{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T12:47:10Z","timestamp":1770468430456,"version":"3.49.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270626"],"award-info":[{"award-number":["82270626"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"China Mega-Project for Infectious Diseases","award":["2013ZX10002005"],"award-info":[{"award-number":["2013ZX10002005"]}]},{"name":"China Mega-Project for Infectious Diseases","award":["2017ZX10203202"],"award-info":[{"award-number":["2017ZX10203202"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Hepatol Int"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Evaluation of liver fibrosis played a monumental role in the diagnosis and monitoring of chronic hepatitis B (CHB). We aimed to explore the value of serum<jats:italic> N<\/jats:italic>-glycan markers in liver fibrosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>This multi-center (33 hospitals) study recruited 760 treatment-na\u00efve CHB patients who underwent liver biopsy. Serum <jats:italic>N<\/jats:italic>-glycan markers were analyzed by DNA sequencer-assisted fluorophore-assisted with capillary electrophoresis (DSA-FACE) technology. First, we explore the relationship between 12 serum <jats:italic>N<\/jats:italic>-glycan markers and the fibrosis stage. Then, we developed a Px score for diagnosing significant fibrosis using the LASSO regression. Next, we compared the diagnostic performances between Px, LSM, APRI, and FIB-4. Finally, we explored the relationships between glycosyltransferase gene and liver fibrosis with RNA-transcriptome sequencing.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We included 622 CHB participants: male-dominated (69.6%); median age 42.0 (IQR 34.0\u201350.0); 287 with normal ALT; 73.0% with significant fibrosis. P5(NA2), P8(NA3), and P10(NA4) were opposite to the degree of fibrosis, while other profiles (except for P0[NGA2]) increased with the degree of fibrosis. Seven profiles (P1[NGA2F], P2[NGA2FB], P3[NG1A2F], P4[NG1A2F], P7[NA2FB], P8[NA3], and P9[NA3Fb]) were selected into Px score. Px score was associated with an increased risk of significant fibrosis (for per Px score increase, the risk of significant fibrosis was increased by 3.54 times (OR\u2009=\u20094.54 [2.63\u20137.82]) in the fully-adjusted generalized linear model. <jats:italic>p<\/jats:italic> for trend was &lt;0.001. The diagnostic performance of the Px score was superior to others. Glycosyltransferase genes were overexpressed in liver fibrosis, and glycosylation and glycosyltransferase-related pathways were significantly enriched.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Serum <jats:italic>N<\/jats:italic>-glycan markers were positively correlated with liver fibrosis. Px score had good performance in distinguishing significant fibrosis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s12072-024-10709-y","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T08:01:56Z","timestamp":1721203316000},"page":"1434-1447","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dose\u2013response relationship between serum N-glycan markers and liver fibrosis in chronic hepatitis B"],"prefix":"10.1007","volume":"18","author":[{"given":"Chi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiqiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8069-9901","authenticated-orcid":false,"given":"Hong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"China HepB-Related Fibrosis Assessment Research Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Hua","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhan-Qing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Feng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Hua","family":"Shang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Zhi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An-Lin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Lian","family":"Gui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Xia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Shang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-Bin","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu-Qing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Qiang","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Ping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Xiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu-Chun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Hua","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