{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:19:31Z","timestamp":1781659171854,"version":"3.54.5"},"reference-count":73,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20232ACB206053"],"award-info":[{"award-number":["20232ACB206053"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013064","name":"Key Research and Development Program of Jiangxi Province","doi-asserted-by":"publisher","award":["20203BBCL73205"],"award-info":[{"award-number":["20203BBCL73205"]}],"id":[{"id":"10.13039\/501100013064","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Transl Oncol"],"DOI":"10.1007\/s12094-025-04150-2","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T05:59:44Z","timestamp":1764568784000},"page":"1464-1475","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["CTC cluster in breast cancer: synergetic metastasis promotion mechanism and transformation path from platelet cloaking to leukocyte escort"],"prefix":"10.1007","volume":"28","author":[{"given":"Cangtai","family":"Guan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangyu","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyao","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9463-6908","authenticated-orcid":false,"given":"Lixiang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"4150_CR1","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1038\/s41591-024-03486-6","volume":"31","author":"C Kurzeder","year":"2025","unstructured":"Kurzeder C, Nguyen-Str\u00e4uli BD, Krol I, Ring A, Castro-Giner F, N\u00fcesch M, et al. Digoxin for reduction of circulating tumor cell cluster size in metastatic breast cancer: a proof-of-concept trial. Nat Med. 2025;31:1120\u20134.","journal-title":"Nat Med"},{"key":"4150_CR2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-025-01733-y","volume":"18","author":"C Yang","year":"2025","unstructured":"Yang C, Liu C, Xia C, Fu L. Clinical applications of circulating tumor cells in metastasis and therapy. J Hematol Oncol. 2025;18:80.","journal-title":"J Hematol Oncol"},{"key":"4150_CR3","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-62862-z","volume":"16","author":"E Schuster","year":"2025","unstructured":"Schuster E, Dashzeveg NK, Tong F, Jia Y, El-Shennawy L, Zhang T, et al. Computational ranking identifies Plexin-B2 in circulating tumor cell clustering with monocytes in breast cancer metastasis. Nat Commun. 2025;16:7649.","journal-title":"Nat Commun"},{"key":"4150_CR4","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1038\/s41588-025-02205-2","volume":"57","author":"D Gremmelspacher","year":"2025","unstructured":"Gremmelspacher D, Gawron J, Szczerba BM, Jahn K, Castro-Giner F, Kuipers J, et al. Phylogenetic inference reveals clonal heterogeneity in circulating tumor cell clusters. Nat Genet. 2025;57:1357\u201361.","journal-title":"Nat Genet"},{"key":"4150_CR5","volume":"1877","author":"K Morris","year":"2022","unstructured":"Morris K, Schnoor B, Papa AL. Platelet cancer cell interplay as a new therapeutic target. Biochimica et Biophysica Acta (BBA). 2022;1877:188770.","journal-title":"Biochimica et Biophysica Acta (BBA)"},{"key":"4150_CR6","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1038\/s41392-024-01938-6","volume":"9","author":"X Gu","year":"2024","unstructured":"Gu X, Wei S, Lv X. Circulating tumor cells: from new biological insights to clinical practice. Signal Transduct Target Ther. 2024;9:226.","journal-title":"Signal Transduct Target Ther"},{"key":"4150_CR7","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1016\/j.cell.2014.07.013","volume":"158","author":"N Aceto","year":"2014","unstructured":"Aceto N, Bardia A, Miyamoto DT, Donaldson MC, Wittner BS, Spencer JA, et al. Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 2014;158:1110\u201322.","journal-title":"Cell"},{"key":"4150_CR8","doi-asserted-by":"publisher","first-page":"E854","DOI":"10.1073\/pnas.1508541113","volume":"113","author":"KJ Cheung","year":"2016","unstructured":"Cheung KJ, Padmanaban V, Silvestri V, Schipper K, Cohen JD, Fairchild AN, et al. Polyclonal breast cancer metastases arise from collective dissemination of keratin 14-expressing tumor cell clusters. Proc Natl Acad Sci U S A. 2016;113:E854-863.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"4150_CR9","doi-asserted-by":"publisher","first-page":"2800","DOI":"10.7150\/jca.31411","volume":"10","author":"L Huang","year":"2019","unstructured":"Huang L, Ji H, Yin L, Niu X, Wang Y, Liu Y, et al. High expression of plakoglobin promotes metastasis in invasive micropapillary carcinoma of the breast via tumor cluster formation. J Cancer. 2019;10:2800\u201310.","journal-title":"J Cancer"},{"key":"4150_CR10","doi-asserted-by":"publisher","first-page":"10904","DOI":"10.1074\/jbc.271.18.10904","volume":"271","author":"LL Witcher","year":"1996","unstructured":"Witcher LL, Collins R, Puttagunta S, Mechanic SE, Munson M, Gumbiner B, et al. Desmosomal cadherin binding domains of plakoglobin. J Biol Chem. 1996;271:10904\u20139.","journal-title":"J Biol Chem"},{"key":"4150_CR11","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/s40364-017-0099-2","volume":"5","author":"W Goto","year":"2017","unstructured":"Goto W, Kashiwagi S, Asano Y, Takada K, Takahashi K, Hatano T, et al. Circulating tumor cell clusters-associated gene plakoglobin is a significant prognostic predictor in patients with breast cancer. Biomark Res. 2017;5:19.","journal-title":"Biomark Res"},{"key":"4150_CR12","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1182\/blood-2004-06-2272","volume":"105","author":"JS Palumbo","year":"2005","unstructured":"Palumbo JS, Talmage KE, Massari JV, La Jeunesse CM, Flick MJ, Kombrinck KW, et al. Platelets and fibrin(ogen) increase metastatic potential by impeding natural killer cell-mediated elimination of tumor cells. Blood. 2005;105:178\u201385.","journal-title":"Blood"},{"key":"4150_CR13","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1007\/s12253-012-9531-y","volume":"18","author":"L Gong","year":"2012","unstructured":"Gong L, Cai Y, Zhou X, Yang H. Activated platelets interact with lung cancer cells through P-selectin glycoprotein ligand-1. Pathol Oncol Res. 2012;18:989\u201396.","journal-title":"Pathol Oncol Res"},{"key":"4150_CR14","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.1182\/blood.V96.5.1789","volume":"96","author":"OJ McCarty","year":"2000","unstructured":"McCarty OJ, Mousa SA, Bray PF, Konstantopoulos K. Immobilized platelets support human colon carcinoma cell tethering, rolling, and firm adhesion under dynamic flow conditions. Blood. 2000;96:1789\u201397.","journal-title":"Blood"},{"key":"4150_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/cancers9100133","author":"M Lavergne","year":"2017","unstructured":"Lavergne M, Janus-Bell E, Schaff M, Gachet C, Mangin PH. Platelet integrins in tumor metastasis: do they represent a therapeutic target? Cancers (Basel). 2017. https:\/\/doi.org\/10.3390\/cancers9100133.","journal-title":"Cancers (Basel)"},{"key":"4150_CR16","doi-asserted-by":"publisher","first-page":"2168","DOI":"10.1074\/jbc.M111.269811","volume":"287","author":"AS Lonsdorf","year":"2012","unstructured":"Lonsdorf AS, Kr\u00e4mer BF, Fahrleitner M, Sch\u00f6nberger T, Gnerlich S, Ring S, et al. Engagement of \u03b1IIb\u03b23 (GPIIb\/IIIa) with \u03b1\u03bd\u03b23 integrin mediates interaction of melanoma cells with platelets: a connection to hematogenous metastasis. J Biol Chem. 2012;287:2168\u201378.","journal-title":"J Biol Chem"},{"key":"4150_CR17","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1016\/j.ccr.2011.09.009","volume":"20","author":"M Labelle","year":"2011","unstructured":"Labelle M, Begum S, Hynes RO. Direct signaling between platelets and cancer cells induces an epithelial-mesenchymal-like transition and promotes metastasis. Cancer Cell. 2011;20:576\u201390.","journal-title":"Cancer Cell"},{"key":"4150_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01347-1","volume":"20","author":"MP Ward","year":"2021","unstructured":"Ward MP, L EK, L AN, Mohamed BM, Kelly T, Bates M, et al. Platelets, immune cells and the coagulation cascade; friend or foe of the circulating tumour cell? Mol Cancer. 