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The precise effect of miR-488-5p in HSCs during hepatic fibrosis has not been elucidated.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In our study, qRT\u2010PCR was applied to assess the level of miR-488-5p in activated HSCs stimulated by TGF-\u03b21. We built murine liver fibrosis models with carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>), high-fat diet (HFD) and bile duct ligation (BDL). In vitro, the effects of miR-488-5p in HSCs were examined through cell proliferation assay and apoptosis. Luciferase reporter assay was applied to identify the underlying target of miR-488-5p. In vivo, the effects of miR-488-5p were explored through mouse liver fibrosis models.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The reduction of miR-488-5p in the activated HSCs induced by TGF-\u03b21 and three mouse hepatic fibrosis models were identified. The in vitro functional experimentations verified that miR-488-5p restrained expression of fibrosis-related markers and proliferative capacity in HSCs. Mechanically, we identified that miR-488-5p inhibited tet methylcytosine dioxygenase 3 (TET3) expression via straightly binding onto the 3\u2032 UTR of its mRNA, which sequentially restrained the TGF-\u03b2\/Smad2\/3 pathway. TET3 inhibition induced by the overexpression of miR-488-5p reduced extracellular matrix deposition, which contributed to mitigating mouse liver fibrosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>We highlight that miR-488-5p restrains the activation of HSCs and hepatic fibrosis via targeting TET3 which is involved in the TGF-\u03b2\/Smad2\/3 signaling pathway. Collectively, miR-488-5p is identified as a potential therapeutic target for hepatic fibrosis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s12072-022-10404-w","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T11:06:10Z","timestamp":1661339170000},"page":"463-475","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["miR-488-5p mitigates hepatic stellate cell activation and hepatic fibrosis via suppressing TET3 expression"],"prefix":"10.1007","volume":"17","author":[{"given":"Jiannan","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shasha","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunping","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,8,24]]},"reference":[{"issue":"4","key":"10404_CR1","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1136\/gut.46.4.443","volume":"46","author":"RC Benyon","year":"2000","unstructured":"Benyon RC, Iredale JP. 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