Bin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Hua","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Bin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Qiang","family":"Mi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-Zhong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Jin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Ming","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan-Ping","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Hua","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lang","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Lan","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian-Zhi","family":"Lou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Feng","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,17]]},"reference":[{"key":"10709_CR1","unstructured":"Global prevalence, cascade of care, and prophylaxis coverage of hepatitis B in 2022: a modelling study. Lancet Gastroenterol Hepatol 2023;8:879\u2013907"},{"issue":"67","key":"10709_CR2","first-page":"370","volume":"2017","author":"EASL","year":"2017","unstructured":"EASL. Clinical practice guidelines on the management of hepatitis B virus infection. J Hepatol 2017;2017(67):370\u2013398","journal-title":"J Hepatol"},{"key":"10709_CR3","doi-asserted-by":"publisher","first-page":"1560","DOI":"10.1002\/hep.29800","volume":"67","author":"NA Terrault","year":"2018","unstructured":"Terrault NA, Lok ASF, McMahon BJ, Chang KM, Hwang JP, Jonas MM, et al. Update on prevention, diagnosis, and treatment of chronic hepatitis B: AASLD 2018 hepatitis B guidance. Hepatology. 2018;67:1560\u20131599","journal-title":"Hepatology"},{"key":"10709_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12072-015-9675-4","volume":"10","author":"SK Sarin","year":"2016","unstructured":"Sarin SK, Kumar M, Lau GK, Abbas Z, Chan HL, Chen CJ, et al. Asian-Pacific clinical practice guidelines on the management of hepatitis B: a 2015 update. Hepatol Int 2016;10:1\u201398","journal-title":"Hepatol Int"},{"issue":"22","key":"10709_CR5","first-page":"29","volume":"2023","author":"The Chinese Chapter of International Hepato-Pancreato-Biliary Association","year":"2023","unstructured":"The Chinese Chapter of International Hepato-Pancreato-Biliary Association, Chinese Society of Liver Cancer, Society for Hepato-pancreato-biliary Surgery of Chinese Research Hospital Association SfVaToCRHA. Chinese expert consensus on antiviral therapy for hepatitis B virus-related hepatocellular carcinoma (2023 edition) (in Chinese). Chin J Dig Surg 2023;2023(22):29\u201341","journal-title":"Chin J Dig Surg"},{"key":"10709_CR6","unstructured":"Clinical Registration Survey of Primary Liver Cancer in China (CLCS) Mid 2020 Report (in Chinese). https:\/\/documents.pub\/document\/clcs-2020.html?page=1. Accessed 21 Jan 2024"},{"key":"10709_CR7","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1016\/S0140-6736(12)61425-1","volume":"381","author":"P Marcellin","year":"2013","unstructured":"Marcellin P, Gane E, Buti M, Afdhal N, Sievert W, Jacobson IM, et al. Regression of cirrhosis during treatment with tenofovir disoproxil fumarate for chronic hepatitis B: a 5-year open-label follow-up study. Lancet 2013;381:468\u2013475","journal-title":"Lancet"},{"key":"10709_CR8","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1056\/NEJMra1610570","volume":"377","author":"EB Tapper","year":"2017","unstructured":"Tapper EB, Lok AS. Use of liver imaging and biopsy in clinical practice. N Engl J Med 2017;377:756\u2013768","journal-title":"N Engl J Med"},{"key":"10709_CR9","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1136\/bmj.315.7106.453","volume":"315","author":"GR Foster","year":"1997","unstructured":"Foster GR, Goldin RD, Main J, Murray-Lyon I, Hargreaves S, Thomas HC. Management of chronic hepatitis C: clinical audit of biopsy based management algorithm. BMJ 1997;315:453\u2013458","journal-title":"BMJ"},{"key":"10709_CR10","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1016\/S0140-6736(23)01174-1","volume":"402","author":"M Serra-Burriel","year":"2023","unstructured":"Serra-Burriel M, Juanola A, Serra-Burriel F, Thiele M, Graupera I, Pose E, et al. Development, validation, and prognostic evaluation of a risk score for long-term liver-related outcomes in the general population: a multicohort study. Lancet 2023;402:988\u2013996","journal-title":"Lancet"},{"key":"10709_CR11","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1038\/nrm4073","volume":"16","author":"C