2021;20:59.","journal-title":"Mol Cancer"},{"key":"4150_CR19","doi-asserted-by":"publisher","first-page":"3498","DOI":"10.1039\/C7LC00654C","volume":"17","author":"X Jiang","year":"2017","unstructured":"Jiang X, Wong KHK, Khankhel AH, Zeinali M, Reategui E, Phillips MJ, et al. Microfluidic isolation of platelet-covered circulating tumor cells. Lab Chip. 2017;17:3498\u2013503.","journal-title":"Lab Chip"},{"key":"4150_CR20","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13112723","author":"YP Yang","year":"2021","unstructured":"Yang YP, Giret TM, Cote RJ. Circulating tumor cells from enumeration to analysis: current challenges and future opportunities. Cancers (Basel). 2021. https:\/\/doi.org\/10.3390\/cancers13112723.","journal-title":"Cancers (Basel)"},{"key":"4150_CR21","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1038\/s41586-019-0915-y","volume":"566","author":"BM Szczerba","year":"2019","unstructured":"Szczerba BM, Castro-Giner F, Vetter M, Krol I, Gkountela S, Landin J, et al. Neutrophils escort circulating tumour cells to enable cell cycle progression. Nature. 2019;566:553\u20137.","journal-title":"Nature"},{"key":"4150_CR22","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.devcel.2019.04.021","volume":"49","author":"O Iriondo","year":"2019","unstructured":"Iriondo O, Yu M. Unexpected friendship: neutrophils help tumor cells en route to metastasis. Dev Cell. 2019;49:308\u201310.","journal-title":"Dev Cell"},{"key":"4150_CR23","doi-asserted-by":"publisher","first-page":"1099921","DOI":"10.3389\/fimmu.2023.1099921","volume":"14","author":"JM Rozenberg","year":"2023","unstructured":"Rozenberg JM, Buzdin AA, Mohammad T, Rakitina OA, Didych DA, Pleshkan VV, et al. Molecules promoting circulating clusters of cancer cells suggest novel therapeutic targets for treatment of metastatic cancers. Front Immunol. 2023;14:1099921.","journal-title":"Front Immunol"},{"key":"4150_CR24","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.937565","volume":"13","author":"J Zhao","year":"2022","unstructured":"Zhao J, Jin J. Neutrophil extracellular traps: new players in cancer research. Front Immunol. 2022;13:937565.","journal-title":"Front Immunol"},{"key":"4150_CR25","doi-asserted-by":"publisher","first-page":"3446","DOI":"10.1172\/JCI67484","volume":"123","author":"J Cools-Lartigue","year":"2013","unstructured":"Cools-Lartigue J, Spicer J, McDonald B, Gowing S, Chow S, Giannias B, et al. Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis. J Clin Invest. 2013;123:3446\u201358.","journal-title":"J Clin Invest"},{"key":"4150_CR26","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1016\/j.it.2019.04.009","volume":"40","author":"B Guo","year":"2019","unstructured":"Guo B, Oliver TG. Partners in crime: neutrophil-CTC collusion in metastasis. Trends Immunol. 2019;40:556\u20139.","journal-title":"Trends Immunol"},{"key":"4150_CR27","doi-asserted-by":"publisher","first-page":"3427","DOI":"10.1182\/blood-2009-10-247296","volume":"115","author":"L Erpenbeck","year":"2010","unstructured":"Erpenbeck L, Sch\u00f6n MP. Deadly allies: the fatal interplay between platelets and metastasizing cancer cells. Blood. 2010;115:3427\u201336.","journal-title":"Blood"},{"key":"4150_CR28","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1007\/s10585-014-9660-7","volume":"31","author":"K Egan","year":"2014","unstructured":"Egan K, Cooke N, Kenny D. Living in shear: platelets protect cancer cells from shear induced damage. Clin Exp Metastasis. 