volume":"116","author":"Y Nakanishi","year":"1998","unstructured":"Nakanishi Y. and Maeshima M. 1998 Molecular cloning of vacuolar H +-pyrophosphatase and its developmental expression in growing hypocotyl of mung bean. Plant Physiol. 116, 589\u2013597.","journal-title":"Plant Physiol."},{"key":"664_CR31","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0005-2736(96)00130-7","volume":"1284","author":"G Obermeyer","year":"1996","unstructured":"Obermeyer G., Sommer A. and Bentrup F. W. 1996 Potassium and voltage dependence of the inorganic pyrophosphatase of intact vacuoles from Chenopodium rubrum. BBA-Biomembranes 1284, 203\u2013212.","journal-title":"BBA-Biomembranes"},{"key":"664_CR32","doi-asserted-by":"crossref","first-page":"18830","DOI":"10.1073\/pnas.0509512102","volume":"102","author":"S Park","year":"2005","unstructured":"Park S., Li J., Pittman J. K., Berkowitz G. A., Yang H., Undurraga S. et al. 2005 Up-regulation of a H +-pyrophosphatase (H +-PPase) as a strategy to engineer drought-resistant crop plants. Proc. Natl. Acad. Sci. USA 102, 18830\u201318835.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"664_CR33","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1467-7652.2010.00535.x","volume":"9","author":"V Pasapula","year":"2011","unstructured":"Pasapula V., Shen G., Kuppu S., PaezValencia J., Mendoza M., Hou P. et al. 2011 Expression of an Arabidopsis vacuolar H +pyrophosphatase gene (AVP1) in cotton improves drought and salt tolerance and increases fibre yield in the field conditions. Plant Biotechnol. J. 9, 88\u201399.","journal-title":"Plant Biotechnol. J."},{"key":"664_CR34","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","volume":"29","author":"MW Pfaffl","year":"2001","unstructured":"Pfaffl M W. 2001 A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45.","journal-title":"Nucleic Acids Res."},{"key":"664_CR35","first-page":"95","volume":"198","author":"DG Robinson","year":"1996","unstructured":"Robinson D. G., Haschke H. P., Hinz G., Hoh B., Maeshima M. and Marty F. 1996 Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons. Planta 198, 95\u2013103.","journal-title":"Planta"},{"key":"664_CR36","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1438-8677.1998.tb00685.x","volume":"111","author":"DG Robinson","year":"1998","unstructured":"Robinson D. G., Hoppenrath M., Oberbeck K., Luykx P. and Ratajczak R. 1998 Localization of pyrophosphatase and V-ATPase in Chlamydomonas reinhardtii. Bot. Acta 111, 108\u2013122.","journal-title":"Bot. Acta"},{"key":"664_CR37","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1007\/BF00040721","volume":"31","author":"Y Sakakibara","year":"1996","unstructured":"Sakakibara Y., Kobayashi H. and Kasamo K. 1996 Isolation and characterization of cDNAs encoding vacuolar H +-pyrophosphatase isoforms from rice (Oryza sativa L.) Plant Mol. Biol. 31, 1029\u20131038.","journal-title":"Plant Mol. Biol."},{"key":"664_CR38","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1073\/pnas.89.5.1775","volume":"89","author":"V Sarafian","year":"1992","unstructured":"Sarafian V., Kim Y., Poole R. J. and Rea P. A. 1992 Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 89, 1775\u20131779.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"664_CR39","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","volume":"30","author":"K Tamura","year":"2013","unstructured":"Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S. 2013 MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725\u20132729.","journal-title":"Mol. Biol. Evol."},{"key":"664_CR40","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1006\/bbrc.1993.1164","volume":"190","author":"Y Tanaka","year":"1993","unstructured":"Tanaka Y., Chiba K., Maeda M. and Maeshima M. 1993 Molecular cloning of cDNA for vacuolar membrane proton-translocating inorganic pyrophosphatase in Hordeum vulgare. Biochem. Biophys. Res. Commun. 190, 1110\u20131114.","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"664_CR41","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1104\/pp.104.4.1131","volume":"104","author":"S Yoshida","year":"1994","unstructured":"Yoshida S. 1994 Low temperature-induced cytoplasmic acidosis in cultured mung bean (Vigna radiata L.) cells. Plant Physiol. 104, 1131\u20131138.","journal-title":"Plant Physiol."},{"key":"664_CR42","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jplph.2004.03.010","volume":"162","author":"C Z\u00f6rb","year":"2005","unstructured":"Z\u00f6rb C., Noll A., Karl S., Leib K., Yan F. and Schubert S. 2005 Molecular characterization of Na +\/H + antiporters (ZmNHX) of maize (Zea mays L.) and their expression under salt stress . J. Plant Physiol. 162, 55\u201366.","journal-title":"J. Plant Physiol."}],"container-title":["Journal of Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12041-016-0664-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12041-016-0664-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12041-016-0664-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,24]],"date-time":"2017-06-24T18:32:31Z","timestamp":1498329151000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12041-016-0664-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,16]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["664"],"URL":"https:\/\/doi.org\/10.1007\/s12041-016-0664-8","relation":{},"ISSN":["0022-1333","0973-7731"],"issn-type":[{"value":"0022-1333","type":"print"},{"value":"0973-7731","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,16]]}}}