Xu","year":"2015","unstructured":"Xu C, Ng DT. Glycosylation-directed quality control of protein folding. Nat Rev Mol Cell Biol 2015;16:742\u2013752","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10709_CR12","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1038\/nrm3383","volume":"13","author":"KW Moremen","year":"2012","unstructured":"Moremen KW, Tiemeyer M, Nairn AV. Vertebrate protein glycosylation: diversity, synthesis and function. Nat Rev Mol Cell Biol 2012;13:448\u2013462","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10709_CR13","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.1002\/hep.21855","volume":"46","author":"XE Liu","year":"2007","unstructured":"Liu XE, Desmyter L, Gao CF, Laroy W, Dewaele S, Vanhooren V, et al. N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus. Hepatology 2007;46:1426\u20131435","journal-title":"Hepatology"},{"key":"10709_CR14","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1111\/apt.17748","volume":"59","author":"E Butaye","year":"2024","unstructured":"Butaye E, Somers N, Grossar L, Pauwels N, Lefere S, Devisscher L, et al. Systematic review: glycomics as diagnostic markers for hepatocellular carcinoma. Aliment Pharmacol Ther 2024;59:23\u201338","journal-title":"Aliment Pharmacol Ther"},{"key":"10709_CR15","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.eng.2023.03.008","volume":"26","author":"L Wang","year":"2023","unstructured":"Wang L, Liu Y, Gu Q, Zhang C, Xu L, Wang L, et al. Serum N-glycan markers for diagnosing significant liver fibrosis and cirrhosis in chronic hepatitis B patients with normal alanine aminotransferase levels. Engineering 2023;26:151\u2013158","journal-title":"Engineering"},{"key":"10709_CR16","doi-asserted-by":"publisher","first-page":"3911","DOI":"10.1002\/jmv.27779","volume":"94","author":"C Zhang","year":"2022","unstructured":"Zhang C, Liu Y, Li J, Liu H, Shao C, Liu D, et al. Dose-response relationship between qAnti-HBc and liver inflammation in chronic hepatitis B with normal or mildly elevated alanine transaminase based on liver biopsy. J Med Virol 2022;94:3911\u20133923","journal-title":"J Med Virol"},{"key":"10709_CR17","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1016\/j.jhep.2007.07.006","volume":"47","author":"ZD Goodman","year":"2007","unstructured":"Goodman ZD. Grading and staging systems for inflammation and fibrosis in chronic liver diseases. J Hepatol 2007;47:598\u2013607","journal-title":"J Hepatol"},{"key":"10709_CR18","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.1016\/S0140-6736(07)61602-X","volume":"370","author":"E von Elm","year":"2007","unstructured":"von Elm E, Altman DG, Egger M, Pocock SJ, G\u00f8tzsche PC, Vandenbroucke JP. The strengthening the reporting of observational studies in epidemiology (STROBE) statement: guidelines for reporting observational studies. Lancet 2007;370:1453\u20131457","journal-title":"Lancet"},{"key":"10709_CR19","unstructured":"https:\/\/www.cn-healthcare.com\/articlewm\/20230620\/content-1567760.html (2023). Accessed 3 Feb 2024"},{"key":"10709_CR20","doi-asserted-by":"publisher","first-page":"344","DOI":"10.21037\/jgo-22-93","volume":"13","author":"L Guo","year":"2022","unstructured":"Guo L, Wan L, Hu Y, Huang H, He B, Wen Z. Serum N-glycan profiling as a diagnostic biomarker for the identification of hepatitis B virus-associated hepatocellular carcinoma. J Gastrointest Oncol 2022;13:344\u2013354","journal-title":"J Gastrointest Oncol"},{"key":"10709_CR21","doi-asserted-by":"publisher","first-page":"10231","DOI":"10.1021\/acs.analchem.3c00533","volume":"95","author":"J Wang","year":"2023","unstructured":"Wang J, Liu J, Li M, Wang Y, Man Q, Zhang H, et al. Novel three-dimensional hierarchical porous carbon probe for the discovery of N-glycan biomarkers and early hepatocellular carcinoma detection. Anal Chem 2023;95:10231\u201310240","journal-title":"Anal Chem"},{"key":"10709_CR22","first-page":"1336","volume":"37","author":"X Cao","year":"2021","unstructured":"Cao X, Sun Y, Chen C, Xiao Y, Xiang K, Liu X, et al. Association between the alteration of serum N-glycan profile and the change of glycosyltransferase expression in liver tissue in patients with hepatitis B virus\u2014related hepatocellular carcinoma (in Chinese). J Clin Hepatol 2021;37:1336\u20131341","journal-title":"J Clin Hepatol"},{"key":"10709_CR23","unstructured":"Chinese Society of Hepatology CMA, Chinese