2014;31:697\u2013704.","journal-title":"Clin Exp Metastasis"},{"key":"4150_CR29","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1186\/s40001-023-01342-w","volume":"28","author":"L Zhou","year":"2023","unstructured":"Zhou L, Zhang Z, Tian Y, Li Z, Liu Z, Zhu S. The critical role of platelet in cancer progression and metastasis. Eur J Med Res. 2023;28:385.","journal-title":"Eur J Med Res"},{"key":"4150_CR30","doi-asserted-by":"publisher","first-page":"15477","DOI":"10.1038\/s41598-021-94735-y","volume":"11","author":"S Anvari","year":"2021","unstructured":"Anvari S, Osei E, Maftoon N. Interactions of platelets with circulating tumor cells contribute to cancer metastasis. Sci Rep. 2021;11:15477.","journal-title":"Sci Rep"},{"key":"4150_CR31","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.613170","volume":"11","author":"K Pyrillou","year":"2020","unstructured":"Pyrillou K, Burzynski LC, Clarke MCH. Alternative pathways of IL-1 activation, and its role in health and disease. Front Immunol. 2020;11:613170.","journal-title":"Front Immunol"},{"key":"4150_CR32","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1016\/S0195-668X(03)00239-2","volume":"24","author":"V Rold\u00e1n","year":"2003","unstructured":"Rold\u00e1n V, Mar\u00edn F, Blann AD, Garc\u00eda A, Marco P, Sogorb F, et al. Interleukin-6, endothelial activation and thrombogenesis in chronic atrial fibrillation. Eur Heart J. 2003;24:1373\u201380.","journal-title":"Eur Heart J"},{"key":"4150_CR33","doi-asserted-by":"publisher","first-page":"4947","DOI":"10.1073\/pnas.1524448113","volume":"113","author":"SH Au","year":"2016","unstructured":"Au SH, Storey BD, Moore JC, Tang Q, Chen YL, Javaid S, et al. Clusters of circulating tumor cells traverse capillary-sized vessels. Proc Natl Acad Sci U S A. 2016;113:4947\u201352.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"4150_CR34","doi-asserted-by":"publisher","first-page":"23231","DOI":"10.1038\/s41598-021-02482-x","volume":"11","author":"C Casas-Arozamena","year":"2021","unstructured":"Casas-Arozamena C, Otero-Cacho A, Carnero B, Almenglo C, Aymerich M, Alonso-Alconada L, et al. Haemodynamic-dependent arrest of circulating tumour cells at large blood vessel bifurcations as new model for metastasis. Sci Rep. 2021;11:23231.","journal-title":"Sci Rep"},{"key":"4150_CR35","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1182\/blood.V38.1.39.39","volume":"38","author":"HE Kattlove","year":"1971","unstructured":"Kattlove HE, Alexander B. The effect of cold on platelets. I. Cold-induced platelet aggregation. Blood. 1971;38:39\u201348.","journal-title":"Blood"},{"key":"4150_CR36","doi-asserted-by":"publisher","first-page":"1607","DOI":"10.1161\/ATVBAHA.111.226373","volume":"31","author":"A Straub","year":"2011","unstructured":"Straub A, Krajewski S, Hohmann JD, Westein E, Jia F, Bassler N, et al. Evidence of platelet activation at medically used hypothermia and mechanistic data indicating ADP as a key mediator and therapeutic target. Arterioscler Thromb Vasc Biol. 2011;31:1607\u201316.","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"4150_CR37","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1093\/clinchem\/48.6.891","volume":"48","author":"M Macey","year":"2002","unstructured":"Macey M, Azam U, McCarthy D, Webb L, Chapman ES, Okrongly D, et al. Evaluation of the anticoagulants EDTA and citrate, theophylline, adenosine, and dipyridamole (CTAD) for assessing platelet activation on the ADVIA 120 hematology system. Clin Chem. 2002;48:891\u20139.","journal-title":"Clin Chem"},{"key":"4150_CR38","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1002\/cyto.b.10001","volume":"51","author":"M Macey","year":"2003","unstructured":"Macey M, McCarthy D, Azam U, Milne T, Golledge P, Newland A. Ethylenediaminetetraacetic acid plus citrate-theophylline-adenosine-dipyridamole (EDTA-CTAD): a novel anticoagulant for the flow cytometric assessment of platelet and neutrophil activation ex vivo in whole blood. Cytometry B Clin Cytom. 