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CDT is a binary toxin comprised of an enzymatic, ADP-ribosyltransferase (ART) toxin component, CDTa, and a pore-forming or delivery subunit, CDTb. In the absence of CDTa, CDTb assembles into two distinct di-heptameric states, a symmetric and an asymmetric form with both states having two surface-accessible host cell receptor-binding domains, termed RBD1 and RBD2. RBD1 has a unique amino acid sequence, when aligned to other well-studied binary toxins (i.e., anthrax), and it contains a novel Ca<jats:sup>2+<\/jats:sup>-binding site important for CDTb stability. The other receptor binding domain, RBD2, is critically important for CDT toxicity, and a domain such as this is missing altogether in other binary toxins and shows further that CDT is unique when compared to other binary toxins. In this study, the <jats:sup>1<\/jats:sup>H, <jats:sup>13<\/jats:sup>C, and <jats:sup>15<\/jats:sup>N backbone and sidechain resonances of the 120 amino acid RBD2 domain of CDTb (residues 757\u2013876) were assigned sequence-specifically and provide a framework for future NMR-based drug discovery studies directed towards targeting the most virulent strains of CDI.<\/jats:p>","DOI":"10.1007\/s12104-020-09979-y","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T13:02:57Z","timestamp":1602248577000},"page":"35-39","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["1HN, 13C, and 15N resonance assignments of the Clostridioides difficile receptor binding domain 2 (CDTb, residues 757\u2013876)"],"prefix":"10.1007","volume":"15","author":[{"given":"Mary E.","family":"Cook","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen M.","family":"Varney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Godoy-Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8824-1110","authenticated-orcid":false,"given":"David J.","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,9]]},"reference":[{"key":"9979_CR1","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1002\/pro.2231","volume":"22","author":"N Akkaladevi","year":"2013","unstructured":"Akkaladevi N, Hinton-Chollet L, Katayama H, Mitchell J, Szerszen L, Mukherjee S, Gogol EP, Pentelute BL, Collier RJ, Fisher MT (2013) Assembly of anthrax toxin pore: lethal-factor complexes into lipid nanodiscs. 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