Society of Infectious Diseases CMA. Guidelines for the prevention and treatment of chronic hepatitis B (version 2022). Zhonghua Gan Zang Bing Za Zhi 2022;30:1309\u20131331"},{"key":"10709_CR24","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1111\/jgh.14498","volume":"34","author":"XQ Dong","year":"2019","unstructured":"Dong XQ, Wu Z, Li J, Wang GQ, Zhao H. Declining in liver stiffness cannot indicate fibrosis regression in patients with chronic hepatitis B: a 78-week prospective study. J Gastroenterol Hepatol 2019;34:755\u2013763","journal-title":"J Gastroenterol Hepatol"},{"key":"10709_CR25","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.14309\/ajg.0000000000001239","volume":"116","author":"D Ji","year":"2021","unstructured":"Ji D, Chen Y, Shang Q, Liu H, Tan L, Wang J, et al. Unreliable estimation of fibrosis regression during treatment by liver stiffness measurement in patients with chronic hepatitis B. Am J Gastroenterol 2021;116:1676\u20131685","journal-title":"Am J Gastroenterol"},{"key":"10709_CR26","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1016\/j.jhep.2021.05.025","volume":"75","author":"Liver EAftSot","year":"2021","unstructured":"Liver EAftSot. EASL clinical practice guidelines on non-invasive tests for evaluation of liver disease severity and prognosis\u20142021 update. J Hepatol 2021;75:659\u2013689","journal-title":"J Hepatol"},{"key":"10709_CR27","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1007\/s00535-021-01782-3","volume":"56","author":"J Itakura","year":"2021","unstructured":"Itakura J, Kurosaki M, Setoyama H, Simakami T, Oza N, Korenaga M, et al. Applicability of APRI and FIB-4 as a transition indicator of liver fibrosis in patients with chronic viral hepatitis. J Gastroenterol 2021;56:470\u2013478","journal-title":"J Gastroenterol"},{"key":"10709_CR28","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1016\/j.jhep.2019.01.037","volume":"70","author":"H Aberra","year":"2019","unstructured":"Aberra H, Desalegn H, Berhe N, Mekasha B, Medhin G, Gundersen SG, et al. The WHO guidelines for chronic hepatitis B fail to detect half of the patients in need of treatment in Ethiopia. J Hepatol 2019;70:1065\u20131071","journal-title":"J Hepatol"},{"key":"10709_CR29","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1038\/s41575-020-0296-6","volume":"17","author":"PA Revill","year":"2020","unstructured":"Revill PA, Tu T, Netter HJ, Yuen LKW, Locarnini SA, Littlejohn M. The evolution and clinical impact of hepatitis B virus genome diversity. Nat Rev Gastroenterol Hepatol 2020;17:618\u2013634","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"10709_CR30","doi-asserted-by":"publisher","first-page":"121675","DOI":"10.1016\/j.carbpol.2023.121675","volume":"327","author":"X Wang","year":"2024","unstructured":"Wang X, Liu J, Wang S, Xie Y, Liu Y, Fan J, et al. Online LC-ESI-MS\/MS comparative analysis of N\/O-glycopatterns in human colostrum from different ethnic groups in Northwest China. Carbohyd Polym 2024;327:121675","journal-title":"Carbohyd Polym"}],"container-title":["Hepatology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12072-024-10709-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12072-024-10709-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12072-024-10709-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T19:08:01Z","timestamp":1728414481000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12072-024-10709-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,17]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10709"],"URL":"https:\/\/doi.org\/10.1007\/s12072-024-10709-y","relation":{},"ISSN":["1936-0533","1936-0541"],"issn-type":[{"value":"1936-0533","type":"print"},{"value":"1936-0541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,17]]},"assertion":[{"value":"9 April 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 June 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Cuiying Chen, Junli Zhang, and Chao Zhang are from Sysdiagno (Nanjing) Biotechnology Company Limited. Chi Zhang, Yiqi Liu, Lin Wang, Xueen Liu, Guiqiang Wang, Hui Zhuang, and Hong Zhao declare that they have no conflicts of interest or financial conflicts to disclose.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was approved by The Ethical Committees of Peking University First Hospital and participating hospitals. All patients signed informed consent before enrollment.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}