2003;51:30\u201340.","journal-title":"Cytometry B Clin Cytom"},{"key":"4150_CR39","doi-asserted-by":"publisher","DOI":"10.3390\/cells9081915","author":"M Mussbacher","year":"2020","unstructured":"Mussbacher M, Krammer TL, Heber S, Schrottmaier WC, Zeibig S, Holthoff HP, et al. Impact of anticoagulation and sample processing on the quantification of human blood-derived microRNA signatures. Cells. 2020. https:\/\/doi.org\/10.3390\/cells9081915.","journal-title":"Cells"},{"key":"4150_CR40","doi-asserted-by":"publisher","first-page":"198","DOI":"10.5858\/arpa.2016-0483-OA","volume":"142","author":"M Rodr\u00edguez-Lee","year":"2018","unstructured":"Rodr\u00edguez-Lee M, Kolatkar A, McCormick M, Dago AD, Kendall J, Carlsson NA, et al. Effect of blood collection tube type and time to processing on the enumeration and high-content characterization of circulating tumor cells using the high-definition single-cell assay. Arch Pathol Lab Med. 2018;142:198\u2013207.","journal-title":"Arch Pathol Lab Med"},{"key":"4150_CR41","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/978-1-0716-3271-0_2","volume":"2679","author":"SH Au","year":"2023","unstructured":"Au SH. Circulating tumor cell cluster sorting by size and asymmetry. Methods Mol Biol. 2023;2679:15\u201323.","journal-title":"Methods Mol Biol"},{"key":"4150_CR42","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1126\/science.1228522","volume":"339","author":"M Yu","year":"2013","unstructured":"Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, et al. Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 2013;339:580\u20134.","journal-title":"Science"},{"key":"4150_CR43","doi-asserted-by":"publisher","first-page":"3385","DOI":"10.1038\/s41467-022-31009-9","volume":"13","author":"M Boya","year":"2022","unstructured":"Boya M, Ozkaya-Ahmadov T, Swain BE, Chu CH, Asmare N, Civelekoglu O, et al. High throughput, label-free isolation of circulating tumor cell clusters in meshed microwells. Nat Commun. 2022;13:3385.","journal-title":"Nat Commun"},{"key":"4150_CR44","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/nmeth.3404","volume":"12","author":"AF Sarioglu","year":"2015","unstructured":"Sarioglu AF, Aceto N, Kojic N, Donaldson MC, Zeinali M, Hamza B, et al. A microfluidic device for label-free, physical capture of circulating tumor cell clusters. Nat Methods. 2015;12:685\u201391.","journal-title":"Nat Methods"},{"key":"4150_CR45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0260124","volume":"16","author":"EP Darga","year":"2021","unstructured":"Darga EP, Dolce EM, Fang F, Kidwell KM, Gersch CL, Kregel S, et al. PD-L1 expression on circulating tumor cells and platelets in patients with metastatic breast cancer. PLoS ONE. 2021;16:e0260124.","journal-title":"PLoS ONE"},{"key":"4150_CR46","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051111","author":"C Costa","year":"2020","unstructured":"Costa C, Muinelo-Romay L, Cebey-L\u00f3pez V, Pereira-Veiga T, Mart\u00ednez-Pena I, Abreu M, et al. Analysis of a real-world cohort of metastatic breast cancer patients shows circulating tumor cell clusters (CTC-clusters) as predictors of patient outcomes. Cancers (Basel). 2020. https:\/\/doi.org\/10.3390\/cancers12051111.","journal-title":"Cancers (Basel)"},{"key":"4150_CR47","doi-asserted-by":"publisher","DOI":"10.1186\/s13058-018-0976-0","volume":"20","author":"AM Larsson","year":"2018","unstructured":"Larsson AM, Jansson S, Bendahl PO, Levin Tykjaer J\u00f6rgensen C, Loman N, Graffman C, et al. Longitudinal enumeration and cluster evaluation of circulating tumor cells improve prognostication for patients with newly diagnosed metastatic breast cancer in a prospective observational trial. Breast Cancer Res. 2018;20:48.","journal-title":"Breast Cancer Res"},{"key":"4150_CR48","doi-asserted-by":"publisher","first-page":"6089","DOI":"10.1158\/1078-0432.CCR-19-0208","volume":"25","author":"C Paoletti","year":"2019","unstructured":"Paoletti C, Miao J, Dolce EM, Darga EP, Repollet MI, Doyle GV, et al. Circulating tumor cell clusters in patients with metastatic breast cancer: a SWOG S0500 translational medicine study. Clin Cancer Res. 2019;25:6089\u201397.","journal-title":"Clin Cancer Res"},{"issue":"10","key":"4150_CR49","first-page":"2356","volume":"13","author":"C Reduzzi","year":"2021","unstructured":"Reduzzi C, Di Cosimo S, Gerratana L, Motta R, Martinetti A, Vingiani A, et al. Circulating tumor cell clusters are frequently detected in women with early-stage breast cancer. Cancers(Basel). 2021;13(10):2356.","journal-title":"Cancers(Basel)"},{"key":"4150_CR50","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1186\/1471-2407-12-178","volume":"12","author":"TM Gorges","year":"2012","unstructured":"Gorges TM, Tinhofer I, Drosch M, R\u00f6se L, Zollner TM, Krahn T, et al. Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition. BMC Cancer. 2012;12:178.","journal-title":"BMC Cancer"},{"key":"4150_CR51","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.canlet.2012.02.019","volume":"321","author":"K St\u00fcbke","year":"2012","unstructured":"St\u00fcbke K, Wicklein D, Herich L, Schumacher U, Nehmann N. Selectin-deficiency reduces the number of spontaneous metastases in a xenograft model of human breast cancer. Cancer Lett. 2012;321:89\u201399.","journal-title":"Cancer Lett"},{"key":"4150_CR52","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1093\/glycob\/cwu119","volume":"25","author":"AM Gomes","year":"2015","unstructured":"Gomes AM, Kozlowski EO, Borsig L, Teixeira FC, Vlodavsky I, Pav\u00e3o MS. Antitumor properties of a new non-anticoagulant heparin analog from the mollusk Nodipecten nodosus: effect on P-selectin, heparanase, metastasis and cellular recruitment. Glycobiology. 2015;25:386\u201393.","journal-title":"Glycobiology"},{"key":"4150_CR53","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.116108","volume":"171","author":"JM Motta","year":"2024","unstructured":"Motta JM, Micheli KVA, Roberto-Fernandes C, Hermsdorff-Brandt M, Guedes AL, Frattani FS, et al. A low-anticoagulant heparin suppresses metastatic dissemination through the inhibition of tumor cell-platelets association. Biomed Pharmacother. 2024;171:116108.","journal-title":"Biomed Pharmacother"},{"key":"4150_CR54","doi-asserted-by":"publisher","first-page":"17810","DOI":"10.18632\/oncotarget.24891","volume":"9","author":"M Smeda","year":"2018","unstructured":"Smeda M, Kieronska A, Proniewski B, Jasztal A, Selmi A, Wandzel K, et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget. 2018;9:17810\u201324.","journal-title":"Oncotarget"},{"key":"4150_CR55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230520","volume":"15","author":"M Smeda","year":"2020","unstructured":"Smeda M, Kij A, Proniewski B, Matyjaszczyk-Gwarda K, Przyborowski K, Jasztal A, et al. Unexpected effects of long-term treatment with acetylsalicylic acid on late phase of pulmonary metastasis in murine model of orthotopic breast cancer. PLoS ONE. 2020;15:e0230520.","journal-title":"PLoS ONE"},{"key":"4150_CR56","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1016\/j.ccr.2011.08.025","volume":"20","author":"Q Chen","year":"2011","unstructured":"Chen Q, Zhang XH, Massagu\u00e9 J. Macrophage binding to receptor VCAM-1 transmits survival signals in breast cancer cells that invade the lungs. Cancer Cell. 2011;20:538\u201349.","journal-title":"Cancer Cell"},{"key":"4150_CR57","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1016\/j.ccr.2011.11.002","volume":"20","author":"X Lu","year":"2011","unstructured":"Lu X, Mu E, Wei Y, Riethdorf S, Yang Q, Yuan M, et al. VCAM-1 promotes osteolytic expansion of indolent bone micrometastasis of breast cancer by engaging \u03b14\u03b21-positive osteoclast progenitors. Cancer Cell. 2011;20:701\u201314.","journal-title":"Cancer Cell"},{"key":"4150_CR58","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1038\/s41416-020-01227-3","volume":"124","author":"L Cabel","year":"2021","unstructured":"Cabel L, Berger F, Cottu P, Loirat D, Rampanou A, Brain E, et al. Clinical utility of circulating tumour cell-based monitoring of late-line chemotherapy for metastatic breast cancer: the randomised CirCe01 trial. Br J Cancer. 2021;124:1207\u201313.","journal-title":"Br J Cancer"},{"key":"4150_CR59","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1001\/jamaoncol.2020.5660","volume":"7","author":"FC Bidard","year":"2021","unstructured":"Bidard FC, Jacot W, Kiavue N, Dureau S, Kadi A, Brain E, et al. Efficacy of circulating tumor cell count-driven vs clinician-driven first-line therapy choice in hormone receptor-positive, ERBB2-negative metastatic breast cancer: the STIC CTC randomized clinical trial. JAMA Oncol. 2021;7:34\u201341.","journal-title":"JAMA Oncol"},{"key":"4150_CR60","doi-asserted-by":"publisher","DOI":"10.3390\/cells11081310","author":"A Cappenberg","year":"2022","unstructured":"Cappenberg A, Kardell M, Zarbock A. Selectin-mediated signaling-shedding light on the regulation of integrin activity in neutrophils. Cells. 2022. https:\/\/doi.org\/10.3390\/cells11081310.","journal-title":"Cells"},{"key":"4150_CR61","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1083\/jcb.136.3.717","volume":"136","author":"MB Lawrence","year":"1997","unstructured":"Lawrence MB, Kansas GS, Kunkel EJ, Ley K. Threshold levels of fluid shear promote leukocyte adhesion through selectins (CD62L,P,E). J Cell Biol. 1997;136:717\u201327.","journal-title":"J Cell Biol"},{"key":"4150_CR62","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0245536","volume":"16","author":"A Marrella","year":"2021","unstructured":"Marrella A, Fedi A, Varani G, Vaccari I, Fato M, Firpo G, et al. High blood flow shear stress values are associated with circulating tumor cells cluster disaggregation in a multi-channel microfluidic device. PLoS ONE. 2021;16:e0245536.","journal-title":"PLoS ONE"},{"key":"4150_CR63","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1038\/s41416-020-01176-x","volume":"124","author":"J Perea Paizal","year":"2021","unstructured":"Perea Paizal J, Au SH, Bakal C. Squeezing through the microcirculation: survival adaptations of circulating tumour cells to seed metastasis. Br J Cancer. 2021;124:58\u201365.","journal-title":"Br J Cancer"},{"key":"4150_CR64","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24087087","author":"B Zhang","year":"2023","unstructured":"Zhang B, Li X, Tang K, Xin Y, Hu G, Zheng Y, et al. Adhesion to the brain endothelium selects breast cancer cells with brain metastasis potential. Int J Mol Sci. 2023. https:\/\/doi.org\/10.3390\/ijms24087087.","journal-title":"Int J Mol Sci"},{"key":"4150_CR65","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13215272","author":"M Lim","year":"2021","unstructured":"Lim M, Park S, Jeong HO, Park SH, Kumar S, Jang A, et al. Circulating tumor cell clusters are cloaked with platelets and correlate with poor prognosis in unresectable pancreatic cancer. Cancers (Basel). 2021. https:\/\/doi.org\/10.3390\/cancers13215272.","journal-title":"Cancers (Basel)"},{"key":"4150_CR66","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1038\/s41523-024-00706-7","volume":"10","author":"T Thomas-Bonafos","year":"2024","unstructured":"Thomas-Bonafos T, Pierga JY, Bidard FC, Cabel L, Kiavue N. Circulating tumor cells in breast cancer: clinical validity and utility. NPJ Breast Cancer. 2024;10:103.","journal-title":"NPJ Breast Cancer"},{"key":"4150_CR67","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1186\/s13046-024-03149-x","volume":"43","author":"M Ciccioli","year":"2024","unstructured":"Ciccioli M, Kim K, Khazan N, Khoury JD, Cooke MJ, Miller MC, et al. Identification of circulating tumor cells captured by the FDA-cleared Parsortix(\u00ae) PC1 system from the peripheral blood of metastatic breast cancer patients using immunofluorescence and cytopathological evaluations. J Exp Clin Cancer Res. 2024;43:240.","journal-title":"J Exp Clin Cancer Res"},{"issue":"3","key":"4150_CR68","doi-asserted-by":"publisher","first-page":"pbad018","DOI":"10.1093\/pcmedi\/pbad018","volume":"6","author":"Q Huang","year":"2023","unstructured":"Huang Q, Zhou R, Hao X, Zhang W, Chen G, Zhu T. Circulating biomarkers in perioperative management of cancer patients. Precis Clin Med. 2023;6(3):pbad018.","journal-title":"Precis Clin Med"},{"key":"4150_CR69","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1200\/JCO.23.00456","volume":"42","author":"FC Bidard","year":"2024","unstructured":"Bidard FC, Kiavue N, Jacot W, Bachelot T, Dureau S, Bourgeois H, et al. Overall survival with circulating tumor cell count-driven choice of therapy in advanced breast cancer: a randomized trial. J Clin Oncol. 2024;42:383\u20139.","journal-title":"J Clin Oncol"},{"key":"4150_CR70","doi-asserted-by":"publisher","DOI":"10.1172\/JCI193521","author":"D Scholten","year":"2025","unstructured":"Scholten D, El-Shennawy L, Jia Y, Zhang Y, Hyun E, Reduzzi C, et al. Double-positive T cells form heterotypic clusters with circulating tumor cells to foster cancer metastasis. J Clin Invest. 2025. https:\/\/doi.org\/10.1172\/JCI193521.","journal-title":"J Clin Invest"},{"key":"4150_CR71","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23041981","author":"L Descamps","year":"2022","unstructured":"Descamps L, Le Roy D, Deman AL. Microfluidic-based technologies for CTC isolation: a review of 10 years of intense efforts towards liquid biopsy. Int J Mol Sci. 2022. https:\/\/doi.org\/10.3390\/ijms23041981.","journal-title":"Int J Mol Sci"},{"key":"4150_CR72","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1038\/s41392-023-01725-9","volume":"8","author":"Q Zhan","year":"2023","unstructured":"Zhan Q, Liu B, Situ X, Luo Y, Fu T, Wang Y, et al. New insights into the correlations between circulating tumor cells and target organ metastasis. Signal Transduct Target Ther. 2023;8:465.","journal-title":"Signal Transduct Target Ther"},{"key":"4150_CR73","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12020442","author":"C Koch","year":"2020","unstructured":"Koch C, Joosse SA, Schneegans S, Wilken OJW, Janning M, Loreth D, et al. Pre-analytical and analytical variables of label-independent enrichment and automated detection of circulating tumor cells in cancer patients. Cancers (Basel). 2020. https:\/\/doi.org\/10.3390\/cancers12020442.","journal-title":"Cancers (Basel)"}],"container-title":["Clinical and Translational Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12094-025-04150-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12094-025-04150-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12094-025-04150-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:03:17Z","timestamp":1776769397000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12094-025-04150-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":73,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["4150"],"URL":"https:\/\/doi.org\/10.1007\/s12094-025-04150-2","relation":{},"ISSN":["1699-3055"],"issn-type":[{"value":"1699-3055","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,1]]},"assertion":[{"value":"13 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.\n                      Ethical approval and informed consent\n                      This article is a narrative review and does not involve any new studies with human participants or animals performed by any of the authors. All data discussed are derived from previously published studies. Therefore, institutional ethics approval and informed consent were not required.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}